生物多样性 ›› 2022, Vol. 30 ›› Issue (5): 21031.  DOI: 10.17520/biods.2021031

• 研究报告: 遗传多样性 •    下一篇

基于基因组SNPs对东亚家马不同群体遗传多样性的评估

陶克涛, 白东义, 图格琴, 赵若阳, 安塔娜, 铁木齐尔·阿尔腾齐米克, 宝音德力格尔, 哈斯, 芒来(), 韩海格()   

  1. 内蒙古农业大学动物科学学院内蒙古自治区马属动物遗传育种与繁殖重点实验室, 呼和浩特 010018
  • 收稿日期:2021-07-20 接受日期:2022-04-29 出版日期:2022-05-20 发布日期:2022-05-20
  • 通讯作者: 芒来,韩海格
  • 作者简介:hanhaige@imau.edu.cn
    * E-mail: dmanglai@163.com;
  • 基金资助:
    国家自然科学基金项目(31961143025);国家自然科学基金项目(31960657);内蒙古自然基金项目(2021BS03040)

Assessment of SNPs-based genomic diversity in different populations of Eastern Asian landrace horses

Togtokh Mongke, Dongyi Bai, Tugeqin Bao, Ruoyang Zhao, Tana An, Aertengqimike Tiemuqier, Baoyindeligeer Mongkejargal, Has Soyoltiin, Manglai Dugarjaviin(), Haige Han()   

  1. Inner Mongolia Key Laboratory of Equus Genetics, Breeding and Reproduction, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018
  • Received:2021-07-20 Accepted:2022-04-29 Online:2022-05-20 Published:2022-05-20
  • Contact: Manglai Dugarjaviin,Haige Han

摘要:

家马地方品种的遗传多样性是动态演变的, 并与其育种模式息息相关; 在过去约300年中, 育种者培育了具突出表型特征的标准化培育品种, 该类型品种对家马地方品种的繁育产生了巨大影响, 这是导致家马地方品种遗传多样性下降及遗传分化的主要因素之一。本研究采集了5个东亚家马地方品种(蒙古马、哈萨克马、河曲马、藏马和西南马)、2个西亚家马品种(阿拉伯马和阿哈尔捷金马)以及2个欧洲家马品种(设特兰矮马和克莱斯黛尔马)的70个样品, 并整合之前所发表的100匹内蒙古地区家马不同群体单核苷酸多态性(SNPs)数据集, 通过全基因组重测序和生物信息学方法分析了东亚家马不同群体的遗传多样性。研究发现东亚家马地方品种具有较丰富的遗传多样性, 相比欧洲和西亚品种产生了显著的遗传分化, 其中蒙古高原群体的遗传多样性最为丰富; 受杂交改良影响, 内蒙古不同群体间产生了一定程度的遗传分化; 河曲马和藏马的遗传背景最为单一, 受引种杂交繁育影响较小。本研究评估了东亚家马地方品种的遗传多样性分布格局和演化特征, 可为建立家马地方种质核心保护群体以及培育新品种提供遗传学理论支持。

关键词: 东亚家马地方品种, 遗传多样性, 遗传分化, 引种繁育, 遗传资源保护

Abstract

Aims: The genetic diversity within landrace horse breeds changes over time and is greatly affected by breeding strategies. In the past 300 years, standardized horse breeds have been bred for prominent traits, and these breeds have been then largely introduced to improve the landrace populations which has been resulted in the reduced genetic diversity or genetic differentiation within landrace population. The aim of this study was to assess the genetic diversity of Eastern Asian landrace horse populations which will constitute the basis for the conservation of landrace horse genomic resources as well as breeding of new strain of horses.

Methods: Here we sequenced the whole genome of 70 horses from five Eastern Asian horse breeds (Hequ horse and Tibetan horse, Southwest horse, Mongolian horse, and Kazakh horse), two Western Asian breeds (Arabian horse, Akhal-Teke horse), and two European breeds (Shetland pony and Clydesdale horse). This newly generated data was combined with 100 previously published Inner Mongolian horse SNPs data to investigate the genomic diversity of the Asian landrace horse breeds.

Results: Our results suggest that the Eastern Asian landrace horse breeds have significant genetic differentiation and higher genetic diversity compared with non-Eastern Asian horse breeds. In particular, the Inner Mongolian horse populations showed the highest genetic diversity, and genetic differentiation has been observed among the populations sampled in Inner Mongolia Autonomous Region, China, which may reflect the exotic genetic influence on the genetic improving some of the local populations, such as Baicha iron hoof horse and Abag horse populations. There was no observation of this gene flow in the Hequ horse and Tibetan horse.

Conclusion: The genetic diversity of landrace horses changes and even decreases through hybridization between native and introduced modern standardized horse breeds. The wild ancestral population of domestic horses is now wholly extinct; therefore, it is imperative to maintain and protect the genetic diversity of landrace horses. The expansion of the modern standardized horse bloodlines has contributed to genetic diversity decreases and genetic differentiation increases in landrace horses. The Eastern Asian landrace horse breeds have higher genetic diversity, and endangered Baicha iron hoof horse and Abag horse have occurred significant genetic differentiation trend from the Mongolian horse population. Genetic swamping is the major concern for small population horses, therefore, the genetic conservation of Baicha iron hoof horse warrants particular attention.

Key words: Eastern Asian horse breeds, genetic diversity, genetic differentiation, introduction and breeding, genetic resources conservation