艾比湖湿地国家级自然保护区马鹿遗传多样性及遗传结构

doi:10.17520/biods.2025233

生物多样性 ›› 2025, Vol. 33 ›› Issue (12): 25233. DOI: 10.17520/biods.2025233 cstr: 32101.14.biods.2025233

• 研究报告: 遗传多样性 • 上一篇下一篇

艾比湖湿地国家级自然保护区马鹿遗传多样性及遗传结构

伍金山¹, 杨长乐², 马玉凤³, 李亚旋¹, 高文家², 叶·库斯力², 叶樑洪², 杨宇骄¹, 徐梦琦¹, 廖廷琼¹, 钟林强^1*, 单文娟^1*

1. 新疆大学生命科学与技术学院, 乌鲁木齐 830046; 2. 新疆艾比湖湿地国家级自然保护区管理局, 博乐 833400; 3. 博州哈夏国有林管理局, 温泉 833500

收稿日期:2025-06-18 修回日期:2025-11-05 接受日期:2025-12-10 出版日期:2025-12-20 发布日期:2026-01-09
通讯作者: 钟林强, 单文娟
基金资助:
艾比湖湿地国家级自然保护区2024年中央林业草原保护区级自然保护区生物多样性保护研究项目(XJGJCSCG2025-003); 国家自然科学基金地区项目(32260116)

Genetic diversity and genetic structure of red deer in the Ebinur Lake Wetland National Nature Reserve

Jinshan Wu¹, Changle Yang², Yufeng Ma³, Yaxuan Li¹, Wenjia Gao², Ye Kusili², Lianghong Ye², Yujiao Yang¹, Mengqi Xu¹, Tingqiong Liao¹, Linqiang Zhong^1*, Wenjuan Shan^1*

1 College of Life Science and Technology, Xinjiang University, Urumqi 830046

2 AibihuWelland National Nature Reserve Administration, Bole 833400

3 The Administration Bureau of the State-owned Forest in Khaxia, Bortala Prefecture,Wenquan County 833500

Received:2025-06-18 Revised:2025-11-05 Accepted:2025-12-10 Online:2025-12-20 Published:2026-01-09
Contact: Linqiang Zhong, Wenjuan Shan

摘要/Abstract

摘要： 在全球生物多样性面临威胁的背景下, 保护野生动物的遗传多样性对于维护生态平衡和提升生态系统适应能力具有至关重要的作用。本研究以艾比湖保护区马鹿为研究对象, 采用非损伤性取样法获取粪便样本, 基于微卫星和线粒体标记(CYTB、D-LOOP)分析其遗传多样性和遗传结构。结果显示, 微卫星整体观测杂合度(Ho)为0.662, 期望杂合度(He)为0.777, 多态信息含量(PIC)为0.712, 近交系数(Fis)为0.165, 整体偏离哈迪-温伯格平衡; 线粒体标记CYTB及D-LOOP单倍型多样性(Hd)分别为0.447和0.605, 核苷酸多样性(Pi)分别为0.00463和0.00334, 与其他马鹿亚种相比艾比湖保护区马鹿的遗传多样性处于中等偏高水平, 未发生瓶颈效应且存在一定程度的近交现象。微卫星Structure分析、主坐标分析表明, 艾比湖保护区马鹿分为两个聚类群体, 推测其发生了遗传分化。系统发育及遗传距离表明, 艾比湖保护区马鹿与天山马鹿及阿勒泰马鹿亲缘关系较近。作为保护区内的大型有蹄类物种之一, 其遗传多样性对维持生态系统具有重要意义。因此, 为维持保护艾比湖保护区马鹿遗传多样性, 建议加强马鹿栖息地的保护, 构建生态廊道以促进基因交流, 避免发生近交现象。

关键词: 艾比湖保护区, 马鹿, 遗传多样性, 非损伤性取样, 微卫星, 线粒体DNA

Abstract

Aims: In the context of global biodiversity facing threats, protecting the genetic diversity of wildlife is of critical importance for maintaining ecological balance and enhancing the adaptive capacity of ecosystems.

Methods: This study took red deer in the Ebinur Lake Nature Reserve as the research object, using non-invasive sampling methods to collect fecal samples, and analyzed their genetic diversity and genetic structure based on microsatellite and mitochondrial markers (CYTB, D-LOOP).

Results: The results showed that the overall observed heterozygosity (Ho) for microsatellites was 0.662, the expected heterozygosity (He) was 0.777, the polymorphic information content (PIC) was 0.712, and the inbreeding coefficient (Fis) was 0.165, indicating a deviation from Hardy-Weinberg equilibrium. The haplotype diversity (Hd) of mitochondrial markers CYTB and D-LOOP was 0.447 and 0.605, respectively, and the nucleotide diversity (Pi) was 0.00463 and 0.00334, respectively. Compared to other subspecies of red deer, the genetic diversity of red deer in Ebinur Lake National Park is moderately high. No bottleneck effect has occurred, and a certain degree of inbreeding is present. Microsatellite structure analysis and principal coordinate analysis indicate that red deer in Ebinur Lake Nature Reserve form two distinct clusters, suggesting genetic differentiation has occurred.Phylogenetic and genetic distance analysis suggests that the red deer in the Ebinur Lake Nature Reserve are closely related to the Tianshan red deer and the Altai red deer.

Conclusion: As one of the large ungulate species within the reserve, its genetic diversity is of significant importance for maintaining the ecosystem. Therefore, to preserve the genetic diversity of the red deer in the Ebinur Lake Nature Reserve, it is recommended to strengthen the protection of red deer habitats, establish ecological corridors to facilitate gene exchange, and prevent inbreeding.

Key words: Ebinur Lake Reserve, red deer, genetic diversity, non-invasive sampling, microsatellites, mitochondrial DNA

伍金山, 杨长乐, 马玉凤, 李亚旋, 高文家, 叶·库斯力, 叶樑洪, 杨宇骄, 徐梦琦, 廖廷琼, 钟林强, 单文娟 (2025) 艾比湖湿地国家级自然保护区马鹿遗传多样性及遗传结构. 生物多样性, 33, 25233. DOI: 10.17520/biods.2025233.

Jinshan Wu, Changle Yang, Yufeng Ma, Yaxuan Li, Wenjia Gao, Ye Kusili, Lianghong Ye, Yujiao Yang, Mengqi Xu, Tingqiong Liao, Linqiang Zhong, Wenjuan Shan (2025) Genetic diversity and genetic structure of red deer in the Ebinur Lake Wetland National Nature Reserve. Biodiversity Science, 33, 25233. DOI: 10.17520/biods.2025233.

导出引用管理器 EndNote|Ris|BibTeX

链接本文: https://www.biodiversity-science.net/CN/10.17520/biods.2025233

https://www.biodiversity-science.net/CN/Y2025/V33/I12/25233

[1]	周智成, 曹天玲, 刘如垚, 丁琪琪, 马轲, 杨丽萍, 周传江, 聂国兴, 汤永涛. 基于线粒体COI基因的黄河流域麦穗鱼种群遗传多样性与遗传结构[J]. 生物多样性, 2025, 33(8): 24501-.
[2]	范平, 王欢, 温知新, 宋刚, 雷富民. 气候因子对鸟类遗传多样性与物种分布面积关系的影响[J]. 生物多样性, 2025, 33(8): 25072-.
[3]	薛瑞翔, 马雪蓉, 吴炯文, 刘爱君, 张细权, 季从亮, 殷颖珊, 朱炜健, 罗庆斌. 中山麻鸭群体遗传多样性与遗传结构[J]. 生物多样性, 2025, 33(8): 24592-.
[4]	范平, 温知新, 宋刚. 气候因子和人类活动对两栖及哺乳动物不同遗传多样性指标的影响[J]. 生物多样性, 2025, 33(8): 25022-.
[5]	王儒晓, 史博洋, 潘达, 孙红英. 中国特有华溪蟹属淡水蟹多样性格局及其保护空缺[J]. 生物多样性, 2025, 33(8): 25123-.
[6]	王嘉陈, 徐汤俊, 许唯, 张高季, 尤艺瑾, 阮宏华, 刘宏毅. 城市景观格局对大蚰蜒种群遗传结构的影响[J]. 生物多样性, 2025, 33(1): 24251-.
[7]	冯晨, 张洁, 黄宏文. 统筹植物就地保护与迁地保护的解决方案: 植物并地保护(parallel situ conservation)[J]. 生物多样性, 2023, 31(9): 23184-.
[8]	李庆多, 栗冬梅. 全球蝙蝠巴尔通体流行状况分析[J]. 生物多样性, 2023, 31(9): 23166-.
[9]	齐海玲, 樊鹏振, 王跃华, 刘杰. 中国北方六省区胡桃的遗传多样性和群体结构[J]. 生物多样性, 2023, 31(8): 23120-.
[10]	熊飞, 刘红艳, 翟东东, 段辛斌, 田辉伍, 陈大庆. 基于基因组重测序的长江上游瓦氏黄颡鱼群体遗传结构[J]. 生物多样性, 2023, 31(4): 22391-.
[11]	蒲佳佳, 杨平俊, 戴洋, 陶可欣, 高磊, 杜予州, 曹俊, 俞晓平, 杨倩倩. 长江下游外来生物福寿螺的种类及其种群遗传结构[J]. 生物多样性, 2023, 31(3): 22346-.
[12]	何艺玥, 刘玉莹, 张富斌, 秦强, 曾燏, 吕振宇, 杨坤. 梯级水利工程背景下的嘉陵江干流蛇鮈群体遗传多样性和遗传结构[J]. 生物多样性, 2023, 31(11): 23160-.
[13]	孙维悦, 舒江平, 顾钰峰, 莫日根高娃, 杜夏瑾, 刘保东, 严岳鸿. 基于保护基因组学揭示荷叶铁线蕨的濒危机制[J]. 生物多样性, 2022, 30(7): 21508-.
[14]	牛晓锋, 王晓梅, 张研, 赵志鹏, 樊恩源. 鲟鱼分子鉴定方法的整合应用[J]. 生物多样性, 2022, 30(6): 22034-.
[15]	陶克涛, 白东义, 图格琴, 赵若阳, 安塔娜, 铁木齐尔·阿尔腾齐米克, 宝音德力格尔, 哈斯, 芒来, 韩海格. 基于基因组SNPs对东亚家马不同群体遗传多样性的评估[J]. 生物多样性, 2022, 30(5): 21031-.

艾比湖湿地国家级自然保护区马鹿遗传多样性及遗传结构

Genetic diversity and genetic structure of red deer in the Ebinur Lake Wetland National Nature Reserve

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价