青海湖嗜盐微生物系统发育与种群多样性

doi:10.3724/SP.J.1003.2012.10224

生物多样性 ›› 2012, Vol. 20 ›› Issue (4): 495-504. DOI: 10.3724/SP.J.1003.2012.10224

所属专题：青藏高原生物多样性与生态安全

青海湖嗜盐微生物系统发育与种群多样性

朱德锐¹^,²^,^*(), 刘建¹, 韩睿³, 沈国平², 杨芳², 龙启福², 刘德立¹

1 华中师范大学生命科学院, 湖北省遗传调控与整合生物学重点实验室, 武汉 430079
2 青海大学医学院, 西宁 810016
3 青海大学农林科学院, 西宁 810016

收稿日期:2011-12-06 接受日期:2012-05-18 出版日期:2012-07-20 发布日期:2012-09-12
通讯作者: 朱德锐
作者简介:*E-mail: zhuderui2005@126.com
基金资助:
国家自然科学基金(31060013)

Population diversity and phylogeny of halophiles in the Qinghai Lake

Derui Zhu¹^,²^,^*(), Jian Liu¹, Rui Han³, Guoping Shen², Fang Yang², Qifu Long², Deli Liu¹

1 Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Science, Central China Normal University, Wuhan 430079
2 Medical College of Qinghai University, Xining 810016
3 Qinghai Academy of Agricultural Forestry Sciences, Xining 810016

Received:2011-12-06 Accepted:2012-05-18 Online:2012-07-20 Published:2012-09-12
Contact: Derui Zhu

摘要/Abstract

摘要：

青海湖是我国境内最大的内陆咸水湖泊, 水体中嗜盐微生物的生存现状尚不明确。本研究利用OSM培养基(Oesterhelt-Stoeckenius medium), 从湖域生境水样中富集和分离获得嗜盐微生物35株, 以中度嗜盐菌为主, 约占62.9%(22株); 轻度嗜盐菌次之, 约占22.9%(8株); 耐盐菌与非嗜盐菌分别占11.4%(4株)和2.9%(1株)。根据16S rDNA序列的系统发育分析表明, γ-变形菌纲(γ-Proteobacteria)菌株最多, 约占68.6%(24株); 芽孢杆菌纲次之, 约占17.1%(6株); 放线菌纲、α-变形菌纲(α-Proteobacteria, 1株)和散囊菌亚纲(Eurotiomycetidae, 1株)的类群相对较少。这些嗜盐菌属于14个属, 其中以海洋螺菌目盐单胞菌属(Halomonas)为优势种群, 共计10株; 其次为海单胞菌属(Marinomonas), 共4株。中度嗜盐菌盐单胞菌属应为青海湖嗜盐菌的优势种群, 可能因为相对偏低的盐度环境, 为其长期进化和适应性生存提供了必要条件。

关键词: 青海湖, 嗜盐微生物, 系统发育, 种群多样性, 16S rDNA

Abstract

Qinghai Lake is the largest inland saline lake in China, but the diversity of halophiles within the lake remains unknown. We isolated 35 bacterial strains from different water samples in Qinghai Lake using an Oesterhelt-Stoeckenius medium (OSM). The majority of halophiles in this lake were moderate halophiles (grew at 0.4 to 3.5 M NaCl), accounting for about 62.9% (22 strains) of total bacteria, followed by the slight halophiles (22.9%, 8 strains) which could grow at 0 to 1.0 M NaCl, whereas the halotolerant (grew at 0 to more than 1.0 M NaCl) and nonhalophile (grew at 0 to 1.0 M NaCl) represented 11.4% (4 strains) and 2.9% (1 strain) of total bacteria, respectively. We investigated and analyzed the population diversity and evolutionary relationship based on the 16S rDNA sequences. A total of 35 bacterial strains were sequenced, and homology analysis showed that a majority was affiliated with γ-Proteobacteria and Bacilli, which accounted for 68.6% (24 strains) and 17.1% (6 strains) of total bacteria, respectively, whereas Actinobacteridae (3 strains), α-Proteobacteria (1 strain) and Eurotiomycetidae (1 strain) represented a small portion of total bacteria. Based on phylogenetics, 35 strains belonged to 14 different genera. Ten strains of Halomonas in the Oceanospirillales were the most dominant species, whereas 4 strains of Marinomonas represented a minor species component. Overall, Halomonas was the dominant group of moderate halophile, which can likely be attributed to its ability to adapt to relatively low salinity environments.

Key words: Qinghai Lake, halophile, phylogenetic evolution, population diversity, 16S rDNA

朱德锐, 刘建, 韩睿, 沈国平, 杨芳, 龙启福, 刘德立 (2012) 青海湖嗜盐微生物系统发育与种群多样性. 生物多样性, 20, 495-504. DOI: 10.3724/SP.J.1003.2012.10224.

Derui Zhu, Jian Liu, Rui Han, Guoping Shen, Fang Yang, Qifu Long, Deli Liu (2012) Population diversity and phylogeny of halophiles in the Qinghai Lake. Biodiversity Science, 20, 495-504. DOI: 10.3724/SP.J.1003.2012.10224.

导出引用管理器 EndNote|Ris|BibTeX

链接本文: https://www.biodiversity-science.net/CN/10.3724/SP.J.1003.2012.10224

https://www.biodiversity-science.net/CN/Y2012/V20/I4/495

图/表 4

参考文献 35

[1]	Cai Y (蔡艳), Xue QH (薛泉宏), Chen ZQ (陈占全), Zhang R (张荣), Lai HX (来航线) (2005) Classification and salt-resistance of Actinomycetes in the Qinghai Lake water and lakeside salinized soil. Journal of Microbiology (微生物学杂志), 25, 100-101. (in Chinese with English abstract)
[2]	Cao WJ (曹卫军), Shen P (沈萍), Li CY (李朝阳) (2004) Extremophiles 嗜极微生物. Wuhan University Press, Wuhan. (in Chinese)
[3]	Chai LH (柴丽红), Cui XL (崔晓龙), Peng Q (彭谦), Xu LH (徐丽华), Jiang CL (姜成林) (2004) Bacterial diversity of two salt lakes in Qinghai. Acta Microbiologica Sinica (微生物学报), 44, 271-275. (in Chinese with English abstract)
[4]	Chen QH (陈奇辉), Liu ZX (刘祝祥), Peng QZ (彭清忠), Huang K (黄苛), He JW (贺建武), Zhang L (张丽), Li WJ (李文均), Chen YG (陈义光) (2010) Diversity of halophilic and halotolerant bacteria isolated from non-saline soil collected from Xiaoxi national natural reserve, Hunan province. Acta Microbiologica Sinica (微生物学报), 50, 1452-1459. (in Chinese with English abstract) URL PMID
[5]	Dyall SM (2001) The Halohandbook: Protocols for Halobacterial Genetics. Melbourne Press, Melbourne.
[6]	Garrity GM, Bell JA, Lilburn T (2005) Class I Alphaproteobacteria class. nov. In: Bergey’s Manual of Systematic Bacteriology, Springer, New York.
[7]	He J (何健), Wang T (汪婷), Sun JQ (孙纪全), Gu LF (顾立锋), Li SP (李顺鹏) (2005) Isolation and characteristic of a moderately halophilic bacterium accumulated ectoine as main compatible solute. Acta Microbiologica Sinica (微生物学报), 45, 900-905. (in Chinese with English abstract) URL PMID
[8]	Horikoshi K, Antranikian G, Bull AT, Robb FT, Stetter KO (2011) Extremophiles Handbook. Springer-Verlag, New York.
[9]	Hou MF (侯梅锋), He SL (何士龙), Li D (李栋), Zhang J (张洁), Zhao Y (赵云) (2011) Bacterial diversity in Lianyungang marine sediment and Qinghai Lake sediment. Environment Science (环境科学), 32, 2681-2688. (in Chinese with English abstract)
[10]	Hou ZH (侯昭华), Xu H (徐海), An ZS (安芷生) (2009) Major ion chemistry of waters in Qinghai Lake catchment and the possible controls. Earth and Environment (地球与环境), 37, 11-19. (in Chinese with English abstract)
[11]	Jiao YC (焦迎春) (2008) Isolation and purification of psychrophile from Qinghai Lake and biochemical characteristic. Journal of Qinghai University (Nature Science) (青海大学学报(自然科学版)), 26, 11-14. (in Chinese with English abstract)
[12]	Kushner DJ (1978) Life in high salt and solute concentrations: halophilic bacteria. In: Microbial Life in Extreme Environments. Academic Press, London.
[13]	Li Q (李强), An CG (安传光), Ma Q (马强), Xu LL (徐霖林), Zhao YL (赵云龙) (2010) Species composition and diversity of zooplankton in tidal creeks of the Chongming Dongtan intertidal flat. Biodiversity Science (生物多样性), 18, 67-75. (in Chinese with English abstract)
[14]	Long QF (龙启福), Zhu DR (朱德锐), Han R (韩睿), Lu DX (芦殿香) (2011) Recent progress in researches on synthesis and transportation mechanism of compatible solutes in halophilic bacteria. Environmental Science and Technology (环境科学与技术), 34, 63-66. (in Chinese with English abstract)
[15]	Luo M (罗明), Han J (韩剑), Jiang PA (蒋平安), Wu HQ (武红旗) (2009) Diversity of culturable halophilic bacteria isolated from Lop Nur region in Xinjiang. Biodiversity Science (生物多样性), 17, 288-295. (in Chinese with English abstract)
[16]	Ma RJ (马瑞俊), Jiang ZG (蒋志刚) (2006) Impacts of environmental degradation on wild vertebrates in the Qinghai Lake drainage, China. Acta Ecologica Sinica (生态学报), 26, 3065-3073. (in Chinese with English abstract)
[17]	Mi Q (米琴), An Y (安燕), Hao W (郝微), He Y (何艳), Zhu WJ (朱文杰) (2008) Isolation and identification of luminous bacteria from the Qinghai Lake and observation by electronic microscope. Journal of East China Normal University (Natural Science) (华东师范大学学报 (自然科学版)), (4), 58-65. (in Chinese with English abstract)
[18]	Oesterhelt D, Stoeckenius W (1974) Isolation of the cell membrane of Halobacterium halobium and its fractionation into red and purple membrane. Methods in Enzymology, 31, 667-678. DOI URL PMID
[19]	Pan HL (潘海莲), Zhou C (周成), Wang HL (王红蕾), Xue YF (薛燕芬), Ma YH (马延和) (2006) Diversity of halophilic archaea in hypersaline lakes of Inner Mongolia, China. Acta Microbiologica Sinica (微生物学报), 46, 1-6. (in Chinese with English abstract) URL PMID
[20]	Sehgal SN, Gibbons NE (1960) Effect of some metal ions on the growth of Halobacterium cutirubrum. Canadian Journal of Microbiology, 6, 165-169. DOI URL PMID
[21]	Sun XX (孙鑫鑫), Liu HR (刘惠荣), Feng FY (冯福应), Meng JY (孟建宇), Li H (李蘅), Malila (玛丽娜) (2009) Diversity and phylogenetic analysis of planktonic bacteria in eutrophic zone of Lake Wuliangsuhai. Biodiversity Science (生物多样性), 17, 490-498. (in Chinese with English abstract)
[22]	Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Molecular Biology and Evolution, 24, 1596-1599. DOI URL PMID
[23]	Antonio V, Emilia Q, Francisco RV, Francisco RB, Alberto RC (1982) Numerical taxonomy of moderately halophilic gram-negative rods. Journal of General Microbiology, 128, 1959-1968.
[24]	Wang HL (王海雷), Wang YS (王云生) (2010) Preliminary analysis on Mg²+, Ca²+ and Mg/Ca as salinity indicators of lakes in the Qinghai-Tibetan Plateau. Journal of Lake Sciences (湖泊科学), 22, 894-900. (in Chinese with English abstract)
[25]	Wang JH, Tian JH, Li XY, Ma YJ, Yi WJ (2011) Evaluation of concordance between environment and economy in Qinghai Lake watershed, Qinghai-Tibet Plateau. Journal of Geographical Sciences, 21, 949-960.
[26]	Wang KR (王魁荣), Zhang SJ (张树军), Li SH (李少贺), Sang XX (桑小雪), Bai LH (白林含) (2010) Osmotolerance property and mechanism of a moderately halophilic bacterium Halomonas sp. NY-011. Chinese Journal of Applied and Environmental Biology (应用与环境生物学报), 16, 256-260. (in Chinese with English abstract)
[27]	Wei B (魏博), Wu X (吴欣) (2010) New tools for prokaryote diversity study. Chinese Journal of Ecology (生态学报), 29, 2249-2257. (in Chinese with English abstract)
[28]	Wu HP (吴海平), Wang ZH (王真辉), Yang LF (杨礼富) (2010) The selective enrichment and diversity analysis of halophilic bacteria in sedimental sample from Daban Salt Lake in Xinjiang. Microbiology China (微生物学通报), 37, 956-961. (in Chinese with English abstract)
[29]	Xie ZL (谢占玲), Wang H (王欢), Zhao P (赵朋), Yu J (余静), Lü C (吕超), Shen XH (申贤慧), Gong BL (龚宝林), Liu Y (刘英) (2012) Diversity of halotolerant fungi isolated from Qinghai Lake based on culture-dependent investigation. Mycosystema (菌物学报), 31, 187-195. (in Chinese with English abstract)
[30]	Xu HS, Roberts N, Singleton FL, Attwell RW, Grimes DJ, Colwel RR (1982) Survival and viability of nonculturable Escherichia coli and Vibrio cholerar in the estuarine and marine environment. Microbial Ecology, 8, 313-323. DOI URL PMID
[31]	Yang BH (杨博辉), Lang X (郎侠), Sun XP (孙晓萍) (2005) Biodiversity on Qinghai-Tibet Plateau. Ecology of Domestic Animal (家畜生态学报), 26, 1-5. (in Chinese with English abstract)
[32]	Zhang EL (张恩楼), Shen J (沈吉), Wang SM (王苏民), Yin Y (尹宇), Zhu YX (朱育新), Xia WL (夏威岚) (2004) Quantitative reconstruction of the paleosalinity at Qinghai Lake in the past 900 years. Chinese Science Bulletin (科学通报), 49, 730-734. (in Chinese with English abstract)
[33]	Zhang K (张琨), Lan JH (蓝江湖), Shen ZX (沈振兴), Xu H (徐海) (2010) The chemical composition and quality evaluation of surface water in Qinghai Lake areas. Journal of Earth Environment (地球环境学报), 1, 162-168. (in Chinese with English abstract)
[34]	Zhang XH (张现辉), Kong FJ (孔凡晶) (2010) Bacterial diversity in Zabuye Salt Lake of Tibet by culture-independent approaches. Acta Microbiologica Sinica (微生物学报), 50, 334-341. (in Chinese with English abstract) URL PMID
[35]	Zhu WJ (朱文杰), Wang J (汪杰), Chen XY (陈晓耘), Zhaxi C (扎西次仁), Yang Y (杨韵), Song Y (宋瑛) (1994) A new species of luminous bacteria Vibrio qinghaiensis sp. nov. Oceanologia et Limnologia Sinica (海洋与湖沼), 25, 273-280. (in Chinese with English abstract)

嗜盐微生物类型 Halophile type	生长盐度 Growth salinity (M)	最适盐度 Optimal growth salinity (M)	菌株编号 Strain no.
非嗜盐 Nonhalophile	0-1.0	< 0.2	QHL29
轻度嗜盐 Slight halophile	0.2-2.0	0.2-0.5	QHL13、QHL20、QHL22、QHL27、QHL30、QHL32、QHL33、QHL34
中度嗜盐 Moderate halophile	0.4-3.5	0.5-2.0	QHL1、QHL2、QHL3、QHL4、QHL5、QHL6、QHL8、QHL9、QHL10、QHL11、QHL14、QHL15、QHL16、QHL17、QHL18、QHL19、QHL23、QHL24、QHL25、QHL26、QHL28、QHL35
边界极端嗜盐 Bordline extreme halophile	1.4-3.5	2.0-3.0
极端嗜盐 Extreme halophile	2.0-5.2	>3.0
耐盐 Halotolerant	0->1.0	<0.2	QHL7、QHL12、QHL21、QHL31
万能耐盐 Haloversatile	0->3.0	0.2-0.5

嗜盐微生物类型 Halophile type	生长盐度 Growth salinity (M)	最适盐度 Optimal growth salinity (M)	菌株编号 Strain no.
非嗜盐 Nonhalophile	0-1.0	< 0.2	QHL29
轻度嗜盐 Slight halophile	0.2-2.0	0.2-0.5	QHL13、QHL20、QHL22、QHL27、QHL30、QHL32、QHL33、QHL34
中度嗜盐 Moderate halophile	0.4-3.5	0.5-2.0	QHL1、QHL2、QHL3、QHL4、QHL5、QHL6、QHL8、QHL9、QHL10、QHL11、QHL14、QHL15、QHL16、QHL17、QHL18、QHL19、QHL23、QHL24、QHL25、QHL26、QHL28、QHL35
边界极端嗜盐 Bordline extreme halophile	1.4-3.5	2.0-3.0
极端嗜盐 Extreme halophile	2.0-5.2	>3.0
耐盐 Halotolerant	0->1.0	<0.2	QHL7、QHL12、QHL21、QHL31
万能耐盐 Haloversatile	0->3.0	0.2-0.5

菌株编号 Strains no.	GenBank中同源菌株 Homologous strains from GenBank	相似度 Identity (%)	分类学地位 Phylogenetic classification
QHL1	Halomonas sp. TNB I58 (AB166899)	99	变形菌门; γ-变形菌纲; 海洋螺菌目; 盐单胞菌科; 盐单胞菌属 Proteobacteria; γ-Proteobacteria; Oceanospirillales; Halomonadaceae; Halomonas
QHL2	Vibrio diazotrophicus N6 (JF775501)	99	变形菌门; γ-变形菌纲; 弧菌目; 弧菌科; 弧菌属 Proteobacteria; γ-Proteobacteria; Vibrionales; Vibrionaceae; Vibrio
QHL3	Vibrio sp. CCMM B663 (FR695477)	99	变形菌门; γ-变形菌纲; 弧菌目; 弧菌科; 弧菌属 Proteobacteria; γ-Proteobacteria; Vibrionales; Vibrionaceae; Vibrio
QHL4	Halomonas ventosae (JF420893)	99	变形菌门; γ-变形菌纲; 海洋螺菌目; 盐单胞菌科; 盐单胞菌属 Proteobacteria; γ-Proteobacteria; Oceanospirillales; Halomonadaceae; Halomonas
QHL5	Halomonas ventosae (GQ903444)	99	变形菌门; γ-变形菌纲; 海洋螺菌目; 盐单胞菌科; 盐单胞菌属 Proteobacteria; γ-Proteobacteria; Oceanospirillales; Halomonadaceae; Halomonas
QHL6	Halomonas ventosae (JF420893)	99	变形菌门; γ-变形菌纲; 海洋螺菌目; 盐单胞菌科; 盐单胞菌属 Proteobacteria; γ-Proteobacteria; Oceanospirillales; Halomonadaceae; Halomonas
QHL7	Exiguobacterium sp. (DQ019169)	99	厚壁菌门; 芽孢杆菌纲; 芽孢杆菌目; 芽孢杆菌科; 微小杆菌属 Firmicutes; Bacilli; Bacillales; Bacillaceae; Exiguobacterium
QHL8	Halomonas sp. TNB I58 (AB166899)	98	变形菌门; γ-变形菌纲; 海洋螺菌目; 盐单胞菌科; 盐单胞菌属 Proteobacteria; γ-Proteobacteria; Oceanospirillales; Halomonadaceae; Halomonas
QHL9	Oceanobacillus picturae (AY505535)	99	厚壁菌门; 芽孢杆菌纲; 芽孢杆菌目; 芽孢杆菌科; 大洋短杆菌属 Firmicutes; Bacilli; Bacillales; Bacillaceae; Oceanobacillus
QHL10	Brevibacterium sp. (GQ199723)	99	放线菌门; 放线菌亚纲; 放线菌目; 短杆菌科; 短杆菌属 Actinobacteria; Actinobacteridae; Actinomycetales; Brevibacteriaceae; Brevibacterium
QHL11	Oceanobacillus picturae (JF411375)	99	厚壁菌门; 芽孢杆菌纲; 芽孢杆菌目; 芽孢杆菌科; 大洋短杆菌属 Firmicutes; Bacilli; Bacillales; Bacillaceae; Oceanobacillus
QHL12	Arthrobacter sp. TM4(DQ279376)	99	放线菌门; 放线菌亚纲; 微球菌亚目; 微球菌科; 短杆菌属 Actinomycetales; Actinobacteridae; Micrococcineae; Micrococcaceae; Arthrobacter
QHL13	Marinomonas sp.(DQ492756)	100	变形菌门; γ-变形菌纲; 海洋螺菌目; 海洋螺菌科; 海单胞菌属 Proteobacteria; γ-Proteobacteria; Oceanospirillales; Oceanospirillaaceae; Marinomonas
QHL14	Planococcus maritimus (AY582938)	99	厚壁菌门; 芽孢杆菌纲; 芽孢杆菌目; 动性球菌科; 动性球菌属 Firmicutes; Bacilli; Bacillales; Planococcaceae; Planococcus
QHL15^*	Aspergillus sp. (FJ654485)	99	真菌; 子囊菌门; 散囊菌亚纲; 散囊菌目; 发菌科; 曲霉属; Fungi; Ascomycota; Eurotiomycetidae; Eurotiales; Trichocomaceae; Aspergillus
QHL16	Halomonas sp. (JN195791)	99	变形菌门; γ-变形菌纲; 海洋螺菌目; 盐单胞菌科; 盐单胞菌属 Proteobacteria; γ-Proteobacteria; Oceanospirillales; Halomonadaceae; Halomonas
QHL17	Planococcus rifietoensis (NR025553)	100	厚壁菌门; 芽孢杆菌纲; 芽孢杆菌目; 动性球菌科; 动性球菌属 Firmicutes; Bacilli; Bacillales; Planococcaceae; Planococcus
QHL18	Halomonas sp. K1B-11 (FJ889578)	99	变形菌门; γ-变形菌纲; 海洋螺菌目; 盐单胞菌科; 盐单胞菌属 Proteobacteria; γ-Proteobacteria; Oceanospirillales; Halomonadaceae; Halomonas
QHL19	Vibrio metschnikovii (EF645831)	98	变形菌门; γ-变形菌纲; 弧菌目; 弧菌科; 弧菌属 Proteobacteria; γ-Proteobacteria; Vibrionales; Vibrionaceae; Vibrio
QHL20	Alteromonas marina (AY881234)	98	变形菌门; γ-变形菌纲; 交替单胞菌目; 交替单胞菌科; 交替单胞菌属 Proteobacteria; γ-Proteobacteria; Alteromonadales; Alteromonadaceae; Alteromonas
QHL21	Acinetobacter junii (AM184279)	99	变形菌门; γ-变形菌纲; 假单胞菌目; 莫拉菌科; 不动杆菌属 Proteobacteria; γ-Proteobacteria; Pseudomonadales; Moraxellaceae; Acinetobacter
QHL22	Pseudomonas sp. Ebht4 (JF895528)	99	变形菌门; γ-变形菌纲; 假单胞菌目; 假单胞菌科; 假单胞菌属 Proteobacteria; γ-Proteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas
QHL23	Alteromonas sp. (AM990853)	99	变形菌门; γ-变形菌纲; 交替单胞菌目; 交替单胞菌科; 交替单胞菌属 Proteobacteria; γ-Proteobacteria; Alteromonadales; Alteromonadaceae; Alteromonas
QHL24	Planococcus sp. CU6 (DQ643135)	99	厚壁菌门; 芽孢杆菌纲; 芽孢杆菌目; 动性球菌科; 动性球菌属 Firmicutes; Bacilli; Bacillales; Planococcaceae; Planococcus
QHL25	Halomonas sp. K1B-11 (FJ889578)	98	变形菌门; γ-变形菌纲; 海洋螺菌目; 盐单胞菌科; 盐单胞菌属 Proteobacteria; γ-Proteobacteria; Oceanospirillales; Halomonadaceae; Halomonas
QHL26	Halomonas sp. K1B-11 (FJ889578)	99	变形菌门; γ-变形菌纲; 海洋螺菌目; 盐单胞菌科; 盐单胞菌属 Proteobacteria; γ-Proteobacteria; Oceanospirillales; Halomonadaceae; Halomonas
QHL27	Marinomonas sp. BSi20337 (DQ492756)	99	变形菌门; γ-变形菌纲; 海洋螺菌目; 海洋螺菌科; 海单胞菌属 Proteobacteria; γ-Proteobacteria; Oceanospirillales; Oceanospirillaaceae; Marinomonas
QHL28	Marinomonas sp. BSi20337 (DQ492756)	99	变形菌门; γ-变形菌纲; 海洋螺菌目; 海洋螺菌科; 海单胞菌属 Proteobacteria; γ-Proteobacteria; Oceanospirillales; Oceanospirillaaceae; Marinomonas
QHL29	Halomonas sp. (AB305227)	99	变形菌门; γ-变形菌纲; 海洋螺菌目; 盐单胞菌科; 盐单胞菌属 Proteobacteria; γ-Proteobacteria; Oceanospirillales; Halomonadaceae; Halomonas
QHL30	Pseudomonas mendocina (JF895528)	99	变形菌门; γ-变形菌纲; 假单胞菌目; 假单胞菌科; 假单胞菌属 Proteobacteria; γ-Proteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas
QHL31	Sphingomonas sp. SBK-16 (AB366357)	99	变形菌门; γ-变形菌纲; 鞘氨醇单胞菌目; 鞘氨醇单胞菌科; 鞘氨醇单胞菌属 Proteobacteria; α-Proteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingomonas
QHL32	Jonesia quinghaiensis (NR029030)	99	放线菌门; 放线菌亚纲; 微球菌亚目; 琼氏菌科; 琼氏菌属 Actinomycetales; Actinobacteridae; Micrococcineae; Jonesiaceae; Jonesia
QHL33	Pseudomonas sp. HNS021 (JN128255)	99	变形菌门; γ-变形菌纲; 假单胞菌目; 假单胞菌科; 假单胞菌属 Proteobacteria; γ-Proteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas
QHL34	Marinomonas sp. HDW-15 (JN087530)	98	变形菌门; γ-变形菌纲; 海洋螺菌目; 海洋螺菌科; 海单胞菌属 Proteobacteria; γ-Proteobacteria; Oceanospirillales; Oceanospirillaaceae; Marinomonas
QHL35	Marinobacter sp. (AY588955)	99	变形菌门; γ-变形菌纲; 交替单胞菌目; 交替单胞菌科; 海杆菌属 Proteobacteria; γ-Proteobacteria; Alteromonadales; Alteromonadaceae; Marinobacter

菌株编号 Strains no.	GenBank中同源菌株 Homologous strains from GenBank	相似度 Identity (%)	分类学地位 Phylogenetic classification
QHL1	Halomonas sp. TNB I58 (AB166899)	99	变形菌门; γ-变形菌纲; 海洋螺菌目; 盐单胞菌科; 盐单胞菌属 Proteobacteria; γ-Proteobacteria; Oceanospirillales; Halomonadaceae; Halomonas
QHL2	Vibrio diazotrophicus N6 (JF775501)	99	变形菌门; γ-变形菌纲; 弧菌目; 弧菌科; 弧菌属 Proteobacteria; γ-Proteobacteria; Vibrionales; Vibrionaceae; Vibrio
QHL3	Vibrio sp. CCMM B663 (FR695477)	99	变形菌门; γ-变形菌纲; 弧菌目; 弧菌科; 弧菌属 Proteobacteria; γ-Proteobacteria; Vibrionales; Vibrionaceae; Vibrio
QHL4	Halomonas ventosae (JF420893)	99	变形菌门; γ-变形菌纲; 海洋螺菌目; 盐单胞菌科; 盐单胞菌属 Proteobacteria; γ-Proteobacteria; Oceanospirillales; Halomonadaceae; Halomonas
QHL5	Halomonas ventosae (GQ903444)	99	变形菌门; γ-变形菌纲; 海洋螺菌目; 盐单胞菌科; 盐单胞菌属 Proteobacteria; γ-Proteobacteria; Oceanospirillales; Halomonadaceae; Halomonas
QHL6	Halomonas ventosae (JF420893)	99	变形菌门; γ-变形菌纲; 海洋螺菌目; 盐单胞菌科; 盐单胞菌属 Proteobacteria; γ-Proteobacteria; Oceanospirillales; Halomonadaceae; Halomonas
QHL7	Exiguobacterium sp. (DQ019169)	99	厚壁菌门; 芽孢杆菌纲; 芽孢杆菌目; 芽孢杆菌科; 微小杆菌属 Firmicutes; Bacilli; Bacillales; Bacillaceae; Exiguobacterium
QHL8	Halomonas sp. TNB I58 (AB166899)	98	变形菌门; γ-变形菌纲; 海洋螺菌目; 盐单胞菌科; 盐单胞菌属 Proteobacteria; γ-Proteobacteria; Oceanospirillales; Halomonadaceae; Halomonas
QHL9	Oceanobacillus picturae (AY505535)	99	厚壁菌门; 芽孢杆菌纲; 芽孢杆菌目; 芽孢杆菌科; 大洋短杆菌属 Firmicutes; Bacilli; Bacillales; Bacillaceae; Oceanobacillus
QHL10	Brevibacterium sp. (GQ199723)	99	放线菌门; 放线菌亚纲; 放线菌目; 短杆菌科; 短杆菌属 Actinobacteria; Actinobacteridae; Actinomycetales; Brevibacteriaceae; Brevibacterium
QHL11	Oceanobacillus picturae (JF411375)	99	厚壁菌门; 芽孢杆菌纲; 芽孢杆菌目; 芽孢杆菌科; 大洋短杆菌属 Firmicutes; Bacilli; Bacillales; Bacillaceae; Oceanobacillus
QHL12	Arthrobacter sp. TM4(DQ279376)	99	放线菌门; 放线菌亚纲; 微球菌亚目; 微球菌科; 短杆菌属 Actinomycetales; Actinobacteridae; Micrococcineae; Micrococcaceae; Arthrobacter
QHL13	Marinomonas sp.(DQ492756)	100	变形菌门; γ-变形菌纲; 海洋螺菌目; 海洋螺菌科; 海单胞菌属 Proteobacteria; γ-Proteobacteria; Oceanospirillales; Oceanospirillaaceae; Marinomonas
QHL14	Planococcus maritimus (AY582938)	99	厚壁菌门; 芽孢杆菌纲; 芽孢杆菌目; 动性球菌科; 动性球菌属 Firmicutes; Bacilli; Bacillales; Planococcaceae; Planococcus
QHL15^*	Aspergillus sp. (FJ654485)	99	真菌; 子囊菌门; 散囊菌亚纲; 散囊菌目; 发菌科; 曲霉属; Fungi; Ascomycota; Eurotiomycetidae; Eurotiales; Trichocomaceae; Aspergillus
QHL16	Halomonas sp. (JN195791)	99	变形菌门; γ-变形菌纲; 海洋螺菌目; 盐单胞菌科; 盐单胞菌属 Proteobacteria; γ-Proteobacteria; Oceanospirillales; Halomonadaceae; Halomonas
QHL17	Planococcus rifietoensis (NR025553)	100	厚壁菌门; 芽孢杆菌纲; 芽孢杆菌目; 动性球菌科; 动性球菌属 Firmicutes; Bacilli; Bacillales; Planococcaceae; Planococcus
QHL18	Halomonas sp. K1B-11 (FJ889578)	99	变形菌门; γ-变形菌纲; 海洋螺菌目; 盐单胞菌科; 盐单胞菌属 Proteobacteria; γ-Proteobacteria; Oceanospirillales; Halomonadaceae; Halomonas
QHL19	Vibrio metschnikovii (EF645831)	98	变形菌门; γ-变形菌纲; 弧菌目; 弧菌科; 弧菌属 Proteobacteria; γ-Proteobacteria; Vibrionales; Vibrionaceae; Vibrio
QHL20	Alteromonas marina (AY881234)	98	变形菌门; γ-变形菌纲; 交替单胞菌目; 交替单胞菌科; 交替单胞菌属 Proteobacteria; γ-Proteobacteria; Alteromonadales; Alteromonadaceae; Alteromonas
QHL21	Acinetobacter junii (AM184279)	99	变形菌门; γ-变形菌纲; 假单胞菌目; 莫拉菌科; 不动杆菌属 Proteobacteria; γ-Proteobacteria; Pseudomonadales; Moraxellaceae; Acinetobacter
QHL22	Pseudomonas sp. Ebht4 (JF895528)	99	变形菌门; γ-变形菌纲; 假单胞菌目; 假单胞菌科; 假单胞菌属 Proteobacteria; γ-Proteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas
QHL23	Alteromonas sp. (AM990853)	99	变形菌门; γ-变形菌纲; 交替单胞菌目; 交替单胞菌科; 交替单胞菌属 Proteobacteria; γ-Proteobacteria; Alteromonadales; Alteromonadaceae; Alteromonas
QHL24	Planococcus sp. CU6 (DQ643135)	99	厚壁菌门; 芽孢杆菌纲; 芽孢杆菌目; 动性球菌科; 动性球菌属 Firmicutes; Bacilli; Bacillales; Planococcaceae; Planococcus
QHL25	Halomonas sp. K1B-11 (FJ889578)	98	变形菌门; γ-变形菌纲; 海洋螺菌目; 盐单胞菌科; 盐单胞菌属 Proteobacteria; γ-Proteobacteria; Oceanospirillales; Halomonadaceae; Halomonas
QHL26	Halomonas sp. K1B-11 (FJ889578)	99	变形菌门; γ-变形菌纲; 海洋螺菌目; 盐单胞菌科; 盐单胞菌属 Proteobacteria; γ-Proteobacteria; Oceanospirillales; Halomonadaceae; Halomonas
QHL27	Marinomonas sp. BSi20337 (DQ492756)	99	变形菌门; γ-变形菌纲; 海洋螺菌目; 海洋螺菌科; 海单胞菌属 Proteobacteria; γ-Proteobacteria; Oceanospirillales; Oceanospirillaaceae; Marinomonas
QHL28	Marinomonas sp. BSi20337 (DQ492756)	99	变形菌门; γ-变形菌纲; 海洋螺菌目; 海洋螺菌科; 海单胞菌属 Proteobacteria; γ-Proteobacteria; Oceanospirillales; Oceanospirillaaceae; Marinomonas
QHL29	Halomonas sp. (AB305227)	99	变形菌门; γ-变形菌纲; 海洋螺菌目; 盐单胞菌科; 盐单胞菌属 Proteobacteria; γ-Proteobacteria; Oceanospirillales; Halomonadaceae; Halomonas
QHL30	Pseudomonas mendocina (JF895528)	99	变形菌门; γ-变形菌纲; 假单胞菌目; 假单胞菌科; 假单胞菌属 Proteobacteria; γ-Proteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas
QHL31	Sphingomonas sp. SBK-16 (AB366357)	99	变形菌门; γ-变形菌纲; 鞘氨醇单胞菌目; 鞘氨醇单胞菌科; 鞘氨醇单胞菌属 Proteobacteria; α-Proteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingomonas
QHL32	Jonesia quinghaiensis (NR029030)	99	放线菌门; 放线菌亚纲; 微球菌亚目; 琼氏菌科; 琼氏菌属 Actinomycetales; Actinobacteridae; Micrococcineae; Jonesiaceae; Jonesia
QHL33	Pseudomonas sp. HNS021 (JN128255)	99	变形菌门; γ-变形菌纲; 假单胞菌目; 假单胞菌科; 假单胞菌属 Proteobacteria; γ-Proteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas
QHL34	Marinomonas sp. HDW-15 (JN087530)	98	变形菌门; γ-变形菌纲; 海洋螺菌目; 海洋螺菌科; 海单胞菌属 Proteobacteria; γ-Proteobacteria; Oceanospirillales; Oceanospirillaaceae; Marinomonas
QHL35	Marinobacter sp. (AY588955)	99	变形菌门; γ-变形菌纲; 交替单胞菌目; 交替单胞菌科; 海杆菌属 Proteobacteria; γ-Proteobacteria; Alteromonadales; Alteromonadaceae; Marinobacter

青海湖嗜盐微生物系统发育与种群多样性

Population diversity and phylogeny of halophiles in the Qinghai Lake

RichHTML

PDF (PC)

可视化

被引次数

摘要/Abstract

引用本文

使用本文

图/表 4

参考文献 35

相关文章 15

编辑推荐

Metrics

本文评价

[1]	艾妍雨, 胡海霞, 沈婷, 莫雨轩, 杞金华, 宋亮. 附生维管植物多样性及其与宿主特征的相关性: 以哀牢山中山湿性常绿阔叶林为例[J]. 生物多样性, 2024, 32(5): 24072-.
[2]	吕燕文, 王子韵, 肖钰, 何梓晗, 吴超, 胡新生. 谱系分选理论与检测方法的研究进展[J]. 生物多样性, 2024, 32(4): 23400-.
[3]	曹可欣, 王敬雯, 郑国, 武鹏峰, 李英滨, 崔淑艳. 降水格局改变及氮沉降对北方典型草原土壤线虫多样性的影响[J]. 生物多样性, 2024, 32(3): 23491-.
[4]	李庆多, 栗冬梅. 全球蝙蝠巴尔通体流行状况分析[J]. 生物多样性, 2023, 31(9): 23166-.
[5]	李治中, 彭帅, 王青锋, 李伟, 梁士楚, 陈进明. 中国海菜花属植物隐种多样性[J]. 生物多样性, 2023, 31(2): 22394-.
[6]	宋会银, 胡征宇, 刘国祥. 绿藻门小球藻科的分类学研究进展[J]. 生物多样性, 2023, 31(2): 22083-.
[7]	朱瑞良, 马晓英, 曹畅, 曹子寅. 中国苔藓植物多样性研究进展[J]. 生物多样性, 2022, 30(7): 22378-.
[8]	王婷, 舒江平, 顾钰峰, 李艳清, 杨拓, 徐洲锋, 向建英, 张宪春, 严岳鸿. 中国石松类和蕨类植物多样性研究进展[J]. 生物多样性, 2022, 30(7): 22381-.
[9]	涂文娜, 易嘉伟, 杜云艳, 王楠, 千家乐, 黄胜, 王晓悦. 青海湖自然保护区人类数字足迹及草地生物量的人类活动暴露度的时空模式分析[J]. 生物多样性, 2022, 30(6): 21310-.
[10]	王健铭, 曲梦君, 王寅, 冯益明, 吴波, 卢琦, 何念鹏, 李景文. 青藏高原北部戈壁植物群落物种、功能与系统发育β多样性分布格局及其影响因素[J]. 生物多样性, 2022, 30(6): 21503-.
[11]	姜晓燕, 高圣杰, 蒋燕, 田赟, 贾昕, 查天山. 毛乌素沙地植被不同恢复阶段植物群落物种多样性、功能多样性和系统发育多样性[J]. 生物多样性, 2022, 30(5): 21387-.
[12]	赵琦, 蒋际宝, 张曾鲁, 金清, 李佳丽, 邱江平. 海南岛蚯蚓物种组成及其系统发育分析[J]. 生物多样性, 2022, 30(12): 22224-.
[13]	栗冬梅, 杨卫红, 李庆多, 韩茜, 宋秀平, 潘虹, 冯云. 巴尔通体在滇西南蝙蝠中高度流行并具有丰富的遗传变异特征[J]. 生物多样性, 2021, 29(9): 1245-1255.
[14]	陈旸康, 王益, 李家亮, 王文韬, 冯端宇, 毛康珊. 主流分子钟定年方法的原理、误差来源和使用建议[J]. 生物多样性, 2021, 29(5): 629-646.
[15]	陈博, 江蓝, 谢子扬, 李阳娣, 李佳萱, 李梦佳, 魏晨思, 邢聪, 刘金福, 何中声. 格氏栲天然林林窗植物物种多样性与系统发育多样性[J]. 生物多样性, 2021, 29(4): 439-448.