中国海菜花属植物隐种多样性

doi:10.17520/biods.2022394

生物多样性 ›› 2023, Vol. 31 ›› Issue (2): 22394. DOI: 10.17520/biods.2022394

• 研究报告: 植物多样性 • 上一篇下一篇

中国海菜花属植物隐种多样性

李治中¹^,², 彭帅³^,⁴, 王青锋³^,⁵, 李伟¹, 梁士楚²^,^*(), 陈进明¹^,^*()

1.中国科学院武汉植物园水生植物研究中心, 武汉 430074
2.广西师范大学珍稀濒危动植物生态与环境保护教育部重点实验室, 广西桂林 541006
3.中国科学院武汉植物园植物多样性研究中心, 武汉 430074
4.中国科学院大学, 北京 100049
5.中国科学院中-非联合研究中心, 武汉 430074

收稿日期:2022-07-11 接受日期:2022-09-05 出版日期:2023-02-20 发布日期:2022-09-19
通讯作者: *陈进明, E-mail: jmchen@wbgcas.cn;梁士楚 gxlsc@sina.com
基金资助:
国家自然科学基金(32100186);国家自然科学基金(32070231);国家自然科学基金(32120103002);广西师范大学珍稀濒危动植物生态与环境保护教育部重点实验室研究基金(ERESEP2022K03);中国科学院大学生科研实践训练项目(20214002740)

Cryptic diversity of the genus Ottelia in China

Zhizhong Li¹^,², Shuai Peng³^,⁴, Qingfeng Wang³^,⁵, Wei Li¹, Shichu Liang²^,^*(), Jinming Chen¹^,^*()

1. Aquatic Plant Research Center, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074
2. Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, Guilin, Guangxi 541006
3. Plant Biodiversity Research Center, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074
4. University of Chinese Academy of Sciences, Beijing 100049
5. Sino-Africa Joint Research Center, Chinese Academy of Sciences, Wuhan 430074

Received:2022-07-11 Accepted:2022-09-05 Online:2023-02-20 Published:2022-09-19
Contact: *Jinming Chen, E-mail: jmchen@wbgcas.cn;Shichu Liang gxlsc@sina.com

摘要/Abstract

摘要：

近20年来, 随着分子生物学的兴起, 越来越多的证据表明生命之树的各个分支中都存在大量的隐种多样性, 占据未被发现的生物多样性的极高比例。准确地评估濒危类群的隐种多样性水平, 不仅能更好地理解物种形成在生物多样性式样及过程中的异质性作用, 而且也更有利于生物多样性的有效保护及管理。海菜花属(Ottelia)是我国二级重点保护植物, 为泛热带水生植物类群, 我国云贵高原及其邻近区域为该属重要的物种多样性中心之一。由于该属具有高度的表型可塑性, 缺少明确的鉴别特征, 致使其属下分类一直未能很好解决, 这极大限制了相关保护措施的制定。因此, 本研究在对我国海菜花属植物广泛采样的基础上, 利用多位点联合贝叶斯方法对其进行物种划分研究。结果表明: 我国海菜花属植物至少包括14种, 分子证据支持将海菜花种下3个变种提升为种, 同时海菜花(O. acuminata)和靖西海菜花(O. jingxiensis)内存在隐种现象。喀斯特地区的异质性生境及西南地区水系间的地理隔离可能是我国海菜花属特有类群快速分化的主要原因。基于本研究结果, 我们建议将各分类群(包括存疑类群)分别作为独立单元就地保护。

关键词: 海菜花, 物种划分, 系统发育, 分类学处理

Abstract

Aims: In the past 20 years, with the rise of molecular biology, there is more and more evidence that extensive cryptic species occupy a high proportion of undiscovered biodiversity. Accurate assessment of endangered taxa diversity is beneficial to better understand the speciation in biodiversity patterns and the process of heterogeneity. It can also contribute to the effective protection and management of biodiversity. Ottelia, grade II plants in the List of the National Key Protected Wild Plants in China, is a pan-tropical aquatic plant group, the Yunnan-Guizhou Plateau and its adjacent areas is an important centre of species diversity in the genus. Due to its high phenotypic plasticity and lack of unambiguous identification characteristics, the taxonomy of the genus has not been well described, greatly limiting the formulation of relevant protections.

Methods: Based on extensive sampling of Ottelia populations in China, we used a Bayesian coalescent method for species delimitation using the multi-locus data set (8 plastid regions and nrITS).

Results: We showed with molecular evidence that in at least 14 Ottelia species in China, all three varieties of O. acuminata should be treated at the species level. Also, there were cryptic species found in O. acuminata and O. jingxiensis. We speculate that the heterogeneous habitats in the karst region and the geographical isolation between the water systems in southwest China may explain the rapid speciation of endemic Ottelia taxa in China.

Conclusion: According to our results, we propose that each taxon (including cryptic species) should be protected as independent protected units.

Key words: Ottelia acuminata, species delimitation, phylogeny, taxonomic treatment

李治中, 彭帅, 王青锋, 李伟, 梁士楚, 陈进明 (2023) 中国海菜花属植物隐种多样性. 生物多样性, 31, 22394. DOI: 10.17520/biods.2022394.

Zhizhong Li, Shuai Peng, Qingfeng Wang, Wei Li, Shichu Liang, Jinming Chen (2023) Cryptic diversity of the genus Ottelia in China. Biodiversity Science, 31, 22394. DOI: 10.17520/biods.2022394.

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链接本文: https://www.biodiversity-science.net/CN/10.17520/biods.2022394

https://www.biodiversity-science.net/CN/Y2023/V31/I2/22394

图/表 4

图1 本研究海菜花属居群采样点地图。居群和个体信息详见附录1。

Fig. 1 Map of sampled population site of Ottelia in this study. Information on populations and the individuals see Appendix 1.

图2 基于叶绿体片段(a)及nrITS (b)对海菜花属植物构建的系统发育树。贝叶斯推理后验概率(PP)大于0.70及最大似然法分析支持度(BS)大于70展示在节点上。使用的物种、居群和个体信息见附录1, 水筛(Blyxa japonica)为外类群。

Fig. 2 Phylogenetic tree of Ottelia using ptDNA (a) and nrITS (b), respectively. Posterior probabilities (PP > 0.70) and bootstrap values (BS > 70) based on Bayesian Inference (BI) and Maximum Likelihood (ML) analysis are shown at nodes. Information on the individual code, population code, and species see Appendix 1. Blyxa japonica was included as outgroup.

图3 基于多位点(8个叶绿体片段及nrITS)数据集最大置信树及STACEY结果。仅展示后验概率高于0.7的节点。黑、白阵列方格分别表示相似值1和0。使用的物种、居群和个体信息见附录1。

Fig. 3 Maximum credibility tree from STACEY analysis in BEAST2 based on multi-locus (nrITS and ptDNA). Only posterior probabilities higher than 0.7 were shown at nodes. Black and white matrix squares indicated posterior probabilities of 1 and 0, respectively. Information on the individual code, population code, and species see Appendix 1.

图4 本研究中4个海菜花属存疑类群。A?E: Ottelia sp. HCH72; F?G: Ottelia sp. 35; H?I: Ottelia sp. 30; J?L: Ottelia sp. OTT13。

Fig. 4 Four doubtful taxa in this study. A?E, Ottelia sp. HCH72; F?G, Ottelia sp. 35; H?I: Ottelia sp. 30; J?L, Ottelia sp. OTT13.

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中国海菜花属植物隐种多样性

Cryptic diversity of the genus Ottelia in China

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