生物多样性 ›› 2012, Vol. 20 ›› Issue (6): 693-702.DOI: 10.3724/SP.J.1003.2012.10062

• 研究报告 • 上一篇    下一篇

利用SSR标记分析茄镰孢豌豆专化型的遗传多样性

向妮1,2, 肖炎农2, 段灿星1, 王晓鸣1, 朱振东1,*()   

  1. 1 中国农业科学院作物科学研究所, 农作物基因资源与基因改良国家重大科学工程, 北京 100081
    2 华中农业大学植物科技学院, 武汉 430070
  • 收稿日期:2012-03-02 接受日期:2012-07-16 出版日期:2012-11-20 发布日期:2013-01-04
  • 通讯作者: 朱振东
  • 作者简介:* E-mail: zhuzd115@caas.net.cn
  • 基金资助:
    现代农业产业技术体系建设专项资金(CARS-09);作物种质资源保护项目(NB2010-2130135-25-14)

Genetic diversity in Fusarium solani f. sp. pisi based on SSR markers

Ni Xiang1,2, Yannong Xiao2, Canxing Duan1, Xiaoming Wang1, Zhendong Zhu1,*()   

  1. 1 Institute of Crop Science, Chinese Academy of Agricultural Sciences/The National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081
    2 College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070
  • Received:2012-03-02 Accepted:2012-07-16 Online:2012-11-20 Published:2013-01-04
  • Contact: Zhendong Zhu

摘要:

由茄镰孢豌豆专化型(Fusarium solani f. sp. pisi, Fsp)引起的根腐病是豌豆(Pisum sativum)最重要的病害之一。研究不同地理来源Fsp的遗传多样性, 对了解该菌的遗传背景及防治病害具有重要意义。本研究从血红丛赤壳交配群VI(Nectria haematococca MPVI)全基因组序列中筛选SSR位点, 选取107个SSR位点设计引物, 获得24对多态性引物。用24对多态性引物对不同地理来源的96个Fsp分离物进行遗传多样性分析, 结果表明, 24对引物共扩增出132个等位基因, 变异范围为3-15, 平均为5.5。基因多样性指数范围为0.4855-0.8264, 平均为0.7038。供试的96个Fsp分离物可分为93个基因型。聚类分析表明, 在相似性系数为0.8时, 96个Fsp分离物被划分为10个组群。Fsp分离物的地理来源或致病性与SSR聚类结果无关。分子方差分析(AMOVA)结果表明, Fsp遗传变异主要存在于群体内, 地理条件和生态区环境对Fsp遗传分化有显著影响。

关键词: 豌豆根腐病, 豌豆, Fusarium solani f. sp. pisi, SSR标记, 遗传变异

Abstract

Pea root rot, caused by Fusarium solani f. sp. pisi (Fsp), is one of the most important diseases on pea (Pisum sativum). Assessing the genetic diversity of the pathogen isolates from different geographical regions is crucially important for understanding of the genetic background of this pathogen and intelligently deploying host resistance. We screened SSRs in complete genome sequence of Nectria haematococca MPVI, and 107 SSR loci were selected for designing markers, from which 24 polymorphic primer pairs were developed. The 24 primer pairs were used to assess genetic diversity of 96 Fsp isolates from different geographical regions. Among 24 SSR markers, a total of 132 alleles were detected among the 96 Fsp isolates, the number of alleles for each of the loci ranged from 3 to 15 with an average of 5.5. The genetic diversity was estimated to range from 0.4855 to 0.8264 with the average value of 0.738. Using these markers, 93 genotypes were detected. When the genetic similarity coefficient was 0.8, 96 Fsp isolates were clustered into 10 groups by phylogenetic analysis. There was no correlation between SSR profile and either geographic origin or pathogenicity. Analysis of AMOVA revealed that variation mainly presented within Fsp populations (86.14%), and genetic differentiation of Fsp was significantly affected by geographical conditions and ecological environment.

Key words: pea root rot, Pisum sativum, Fusarium solani f. sp. pisi, SSR marker, genetic variation