生物多样性 ›› 2009, Vol. 17 ›› Issue (1): 82-87.DOI: 10.3724/SP.J.1003.2009.08266

• 研究简报 • 上一篇    下一篇

基于微卫星标记的5个尼罗罗非鱼品系的遗传多样性分析

梁宏伟1,2, 李忠1,2, 罗相忠1,2, 王长忠1,3, 呼光富1,3, 邹桂伟1,2(), 杨永铨4   

  1. 1 中国水产科学研究院长江水产研究所农业部淡水生物多样性保护与利用重点开放实验室, 荆州 434000
    2 中国水产科学研究院淡水渔业研究中心, 无锡 214081
    3 华中农业大学水产学院, 武汉 430070
    4 厦门鹭业水产有限公司, 厦门 361000
  • 收稿日期:2008-10-20 接受日期:2009-01-12 出版日期:2009-01-20 发布日期:2009-01-20
  • 通讯作者: 邹桂伟
  • 作者简介:* E-mail: zougw@yfi.ac.cn
  • 基金资助:
    国家科技基础条件平台专项(2006DKA30470-002)

Genetic diversity based on microsatellite markers in five Nile tilapia strains

Hongwei Liang1,2, Zhong Li1,2, Xiangzhong Luo1,2, Changzhong Wang1,3, Guangfu Hu1,3, Guiwei Zou1,2(), Yongquan Yang4   

  1. 1 Key Laboratory of Freshwater Biodiversity Conservation and Utilization, Ministry of Agriculture, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Jingzhou 434000
    2 Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081
    3 College of Fisheries, Huazhong Agricultural University, Wuhan 430070
    4 Xiamen Luye Fisheries Co., Ltd., Xiamen 361000
  • Received:2008-10-20 Accepted:2009-01-12 Online:2009-01-20 Published:2009-01-20
  • Contact: Guiwei Zou

摘要:

为了对生产上应用的5个尼罗罗非鱼(Oreochromis niloticus)品系的遗传背景进行本底调查,以期为育种工作提供基础数据, 本文用19对微卫星引物对5个尼罗罗非鱼品系(苏丹品系、台湾品系、吉富品系、超雄品系Ⅰ和超雄品系Ⅱ)进行了遗传多样性分析,计算并统计了等位基因数、多态信息含量(PIC)、杂合度、遗传相似性系数、遗传距离等参数。结果表明19个微卫星位点在5个罗非鱼品系中共检测到113个等位基因,平均期望杂合度在0.578-0.692之间,平均多态信息含量在0.473-0.628之间。5个尼罗罗非鱼品系有较丰富的遗传多样性,而超雄品系Ⅰ的遗传多样性相对较为贫乏。

关键词: Oreochromis niloticus, 遗传育种, 遗传变异, 超雄品系

Abstract

To provide background on the genetics of cultured Nile tilapia (Oreochromis niloticus) strains and basic information for breeding, we surveyed five Nile tilapia strains (Sudan, Taiwan, GIFT, SupermaleⅠand Supermale Ⅱ) using 19 microsatellite loci to estimate the genetic variation. A total of 113 alleles were detected. Mean expected heterozygosity (He) ranged between 0.578 and 0.692, and mean polymorphism information content (PIC) ranged between 0.473 and 0.628, indicating that these five Nile tilapia strains were genetically diverse. Of the five strains, the supermaleⅠhad less genetic variation compared with other strains.

Key words: Oreochromis niloticus, genetic breeding, genetic variation, supermale strains