长江下游地区4个克氏原螯虾群体的遗传多样性分析

doi:10.3724/SP.J.1003.2009.09017

生物多样性 ›› 2009, Vol. 17 ›› Issue (5): 518-523. DOI: 10.3724/SP.J.1003.2009.09017

长江下游地区4个克氏原螯虾群体的遗传多样性分析

王长忠¹^,², 李忠¹, 梁宏伟¹, 呼光富¹^,², 吴勤超¹^,², 邹桂伟¹^,²^,^*(), 罗相忠¹

1 中国水产科学研究院长江水产研究所农业部淡水生物多样性保护与利用重点开放实验室, 湖北荆州 434000
2 华中农业大学水产学院, 武汉 430070

收稿日期:2009-01-14 接受日期:2009-06-11 出版日期:2009-09-20 发布日期:2009-09-20
通讯作者: 邹桂伟
作者简介:*E-mail: zougw@yfi.ac.cn
基金资助:
国家科技基础条件平台专项(2006DKA30470-002)

Genetic diversity in fourProcambarus clarkii populations in the lower reaches of the Yangtze River

Changzhong Wang¹^,², Zhong Li¹, Hongwei Liang¹, Guangfu Hu¹^,², Qinchao Wu¹^,², Guiwei Zou¹^,²^,^*(), Xiangzhong Luo¹

1 Key Laboratory of Freshwater Biodiversity Conservation and Utilization, Ministry of Agriculture, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Jingzhou, Hubei 434000
2 Fisheries College of Huazhong Agricultural University, Wuhan 430070

Received:2009-01-14 Accepted:2009-06-11 Online:2009-09-20 Published:2009-09-20
Contact: Guiwei Zou

摘要/Abstract

摘要：

为了对长江下游地区克氏原螯虾(Procambarus clarkii)群体的遗传多样性状况进行本底调查, 本文利用17对微卫星引物对南京(NJ)、盱眙(XY)、合肥(HF)、南昌(NC)4个克氏原螯虾地理群体进行了遗传多样性研究。结果表明, 4个地理群体的平均观测杂合度为0.4322-0.4826、平均期望杂合度为0.4024-0.6121、平均多态信息含量为0.3408-0.5624, 4个克氏原螯虾群体的遗传多样性处于中等水平, 其中南京群体遗传多样性最高, 合肥群体最低; 4个克氏原螯虾群体的基因流为1.3729-5.9161, 遗传分化指数为0.0405-0.1540, 群体间基因流水平较高, 群体间遗传分化程度较小。聚类结果显示4个群体可分为2支: 南京、南昌、盱眙三个群体聚为一支, 合肥群体独自成一支。以上结果将为克氏原螯虾遗传育种、资源保护和利用提供一定的理论依据。

关键词: Procambarus clarkii, 微卫星, 遗传育种, 遗传变异

Abstract

To estimate genetic variation and provide genetic information for resource protection and breeding efforts, individuals of the invasive red swamp crayfish (Procambarus clarkii) from four populations along the lower reaches of the Yangtze River (Nanjing, Xuyi, Hefei and Nanchang) were characterized using 17 microsatellite loci. In these populations, the mean observed heterozygosity (H_o), mean expected heterozygosity (H_e), and mean polymorphism information content (PIC) ranged from 0.4322 to 0.4826, from 0.4024 to 0.6121, and from 0.3408 to 0.5624, respectively, indicating a medium level of genetic diversity. The highest and lowest genetic diversity were found in the Nanjing population and Hefei population, respectively. Gene flow (N_m) ranged from 1.3729 to 5.9161, and population differentiation (F_st) ranged from 0.0405 to 0.1540. Cluster analysis using the UPGMA method showed that the Nanjing and Nanchang populations clustered first, and then clustered with the Xuyi population, and finally all groups together clustered with the Hefei population.

Key words: Procambarus clarkii, microsatellite, genetic breeding, genetic variation

王长忠, 李忠, 梁宏伟, 呼光富, 吴勤超, 邹桂伟, 罗相忠 (2009) 长江下游地区4个克氏原螯虾群体的遗传多样性分析. 生物多样性, 17, 518-523. DOI: 10.3724/SP.J.1003.2009.09017.

Changzhong Wang, Zhong Li, Hongwei Liang, Guangfu Hu, Qinchao Wu, Guiwei Zou, Xiangzhong Luo (2009) Genetic diversity in fourProcambarus clarkii populations in the lower reaches of the Yangtze River. Biodiversity Science, 17, 518-523. DOI: 10.3724/SP.J.1003.2009.09017.

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链接本文: https://www.biodiversity-science.net/CN/10.3724/SP.J.1003.2009.09017

https://www.biodiversity-science.net/CN/Y2009/V17/I5/518

图/表 5

参考文献 12

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座位 Locus	引物序列(5′→3′) Primer sequence(5′→3′)	退火温度 Annealing temperature (℃)	重复类型 SSR motif	片段大小 Allele size (bp)	等位基因No. of alleles	登录号 GenBank accession no.
PclG02	F:CTCCCCATGCACTCTGGCTCTGT R:TGGCGAATTTTGCCTGTTTCTGTC	66	(GATA)₃GAGAA(GATA)₅	216-224	3	AF290919
PclG03	F:CTCTCCACCAGTCATTTCTT R:AAGCTTACAATAAATATAGATAGAC	52	(TCTA)₂₀	216-420	4	AF290920
PclG04	F:TATATCAGTCAATCTGTCCAG R:TCAGTAAGTAGATTGATAGAAGG	54	(TCTA)₃…(TCTA)₂…(TCTA)₂₉… (TCTA)₂	170-290	4	AF290921
PclG07	F:CCTCCCACCAGGGTTATCTATTCA R:GTGGGTGTGGCGCTCTTGTT	63	(TCTA)₈	100-160	4	AF290922
PclG08	F:ACGATAAATGGATAGATGGATGAA R:CCGGGTCTGTCTGTCTGTCA	62	(GATA)₁₆	148-220	5	AF290923
PclG09	F:TATGCACCTTTACCTGAAT R:TGTTGGTGTGGTCATCA	60	(TCTA)₁₄	80-160	4	AF290924
PclG10	F:TGCTCACGCAAACTTGTATTCAGT R:CAATGGTCCTTGATTTGGTGTTCT	54	(TAGA)₂TA(TAGA)₁₆	90-176	3	AF290925
PclG13	F:CTCTCCTGGCGCTGTTATTTAGC R:TGAAGAGGCAGAGTGAGGATTCTC	62	(TCTA)₁₂	130-150	2	AF290926
PclG15	F:GGCGTGACGCCAACGTGTCTT R:GGCTGGCCACTTTGTTAGCCTGAG	70	(TATC)₂TGTC(TATC)₁₇TATT(TATC)₃	150-185	3	AF290927
PclG16	F:CTCGGAATGTCCACCTGAGA R:TCATTATGGATTTTGTCAATCTAT	54	(TCTA)₁₈TCTC(TATC)₃	80-160	4	AF290928
PclG17	F:GTCGGGAACCTATTTACAGTGTAT R:AAGAGCGAAGAAAGAGATAAAGAT	57	(TCTA)₁₄	156-190	4	AF290929
PclG27	F:AATCTTAAGATCATGAAAAAGGTA R:TTTAAGGAACGTATAAGAAAAGAC	57	(TATC)₄CATC(TATC)₈	80-150	6	AF290932
PclG28	F:CTCGGCGAGTTTACTGAAAT R:AGAAGAAAGGGATATAAGGTAAAG	60	(GATA)₂₂(GA)₅	210-270	3	AF290933
PclG29	F:GAAAGTCATGGGTGTAGGTGTAAC R:TTTTTGGGCTATGTGACGAG	65	(TATC)₉	95-165	3	AF290934
PclG33	F:TTCGAGGCGTTGCTGATTGTAAGT R:CAAGGAAGCGTATAGCCGGAGTCT	68	(GT)₂₁	120-180	5	AF290936
PclG37	F:TAAATAAGTGGCGTGTAAGACGAG R:TAACTAAGCCAGGGTGGTCTCCAG	66	(CA)₄CG(CA)₁₅CG (CA)₂₃	80-180	7	AF290939
PclG48	F:CTGTTGGTGATTTCCGTCAATTTT R:AGATTCAACGCTGTGTTCCTGATC	66	(CA)₁₂	146-190	2	AF290941

座位 Locus	引物序列(5′→3′) Primer sequence(5′→3′)	退火温度 Annealing temperature (℃)	重复类型 SSR motif	片段大小 Allele size (bp)	等位基因No. of alleles	登录号 GenBank accession no.
PclG02	F:CTCCCCATGCACTCTGGCTCTGT R:TGGCGAATTTTGCCTGTTTCTGTC	66	(GATA)₃GAGAA(GATA)₅	216-224	3	AF290919
PclG03	F:CTCTCCACCAGTCATTTCTT R:AAGCTTACAATAAATATAGATAGAC	52	(TCTA)₂₀	216-420	4	AF290920
PclG04	F:TATATCAGTCAATCTGTCCAG R:TCAGTAAGTAGATTGATAGAAGG	54	(TCTA)₃…(TCTA)₂…(TCTA)₂₉… (TCTA)₂	170-290	4	AF290921
PclG07	F:CCTCCCACCAGGGTTATCTATTCA R:GTGGGTGTGGCGCTCTTGTT	63	(TCTA)₈	100-160	4	AF290922
PclG08	F:ACGATAAATGGATAGATGGATGAA R:CCGGGTCTGTCTGTCTGTCA	62	(GATA)₁₆	148-220	5	AF290923
PclG09	F:TATGCACCTTTACCTGAAT R:TGTTGGTGTGGTCATCA	60	(TCTA)₁₄	80-160	4	AF290924
PclG10	F:TGCTCACGCAAACTTGTATTCAGT R:CAATGGTCCTTGATTTGGTGTTCT	54	(TAGA)₂TA(TAGA)₁₆	90-176	3	AF290925
PclG13	F:CTCTCCTGGCGCTGTTATTTAGC R:TGAAGAGGCAGAGTGAGGATTCTC	62	(TCTA)₁₂	130-150	2	AF290926
PclG15	F:GGCGTGACGCCAACGTGTCTT R:GGCTGGCCACTTTGTTAGCCTGAG	70	(TATC)₂TGTC(TATC)₁₇TATT(TATC)₃	150-185	3	AF290927
PclG16	F:CTCGGAATGTCCACCTGAGA R:TCATTATGGATTTTGTCAATCTAT	54	(TCTA)₁₈TCTC(TATC)₃	80-160	4	AF290928
PclG17	F:GTCGGGAACCTATTTACAGTGTAT R:AAGAGCGAAGAAAGAGATAAAGAT	57	(TCTA)₁₄	156-190	4	AF290929
PclG27	F:AATCTTAAGATCATGAAAAAGGTA R:TTTAAGGAACGTATAAGAAAAGAC	57	(TATC)₄CATC(TATC)₈	80-150	6	AF290932
PclG28	F:CTCGGCGAGTTTACTGAAAT R:AGAAGAAAGGGATATAAGGTAAAG	60	(GATA)₂₂(GA)₅	210-270	3	AF290933
PclG29	F:GAAAGTCATGGGTGTAGGTGTAAC R:TTTTTGGGCTATGTGACGAG	65	(TATC)₉	95-165	3	AF290934
PclG33	F:TTCGAGGCGTTGCTGATTGTAAGT R:CAAGGAAGCGTATAGCCGGAGTCT	68	(GT)₂₁	120-180	5	AF290936
PclG37	F:TAAATAAGTGGCGTGTAAGACGAG R:TAACTAAGCCAGGGTGGTCTCCAG	66	(CA)₄CG(CA)₁₅CG (CA)₂₃	80-180	7	AF290939
PclG48	F:CTGTTGGTGATTTCCGTCAATTTT R:AGATTCAACGCTGTGTTCCTGATC	66	(CA)₁₂	146-190	2	AF290941

座位 Locus	南京群体 Nanjing population				盱眙群体 Xuyi population					合肥群体 Hefei population					南昌群体 Nanchang population
座位 Locus	PIC	观测杂合度 H_o	期望杂合度 H_e	平衡偏离指数 D		PIC	观测杂合度 H_o	期望杂合度 H_e	平衡偏离指数 D		PIC	观测杂合度 H_o	期望杂合度 H_e	平衡偏离指数 D		PIC	观测杂合度 H_o	期望杂合度 H_e	平衡偏离指数 D
PclG02	0.4446	0.4894	0.5067	-0.0341	0.4296		0.4783	0.5380	-0.1110	0.1693		0.1250	0.1886	-0.3372	0.5226		0.6250	0.6116	0.0219
PclG03	0.5127	0.4103	0.6051	-0.3219	0.5677		0.6222	0.6492	-0.0416	0.3705		0.5111	0.4966	0.0292	0.4087		0.5000	0.5290	-0.0548
PclG04	0.6663	0.3542	0.7265	-0.5125	0.3018		0.2292	0.3537	-0.3520	0.4868		0.5208	0.5524	-0.0572	0.3680		0.4583	0.4912	-0.0670
PclG07	0.5020	0.4894	0.5546	-0.1176	0.4536		0.3750	0.5570	-0.3268	0.4346		0.3750	0.4974	-0.2461	0.4039		0.4583	0.5068	-0.0957
PclG08	0.6980	0.3409	0.7531	-0.5473	0.5592		0.4583	0.6397	-0.2836	0.4093		0.5000	0.5267	-0.0507	0.4101		0.3542	0.4721	-0.2497
PclG09	0.6377	0.2500	0.7017	-0.6437	0.3775		0.1707	0.4279	-0.6011	0.4356		0.5814	0.5453	0.0662	0.5279		0.1591	0.6142	-0.7410
PclG10	0.4331	0.3864	0.5421	-0.2872	0.3664		0.0811	0.4498	-0.8197	0.0000		0.0000	0.0000	0.0000	0.2454		0.2045	0.2652	-0.2289
PclG13	0.3660	0.3542	0.4875	-0.2734	0.3225		0.3958	0.4086	-0.0313	0.0000		0.0000	0.0000	0.0000	0.3444		0.3617	0.4468	-0.1905
PclG15	0.6289	0.2292	0.2623	-0.1262	0.3263		0.4130	0.3641	0.1343	0.5916		0.7234	0.6728	0.0752	0.0976		0.1042	0.1011	0.0307
PclG16	0.6800	0.6170	0.7369	-0.1627	0.5934		0.5778	0.6504	-0.1116	0.4485		0.4643	0.5292	-0.1226	0.6629		0.6250	0.7230	-0.1356
PclG17	0.5867	0.4583	0.6607	-0.3063	0.4836		0.3750	0.5401	-0.3057	0.0000		0.0000	0.0000	0.0000	0.5642		0.6458	0.6465	-0.0011
PclG27	0.6902	0.5909	0.7450	-0.2069	0.6812		0.7391	0.7360	0.0042	0.3045		0.4255	0.3672	0.1588	0.6853		0.6809	0.7380	-0.0774
PclG28	0.5517	0.2045	0.6335	-0.6772	0.4092		0.1087	0.4909	-0.7786	0.0000		0.0000	0.0000	0.0000	0.3733		0.3750	0.5018	-0.2527
PclG29	0.4904	0.3830	0.5726	-0.3311	0.4366		0.6522	0.5162	0.2635	0.5558		0.5870	0.6362	-0.0773	0.3884		0.3043	0.5115	-0.4051
PclG33	0.5800	1.0000	0.6536	0.5300	0.6700		1.0000	0.7550	0.3245	0.5862		1.0000	0.6577	0.5205	0.7290		1.0000	0.7746	0.2910
PclG37	0.7231	1.0000	0.7703	0.2982	0.6837		1.0000	0.7389	0.3534	0.6470		1.0000	0.7076	0.4132	0.5673		1.0000	0.6436	0.5538
PclG48	0.3686	0.4318	0.4929	-0.1240	0.2490		0.3125	0.2945	0.0611	0.3533		0.5333	0.4634	0.1508	0.3629		0.3478	0.4816	-0.2778

座位 Locus	南京群体 Nanjing population				盱眙群体 Xuyi population					合肥群体 Hefei population					南昌群体 Nanchang population
座位 Locus	PIC	观测杂合度 H_o	期望杂合度 H_e	平衡偏离指数 D		PIC	观测杂合度 H_o	期望杂合度 H_e	平衡偏离指数 D		PIC	观测杂合度 H_o	期望杂合度 H_e	平衡偏离指数 D		PIC	观测杂合度 H_o	期望杂合度 H_e	平衡偏离指数 D
PclG02	0.4446	0.4894	0.5067	-0.0341	0.4296		0.4783	0.5380	-0.1110	0.1693		0.1250	0.1886	-0.3372	0.5226		0.6250	0.6116	0.0219
PclG03	0.5127	0.4103	0.6051	-0.3219	0.5677		0.6222	0.6492	-0.0416	0.3705		0.5111	0.4966	0.0292	0.4087		0.5000	0.5290	-0.0548
PclG04	0.6663	0.3542	0.7265	-0.5125	0.3018		0.2292	0.3537	-0.3520	0.4868		0.5208	0.5524	-0.0572	0.3680		0.4583	0.4912	-0.0670
PclG07	0.5020	0.4894	0.5546	-0.1176	0.4536		0.3750	0.5570	-0.3268	0.4346		0.3750	0.4974	-0.2461	0.4039		0.4583	0.5068	-0.0957
PclG08	0.6980	0.3409	0.7531	-0.5473	0.5592		0.4583	0.6397	-0.2836	0.4093		0.5000	0.5267	-0.0507	0.4101		0.3542	0.4721	-0.2497
PclG09	0.6377	0.2500	0.7017	-0.6437	0.3775		0.1707	0.4279	-0.6011	0.4356		0.5814	0.5453	0.0662	0.5279		0.1591	0.6142	-0.7410
PclG10	0.4331	0.3864	0.5421	-0.2872	0.3664		0.0811	0.4498	-0.8197	0.0000		0.0000	0.0000	0.0000	0.2454		0.2045	0.2652	-0.2289
PclG13	0.3660	0.3542	0.4875	-0.2734	0.3225		0.3958	0.4086	-0.0313	0.0000		0.0000	0.0000	0.0000	0.3444		0.3617	0.4468	-0.1905
PclG15	0.6289	0.2292	0.2623	-0.1262	0.3263		0.4130	0.3641	0.1343	0.5916		0.7234	0.6728	0.0752	0.0976		0.1042	0.1011	0.0307
PclG16	0.6800	0.6170	0.7369	-0.1627	0.5934		0.5778	0.6504	-0.1116	0.4485		0.4643	0.5292	-0.1226	0.6629		0.6250	0.7230	-0.1356
PclG17	0.5867	0.4583	0.6607	-0.3063	0.4836		0.3750	0.5401	-0.3057	0.0000		0.0000	0.0000	0.0000	0.5642		0.6458	0.6465	-0.0011
PclG27	0.6902	0.5909	0.7450	-0.2069	0.6812		0.7391	0.7360	0.0042	0.3045		0.4255	0.3672	0.1588	0.6853		0.6809	0.7380	-0.0774
PclG28	0.5517	0.2045	0.6335	-0.6772	0.4092		0.1087	0.4909	-0.7786	0.0000		0.0000	0.0000	0.0000	0.3733		0.3750	0.5018	-0.2527
PclG29	0.4904	0.3830	0.5726	-0.3311	0.4366		0.6522	0.5162	0.2635	0.5558		0.5870	0.6362	-0.0773	0.3884		0.3043	0.5115	-0.4051
PclG33	0.5800	1.0000	0.6536	0.5300	0.6700		1.0000	0.7550	0.3245	0.5862		1.0000	0.6577	0.5205	0.7290		1.0000	0.7746	0.2910
PclG37	0.7231	1.0000	0.7703	0.2982	0.6837		1.0000	0.7389	0.3534	0.6470		1.0000	0.7076	0.4132	0.5673		1.0000	0.6436	0.5538
PclG48	0.3686	0.4318	0.4929	-0.1240	0.2490		0.3125	0.2945	0.0611	0.3533		0.5333	0.4634	0.1508	0.3629		0.3478	0.4816	-0.2778

群体 Population	代号 Code	平均等位基因数 Mean N_a	平均有效等位基因数 Mean N_e	平均多态信息含量 Mean PIC	平均观测杂合度 Mean H_o	平均期望杂合度 Mean H_e
南京群体 Nanjing population	NJ	3.7647	2.7778	0.5624	0.4700	0.6121
盱眙群体 Xuyi population	XY	3.4706	2.3455	0.4654	0.4699	0.5359
合肥群体 Hefei population	HF	2.5294	1.9415	0.3408	0.4322	0.4024
南昌群体 Nanchang population	NC	3.2941	2.3656	0.4507	0.4826	0.5329
平均 Mean		3.2647	3.3576	0.4548	0.4637	0.5208

长江下游地区4个克氏原螯虾群体的遗传多样性分析

Genetic diversity in fourProcambarus clarkii populations in the lower reaches of the Yangtze River

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群体 Population	南京 Nanjing	盱眙 Xuyi	合肥Hefei	南昌Nanchang
南京 Nanjing	****	0.8475	0.7871	0.8997
盱眙 Xuyi	0.1655	****	0.6898	0.8309
合肥 Hefei	0.2394	0.3714	****	0.8239
南昌 Nanchang	0.1057	0.1852	0.1937	****

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