生物多样性 ›› 2006, Vol. 14 ›› Issue (6): 498-507.DOI: 10.1360/biodiv.060055

• 论文 • 上一篇    下一篇

采用微卫星标记分析13个中外牛品种的遗传变异和品种间的遗传关系

罗永发1, 王志刚2, 李加琪1, 张桂香2*, 陈瑶生3, 梁勇1, 于福清2, 宋卫涛4, 张自富5   

  1. 1 (华南农业大学动物科学学院遗传育种实验室, 广州 510642)
    2 (农业部全国畜牧兽医总站畜禽牧草种质资源保存利用中心, 北京 100094)
    3 (中山大学生命科学学院, 广州 510275)
    4 (扬州大学动物科学与技术学院, 扬州 225009)
    5 (西北农林科技大学动物科技学院, 陕西杨凌 712100 )
  • 收稿日期:2006-03-17 修回日期:2006-08-29 出版日期:2006-11-20 发布日期:2006-11-20
  • 通讯作者: 张桂香

Genetic variation and genetic relationship among 13 Chinese and intro-duced cattle breeds using microsatellite DNA markers

Yongfa Luo 1, Zhigang Wang 2, Jiaqi Li 1, Guixiang Zhang 2*, Yaosheng Chen 3, Yong Liang 1, Fuqing Yu 2, Weitao Song 4, Zifu Zhang 5   

  1. 1 Department of Animal Science, South China Agricultural University, Guangzhou 510642
    2 Center of Preservation and Utilization of Genetic Resources of Domestic Animals and Forage, National Animal Hus-bandry and Veterinary Service, Beijing 100094
    3 College of Life Sciences, Sun Yat-Sen University, Guangzhou 510275
    4 College of Animal Science and Technology , Yangzhou University, Yangzhou 225009
    5 College of Animal Science and Technology, Northwest Sci-Tech University of Agriculture & Forestry, Yangling, Shaanxi 712100
  • Received:2006-03-17 Revised:2006-08-29 Online:2006-11-20 Published:2006-11-20
  • Contact: Guixiang Zhang

摘要: 本研究应用联合国粮农组织(FAO)和国际动物遗传学会(ISAG)推荐的10对微卫星引物, 结合荧光–多重PCR技术, 检测了10个中国地方黄牛品种和3个外来牛品种的基因型。通过计算基因频率、多态信息含量和遗传杂合度,以Nei’s遗传距离和Nei’s标准遗传距离为基础, 采用非加权组对算术平均聚类法构建了聚类图, 分析了13个牛品种的群体内遗传变异和群体间遗传关系。并以聚类分析和群体结构分析为基础, 将13个中外黄牛品种分为三类: Ⅰ类属于普通黄牛品种, 包括延边牛、沿江牛、长白地方牛、蒙古牛、阿勒泰白头牛、哈萨克牛、复州牛和西藏牛; Ⅱ类属于含有瘤牛血统的黄牛品种, 包括日喀则驼峰牛和阿沛甲咂牛; Ⅲ类属于外来牛品种, 包括德国黄牛、西门塔尔牛和夏洛来牛。研究结果为加强我国地方黄牛品种种质特性研究以及地方牛品种资源的保护与利用提供了科学的依据。

AbstractThe genotypes of 10 indigenous Chinese cattle breeds and three introduced breeds were investigated using 10 microsatellite DNA markers recommended by the Food and Agriculture Organization of the United Nations (FAO) and the International Society of Animal Genetics (ISAG) through fluorescence-multiplex PCR. The allele frequency, polymorphism information content (PIC), and heterozygosity were computed to estimate the genetic variation of each population. To determine the genetic relationships among the breeds, phylogenetic trees were constructed based on Nei’s genetic distance and Nei’s standard genetic distance using the unweighted pair-group method with arithmetic mean. As a result, 13 breeds were clustered into three groups. The first group belongs to the common cattle breeds including Yanbian, Changbai, Yanjiang, Monggol, Altay White Head, Hazake, Fuzhou, and Tibetan, while the second group contains those with zebu blood including Xigazê Humped and Apeijiaza. The third group consists of foreign breeds including German Yellow, Simmental, and Charolai. The results may provide a scientific basis for the study of characteristics of Chinese indigenous cattle breeds so as to preserve and utilize them.