荒漠生境油蒿根围AM真菌多样性

doi:10.3724/SP.J.1003.2009.09020

摘要/Abstract

摘要：

为了阐明荒漠生境主要植被油蒿(Artemisia ordosica)根围AM真菌多样性, 2007年8月从毛乌素沙地和腾格里沙漠选取榆林、盐池、研究站和沙坡头4个样地, 按0-10、10-20、20-30、30-40、40-50 cm 5个土层采集油蒿根围土壤样品, 研究了油蒿根围AM真菌物种多样性和生态分布。在分离的4属28种AM真菌中, 球囊霉属(Glomus)16种, 无梗囊霉属(Acaulospora)7种, 盾巨孢囊霉属(Scutellospora)4种, 多孢囊霉属(Diversispora)1种。4个样地的共同优势种为摩西球囊霉(G. mosseae), 共同常见种为双网无梗囊霉(A. bireticulata)和网状球囊霉(G. reticulatum), 共同稀有种为缩球囊霉(G. constrictum)。地球囊霉(G. geosporum)仅出现在盐池, 蜜色无梗囊霉(A. mellea)、帚状球囊霉(G. coremioides)、浅窝无梗囊霉(A. lacunosa)和宽柄球囊霉(G. magnicaule)仅出现在研究站, 黑球囊霉(G. melanosporum)仅出现在榆林。盐池与研究站样地AM真菌种类最多, 榆林样地孢子密度最大, 沙坡头样地种类和孢子密度显著偏低。总体上, 孢子密度、分离频度、相对多度和重要值依Glomus > Acaulospora > Scutellospora>Diversispora呈现显著减小趋势。结果表明, 油蒿与AM真菌之间有良好共生性, 这对进一步利用菌根生物技术维护荒漠生态系统结构的完整性具有重要意义。

关键词: Artemisia ordosica, 毛乌素沙地, 腾格里沙漠, 共生, 孢子密度, 相对多度, 重要值

Abstract

To elucidate the diversity of arbuscular mycorrhizal (AM) fungi associated with the common desert plant Artemisia ordosica, we sampled, in April 2007, the following four representative sites in the Mu Us Sandland and Tengger Desert regions: Yulin Psammophyte Garden (Yulin), Yanchi Desert Shrub Garden (Yanchi), Ordos Sandland Ecological Research Station (Research Station), and Shapotou Desert Research and Experiment Station (Shapotou). We collected soil samples from the rhizosphere ofA. ordosica and divided them into five depth intervals, each 10 cm, to 50 cm depth. A total of 28 AM fungal species from four genera were isolated. Of these, 16 species belonged to Glomus, 7 to Acaulospora, 4 to Scutellospora and 1 toDiversispora. Glomus mosseae was the dominant species. Acaulospora bireticulata andG. reticulatum were common species (>25% frequency of occurence) andG. constrictum was rare species (≤25% frequency of occurence) at the four sampling sites.Glomus geosporum was found only in Yanchi, G. melanosporum only in Yulin and A. mellea, A. lacunosa, G. coremioides and G. magnicaule occurred only in Research Station. Richness of AM species was highest in Yanchi and Research Station and spore density was highest in Yulin. Species richness and spore density were the lowest in Shapotou. Generally, spore density, isolation frequency, relative abundance and importance value followed the trend Glomus > Acaulospora > Paraglomus > Diversispora. Our results are the first to assess the considerable diversity of arbuscular mycorrhizal fungi found in association with Artemisia ordosica. Further research should concentrate on understanding the dynamics of these relationships.

Key words: Artemisia ordosica, Mu Us Sandland, Tengger Desert, spore density, importance value

钱伟华, 贺学礼 (2009) 荒漠生境油蒿根围AM真菌多样性. 生物多样性, 17, 506-511. DOI: 10.3724/SP.J.1003.2009.09020.

Weihua Qian, Xueli He (2009) Diversity of arbuscular mycorrhizal fungi associated with a desert plant Artemisia ordosica. Biodiversity Science, 17, 506-511. DOI: 10.3724/SP.J.1003.2009.09020.

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https://www.biodiversity-science.net/CN/Y2009/V17/I5/506

图/表 5

参考文献 23

[1]	Bashan Y, Davis EA, Carrillo-Garcia A, Linderman RG (2000) Assessment of VA mycorrhizal inoculum potential in relation to the establishment of cactus seedlings under mesquite nurse-trees in the Sonoran Desert. Applied Soil Ecology, 14,165-175.
[2]	Bethlenfalvay GJ (1992) Vesicular-arbuscular mycorrhizal fungi in nitrogen-fixing legumes:problems and prospects methods. In: Methods in Microbiology: Techniques for the Study of Mycorrhiza (eds Norris JR, Read DJ, Varma AK), pp.376-389. Academic Press, London.
[3]	Bever JD, Schuhz PA, Pringle A, Morton JB (2001) Arbuscular mycorrhizal fungi: more diverse than meets the eye, and the ecological tale of why. BioScience, 51,923-931.
[4]	Bonfante-Fasolo P (1984) Anatomy and morphology of VA mycorrhiza. In: VA Mycorrhizae (eds Powell CL, Bagyaraj DJ), pp.5-33. CRC Press, Boca Raton.
[5]	Brundrett MC (1991) Mycorrhizas in natural ecosystems. In: Advances in Ecological Research (eds Macfayden A, Begon M, Fitter AH), pp.171-313. Academic Press, London.
[6]	Chen N (陈宁), Wang YS (王幼珊), Yang YJ (杨延杰), Lin D (林多), Qiu HW (仇宏伟), Ni XH (倪小会), Zhang MQ (张美庆) (2006) Effects of host plants on development of arbuscular mycorrhizal fungi. Acta Agriculturae Boreali- Sinica (华北农学报), 21(6),103-106. (in Chinese with English abstract)
[7]	Daniels BA, Skipper HD (1982) Methods for the recovery and quantitative estimation of propagules from soil. In: Methods and Principles of Mycorrhizal Research (ed. Schenck NC), pp.24-35. The American Phytopathological Society, St. Paul.
[8]	Ferrol N, Calvente R, Cano C (2004) Analysing arbuscular mycorrhizal fungal diversity in shrub-associated resource islands from a desertification threatened semiarid Mediterranean ecosystem. Applied Soil Ecology, 25,123-133.
[9]	He XL (贺学礼), Hou XF (侯晓飞) (2008) Spatio-temporal distribution of arbuscular mycorrhizal fungi from Artemisia ordosica in Yulin City of Shaanxi Province, China. Journal of Plant Ecology (Chinese Version)(植物生态学报), 32,1373-1377. (in Chinese with English abstract)
[10]	He XL (贺学礼), Li B (李斌) (1999) Selection research of VA mycorrhizal fungus and plant. Acta Botanica Boreali- Occidentalia Sinica (西北植物学报), 19,471-475. (in Chinese with English abstract)
[11]	Jasper DA, Abbot LK, Robson AD (1991) The effect of soil disturbance on vesicular-arbuscular mycorrhizal fungi in soils from different vegetation types. New Phytologist, 118,471-476.
[12]	Kennedy AC, Smith KL (1995) Soil microbial diversity and the sustainability of agriculture soils. Plant and Soil, 170,75-86.
[13]	Kennedy LJ, Tiller RL, Stutz JC (2002) Associations between arbuscular mycorrhizal fungi and Sporobolus wrightii in riparian habitats in arid south-western North America. Journal of Arid Environments, 50,459-475.
[14]	Koske RE (1987) Distribution of VA mycorrhizal fungi along a latitudinal temperature gradient. Mycologia, 79,55-68.
[15]	Liu RJ (刘润进), Liu PQ (刘鹏起), Xu K (徐坤), Lü ZF (吕志范) (1999) Ecological distribution of arbuscular mycorrhizal fungi in saline-alkaline soils of China. Chinese Journal of Applied Ecology (应用生态学报), 10,721-724. (in Chinese with English abstract)
[16]	Lu RK (鲁如坤) (1999) Methods for Agricultural Chemical Analysis of Soils (土壤农业化学分析方法). China Agricultural Press, Beijing. (in Chinese)
[17]	Morton JB, Redecker D (2001) Two new families of Glomales, Archaeosporaceae and Paraglomaceae, with two new genera Archaeospora and Paraglomus, based on concordant molecular and morphological characters. Mycologia, 93,181-195.
[18]	Peter DS, Williams SE, Martha C (1988) Efficacy of native vesicular-arbuscular mycorrhizal fungi after severe soil disturbance. New Phytologist, 110,347-354.
[19]	Schenck NC, Perez Y (1988) Manual for Identification of Vesicular Arbuscular Mycorrhizal Fungi, 2nd edn. INVAM University of Florida Gainesville, Florida, USA.
[20]	Shi ZY (石兆勇), Zhang LY (张立运), Feng G (冯固), Peter C, Tian CY (田长彦), Li XL (李晓林) (2006) AM fungi diversity of ephemeral under and outside Tamarix shrub. Chinese Science Bulletin (科学通报), 51(Suppl.),108-114. (in Chinese)
[21]	Smith FA, Smith SE (1997) Structural diversity in (vesicular)- arbuscular mycorrhizal symbioses. New Phytologist, 137,373-388.
[22]	Zhang MQ (张美庆), Wang YS (王幼珊), Xing LJ (邢礼军) (1999) The relationship between the distribution of AM fungi and environmental factors. Mycosystema (菌物系统), 18,25-29. (in Chinese with English abstract)
[23]	Zhou ZJ (周自江), Zhang GC (章国材) (2003) Typical severe dust storms in North China from 1954 to 2002. Chinese Science Bulletin (科学通报), 48,1224-1228. (in Chinese)

样地 Site	地点 Locality	速效磷 Available P (μg/g)	速效氮 Available N (mg/g)	有机质 Organic matter (μg/g)	pH	孢子密度 Spore density (inds./20 g)	种丰度 Species richness
榆林 Yulin	陕西榆林沙生植物园 Yulin Psammophyte Garden, Shaanxi	2.639	5.04	2.304	7.59	174.25	16
盐池 Yanchi	宁夏盐池县沙生灌木园 Yanchi Desert Shrub Garden, Ningxia	3.591	9.8	5.794	9.35	159.5	23
研究站 Research Station	鄂尔多斯沙地草地生态研究站 Ordos Sandland Ecological Research Station, CAS	2.896	13.23	3.77	8.87	111.5	25
沙坡头 Shapotou	中国科学院沙坡头沙漠试验研究站 Shapotou Desert Research and Experiment Station, CAS	1.657	9.45	3.457	8.96	63	8

样地 Site	地点 Locality	速效磷 Available P (μg/g)	速效氮 Available N (mg/g)	有机质 Organic matter (μg/g)	pH	孢子密度 Spore density (inds./20 g)	种丰度 Species richness
榆林 Yulin	陕西榆林沙生植物园 Yulin Psammophyte Garden, Shaanxi	2.639	5.04	2.304	7.59	174.25	16
盐池 Yanchi	宁夏盐池县沙生灌木园 Yanchi Desert Shrub Garden, Ningxia	3.591	9.8	5.794	9.35	159.5	23
研究站 Research Station	鄂尔多斯沙地草地生态研究站 Ordos Sandland Ecological Research Station, CAS	2.896	13.23	3.77	8.87	111.5	25
沙坡头 Shapotou	中国科学院沙坡头沙漠试验研究站 Shapotou Desert Research and Experiment Station, CAS	1.657	9.45	3.457	8.96	63	8

样地 Site	AM真菌属 Genera	孢子密度 Spore density (inds./20 g soil)	分离频度 Isolation frequency (%)	相对多度 Relative abundance (%)	重要值 Importance value
榆林 Yulin	无梗囊霉属 Acaulospora	36.00^b	75^b	20.50^b	47.75^b
	球囊霉属 Glomus	136.75^a	100^a	78.50^a	89.25^a
	盾巨孢囊霉属 Scutellospora	1.50^c	50^c	1.00^c	25.50^c
盐池 Yanchi	无梗囊霉属 Acaulospora	30.75^b	75^b	19.00^b	47.00^b
	球囊霉属 Glomus	110.50^a	100^a	69.25^a	84.63^a
	盾巨孢囊霉属 Scutellospora	118.25^c	75^b	11.50^c	43.25^c
研究站 Research Station	无梗囊霉属 Acaulospora	22.50^b	100^a	19.75^b	59.87^b
	球囊霉属 Glomus	79.75^a	100^a	71.50^a	85.75^a
	盾巨孢囊霉属 Scutellospora	9.50^c	75^b	8.50^c	41.75^c
	多孢囊霉属 Diversispora	0.25^d	25^c	0.25^d	12.63^d
沙坡头 Shapotou	无梗囊霉属 Acaulospora	18.00^b	50^b	15.05^b	32.75^b
沙坡头 Shapotou	球囊霉属 Glomus	61.00^a	100^a	84.59^a	92.30^a

样地 Site	AM真菌属 Genera	孢子密度 Spore density (inds./20 g soil)	分离频度 Isolation frequency (%)	相对多度 Relative abundance (%)	重要值 Importance value
榆林 Yulin	无梗囊霉属 Acaulospora	36.00^b	75^b	20.50^b	47.75^b
	球囊霉属 Glomus	136.75^a	100^a	78.50^a	89.25^a
	盾巨孢囊霉属 Scutellospora	1.50^c	50^c	1.00^c	25.50^c
盐池 Yanchi	无梗囊霉属 Acaulospora	30.75^b	75^b	19.00^b	47.00^b
	球囊霉属 Glomus	110.50^a	100^a	69.25^a	84.63^a
	盾巨孢囊霉属 Scutellospora	118.25^c	75^b	11.50^c	43.25^c
研究站 Research Station	无梗囊霉属 Acaulospora	22.50^b	100^a	19.75^b	59.87^b
	球囊霉属 Glomus	79.75^a	100^a	71.50^a	85.75^a
	盾巨孢囊霉属 Scutellospora	9.50^c	75^b	8.50^c	41.75^c
	多孢囊霉属 Diversispora	0.25^d	25^c	0.25^d	12.63^d
沙坡头 Shapotou	无梗囊霉属 Acaulospora	18.00^b	50^b	15.05^b	32.75^b
沙坡头 Shapotou	球囊霉属 Glomus	61.00^a	100^a	84.59^a	92.30^a

种 Species	孢子密度 Spore density (inds./20 g soil)				分离频度 Isolation frequency (%)
	榆林 Yulin	盐池 Yanchi	研究站 Research Station	沙坡头 Shapotou	榆林Yulin	盐池 Yanchi	研究站 Research Station	沙坡头 Shapotou
	凹坑无梗囊霉 Acaulospora excauata	0^b	1.75^ab	2.25^a	0^b	0	50	75	0
双网无梗囊霉 A. bireticulata	6.25^a	10.25^a	10.50^a	8.25^a	75	75	75	75
沙荒球囊霉 Glomus deserticola	3.25^ab	1.25^b	7.00^a	0^b	50	50	75	0
网状球囊霉 G. reticulatum	11.25^a	12.00^a	9.25^a	12.50^a	75	75	75	75
多梗球囊霉 G. multicaule	0.50^b	0.50^b	0.25^b	2.25^a	25	25	25	75
集球囊霉 G. fasciculatum	9.50^a	3.75^ab	0^b	0.50^b	75	50	0	25