祖先性状重建法揭示铁线蕨属植物孢子表面纹饰的形态多样性及其演化

doi:10.17520/biods.2019111

生物多样性 ›› 2019, Vol. 27 ›› Issue (11): 1228-1235.DOI: 10.17520/biods.2019111

祖先性状重建法揭示铁线蕨属植物孢子表面纹饰的形态多样性及其演化

赵国华^1,²,王莹²,商辉²,周喜乐³,王爱华⁴,李玉峰⁵,王晖¹,刘保东⁶,严岳鸿^2,^*()

1 深圳市中国科学院仙湖植物园, 广东深圳 518004
2 上海辰山植物园, 中国科学院上海辰山植物科学研究中心, 上海 201602
3 湘西土家族苗族自治州森林资源监测中心, 湖南吉首 416000
4 南宁师范大学北部湾环境演变与资源利用教育部重点实验室, 南宁 530001
5 湖南科技大学生命科学学院, 湖南湘潭 411201
6 哈尔滨师范大学植物生物学黑龙江省高校重点实验室, 哈尔滨 150025

收稿日期:2019-03-29 接受日期:2019-10-08 出版日期:2019-11-20 发布日期:2020-01-17
通讯作者: 严岳鸿
基金资助:
国家标本资源共享平台项目、科技部科技基础性工作专项(2013FY112100);生物多样性调查与评估项目(2019HJ2096001006);上海市绿化与市容管理局科学技术项目(G162416);湖南省教育厅科学研究项目(10C0720)

Ancestral state reconstruction reveals the diversity and evolution of spore ornamentation in Adiantum (Pteridaceae)

Guohua Zhao^1,²,Ying Wang²,Hui Shang²,Xile Zhou³,Aihua Wang⁴,Yufeng Li⁵,Hui Wang¹,Baodong Liu⁶,Yuehong Yan^2,^*()

1 Fairylake Botanical Garden, Shenzhen & Chinese Academy of Sciences, Shenzhen, Guangdong 518004;
2 Shanghai Chenshan Botanical Garden, Shanghai Chenshan Plant Science Research Center, Chinese Academy of Sciences, Shanghai 201602
3 Xiangxi Tujia and Miao Autonomous Prefecture Forest Resources Monitoring Center, Jishou, Hunan 416000
4 Key Laboratory of Beibu Gulf Environment Change and Resources Use of Ministry of Education, Nanning Normal University, Nanning 530001
5 School of Life Science, Hunan University of Science and Technology, Xiangtan, Hunan 411201
6 Harbin Normal University, Key Laboratory of Plant Biology, College of Heilongjiang Province, Harbin 150025

Received:2019-03-29 Accepted:2019-10-08 Online:2019-11-20 Published:2020-01-17
Contact: Yan Yuehong

1. 附录1 GenBank下载的参比材料(rbcL, atpB, atpA, trnL-F和rps4-trnS).pdf(279KB)

摘要/Abstract

摘要：

蕨类植物孢子表面的纹饰形态复杂多样, 在分类学和古生物学研究中具有重要意义, 目前探索祖先性状演化常用形态学与系统发育学结合的方法。本文选取一回羽叶铁线蕨类植物为材料, 用扫描电子显微镜观察其孢子形态, 用5个叶绿体基因序列构建系统树, 用最大似然法和最大简约法来分析孢子性状的演化。结果表明: 一回羽叶铁线蕨类的孢子纹饰有5种类型, 即光滑、粗糙、颗粒、疣状及瘤状; 这一类群的祖先孢子纹饰有很大的可能是瘤状纹饰; 疣状纹饰可能是由瘤状纹饰演化而来; 孢子纹饰由简单向复杂演化, 越进化的物种其孢子纹饰越复杂。

关键词: 蕨类, 孢子纹饰, 系统发育, 性状演化

Abstract:

Spore morphology is diverse and complex in ferns, thus serving as an important indicator of taxonomy. In conjunction, morphological traits and phylogeny are used to study taxonomy and recent character evolution. This study analyzes the evolution of spore ornamentation in once-pinnate maidenhair ferns (Adiantum) using scanning electron microscopy and ancestral state reconstruction. Our results show that there are five ornamental types in once-pinnate maidenhair ferns: psilate, scabrate, granulate, verrucate and tuberculate. The ancestral state of spore ornamentation in Adiantum is probably tuberculate, with verrucate ornamentation possibly deriving from tuberculate. We suggest that spore ornamentation evolved from simple to complex in Adiantum. In other words, the more evolutionary species, will with a more complex spore ornamentation.

Key words: fern, spore ornamentation, phylogenetic, character evolution

赵国华, 王莹, 商辉, 周喜乐, 王爱华, 李玉峰, 王晖, 刘保东, 严岳鸿. 祖先性状重建法揭示铁线蕨属植物孢子表面纹饰的形态多样性及其演化[J]. 生物多样性, 2019, 27(11): 1228-1235.

Guohua Zhao, Ying Wang, Hui Shang, Xile Zhou, Aihua Wang, Yufeng Li, Hui Wang, Baodong Liu, Yuehong Yan. Ancestral state reconstruction reveals the diversity and evolution of spore ornamentation in Adiantum (Pteridaceae)[J]. Biodiv Sci, 2019, 27(11): 1228-1235.

图/表 5

参考文献 34

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物种 Species	标本号及采集地 Vouchers and locality	赤道轴 × 极轴 Equatorial diam × polar diam (µm)	孢子纹饰 Spore ornamentation	图1中位置 Position in Fig. 1
A. latifolium	Zxl9882. 泰国曼谷植物标本馆 Bangkok Herbarium, Bangkok, Thailand	33.5 × 27.0	粗糙 Scabrate	1A
扇叶铁线蕨 A. flabellulatum	Zxl10018. 中国广东南岭国家森林公园 Nanling National Forest Park, Guangdong, China	45.5 × 31.2	颗粒 Granulate	1B
团羽铁线蕨 A. capillus-junonis	Zxl9959. 中国广东南岭国家森林公园 Nanling National Forest Park, Guangdong, China	46.5 × 43.0	光滑 Psilate	1C
半月形铁线蕨 A. philippense	Yan12405, Yan12086. 中国云南元江; Zxl9839. 泰国彭世洛府屯萨朗峦国家公园 Yan12405, Yan12086. Yuanjiang, Yunnan, China; Zxl9839. Thung Salaeng Luang National Park, Phitsanulok, Thailand	33.4 × 25.7	粗糙 Scabrate	1D
孟连铁线蕨 A. menglianense	Yan12415, Yan12409. 中国云南元江 Yuanjiang, Yunnan, China	31.5 × 26.6	颗粒 Granulate	1E
翅柄铁线蕨 A. soboliferum	Yan11393. 中国海南昌江 Changjiang, Hainan, China	34.0 × 30.5	颗粒 Granulate	1F
A. zollingeri	Zxl9812, Zxl9911, ZXL9851. 泰国碧武里府康卡沾国家公园 Kaeng Krachan National Park, Phetchaburi, Thailand	34.0 × 27.3	瘤状 Tuberculate	1G
鞭叶铁线蕨 A. caudatum	Yan12408. 中国云南元江 Yuanjiang, Yunnan, China	40.6 × 33.5	瘤状 Tuberculate	1H
白垩铁线蕨 A. gravesii	WYG393. 中国贵州贞丰 Zhenfeng, Guizhou, China	51.5 × 36.1	瘤状 Tuberculate	1I
小铁线蕨 A. mariesii	Zxl9685. 中国湖南桑植 Sangzhi, Hunan, China	36.8 × 30.1	疣状 Verrucate	1J
仙霞铁线蕨 A. juxtapositum	Yan1205071. 中国广东仁化 Renhua, Guangdong, China	60.4 × 45.7	疣状 Verrucate	1K
普通铁线蕨 A. edgewothii	Yan12412. 中国云南元江 Yuanjiang, Yunnan, China	30.0 × 25.6	瘤状 Tuberculate	1L
假鞭叶铁线蕨 A. malesianum	Yan12416, Yan12404. 中国云南元江 Yuanjiang, Yunnan, China	38.8 × 32.8	瘤状 Tuberculate	1M
梅山铁线蕨 A. × meishanianum	Yan12411. 中国云南元江 Yuanjiang, Yunnan, China	-	瘤状 Tuberculate	1N
苍山铁线蕨 A. sinicum	Yan112601, Yan12414. 中国云南元江 Yuanjiang, Yunnan, China	31.7 × 29.1	瘤状 Tuberculate	1O
哀牢山铁线蕨 A. × ailaoshanense	Yan12413, Yan12410. 中国云南元江 Yuanjiang, Yunnan, China	-	瘤状 Tuberculate	1P

物种 Species	标本号及采集地 Vouchers and locality	赤道轴 × 极轴 Equatorial diam × polar diam (µm)	孢子纹饰 Spore ornamentation	图1中位置 Position in Fig. 1
A. latifolium	Zxl9882. 泰国曼谷植物标本馆 Bangkok Herbarium, Bangkok, Thailand	33.5 × 27.0	粗糙 Scabrate	1A
扇叶铁线蕨 A. flabellulatum	Zxl10018. 中国广东南岭国家森林公园 Nanling National Forest Park, Guangdong, China	45.5 × 31.2	颗粒 Granulate	1B
团羽铁线蕨 A. capillus-junonis	Zxl9959. 中国广东南岭国家森林公园 Nanling National Forest Park, Guangdong, China	46.5 × 43.0	光滑 Psilate	1C
半月形铁线蕨 A. philippense	Yan12405, Yan12086. 中国云南元江; Zxl9839. 泰国彭世洛府屯萨朗峦国家公园 Yan12405, Yan12086. Yuanjiang, Yunnan, China; Zxl9839. Thung Salaeng Luang National Park, Phitsanulok, Thailand	33.4 × 25.7	粗糙 Scabrate	1D
孟连铁线蕨 A. menglianense	Yan12415, Yan12409. 中国云南元江 Yuanjiang, Yunnan, China	31.5 × 26.6	颗粒 Granulate	1E
翅柄铁线蕨 A. soboliferum	Yan11393. 中国海南昌江 Changjiang, Hainan, China	34.0 × 30.5	颗粒 Granulate	1F
A. zollingeri	Zxl9812, Zxl9911, ZXL9851. 泰国碧武里府康卡沾国家公园 Kaeng Krachan National Park, Phetchaburi, Thailand	34.0 × 27.3	瘤状 Tuberculate	1G
鞭叶铁线蕨 A. caudatum	Yan12408. 中国云南元江 Yuanjiang, Yunnan, China	40.6 × 33.5	瘤状 Tuberculate	1H
白垩铁线蕨 A. gravesii	WYG393. 中国贵州贞丰 Zhenfeng, Guizhou, China	51.5 × 36.1	瘤状 Tuberculate	1I
小铁线蕨 A. mariesii	Zxl9685. 中国湖南桑植 Sangzhi, Hunan, China	36.8 × 30.1	疣状 Verrucate	1J
仙霞铁线蕨 A. juxtapositum	Yan1205071. 中国广东仁化 Renhua, Guangdong, China	60.4 × 45.7	疣状 Verrucate	1K
普通铁线蕨 A. edgewothii	Yan12412. 中国云南元江 Yuanjiang, Yunnan, China	30.0 × 25.6	瘤状 Tuberculate	1L
假鞭叶铁线蕨 A. malesianum	Yan12416, Yan12404. 中国云南元江 Yuanjiang, Yunnan, China	38.8 × 32.8	瘤状 Tuberculate	1M
梅山铁线蕨 A. × meishanianum	Yan12411. 中国云南元江 Yuanjiang, Yunnan, China	-	瘤状 Tuberculate	1N
苍山铁线蕨 A. sinicum	Yan112601, Yan12414. 中国云南元江 Yuanjiang, Yunnan, China	31.7 × 29.1	瘤状 Tuberculate	1O
哀牢山铁线蕨 A. × ailaoshanense	Yan12413, Yan12410. 中国云南元江 Yuanjiang, Yunnan, China	-	瘤状 Tuberculate	1P

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祖先性状重建法揭示铁线蕨属植物孢子表面纹饰的形态多样性及其演化

Ancestral state reconstruction reveals the diversity and evolution of spore ornamentation in Adiantum (Pteridaceae)

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