中国与欧洲高卢蜜环菌的遗传多样性

doi:10.3724/SP.J.1003.2012.07214

生物多样性 ›› 2012, Vol. 20 ›› Issue (2): 224-230. DOI: 10.3724/SP.J.1003.2012.07214

中国与欧洲高卢蜜环菌的遗传多样性

孙立夫¹^,^*(), 裴克全², 张艳华¹, 赵俊³, 杨国亭⁴, 秦国夫³, 宋玉双³, 宋瑞清⁴

1 绍兴文理学院生命科学学院, 浙江绍兴 312000
2 中国科学院植物研究所植被与环境变化国家重点实验室, 北京 100093
3 国家林业局森林病虫害防治总站, 沈阳 110034
4 东北林业大学林学院, 哈尔滨 150040

收稿日期:2011-11-24 接受日期:2012-02-28 出版日期:2012-03-20 发布日期:2012-04-09
通讯作者: 孙立夫
作者简介:* E-mail: sunlifu2000@163.com
基金资助:
浙江省自然科学基金(Y3090409);哈尔滨市青年科学基金(2002AFQXJ006)

Genetic diversity of Armillaria gallica isolates from China and Europe revealed with ISSR analysis

Lifu Sun¹^,^*(), Kequan Pei², Yanhua Zhang¹, Jun Zhao³, Guoting Yang⁴, Guofu Qin³, Yushuang Song³, Ruiqing Song⁴

1 College of Life Sciences, Shaoxing University, Shaoxing, Zhejiang 312000
2 State Key Laboratory of Vegetation and Environmental Change, Institute of Botany, Chinese Academy of Sciences, Beijing 100093
3 General Station of Forest Pest Management, State Forestry Administration, Shenyang 110034
4 School of Forestry, Northeast Forestry University, Harbin 150040

Received:2011-11-24 Accepted:2012-02-28 Online:2012-03-20 Published:2012-04-09
Contact: Lifu Sun

1. 附录I 中国和欧洲的高卢蜜环菌遗传多样性参试菌株表.pdf(171KB)

摘要/Abstract

摘要：

高卢蜜环菌(Armillaria gallica)为北半球广布种, 不同大陆间的菌株遗传相似性和多样性水平能反映出该种在洲际大陆尺度上的地理遗传变异关系。作者用ISSR(inter-simple sequence repeat)分子标记技术, 对从中国和欧洲收集到的高卢蜜环菌79个菌株进行了遗传多样性分析。用6个ISSR引物扩增得到210个位点, 其中多态性位点(频率<0.95)为202个, 占96.2%, 平均每个引物多态位点多达33.6个, 表明ISSR标记在蜜环菌中存在较高的多态性。根据非加权类平均法(UPGMA)聚类分析, 中国53个菌株中的49个在0.773的相似性水平上聚成了中国类群(China group); 而欧洲26个菌株遗传分化较大, 分别在0.775和0.763的相似性水平上聚成了欧洲类群A(Europe group A)和B(Europe group B); 2个欧洲类群间的相似性水平仅为0.738, 而欧洲类群A与中国类群间的相似性却达到了0.770; 两个大陆均有少数菌株表现出较为明显的遗传分化, 个别菌株的种内遗传相似性甚至低于蜜环菌种间的遗传相似性。结果表明, 中欧两个大陆间的A. gallica菌株因地理隔离已经表现出明显的遗传分化, 处于异域物种形成过程中; 欧洲大陆的菌株遗传分化更为明显, 可能是两个大陆A. gallica菌株的起源地。

关键词: Armillaria gallica, 遗传多样性, 遗传相似性, 聚类分析, 地理隔离

Abstract

Armillaria gallica is a cosmopolitan fungal species found in the northern hemisphere, and genetic diversity and similarity between different continental isolates of the species can reveal important large-scale biogeographical patterns. A total of 79 isolates of A. gallica were collected from Europe and China. We estimated the genetic diversity of these isolates using ISSR (inter-simple sequence repeat) markers. Six ISSR primers were employed to identify a total of 210 scorable fragments, of which 202 (96.2%) were polymorphic loci (frequecy<0.95). The average number of polymorphic loci detected with each primer was 33.6. The results indicated that ISSR markers can reflect abundant polymorphism in Armillaria. According to UPGMA clustering analysis, 49 out of 53 Chinese A. gallica isolates clustered into a China Group at the 0.773 similarity level. The genetic divergence of European isolates was significantly higher than predicted; these isolates were clustered into Europe Group A and Group B at the 0.775 and 0.763 similarity level, respectively. The similarity coefficient between the two European groups was only 0.738, but it was 0.770 between China Group and Europe Group A. Some isolates from China and Europe had experienced obvious genetic divergence, and several isolates had lower intraspecific similarity coefficients than interspecific similarity coefficient found in other Armillaria biological species. Our results indicated that considerable genetic differentiation existed between European and Chinese isolates due to geographic isolation, and that these isolates were in the process of allopatric speciation. The differentiation among isolates of A. gallica from Europe was more evident, and Europe is possibly the place of origin for isolates of the species.

Key words: Armillaria gallica, genetic diversity, genetic similarity, clustering analysis, geographic isolation

孙立夫, 裴克全, 张艳华, 赵俊, 杨国亭, 秦国夫, 宋玉双, 宋瑞清 (2012) 中国与欧洲高卢蜜环菌的遗传多样性. 生物多样性, 20, 224-230. DOI: 10.3724/SP.J.1003.2012.07214.

Lifu Sun, Kequan Pei, Yanhua Zhang, Jun Zhao, Guoting Yang, Guofu Qin, Yushuang Song, Ruiqing Song (2012) Genetic diversity of Armillaria gallica isolates from China and Europe revealed with ISSR analysis. Biodiversity Science, 20, 224-230. DOI: 10.3724/SP.J.1003.2012.07214.

导出引用管理器 EndNote|Ris|BibTeX

链接本文: https://www.biodiversity-science.net/CN/10.3724/SP.J.1003.2012.07214

https://www.biodiversity-science.net/CN/Y2012/V20/I2/224

图/表 2

表1 ISSR引物及退火温度

Table 1 The primers of ISSR and their annealing temperature

引物序号(编号) Primers	引物序列 Primer sequence	退火温度 Annealing temperature (℃)
9#(MAO)	(CTC)₄RC	48
11#(GUO)	BDB(ACA)₅	48
13#(857)	(CA)₈YG	54
14#(MANNY)	(CAC)₄RC	60
15#(TCG)	XDH(TCG)₅	61
16#(CGA)	DHB(CGA)₅	61

图1 中国和欧洲大陆高卢蜜环菌的聚类树状图(I: 中国类群; II: 欧洲类群A; III: 欧洲类群B)

Fig. 1 Dendrogram based on similarity between Armillaria gallica isolates from Europe and China. I, China group; II, Europe group A; III, Europe group B

参考文献 40

[1]	Anderson JB, Korhonen K, Ullrich RC (1980) Relationships between European and North American biological species of Armillaria mellea. Experimental Mycology, 4, 78-86. DOI URL
[2]	Antonín V, Tomšovský M, Sedlák P, Májek T, Jankovský L (2009) Morphological and molecular characterization of the Armillaria cepistipes - A. gallica complex in the Czech Republic and Slovakia. Mycological Progress, 8, 259-271. DOI URL
[3]	Banik MT, Burdsall HH Jr. (1998) Assessment of compatibility among Armillaria cepistipes, A. sinapina, and North American biological species X and XI, using culture morphology and molecular biology. Mycologia, 90, 798-805.
[4]	Baumgartner K, Travadon R, Bruhn J, Bergemann SE (2010) Contrasting patterns of genetic diversity and population structure of Armillaria mellea sensu stricto in the eastern and western United States. Phytopathology, 100, 708-718. URL PMID
[5]	Bérubé JA, Dessureault M, Berthelay S, Guillaumin JJ (1996) Interfertility between Armillaria cepistipes and A. sinapina. Phytoprotection, 77, 67-74.
[6]	Cha JY, Igarashi T (1995) Armillaria species associated with Gastrodia elata in Japan. European Journal of Forest Pathology, 25, 319-326.
[7]	Chillali M, Idder-Ighili H, Guillaumin JJ, Mohammed C, Escarmant BL, Botton B (1998a) Variation in the ITS and IGS regions of ribosomal DNA among the biological species of European Armillaria. Mycological Research, 102, 533-540.
[8]	Chillali M, Wipf D, Guillaumin JJ, Mohammed C, Botton B (1998b) Delineation of the European Armillaria species based on the sequences of the internal transcribed spacer (ITS) of ribosomal DNA. New Phytologist, 138, 553-561.
[9]	Coetzee MPA, Wingfield BD, Bloomer P, Ridley GS, Wingfield MJ (2003) Molecular identification and phylogeny of Armillaria isolates from South America and Indo-Malaysia. Mycologia, 95, 285-293. URL PMID
[10]	Coetzee MPA, Wingfield BD, Harrington TC, Dalevi D, Coutinho TA, Wingfield MJ (2000) Geographical diversity of Armillaria mellea s. s. based on phylogenetic analysis. Mycologia, 92, 105-113.
[11]	Dai YC (戴玉成), Qin GF (秦国夫), Xu MQ (徐梅卿) (2000) The forest pathogens of root and butt rot in Northeast China. Forest Research (林业科学研究), 13, 15-22. (in Chinese with English abstract)
[12]	Dai YC (戴玉成), Zhou LW (周丽伟), Yang ZL (杨祝良), Wen HA (文华安), Bau T (图力古尔), Li TH (李泰辉) (2010) A revised checklist of edible fungi in China. Mycosystema (菌物学报), 29, 1-21. (in Chinese with English abstract)
[13]	Gatto AG, Sicoli G, Luisi N (2009) Genetic diversity within an Italian population of forest Armillaria gallica isolates as assessed by RAPD-PCR analysis. Journal of Phytopathology, 157, 94-100. DOI URL
[14]	Gregory SC, Rishbeth J, Shaw CG III (1991) Pathogenicity and virulence. In: Armillaria Root Disease (eds Shaw CG, Kile GA). pp. 76-87. USDA Forest Service, Washington, DC.
[15]	Hamrick JL, Loveless MD (1989) Associations between the breeding system and the genetic structure of tropical tree populations. In: Evolutionary Ecology of Plants (eds Bock J, Linhart YB), pp. 129-146. West View Press, Boulder.
[16]	Keča N, Bodles WJA, Woodward S, Karadzic D, Bojovic S. (2006) Molecular-based identification and phylogeny of Armillaria species from Serbia and Montenegro. Forest Pathology, 36, 41-57.
[17]	Keča N, Solheim H (2011) Ecology and distribution of Armillaria species in Norway. Forest Pathology, 41, 120-132.
[18]	Kile GA, Guillaumin JJ, Mohammed C, Watling R (1994) Biogeography and pathology of Armillaria. In: Proceeding of the Eighth International Conference on Root Rot and Butt Rots (eds Jonhansson M, Stenlid J), pp. 411-436. Wik, Sweden and Haikko, Finland, August 9-16, 1993. Swedish University of Agricultural Science, Uppsala, Sweden.
[19]	Kile GA, McDonald GI, Byler JW (1991) Ecology and disease in natural forests. In: Armillaria Root Disease (eds Shaw CG, Kile GA), pp. 102-121. USDA Forest Service, Washington, DC.
[20]	Kim HJ (1997) Selection of superior strains of Armillaria species for the cultivation of Gastrodia elata. FRI Journal of Forest Science (Seoul), 56, 44-51.
[21]	McKinnon JS, Mori S, Blackman BK, David L, Kingsley DM, Jamieson L, Chou J, Schluter D (2004) Evidence for ecology’s role in speciation. Nature, 429, 294-298. DOI URL
[22]	Peterson RH (1995) There’s more to a mushroom than meets the eye: mating studies in the Agaricales. Mycologia, 87, 1-17. DOI URL
[23]	Pildain MB, Coetzee MPA, Rajchenberg M, Petersen RH, Wingfield MJ, Wingfield BD (2009) Molecular phylogeny of Armillaria from the Patagonian Andes. Mycological Progress, 8, 181-194. DOI URL
[24]	Qin GF (秦国夫), Zhao J (赵俊), Tian SM (田淑敏), Hantula J (2001) Genetic diversity and molecular identification of Northern Hemisphere species of Armillaria gallica. Scientia Silvae Sinicae (林业科学), 37(2), 61-70. (in Chinese with English abstract) DOI URL
[25]	Qin GF (秦国夫), Zhao J (赵俊), Tian SM (田淑敏), Korhonen K, Dai YC (戴玉成), Liu CR (刘春如) (2000) New biological species of Armillaria in China. Mycosystema (菌物学报), 19, 509-516. (in Chinese with English abstract)
[26]	Qin GF (秦国夫) (2002) Genetic and phylogenetic studies on Chinese Armillaria species (中国蜜环菌的遗传学与系统学研究). PhD dissertation, Chinese Academy of Forestry, Beijing. (in Chinese with English abstract)
[27]	Rohlf FJ (2002) NTSYS-pc: Numerical Taxonomy System. Version 2.1. Exeter Publishing, Ltd., Setauket, New York.
[28]	Saville BJ, Yoell H, Anderson JB (1996) Genetic exchange and recombination in populations of the root-infecting fungus Armillaria gallica. Molecular Ecology, 5, 485-497. DOI URL PMID
[29]	Sun LF (孙立夫), Pei KQ (裴克全), Zhang YH (张艳华), Zhao J (赵俊), Yang GT (杨国亭), Qin GF (秦国夫), Song YS (宋玉双), Song RQ (宋瑞清) (2011) Genetic diversity of Armillaria gallica revealed by ISSR analysis. Mycosystema (菌物学报), 30, 686-694. (in Chinese with English abstract)
[30]	Sun LF (孙立夫), Zhang YH (张艳华), Yang GT (杨国亭), Qin GF (秦国夫), Song YS (宋玉双), Song RQ (宋瑞清) (2007a) A review of biological species of Armillaria and their geographic distribution. Mycosystema (菌物学报), 26, 305-316. (in Chinese with English abstract)
[31]	Sun LF (孙立夫), Zhang YH (张艳华), Yang GT (杨国亭), Qin GF (秦国夫), Song YS (宋玉双), Song RQ (宋瑞清), Zhao J (赵俊), Tian SM (田淑敏) (2007b) Geographic distribution survey of Armillaria biological species in Heilongjiang Province. Mycosystema (菌物学报), 26, 59-67. (in Chinese with English abstract)
[32]	Sun LF (孙立夫), Zhang YH (张艳华), Pei KQ (裴克全) (2009a) A rapid extraction of genomic DNA from fungi. Mycosystema (菌物学报), 28, 299-302. (in Chinese with English abstract)
[33]	Sun LF (孙立夫), Zhang YH (张艳华), Pei KQ (裴克全), Yang GT (杨国亭), Song YS (宋玉双), Qin GF (秦国夫), Song RQ (宋瑞清) (2009b) Optimization of inter-simple sequence repeat (ISSR-PCR) reaction system for Armillaria gallica. Mycosystema (菌物学报), 28, 261-267. (in Chinese with English abstract)
[34]	Sun LF (孙立夫), Zhang YH (张艳华), Pei KQ (裴克全), Yang GT (杨国亭), Song YS (宋玉双), Song RQ (宋瑞清) (2012) Phylogenetic analysis of Armillaria gallica from North America and China. Mycosystema (菌物学报), 31, 174-186. (in Chinese with English abstract)
[35]	Sung JM, Jung BS, Yang KJ, Lee HK, Harrington TC (1995) Production of Gastrodia elata tuber using Armillaria spp. Korean Journal of Mycology, 23, 61-70.
[36]	Vilgalys R (1991) Speciation and species concepts in the Collybia dryophila complex. Mycologia, 83, 758-773. DOI URL
[37]	Wei YL (魏玉莲), Dai YC (戴玉成) (2004) Ecological function of wood-inhabiting fungi in forest ecosystem. Chinese Journal of Applied Ecology (应用生态学报), 15, 1935-1938. (in Chinese with English abstract)
[38]	Zervakis GI, Moncalvo JM, Vilgalys R (2004) Molecular phylogeny, biogeography and speciation of the mushroom species Pleurotus cystidiosus and allied taxa. Microbiology, 150, 715-726. DOI URL PMID
[39]	Zhang Y (张昀) (2003) Evolution of Biology (生物进化). Peking University Press, Beijing. (in Chinese)
[40]	Zhao J (赵俊), Dai YC (戴玉成), Qin GF (秦国夫), Wei YL (魏玉莲), Wang HC (王汉臣), Yuan HS (袁海生) (2008) New biological species of Armillaria from China. Mycosystema (菌物学报), 27, 156-170. (in Chinese with English abstract)

中国与欧洲高卢蜜环菌的遗传多样性

Genetic diversity of Armillaria gallica isolates from China and Europe revealed with ISSR analysis

RichHTML

PDF (PC)

补充材料

可视化

被引次数

摘要/Abstract

引用本文

使用本文

图/表 2

参考文献 40

相关文章 15

编辑推荐

Metrics

本文评价

[1]	吴相獐, 雷富民, 单壹壹, 于晶. 上海城市公园苔藓植物多样性分布格局及其环境影响因子[J]. 生物多样性, 2024, 32(2): 23364-.
[2]	李庆多, 栗冬梅. 全球蝙蝠巴尔通体流行状况分析[J]. 生物多样性, 2023, 31(9): 23166-.
[3]	冯晨, 张洁, 黄宏文. 统筹植物就地保护与迁地保护的解决方案: 植物并地保护(parallel situ conservation)[J]. 生物多样性, 2023, 31(9): 23184-.
[4]	齐海玲, 樊鹏振, 王跃华, 刘杰. 中国北方六省区胡桃的遗传多样性和群体结构[J]. 生物多样性, 2023, 31(8): 23120-.
[5]	熊飞, 刘红艳, 翟东东, 段辛斌, 田辉伍, 陈大庆. 基于基因组重测序的长江上游瓦氏黄颡鱼群体遗传结构[J]. 生物多样性, 2023, 31(4): 22391-.
[6]	蒲佳佳, 杨平俊, 戴洋, 陶可欣, 高磊, 杜予州, 曹俊, 俞晓平, 杨倩倩. 长江下游外来生物福寿螺的种类及其种群遗传结构[J]. 生物多样性, 2023, 31(3): 22346-.
[7]	金恒镳. 从天择到人择: 在华莱士的肩膀上看地球的未来[J]. 生物多样性, 2023, 31(12): 23267-.
[8]	何艺玥, 刘玉莹, 张富斌, 秦强, 曾燏, 吕振宇, 杨坤. 梯级水利工程背景下的嘉陵江干流蛇鮈群体遗传多样性和遗传结构[J]. 生物多样性, 2023, 31(11): 23160-.
[9]	孙维悦, 舒江平, 顾钰峰, 莫日根高娃, 杜夏瑾, 刘保东, 严岳鸿. 基于保护基因组学揭示荷叶铁线蕨的濒危机制[J]. 生物多样性, 2022, 30(7): 21508-.
[10]	陶克涛, 白东义, 图格琴, 赵若阳, 安塔娜, 铁木齐尔·阿尔腾齐米克, 宝音德力格尔, 哈斯, 芒来, 韩海格. 基于基因组SNPs对东亚家马不同群体遗传多样性的评估[J]. 生物多样性, 2022, 30(5): 21031-.
[11]	崔静, 徐明芳, 章群, 李瑶, 曾晓舒, 李莎. 基于3种线粒体标记探讨中日沿海角木叶鲽遗传多样性差异[J]. 生物多样性, 2022, 30(5): 21485-.
[12]	蔡新宇, 毛晓伟, 赵毅强. 家养动物驯化起源的研究方法与进展[J]. 生物多样性, 2022, 30(4): 21457-.
[13]	孙军, 宋煜尧, 施义锋, 翟键, 燕文卓. 近十年中国海洋生物多样性研究进展[J]. 生物多样性, 2022, 30(10): 22526-.
[14]	栗冬梅, 杨卫红, 李庆多, 韩茜, 宋秀平, 潘虹, 冯云. 巴尔通体在滇西南蝙蝠中高度流行并具有丰富的遗传变异特征[J]. 生物多样性, 2021, 29(9): 1245-1255.
[15]	刘璐, 迟瑶, 吴朝宁, 钱天陆, 王结臣. 陆栖哺乳动物的地理隔离研究进展[J]. 生物多样性, 2021, 29(8): 1134-1145.