基于SSR标记分析小豆及其近缘植物的遗传关系

doi:10.3724/SP.J.1003.2011.07155

摘要/Abstract

摘要：

本研究利用87对SSR引物分析了80份栽培小豆(Vigna angularis)、22份野生小豆(V. angularis var. nipponensis)以及10份豇豆属(共7个种)近缘植物, 旨在比较豇豆属不同种的遗传多样性, 并分析种间的遗传关系。结果显示87对SSR引物在112份小豆及其近缘植物资源中共检测到667个等位变异, 其中有75个、71个和82个SSR位点分别在栽培小豆、野生小豆和近缘植物中表现为多态。随机抽样分析发现, 平均每SSR位点检测到的等位变异数目为近缘植物>野生小豆>栽培小豆, 与多态信息含量(PIC)值一致, 说明近缘植物及野生小豆中蕴含着丰富的遗传变异, 是栽培小豆育种的重要基因来源。聚类分析显示, 栽培小豆、野生小豆和近缘植物间的遗传分化比较明显, 分别聚为三大类, 其中栽培小豆的遗传背景与其生态环境相对应; 近缘植物又可以分为三个亚类, 亚类间的遗传距离与其亲缘关系相对应。本研究结果也说明利用SSR标记辅助豇豆属的种间分类是可行的。

关键词: 豇豆属植物, SSR, 遗传多样性, 遗传关系

Abstract

In order to enhance the use of genetic resources of Vigna in breeding of adzuki bean, genetic diversity and relationship were analyzed based on 87 SSR markers. The results showed 667 alleles were detected at these SSR loci among 112 accessions of Vigna plants, including 80 cultivated, 22 wild adzuki bean and 10 relative plants. And 75, 71 and 82 were polymorphic in cultivated, wild adzuki bean and relative plants, respectively. Random sampling analysis suggested that the relative plants ranked the first for the average number of alleles per SSR locus, and wild adzuki bean ranked the second, this was agreeable with polymorphism information content (PIC). Cluster analysis divided the cultivated, wild adzuki bean and relative plants into three distinct groups. The genetic background of cultivated adzuki bean basically agreed with their origins. The relative plants could also be divided into three sub-groups, and V. umbellata had different genetic background with other wild species. The present study suggested that wild Vigna had higher genetic variations than cultivated adzuki bean, and could be used to broadening the gene pool of this crop.

Key words: adzuki bean, Vigna, genetic diversity, SSR

王丽侠, 程须珍, 王素华 (2011) 基于SSR标记分析小豆及其近缘植物的遗传关系. 生物多样性, 19, 17-23. DOI: 10.3724/SP.J.1003.2011.07155.

Lixia Wang, Xuzhen Cheng, Suhua Wang (2011) Genetic diversity in adzuki bean and its relatives based on SSR markers. Biodiversity Science, 19, 17-23. DOI: 10.3724/SP.J.1003.2011.07155.

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链接本文: https://www.biodiversity-science.net/CN/10.3724/SP.J.1003.2011.07155

https://www.biodiversity-science.net/CN/Y2011/V19/I1/17

图/表 4

表1 小豆及其近缘植物的类别及受检种质数

Table 1 Number of accessions detected for different species in Vigna

种属 Species	数量 Amount
Vigna angularis	80
V. angularisvar.nipponensis	22
V. umbellata	3
V. hirtella	1
V. tenuicaulis	1
V. riukivernsis	2
V. minima	1
V. trinervia	1
V. nakashimae	1
合计 Total	112

图1 667个等位变异在不同小豆或近缘植物群体间的分布。下划线者为群体特有等位变异, 黑体为两两群体间特有等位变异, 斜体为3个群体间共有等位变异。

Fig. 1 Distribution of 667 alleles among cultivated, wild adzuki bean and other relatives. The underlined number is the specific alleles within each population, the bold numbers are the common alleles between populations, and the italic number is the specific alleles among the three populations.

图2 基于小豆及其近缘植物间遗传相似性系数绘制的树状聚类图

Fig. 2 Dendrogram showing genetic relationship among adzuki bean and its relatives based on coefficient of similarity

图3 基于主成分分析绘制的小豆及其近缘植物关系的二维坐标图

Fig. 3 Two-dimentional representaion of genetic relationship among adzuki bean and its relatives

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