生物多样性 ›› 2021, Vol. 29 ›› Issue (9): 1236-1244. DOI: 10.17520/biods.2021034
收稿日期:
2021-01-25
接受日期:
2021-04-22
出版日期:
2021-09-20
发布日期:
2021-05-28
通讯作者:
张爱梅
作者简介:
* E-mail: zhangaimei@nwnu.edu.cn基金资助:
Aimei Zhang(), Yiran Yin, Weibao Kong, Xuetai Zhu, Yingli Yang
Received:
2021-01-25
Accepted:
2021-04-22
Online:
2021-09-20
Published:
2021-05-28
Contact:
Aimei Zhang
摘要:
西藏沙棘(Hippophae tibetana)是分布于高寒高海拔地区的一类特殊的放线菌结瘤植物, 弗兰克氏菌能够侵染其根部形成根瘤, 因共生固氮等作用而增强其生态适应性。在西藏沙棘的根瘤中, 除了弗兰克氏菌之外还有其他内生菌, 而弗兰克氏菌又不仅仅在根瘤中有分布。为了探究弗兰克氏菌在西藏沙棘不同组织中的定殖及可能的迁移规律, 分析不同组织中内生细菌的群落结构及多样性, 本研究以生长在甘肃省天祝藏族自治县抓喜秀龙金强河河滩地的西藏沙棘为材料, 应用16S rRNA扩增子高通量测序技术, 对西藏沙棘根瘤、茎、枝、叶和种子等不同组织的内生细菌多样性进行了分析。研究结果表明, 西藏沙棘根瘤内生细菌群落丰富度及多样性最高, 种子内生细菌群落丰富度最低, 茎内生细菌群落多样性最低。西藏沙棘5种不同组织中的弗兰克氏菌和其他内生细菌多样性都具有一定差异, 变形菌门均为优势门, 弗兰克氏菌属(Frankia)为根瘤内生细菌群落的优势属, 弗莱德门菌属(Friedmanniella)为茎内生细菌群落的优势属, 寡养单胞菌属(Stenotrophomonas)为枝、叶和种子内生细菌群落的优势属。研究结果还表明, 弗兰克氏菌属不仅仅存在于西藏沙棘的根瘤, 还能够分布于其他组织, 且在同一种组织中存在弗兰克氏菌属的不同“种”; 而在西藏沙棘不同组织中, 也分布有弗兰克氏菌属的相同“种”。此外, 对西藏沙棘5种不同组织内生细菌中的功能菌株的分析表明, 不同组织中均存在着具有固氮、促生和抑菌功能的内生细菌, 但具有固氮作用的内生细菌主要分布于根瘤, 具有促生作用以及抑菌功能的内生细菌主要分布于枝和叶。综上, 西藏沙棘5种不同组织内生细菌具有丰富的多样性, 但各组织内生细菌的群落结构和优势种群有所不同, 且不同组织也能够定殖具有多种功能的内生细菌。
张爱梅, 殷一然, 孔维宝, 朱学泰, 杨颖丽 (2021) 西藏沙棘5种不同组织内生细菌多样性. 生物多样性, 29, 1236-1244. DOI: 10.17520/biods.2021034.
Aimei Zhang, Yiran Yin, Weibao Kong, Xuetai Zhu, Yingli Yang (2021) Diversity of endophytic bacteria in five types of tissues of Hippophae tibetana. Biodiversity Science, 29, 1236-1244. DOI: 10.17520/biods.2021034.
样品 Sample | 门 Phylum | 纲 Class | 目 Order | 科 Family | 属 Genus |
---|---|---|---|---|---|
根瘤 Root nodule | 22 | 33 | 69 | 113 | 203 |
茎 Stem | 18 | 28 | 59 | 102 | 185 |
枝 Branch | 18 | 29 | 58 | 99 | 191 |
叶 Leaf | 18 | 24 | 49 | 91 | 171 |
种子 Seed | 16 | 25 | 55 | 96 | 172 |
表1 西藏沙棘5种组织内生细菌各分类单元的群落组成
Table 1 Endophytic bacterial community in five tissues of Hippophae tibetana at each classification level
样品 Sample | 门 Phylum | 纲 Class | 目 Order | 科 Family | 属 Genus |
---|---|---|---|---|---|
根瘤 Root nodule | 22 | 33 | 69 | 113 | 203 |
茎 Stem | 18 | 28 | 59 | 102 | 185 |
枝 Branch | 18 | 29 | 58 | 99 | 191 |
叶 Leaf | 18 | 24 | 49 | 91 | 171 |
种子 Seed | 16 | 25 | 55 | 96 | 172 |
样品 Sample | ACE指数 ACE index | Chao1指数 Chao1 index | Shannon指数 Shannon index | Simpson指数 Simpson index |
---|---|---|---|---|
根瘤 Root nodule | 1,044±197a | 1,040±197a | 4.633±2.673ab | 0.700±0.283b |
茎 Stem | 605±76a | 575±78a | 6.107±0.173a | 0.969±0.004a |
枝 Branch | 307±42ab | 285±34b | 2.820±0.664b | 0.646±0.147b |
叶 Leaf | 296±63b | 279±60b | 3.340±1.337a | 0.753±0.250ab |
种子 Seed | 265±2b | 260±7b | 3.007±0.245b | 0.751±0.020b |
表2 西藏沙棘5种不同组织内生细菌群落丰富度及多样性指数
Table 2 Richness and diversity indices of endophytic bacteria in five tissues of Hippophae tibetana
样品 Sample | ACE指数 ACE index | Chao1指数 Chao1 index | Shannon指数 Shannon index | Simpson指数 Simpson index |
---|---|---|---|---|
根瘤 Root nodule | 1,044±197a | 1,040±197a | 4.633±2.673ab | 0.700±0.283b |
茎 Stem | 605±76a | 575±78a | 6.107±0.173a | 0.969±0.004a |
枝 Branch | 307±42ab | 285±34b | 2.820±0.664b | 0.646±0.147b |
叶 Leaf | 296±63b | 279±60b | 3.340±1.337a | 0.753±0.250ab |
种子 Seed | 265±2b | 260±7b | 3.007±0.245b | 0.751±0.020b |
图2 西藏沙棘5种不同组织内生细菌相对丰度。(A)门水平; (B)属水平。
Fig. 2 Relative abundance of endophytic bacteria in five tissues of Hippophae tibetana. (A) Phylum level; (B) Genus level.
样品 Sample | OTU3 | OTU88 | OTU241 | OTU1030 | OTU1524 | OTU1958 | OTU2669 |
---|---|---|---|---|---|---|---|
根瘤 Root nodule | 45.04% | 1.07% | 1.47% | 0.01% | 0.03% | 0.01% | 0.01% |
茎 Stem | 0.12% | 0.01% | 0.01% | 0 | 0.01% | 0 | 0 |
枝 Branch | 0.02% | 0 | 0.01% | 0 | 0 | 0 | 0 |
叶 Leaf | 0.02% | 0.01% | 0.01% | 0 | 0 | 0 | 0 |
种子 Seed | 0.02% | 0.01% | 0.01% | 0 | 0 | 0 | 0 |
表3 弗兰克氏菌OTU在西藏沙棘5种不同组织内的分布
Table 3 Distribution of Frankia OTU in five tissues of Hippophae tibetana
样品 Sample | OTU3 | OTU88 | OTU241 | OTU1030 | OTU1524 | OTU1958 | OTU2669 |
---|---|---|---|---|---|---|---|
根瘤 Root nodule | 45.04% | 1.07% | 1.47% | 0.01% | 0.03% | 0.01% | 0.01% |
茎 Stem | 0.12% | 0.01% | 0.01% | 0 | 0.01% | 0 | 0 |
枝 Branch | 0.02% | 0 | 0.01% | 0 | 0 | 0 | 0 |
叶 Leaf | 0.02% | 0.01% | 0.01% | 0 | 0 | 0 | 0 |
种子 Seed | 0.02% | 0.01% | 0.01% | 0 | 0 | 0 | 0 |
图5 西藏沙棘5种不同组织中功能内生细菌的种类及丰度。(A)固氮; (B)促生; (C)抑制寄主植物病原体。
Fig. 5 Classification and relative abundance of functional endophytic bacteria in five tissues of Hippophae tibetana. (A) Nitrogen fixation; (B) Growth-promoting; (C) Inhibiting plant pathogens.
[1] |
Afzal I, Shinwari ZK, Sikandar S, Shahzad S (2019) Plant beneficial endophytic bacteria: Mechanisms, diversity, host range and genetic determinants. Microbiological Research, 221, 36-49.
DOI URL |
[2] |
Beckers B, Beeck MOD, Weyens N, Boerjan W, Vangronsveld J (2017) Structural variability and niche differentiation in the rhizosphere and endosphere bacterial microbiome of field-grown poplar trees. Microbiome, 5, 25.
DOI PMID |
[3] | Cai XY, Liu YC, Xie ZH, Chen YL, Liu RJ (2020) Research advances in mutualistic symbiotic microbes diversities. Microbiology China, 47, 3899-3917. (in Chinese with English abstract) |
[蔡昕悦, 刘耀臣, 解志红, 陈应龙, 刘润进 (2020) 互惠共生微生物多样性研究概况. 微生物学通报, 47, 3899-3917.] | |
[4] | Cao K, Wang XN, Sun YF, Li ZW, Pan DM, Zhao Y (2017) Research progress on endophytic bacterial diversity and biocontrol of plant roots. Agriculture and Technology, 37(17), 1-3, 5. (in Chinese) |
[曹焜, 王晓楠, 孙宇峰, 李振伟, 潘冬梅, 赵越 (2017) 植物根部内生细菌多样性及其生防作用研究进展. 农业与技术, 37(17), 1-3, 5.] | |
[5] |
Chaia EE, Wall LG, Huss-Danell K (2010) Life in soil by the actinorhizal root nodule endophyte Frankia. A review. Symbiosis, 51, 201-226.
DOI URL |
[6] |
Chen ZY, Liu J, Yang XP, Liu M, Wang Y, Zhang ZB, Zhu D (2019) Community composition and diversity of cultivable endophytic bacteria isolated from Dongxiang wild rice. Biodiversity Science, 27, 1320-1329. (in Chinese with English abstract)
DOI URL |
[陈志远, 刘珺, 杨星鹏, 刘梦, 汪涯, 张志斌, 朱笃 (2019) 东乡野生稻可培养内生细菌群落组成及多样性. 生物多样性, 27, 1320-1329.]
DOI |
|
[7] | Du XN, Xu HJ, Huang PP, Dai JX (2015) Diversity and antimicrobial activity of endophytic bacteria isolated from Lycium barbarum of Ningxia. Microbiology China, 42, 1779-1787. (in Chinese with English abstract) |
[杜晓宁, 徐惠娟, 黄盼盼, 代金霞 (2015) 宁夏枸杞内生细菌的多样性及其抑菌活性研究. 微生物学通报, 42, 1779-1787.] | |
[8] | Gu MY, Gulinisha S, Zhang ZD, Zhu J, Liu XJ, Tang QY, Outikuer M, Song SQ, Feng L, Tang GM, Xu WL (2021) Diversity and function analysis of endophytic bacterial community in different tissues of Lycium ruthenicum Murr. Acta Microbiologica Sinica, 61, 152-166. (in Chinese with English abstract) |
[顾美英,古丽尼沙·沙依木, 张志东, 朱静, 刘晓静, 唐琦勇, 欧提库尔·玛合水提, 宋素琴, 冯雷, 唐光木, 徐万里 (2021) 黑果枸杞不同组织内生细菌群落多样性. 微生物学报, 61, 152-166.] | |
[9] |
Hallmann J, Quadt-Hallmann A, Mahaffee WF, Kloepper JW (1997) Bacterial endophytes in agricultural crops. Canadian Journal of Microbiology, 43, 895-914.
DOI URL |
[10] | He LM, Ye JR (2014) Endophytic bacteria: Research advances and biocontrol applications. Journal of Nanjing Forestry University (Natural Sciences Edition), 38, 153-159. (in Chinese with English abstract) |
[何玲敏, 叶建仁 (2014) 植物内生细菌及其生防作用研究进展. 南京林业大学学报(自然科学版), 38, 153-159.] | |
[11] |
Kim H, Rim SO, Bae H (2019) Antimicrobial potential of metabolites extracted from ginseng bacterial endophyte Burkholderia stabilis against ginseng pathogens. Biological Control, 128, 24-30.
DOI URL |
[12] |
Marag PS, Suman A (2018) Growth stage and tissue specific colonization of endophytic bacteria having plant growth promoting traits in hybrid and composite maize (Zea mays L.). Microbiological Research, 214, 101-113.
DOI URL |
[13] | Ning CH, Li WB, Liu RJ (2019) Research advances in plant symbiotic actinomyces. Chinese Journal of Ecology, 38, 256-266. (in Chinese with English abstract) |
[宁楚涵, 李文彬, 刘润进 (2018) 植物共生放线菌研究进展. 生态学杂志, 38, 256-266.] | |
[14] | Sha YX (2018) Diversity of bacterial endophytic community in different rice tissues. Acta Microbiologica Sinica, 58, 2216-2228. (in Chinese with English abstract) |
[沙月霞 (2018) 不同水稻组织内生细菌的群落多样性. 微生物学报, 58, 2216-2228.] | |
[15] | Song CJ, Ma KM, Fu BJ, Qu LY, Liu Y (2009) A review on the functions of nitrogen-fixers in terrestrial ecosystems. Acta Ecologica Sinica, 29, 869-877. (in Chinese with English abstract) |
[宋成军, 马克明, 傅伯杰, 曲来叶, 刘杨 (2009) 固氮类植物在陆地生态系统中的作用研究进展. 生态学报, 29, 869-877.] | |
[16] |
Tian CJ, He XY, Zhong Y, Chen JK (2002) Effects of VA mycorrhizae and Frankia dual inoculation on growth and nitrogen fixation of Hippophae tibetana. Forest Ecology and Management, 170, 307-312.]
DOI URL |
[17] | Wang ZW, Ji YL, Chen YG (2015) Studies and biological significances of plant endophytes. Microbiology China, 42, 349-363. (in Chinese with English abstract) |
[王志伟, 纪燕玲, 陈永敢 (2015) 植物内生菌研究及其科学意义. 微生物学通报, 42, 349-363.] | |
[18] | Xu GQ, Liu YX, Cao PX, Liu X (2021) Core microflora and endophytic interaction network of Oxytropis glacialis in Qinghai-Tibet Plateau. Microbiology China, 47, 2746-2758 (in Chinese with English abstract) |
[许国琪, 刘怡萱, 曹鹏熙, 刘星 (2021) 青藏高原冰川棘豆(Oxytropis glacialis)内生菌核心微生物组的界定及其互作网络分析. 微生物学通报, 47, 2746-2758.] | |
[19] |
Xu WF, Wang F, Zhang M, Ou T, Wang RL, Strobel G, Xiang ZH, Zhou ZY, Xie J (2019) Diversity of cultivable endophytic bacteria in mulberry and their potential for antimicrobial and plant growth-promoting activities. Microbiological Research, 229, 126328.
DOI URL |
[20] | Zhang AM, Niu SQ, Sun K, Cao WX, Da WY (2010) Study on the root nodules features of Hippophae and Frankia isolation. Grassland and Turf, 30(2), 43-46. (in Chinese with English abstract) |
[张爱梅, 牛世全, 孙坤, 曹文侠, 达文燕 (2010) 沙棘属植物结瘤特性及Frankia根瘤菌分离. 草原与草坪, 30(2), 43-46.] | |
[21] | Zhang AM, Han XY, Sun K, Zhang SH, Kong WB, Niu SQ, Zhu XT (2018a) Root nodules endophytic and rhizosphere soil bacteria diversity of Hippophae rhamnoides subsp. sinensis based on high-throughput sequencing. Grassland and Turf, 38(2), 49-55. (in Chinese with English abstract) |
[张爱梅, 韩雪英, 孙坤, 张世虎, 孔维宝, 牛世全, 朱学泰 (2018a) 高通量测序分析中国沙棘根瘤与根际土壤细菌多样性. 草原与草坪, 38(2), 49-55.] | |
[22] | Zhang AM, Han XY, Zhang SH, Sun K, Wu JY, Yu J, Guo BM (2018b) Diversity of root nodule endophytic bacteria in Hippophae tibetana at different altitudes in Gannan area. Biotic Resources, 40, 114-119. (in Chinese with English abstract) |
[张爱梅, 韩雪英, 张世虎, 孙坤, 吴菊艳, 于杰, 郭保民 (2018b) 甘南高寒地区不同海拔西藏沙棘根瘤内生菌多样性. 生物资源, 40, 114-119.] | |
[23] | Zhang AM, Han XY, Wang J, Kong WB, Niu SQ, Zhu XT (2019a) Diversity of endophytic bacteria in root nodules of Hippophae rhamnoides in the Maxian Mountains. Acta Ecologica Sinica, 39, 294-301. (in Chinese with English abstract) |
[张爱梅, 韩雪英, 王嘉, 孔维宝, 牛世全, 朱学泰 (2019a) 马衔山中国沙棘根瘤内共生细菌多样性研究. 生态学报, 39, 294-301.] | |
[24] | Zhang AM, Wu JY, Han XY, Kong WB (2019b) Screening and identification of antagonistic and plant-growth promoting endophytic bacteria from Seabuckthorn nodules. Microbiology China, 46, 1041-1051. (in Chinese with English abstract) |
[张爱梅, 吴菊艳, 韩雪英, 孔维宝 (2019b) 沙棘根瘤内生细菌中抑菌促生菌株的筛选和鉴定. 微生物学通报, 46, 1041-1051.] | |
[25] | Zhang AM, Yin YR, Sun K (2020a) Research progress in Frankia spp. associated with Hippophae L. Microbiology China, 47, 3933-3944. (in Chinese with English abstract) |
[张爱梅, 殷一然, 孙坤 (2020) 沙棘属植物弗兰克氏菌研究进展. 微生物学通报, 47, 3933-3944.] | |
[26] | Zhang AM, Guo BM, Han XY, Li XR (2020b) Diversity of endophytic bacteria in seeds of Hippophae rhamnoides subsp. sinensis in two different habitats. Acta Ecologica Sinica, 40, 5247-5257. (in Chinese with English abstract) |
[张爱梅, 郭保民, 韩雪英, 李曦冉 (2020b) 两种不同生境中国沙棘种子内生细菌的多样性. 生态学报, 40, 5247-5257.] | |
[27] | Zhang T, Li Y, Xiong J, Xu XH, Lan CH, Tian BY (2020) Community composition and differential analysis of endophytic bacteria in different niches of tomato. Genomics and Applied Biology, 39, 5558-5566. (in Chinese with English abstract) |
[张婷, 黎烨, 熊娟, 许小红, 蓝灿华, 田宝玉 (2020) 番茄不同生态位内生菌的菌群结构组成和差异性分析, 39, 5558-5566.] |
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