Biodiv Sci ›› 2019, Vol. 27 ›› Issue (12): 1320-1329.DOI: 10.17520/biods.2019219

• Original Papers: Microbial Diversity • Previous Articles     Next Articles

Community composition and diversity of cultivable endophytic bacteria isolated from Dongxiang wild rice

Zhiyuan Chen1,Jun Liu1,Xingpeng Yang1,Meng Liu1,Ya Wang2,Zhibin Zhang1,*(),Du Zhu1,2,*   

  1. 1 Key Laboratory of Protection and Utilization of Subtropical Plant Resources of Jiangxi Province, College of Life Sciences, Jiangxi Normal University, Nanchang 330022
    2 Key Laboratory of Bioprocess Engineering of Jiangxi Province, College of Life Sciences, Jiangxi Science and Technology Normal University, Nanchang 330013
  • Received:2019-07-07 Accepted:2019-10-20 Online:2019-12-20 Published:2020-02-22
  • Contact: Zhang Zhibin,Zhu Du

Abstract:

Endophytic bacteria are essential to the growth and metabolism of their host plants. To better understand the community structure and diversity of endophytic bacteria on plants, we isolated 94 strains of endophytic bacteria from the roots, stems and leaves of Dongxiang wild rice (Oryza rufipogon). Subsequent 16S rRNA sequence alignments and phylogenetic analyses revealed that the 94 strains could be categorized into 17 genera of 14 families in 3 phyla (Proteobacteria, Actinobacteria, and Firmicutes).Bacillus and Microbacterium, accounting for 27.7% and 20.2% of the diversity, respectively, were the two most dominant genera. The distribution and diversity of endophytic bacteria differed across different tissues of Dongxiang wild rice: the roots contained the most endophytic bacteria strains (n = 34, 36.2%), followed by leaves (n = 32, 34.0%), and stems (n = 28, 29.8%). Shannon-Wiener diversity index (H′ = 2.52), Simpson dominance index (D = 0.88) and Pielou evenness index (E = 0.72) were higher in the roots than in the stems or leaves. Meanwhile, the Jaccard similarity index between roots and stems (C = 0.29) was lower than that between roots and leaves (C = 0.47), or that between stems and leaves (C = 0.45). Our efforts to uncover the diversity of endophytic bacteria in Dongxiang wild rice confirms that roots contain the most diverse strains but that the composition of endophytes varies across tissues.

Key words: Oryza rufipogon, endophytes, classification, phylogeny, composition analysis