生物多样性 ›› 2018, Vol. 26 ›› Issue (5): 510-518. DOI: 10.17520/biods.2018065
所属专题: 青藏高原生物多样性与生态安全; 传粉生物学
王玉贤1, 刘左军1,*(), 赵志刚2, 侯盟2, 张小瑞1, 吕婉灵1
收稿日期:
2018-02-27
接受日期:
2018-04-26
出版日期:
2018-05-20
发布日期:
2018-09-11
通讯作者:
刘左军
作者简介:
# 共同第一作者
基金资助:
Yuxian Wang1, Zuojun Liu1,*(), Zhigang Zhao2, Meng Hou2, Xiaorui Zhang1, Wanling Lü1
Received:
2018-02-27
Accepted:
2018-04-26
Online:
2018-05-20
Published:
2018-09-11
Contact:
Liu Zuojun
About author:
# Co-first authors
摘要:
花寿命是指花保持开放且具有功能的时间长度。不同物种的花寿命具有显著的差异, 但一定程度上的可塑性反映了植物对传粉环境变化的适应性。本研究以青藏高原高寒草甸不同海拔(2,900 m和3,600 m)的11种开花植物为研究对象, 通过套袋、补充授粉以及自然授粉处理分别测量了植物的潜在花寿命、最短花寿命以及实际花寿命。分析了不同海拔植物花寿命的变异, 以及对套袋处理(潜在花寿命与实际花寿命之差)和补充授粉处理(实际花寿命与最短花寿命之差)的响应及其可塑性(潜在花寿命与最短花寿命之差)的变异。结果表明: 无论是高海拔还是低海拔, 套袋显著延长了花寿命, 而补充授粉显著缩短了花寿命, 即, 潜在花寿命>实际花寿命>最短花寿命。高海拔群落中植物的实际花寿命和潜在花寿命均显著延长, 而最短花寿命在海拔间差异不显著。相对于低海拔群落, 高海拔植物的花寿命对补充授粉处理更敏感, 花寿命的可塑性更大。本研究说明传粉者稀少的高寒环境中, 植物通过提高花寿命及其可塑性来增加授粉机会, 获得更高的适合度。
王玉贤, 刘左军, 赵志刚, 侯盟, 张小瑞, 吕婉灵 (2018) 青藏高原高寒草甸植物花寿命对传粉环境的响应. 生物多样性, 26, 510-518. DOI: 10.17520/biods.2018065.
Yuxian Wang, Zuojun Liu, Zhigang Zhao, Meng Hou, Xiaorui Zhang, Wanling Lü (2018) Responses of floral longevity to pollination environments in 11 species from two alpine meadows. Biodiversity Science, 26, 510-518. DOI: 10.17520/biods.2018065.
因素 Factor | 花寿命 Floral longevity | |||||
---|---|---|---|---|---|---|
群落1和2 Community 1 & 2 | 群落3和4 Community 3 & 4 | |||||
Df | F | P | Df | F | P | |
截距 Intercept | 1, 10 | 17,634.897 | < 0.001 | 1, 9 | 23,757.518 | < 0.001 |
物种 Species | 10, 434 | 762.000 | < 0.001 | 4, 269 | 1,121.932 | < 0.001 |
海拔 Elevation | 1, 437 | 50.519 | < 0.001 | 1, 270 | 55.844 | < 0.001 |
处理 Treatment | 2, 433 | 200.241 | < 0.001 | 2, 267 | 182.577 | < 0.001 |
物种×海拔 Species × Elevation | 4, 435 | 1.218 | 0.303 | 4, 269 | 1.350 | 0.252 |
物种×处理 Species × Treatment | 18, 433 | 4.776 | < 0.001 | 7, 267 | 5.668 | < 0.001 |
海拔×处理 Elevation × Treatment | 2, 433 | 4.316 | 0.014 | 2, 267 | 4.722 | 0.010 |
物种×海拔×处理 Species × Elevation × Treatment | 7, 433 | 0.716 | 0.659 | 7, 267 | 0.791 | 0.596 |
表1 物种、海拔(低海拔与高海拔)、处理(套袋、补充授粉及自然授粉)及它们的交互作用对群落1和2以及群落3和4花寿命的影响。群落1和2分别指低海拔和高海拔所有研究物种组成的群落, 群落3和4分别指低海拔和高海拔共有的研究物种组成的群落。运用一般线性混合效应模型检验这些因素的显著性并用粗体表示差异显著。
Table 1 The effect of species identity, elevation (low vs. high elevation), and pollination treatments (pollinator exclusion, supplemental pollinated, open-pollinated) and their interactions on flower longevity of studied species in community 1 & 2 (and community 3 & 4 ). The community 1 and 2 refer to the community that composed of all the research species at low and high altitudes, respectively, while the community 3 and 4 refer to the community of the mutual research species at low and high altitudes, respectively. Significant differences are examined from general linear mixed effect models at P < 0.05 and indicated in bold.
因素 Factor | 花寿命 Floral longevity | |||||
---|---|---|---|---|---|---|
群落1和2 Community 1 & 2 | 群落3和4 Community 3 & 4 | |||||
Df | F | P | Df | F | P | |
截距 Intercept | 1, 10 | 17,634.897 | < 0.001 | 1, 9 | 23,757.518 | < 0.001 |
物种 Species | 10, 434 | 762.000 | < 0.001 | 4, 269 | 1,121.932 | < 0.001 |
海拔 Elevation | 1, 437 | 50.519 | < 0.001 | 1, 270 | 55.844 | < 0.001 |
处理 Treatment | 2, 433 | 200.241 | < 0.001 | 2, 267 | 182.577 | < 0.001 |
物种×海拔 Species × Elevation | 4, 435 | 1.218 | 0.303 | 4, 269 | 1.350 | 0.252 |
物种×处理 Species × Treatment | 18, 433 | 4.776 | < 0.001 | 7, 267 | 5.668 | < 0.001 |
海拔×处理 Elevation × Treatment | 2, 433 | 4.316 | 0.014 | 2, 267 | 4.722 | 0.010 |
物种×海拔×处理 Species × Elevation × Treatment | 7, 433 | 0.716 | 0.659 | 7, 267 | 0.791 | 0.596 |
物种 Species | 花寿命 Floral longevity | |||||
---|---|---|---|---|---|---|
低海拔 Low elevation (2,900 m ) | 高海拔 High elevation (3,500 m) | |||||
套袋处理 Pollinator exclusion | 自然授粉 Open pollination | 补充授粉Supplemental pollination | 套袋处理Pollinator exclusion | 自然授粉 Open pollination | 补充授粉Supplemental pollination | |
蒲公英 Taraxacum mongolicum | 5.2 ± 0.8a | 4.7 ± 0.6a | - | 6.5 ± 1.2a | 5.6 ± 0.8b | - |
甘青老鹳草 Geranium pylzowianum | 4.4 ± 0.5a | 3.2 ± 0.4b | 2.8 ± 0.3b | 5.0 ± 0.5a | 3.8 ± 0.3b | 3.0 ± 0.3c |
钝裂银莲花 Anemone obtusiloba | 9.8 ± 0.9a | 8.6 ± 1.1b | 7.6 ± 0.4c | 10.8 ± 0.8a | 9.4 ± 0.7b | 7.5 ± 0.6c |
莓叶委陵菜 Potentilla fragarioides | 3.5 ± 0.4a | 2.8 ± 0.3b | 2.4 ± 0.3c | 3.8 ± 0.4a | 3.3 ± 0.3b | 2.8 ± 0.5c |
高原毛茛 Ranunculus tanguticus | 9.4 ± 0.4a | 8.2 ± 0.8b | 7.7 ± 1.1b | 10.2 ± 0.7a | 8.9 ± 0.8b | 7.8 ± 0.6c |
狼毒 Stellera chamaejasme | 14.3 ± 1.4a | 11.8 ± 1.4b | - | - | - | - |
鹅绒委陵菜 Potentilla anserina | 4.5 ± 0.6a | 3.2 ± 0.5b | 3.1 ± 0.6b | - | - | - |
野草莓 Fragaria vesca | 4.4 ± 0.7a | 4.3 ± 0.8a | 3.5 ± 0.5b | - | - | - |
华西委陵菜 Potentilla potaninii | 3.3 ± 0.5a | 2.5 ± 0.6b | 2.2 ± 0.3b | - | - | - |
高山豆 Tibetia himalaica | 4.1 ± 0.9a | 3.0 ± 0.5b | 2.8 ± 0.5b | - | - | - |
小花草玉梅 Anemone rivularis var. flore-minore | - | - | - | 5.3 ± 0.8a | 4.6 ± 0.6b | 4.4 ± 1.0b |
表2 高海拔(3,600 m)和低海拔(2,900 m)的16个研究种群在套袋、自然授粉和补充授粉处理下的平均花寿命(天, 平均值±标准差), 同行不同字母表示在同一海拔下不同处理之间花寿命差异显著(P < 0.05)。
Table 2 Flower longevity (d, mean ± SD) of 16 research populations when flowers were either excluded from pollinators, open-pollinated or supplemental hand-pollinated at low (2,900 m) and high (3,600 m) elevations. Different letters in the same row at each floral longevity of different treatments indicate significant difference (P < 0.05).
物种 Species | 花寿命 Floral longevity | |||||
---|---|---|---|---|---|---|
低海拔 Low elevation (2,900 m ) | 高海拔 High elevation (3,500 m) | |||||
套袋处理 Pollinator exclusion | 自然授粉 Open pollination | 补充授粉Supplemental pollination | 套袋处理Pollinator exclusion | 自然授粉 Open pollination | 补充授粉Supplemental pollination | |
蒲公英 Taraxacum mongolicum | 5.2 ± 0.8a | 4.7 ± 0.6a | - | 6.5 ± 1.2a | 5.6 ± 0.8b | - |
甘青老鹳草 Geranium pylzowianum | 4.4 ± 0.5a | 3.2 ± 0.4b | 2.8 ± 0.3b | 5.0 ± 0.5a | 3.8 ± 0.3b | 3.0 ± 0.3c |
钝裂银莲花 Anemone obtusiloba | 9.8 ± 0.9a | 8.6 ± 1.1b | 7.6 ± 0.4c | 10.8 ± 0.8a | 9.4 ± 0.7b | 7.5 ± 0.6c |
莓叶委陵菜 Potentilla fragarioides | 3.5 ± 0.4a | 2.8 ± 0.3b | 2.4 ± 0.3c | 3.8 ± 0.4a | 3.3 ± 0.3b | 2.8 ± 0.5c |
高原毛茛 Ranunculus tanguticus | 9.4 ± 0.4a | 8.2 ± 0.8b | 7.7 ± 1.1b | 10.2 ± 0.7a | 8.9 ± 0.8b | 7.8 ± 0.6c |
狼毒 Stellera chamaejasme | 14.3 ± 1.4a | 11.8 ± 1.4b | - | - | - | - |
鹅绒委陵菜 Potentilla anserina | 4.5 ± 0.6a | 3.2 ± 0.5b | 3.1 ± 0.6b | - | - | - |
野草莓 Fragaria vesca | 4.4 ± 0.7a | 4.3 ± 0.8a | 3.5 ± 0.5b | - | - | - |
华西委陵菜 Potentilla potaninii | 3.3 ± 0.5a | 2.5 ± 0.6b | 2.2 ± 0.3b | - | - | - |
高山豆 Tibetia himalaica | 4.1 ± 0.9a | 3.0 ± 0.5b | 2.8 ± 0.5b | - | - | - |
小花草玉梅 Anemone rivularis var. flore-minore | - | - | - | 5.3 ± 0.8a | 4.6 ± 0.6b | 4.4 ± 1.0b |
图1 高海拔(3,600 m)和低海拔(2,900 m) 5种共有物种的平均花寿命(平均值±标准差)的比较。* P < 0.05。
Fig. 1 The mean floral longevity (mean ± SD) of 5 mutual species at low (2,900 m) and high (3,600 m) elevation. * P < 0.05.
图2 群落3和4的研究物种在套袋、补充授粉以及自然授粉处理下的平均花寿命(平均值±标准差)的比较。* P < 0.05。
Fig. 2 The mean flower longevity (mean ± SD) of community 3 & 4 when flowers were either pollinator exclusion, supplemental pollination or open pollination. * P < 0.05.
图3 高海拔和低海拔5种共有物种的花寿命对套袋处理(A)及补充授粉(B)的响应以及花寿命可塑性(C)的变异。
Fig 3 Differences in responses of floral longevity to pollinator exclusion (A) and supplemental pollination (B) and the plasticity of flower longevity (C)in populations of five mutual plant species from low and high elevation.
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