Diversity of Bacillus-like species isolated from potato rhizosphere soils in Yili, Xinjiang

doi:10.17520/biods.2016348

Abstract

Abstract:

This study aims to understand the diversity of Bacillus-like species found in potato rhizosphere soils in Xinjiang, and provide a basis for mining new resources. Bacillus-like bacteria were isolated from potato rhizosphere soils using the cultivable method and preliminarily identified based on the 16S rRNA gene sequences. The spatial distribution pattern of the Bacillus-like bacteria was analyzed using frequency distribution and number. A total of 349 Bacillus-like isolates were obtained from 30 soil samples, and identified as 66 species belonging to 9 genera and 2 families (Bacillaceae and Paenibacillaceae) within Bacilli by 16S rRNA gene sequences. Sixty six selective strains were chosen to be further analyzed based on colony morphology and 16S rRNA gene sequences, and 14 strains were potential novel species based on the lower similarities found between 93.0-98.5% in their closed references type strains. The species numbers of each genus were 41 for Bacillus (41/66, 62.1%), 9 for Paenibacillus (9/66, 13.6%), 6 for Lysinibacillus (6/66, 9.1%), 4 for Pscychrobacillus (4/66, 6.1%), 2 for Fictibacillus (2/66, 3.0%), and 1 for Brevibacillus, Oceanibacillus, Rummelibacillus and Aneurinibacillus, respectively. In the potato rhizosphere soils, the content range of Bacillus-like species were 2.20-8.86×10⁴ cfu/g. The highest colony content and Bacillus-like species, e.g. 8.86×10⁴ cfu/g and 37 species, respectively, occurred in the Mongolian township of Tekes, while the lowest colony content was found in a stud-farm in Nilka County and the lowest Bacillus-like species (9 species) were found in Xigou Village of Fukang City and on the way from Tekes to Zhaosu County. There was large difference in the distribution of Bacillus-like species in the potato rhizosphere soils, but each site was characterized by the same dominant species, Bacillus simplex and Bacillus aryabhattai. Based on their occurrence frequency, the Bacillus-like species could be discriminated as high frequency species including Bacillus simplex, middle frequency species including Bacillus endophyticus, and low frequency species including Bacillus mycoides. Based on the number of Bacillus-like species, 9 collecting sites were divided into two groups, namely high content and richness and low content and richness. Moreover, there was no significant correlation between the distribution of Bacillus-like species and altitude. Thus, there were many Bacillus-like species in potato rhizosphere soils, which provide rich resources for exploring functional strains.

Key words: Bacillus species, potato rhizosphere soil, diversity

Guohong Liu, Bo Liu, Jianmei Che, Qianqian Chen, Naiquan Lin, Weidong Cui. Diversity of Bacillus-like species isolated from potato rhizosphere soils in Yili, Xinjiang[J]. Biodiv Sci, 2017, 25(8): 856-863.

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URL: https://www.biodiversity-science.net/EN/10.17520/biods.2016348

https://www.biodiversity-science.net/EN/Y2017/V25/I8/856

Figures/Tables 4

References 36

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编号 No.	采集地点 Collection site	海拔 Altitude (m)	经纬度 Location
S1	尼勒克县尼勒克镇公社种子队 Seed Team of Nilka Township, Nilka County	1,138	43°46°28? N, 82°35°01? E
S2	阜康市上户沟乡西沟村 Xigou Village, Shanghugou Township, Fukang City	1,261	44°00°17? N, 88°33°46? E
S3	尼勒克县马场 Machang, Nilka County	1,139	43°45°58? N, 82°34°43? E
S4	尼勒克县沙勒漫乡 Shaleman Township, Nilka County	1,110	43°47°08? N, 82°32°15? E
S5	昭苏县农业局试验场 Testing Ground of Agricultural Bureau, Zhaosu County	1,856	44°09'46? N, 81°09°14? E
S6	特克斯蒙古乡(施药) Mongolia Township, Tekes County (pesticide applied)	1,171	43°13°25? N, 81°54°27? E
S7	特克斯蒙古乡 Mongolia Township, Tekes County	1,181	43°13°17? N, 81°52°33? E
S8	特克斯去昭苏路上 On the way from Tekes to Zhaosu County	1,808	-
S9	特克斯县农业技术推广园 Agro Technical Extension Garden, Tekes County	1,181	43°13°17? N, 81°52°33? E

编号 No.	采集地点 Collection site	海拔 Altitude (m)	经纬度 Location
S1	尼勒克县尼勒克镇公社种子队 Seed Team of Nilka Township, Nilka County	1,138	43°46°28? N, 82°35°01? E
S2	阜康市上户沟乡西沟村 Xigou Village, Shanghugou Township, Fukang City	1,261	44°00°17? N, 88°33°46? E
S3	尼勒克县马场 Machang, Nilka County	1,139	43°45°58? N, 82°34°43? E
S4	尼勒克县沙勒漫乡 Shaleman Township, Nilka County	1,110	43°47°08? N, 82°32°15? E
S5	昭苏县农业局试验场 Testing Ground of Agricultural Bureau, Zhaosu County	1,856	44°09'46? N, 81°09°14? E
S6	特克斯蒙古乡(施药) Mongolia Township, Tekes County (pesticide applied)	1,171	43°13°25? N, 81°54°27? E
S7	特克斯蒙古乡 Mongolia Township, Tekes County	1,181	43°13°17? N, 81°52°33? E
S8	特克斯去昭苏路上 On the way from Tekes to Zhaosu County	1,808	-
S9	特克斯县农业技术推广园 Agro Technical Extension Garden, Tekes County	1,181	43°13°17? N, 81°52°33? E

样本编号 Sample no.	芽胞杆菌含量 Bacillus colonies content (×10⁴ cfu/g)	种类数 Species number
S1	3.25	19
S2	5.00	9
S3	2.20	11
S4	6.60	15
S5	3.97	15
S6	7.70	15
S7	8.86	37
S8	4.30	9
S9	3.76	31

样本编号 Sample no.	芽胞杆菌含量 Bacillus colonies content (×10⁴ cfu/g)	种类数 Species number
S1	3.25	19
S2	5.00	9
S3	2.20	11
S4	6.60	15
S5	3.97	15
S6	7.70	15
S7	8.86	37
S8	4.30	9
S9	3.76	31