Biodiv Sci ›› 2017, Vol. 25 ›› Issue (8): 847-855. DOI: 10.17520/biods.2017094
• Original Papers: Animal Diversity • Previous Articles Next Articles
Xiaomeng Zhou1, Shuxin Guo1, Na Song1, Xiumei Zhang1,2,*()
Received:
2017-03-23
Accepted:
2017-06-19
Online:
2017-08-20
Published:
2017-08-31
Contact:
Zhang Xiumei
Xiaomeng Zhou, Shuxin Guo, Na Song, Xiumei Zhang. Identification of Cynoglossus joyneri eggs and larvae by DNA barcoding and morphological method[J]. Biodiv Sci, 2017, 25(8): 847-855.
形态特征 Morphological characters | 短吻红舌鳎 C. joyneri | 长吻红舌鳎 C. lighti | ||||
---|---|---|---|---|---|---|
Li & Wang, 1995 | Zhou & Cheng, 1997 | Nakabo, 2000 | Li & Wang, 1995 | Zhou & Cheng, 1997 | Nakabo, 2000 | |
上、中侧线鳞间鳞数 Scales between upper and middle lateral line | 12-13 | 11-12 | / | 10-11 | 10-11 | / |
体长/体高 Body length/body depth | 3.6-4.4 | 3.8-4.0 | 3.4-4.1 | 3.8-4.5 | 4.2-5.0 | 3.8-4.5 |
头长/头高 Head length/head depth | ≤1 | / | / | >1 | / | / |
Table 1 Comparative counts and distribution of Cynoglossus joyneri and C. lighti in different records
形态特征 Morphological characters | 短吻红舌鳎 C. joyneri | 长吻红舌鳎 C. lighti | ||||
---|---|---|---|---|---|---|
Li & Wang, 1995 | Zhou & Cheng, 1997 | Nakabo, 2000 | Li & Wang, 1995 | Zhou & Cheng, 1997 | Nakabo, 2000 | |
上、中侧线鳞间鳞数 Scales between upper and middle lateral line | 12-13 | 11-12 | / | 10-11 | 10-11 | / |
体长/体高 Body length/body depth | 3.6-4.4 | 3.8-4.0 | 3.4-4.1 | 3.8-4.5 | 4.2-5.0 | 3.8-4.5 |
头长/头高 Head length/head depth | ≤1 | / | / | >1 | / | / |
编号Number | 种类 Species | 序列编号Sequence numbers | 序列来源 Sources of sequences |
---|---|---|---|
1 | 舌鳎属样品 Cynoglossus sp. | egg.1-egg.3; lar.1-lar.3 | 本研究 This study |
2 | 短吻红舌鳎 C. joyneri | joy.1-joy.5 | 本研究(1-3); JQ738430.1; KF979127.1 |
3 | 长吻红舌鳎 C. lighti | lig.1-lig4 | 本研究(1-2); DQ116752.1; HQ711865.1 |
4 | 宽体舌鳎 C. robustus | rob.1-rob.3 | KT718591.1; HM180552.1; HM180553.1 |
5 | 半滑舌鳎 C. semilaevis | sem.1-sem.3 | JN242742.1; JN242743.1; JN242744.1 |
6 | 紫斑舌鳎 C. purpureomaculatus | pur.1-pur.3 | DQ116749.1; JQ738570.1; JQ738571.1 |
7 | 短吻三线舌鳎 C. abbreviatus | abb.1 | DQ116750.1 |
8 | 带纹条鳎 Zebrias zebra | zeb.1-zeb.3 | JQ738566.1; JQ738567.1; JQ738568.1 |
Table 2 Information of Cynoglossus samples and COI gene sequences
编号Number | 种类 Species | 序列编号Sequence numbers | 序列来源 Sources of sequences |
---|---|---|---|
1 | 舌鳎属样品 Cynoglossus sp. | egg.1-egg.3; lar.1-lar.3 | 本研究 This study |
2 | 短吻红舌鳎 C. joyneri | joy.1-joy.5 | 本研究(1-3); JQ738430.1; KF979127.1 |
3 | 长吻红舌鳎 C. lighti | lig.1-lig4 | 本研究(1-2); DQ116752.1; HQ711865.1 |
4 | 宽体舌鳎 C. robustus | rob.1-rob.3 | KT718591.1; HM180552.1; HM180553.1 |
5 | 半滑舌鳎 C. semilaevis | sem.1-sem.3 | JN242742.1; JN242743.1; JN242744.1 |
6 | 紫斑舌鳎 C. purpureomaculatus | pur.1-pur.3 | DQ116749.1; JQ738570.1; JQ738571.1 |
7 | 短吻三线舌鳎 C. abbreviatus | abb.1 | DQ116750.1 |
8 | 带纹条鳎 Zebrias zebra | zeb.1-zeb.3 | JQ738566.1; JQ738567.1; JQ738568.1 |
碱基 Base | 总频率 Total | 密码子第1位点 The first position of codon | 密码子第2位点 The second position of codon | 密码子第3位点 The third position of codon |
---|---|---|---|---|
T | 31.3 | 18.0 | 43.8 | 32.1 |
C | 26.5 | 24.5 | 28.1 | 27.1 |
A | 25.6 | 27.4 | 14.5 | 34.8 |
G | 16.6 | 30.1 | 13.6 | 6.0 |
A+T | 56.9 | 45.4 | 58.3 | 66.9 |
G+C | 43.1 | 54.6 | 41.7 | 33.1 |
Table 3 Average nucleotide frequencies (%) of COI partial sequence of eight species
碱基 Base | 总频率 Total | 密码子第1位点 The first position of codon | 密码子第2位点 The second position of codon | 密码子第3位点 The third position of codon |
---|---|---|---|---|
T | 31.3 | 18.0 | 43.8 | 32.1 |
C | 26.5 | 24.5 | 28.1 | 27.1 |
A | 25.6 | 27.4 | 14.5 | 34.8 |
G | 16.6 | 30.1 | 13.6 | 6.0 |
A+T | 56.9 | 45.4 | 58.3 | 66.9 |
G+C | 43.1 | 54.6 | 41.7 | 33.1 |
种类 Species | 样本量 Sample size | 平均GC含量 Average GC content (%) | |||
---|---|---|---|---|---|
全部位点 Total positions | 第1位点 The first position | 第2位点 The second position | 第3位点 The third position | ||
1舌鳎属样品 Cynoglossus sp. | 6 | 42.9 | 57.2 | 42 | 29.5 |
2短吻红舌鳎 C. joyneri | 5 | 43 | 56.9 | 42 | 29.9 |
3长吻红舌鳎 C. lighti | 4 | 43 | 57.3 | 42 | 29.9 |
4宽体舌鳎 C. robustus | 3 | 42.5 | 53.3 | 42 | 31.9 |
5半滑舌鳎 C. semilaevis | 3 | 40.8 | 52.4 | 42 | 28.2 |
6紫斑舌鳎 C. purpureomaculatus | 3 | 43.1 | 52.3 | 42 | 34.9 |
7短吻三线舌鳎 C. abbreviatus | 1 | 42.9 | 51.8 | 42 | 34.9 |
8带纹条鳎 Zebrias zebra | 3 | 48.4 | 53.9 | 42.5 | 49.0 |
Table 4 The GC content of COI partial sequence for eight species in all codon positions
种类 Species | 样本量 Sample size | 平均GC含量 Average GC content (%) | |||
---|---|---|---|---|---|
全部位点 Total positions | 第1位点 The first position | 第2位点 The second position | 第3位点 The third position | ||
1舌鳎属样品 Cynoglossus sp. | 6 | 42.9 | 57.2 | 42 | 29.5 |
2短吻红舌鳎 C. joyneri | 5 | 43 | 56.9 | 42 | 29.9 |
3长吻红舌鳎 C. lighti | 4 | 43 | 57.3 | 42 | 29.9 |
4宽体舌鳎 C. robustus | 3 | 42.5 | 53.3 | 42 | 31.9 |
5半滑舌鳎 C. semilaevis | 3 | 40.8 | 52.4 | 42 | 28.2 |
6紫斑舌鳎 C. purpureomaculatus | 3 | 43.1 | 52.3 | 42 | 34.9 |
7短吻三线舌鳎 C. abbreviatus | 1 | 42.9 | 51.8 | 42 | 34.9 |
8带纹条鳎 Zebrias zebra | 3 | 48.4 | 53.9 | 42.5 | 49.0 |
种间 Pairwise-species | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 |
---|---|---|---|---|---|---|---|---|
1舌鳎属样品 Cynoglossus sp. | 0.009 | |||||||
2短吻红舌鳎 C. joyneri | 0.009 | 0.008 | ||||||
3长吻红舌鳎 C. lighti | 0.008 | 0.006 | 0.004 | |||||
4宽体舌鳎 C. robustus | 0.236 | 0.240 | 0.240 | 0.002 | ||||
5半滑舌鳎 C. semilaevis | 0.189 | 0.192 | 0.190 | 0.208 | 0.000 | |||
6紫斑舌鳎 C. purpureomaculatus | 0.181 | 0.182 | 0.181 | 0.200 | 0.113 | 0.000 | ||
7短吻三线舌鳎C. abbreviatus | 0.182 | 0.185 | 0.184 | 0.203 | 0.116 | 0.002 | n/c | |
8带纹条鳎 Zebrias zebra | 0.245 | 0.245 | 0.244 | 0.277 | 0.278 | 0.302 | 0.304 | 0.002 |
Table 5 Genetic distance pairwise-species and within-species of eight species
种间 Pairwise-species | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 |
---|---|---|---|---|---|---|---|---|
1舌鳎属样品 Cynoglossus sp. | 0.009 | |||||||
2短吻红舌鳎 C. joyneri | 0.009 | 0.008 | ||||||
3长吻红舌鳎 C. lighti | 0.008 | 0.006 | 0.004 | |||||
4宽体舌鳎 C. robustus | 0.236 | 0.240 | 0.240 | 0.002 | ||||
5半滑舌鳎 C. semilaevis | 0.189 | 0.192 | 0.190 | 0.208 | 0.000 | |||
6紫斑舌鳎 C. purpureomaculatus | 0.181 | 0.182 | 0.181 | 0.200 | 0.113 | 0.000 | ||
7短吻三线舌鳎C. abbreviatus | 0.182 | 0.185 | 0.184 | 0.203 | 0.116 | 0.002 | n/c | |
8带纹条鳎 Zebrias zebra | 0.245 | 0.245 | 0.244 | 0.277 | 0.278 | 0.302 | 0.304 | 0.002 |
Fig. 1 Neighbor-joining tree constructed using the K2P model for COI gene sequences of eight species. The numbers indicate the percentages of bootstrap values (>50%) supporting each node from 1,000 replicates. The Sequence numbers are the same to Table 2.
Fig. 2 Morphological characters of egg and larva of Cynoglossus sp. (a) Embryo ring 1/2 epiboly; (b) 1.16 mm BL newly hatched larva; (c) 6.21 mm BL post-metamorphosis larva.
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