冷箭竹根际土壤中可培养细菌的多样性

doi:10.1360/biodiv.070239

生物多样性 ›› 2008, Vol. 16 ›› Issue (1): 91-95. DOI: 10.1360/biodiv.070239 cstr: 32101.14.biodiv.070239

所属专题：土壤生物与土壤健康

冷箭竹根际土壤中可培养细菌的多样性

刘敏¹^,², 李潞滨², 杨凯³, 韩继刚¹, 朱宝成¹^,⁴^,^*(), 彭镇华²

1 河北大学生命科学学院, 保定 071002
2 中国林业科学研究院林业研究所, 国家林业局林木培育重点实验室, 北京 100091
3 北京农学院农业应用新技术北京市重点实验室, 北京 102206
4 河北农业大学生命科学学院, 保定 071001

收稿日期:2007-09-03 接受日期:2007-11-15 出版日期:2008-01-27 发布日期:2008-01-27
通讯作者: 朱宝成
作者简介:*E-mail:zhu2222@126.com
基金资助:
国家林业局948项目“植物基因测序及功能改良技术引进”(2004-4-60)

Culturable bacterial diversity in rhizosphere ofBashania fangiana

Min Liu¹^,², Lubin Li², Kai Yang³, Jigang Han¹, Baocheng Zhu¹^,⁴^,^*(), Zhenhua Peng²

1 College of Life Science, Hebei University, Baoding 071002
2 Research Institute of Forestry, Chinese Academy of Forestry; Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100091
3 Key Laboratory of New Technology in Agricultural Application in Beijing, Beijing Agricultural College, Beijing 102206
4 College of Life Science, Agricultural University of Hebei, Baoding 071001

Received:2007-09-03 Accepted:2007-11-15 Online:2008-01-27 Published:2008-01-27
Contact: Baocheng Zhu

摘要/Abstract

摘要：

为了解冷箭竹(Bashania fangiana)根际土壤中可培养细菌的多样性, 2006年5月从四川卧龙自然保护区冷箭竹根际土壤中共分离出50株具不同菌落形态的细菌。16S rDNA序列分析结果表明: 50株菌分属于10个属26个种。27株属于变形菌门γ亚群(Gammaproteobacteria)(42.3%)、9株属于厚壁菌门(Firmicutes)(26.9%)、4株属于放线菌门(Actinobacteria) (15.4%)、6株属于变形菌门β亚群(Betaproteobacteria) (7.7%)、3株属于变形菌门α亚群(Alphaproteobacteria) (3.9%), 1株与土地杆菌属(Pedobacter)关系密切。假单胞菌属(Pseudomonas)和芽孢杆菌属(Bacillus)为优势菌属。2株菌为可能的新种或属。研究表明冷箭竹根际土壤中含有较为丰富的微生物多样性。

关键词: Bashania fangiana, 根际细菌, 可培养细菌, 多样性

Abstract

In order to understand the diversity of culturable bacteria, we collected Bashania fangiana rhizosphere soil in Wolong Nature Reserve in May 2006. Then we isolated and cultured 50 bacteria strains with different morphological character of colonies from the soil. Phylogenetic analysis based on the partial 16S rDNA sequences indicated that the 50 strains were clustered into ten genera: 27 strains of Gammaproteobacteria (42.3%), nine strains of Firmicutes (26.9%), four strains of Actinobacteria (15.4%), six strains of Betaproteobacteria (7.7%), three strains of Alphaproteobacteria (3.9%), one strain closely related to the genus Pedobacter.The dominant bacteria were Pseudomonasand Bacillus. Additionally, we found two potential novel species. The result shows that the bacterial population diversity is abundant in B. fangiana rhizosphere soil.

Key words: Bashania fangiana, rhizosphere bacteria, culturable bacteria, diversity

刘敏, 李潞滨, 杨凯, 韩继刚, 朱宝成, 彭镇华 (2008) 冷箭竹根际土壤中可培养细菌的多样性. 生物多样性, 16, 91-95. DOI: 10.1360/biodiv.070239.

Min Liu, Lubin Li, Kai Yang, Jigang Han, Baocheng Zhu, Zhenhua Peng (2008) Culturable bacterial diversity in rhizosphere ofBashania fangiana. Biodiversity Science, 16, 91-95. DOI: 10.1360/biodiv.070239.

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链接本文: https://www.biodiversity-science.net/CN/10.1360/biodiv.070239

https://www.biodiversity-science.net/CN/Y2008/V16/I1/91

图/表 2

参考文献 21

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类群 Genus	代表菌株(登录号) Representative isolate (accession no.)	菌株数 No. of isolates in OTU*	最相近菌种(登录号) Nearest type strain (accession no.)	序列相似性 Similarity (%)
假单胞菌属 Pseudomonas	B34(EU169189)	7	Pseudomonas tolaasii (AF320989)	98
	B46(EU169146)	3	P. brenneri (AF268968)	98
	B64(EU169192)	1	P. poae (AJ492829)	97
	B61(EU169159)	1	P. trivialis (DQ885949)	99
	B62(EU169158)	1	P. frederiksbergensis (AY785733)	99
	B25(EU169168)	1	P. lini (AY035996)	99
	B23(EU169169)	1	P. aurantiaca (AY271792)	99
	B69(EU169163)	8	P. fluorescens (AF094730)	99
	B5(EU169177)	1	P. grimontii (AF268029)	99
不动杆菌属 Acinetobacter	B38(EU169148)	1	Acinetobacter johnsonii (DQ257425)	95
不动杆菌属 Acinetobacter	B33(EU169147)	2	A. johnsonii (DQ257425)	98
贪铜菌属 Cupriavidus	B47(EU169175)	1	Cupriavidus basilensis (AY860224)	99
贪噬菌属 Variovorax	B54(EU169184)	5	Variovorax paradoxus (DQ241396)	99
叶杆菌属 Phyllobacterium	B70(EU169181)	3	Phyllobacterium myrsinacearum (AJ011330)	98
土地杆菌属 Pedobacter	B52(EU169155)	1	Pedobacter cryoconitis (AJ438170)	99
微杆菌属 Microbacterium	B26(EU169182)	1	Microbacterium trichotecenolyticum(AB167383)	99
微杆菌属 Microbacterium	B9(EU169183)	1	M. schleiferi (Y17237)	91
壤球菌属 Agrococcus	B16(EU169180)	1	Agrococcus jenensis (AM410679)	99
微球菌属 Micrococcus	B7(EU169174)	1	Micrococcus indicus (AM158920)	99
芽孢杆菌属 Bacillus	B1(EU169187)	1	Bacillus mycoides (EF210295)	99
	B31(EU169186)	3	B. weihenstephanensis (AM062685)	99
	B72(EU169167)	1	B. cereus (AB244464)	100
	B15(EU169176)	1	B. megaterium (AB271751)	100
	B22(EU169170)	1	B. badius (AY803745)	99
	B43(EU169188)	1	B. subtilis (EF656456)	99
	B53(EU169154)	1	B. flexus (EF157301)	99

类群 Genus	代表菌株(登录号) Representative isolate (accession no.)	菌株数 No. of isolates in OTU*	最相近菌种(登录号) Nearest type strain (accession no.)	序列相似性 Similarity (%)
假单胞菌属 Pseudomonas	B34(EU169189)	7	Pseudomonas tolaasii (AF320989)	98
	B46(EU169146)	3	P. brenneri (AF268968)	98
	B64(EU169192)	1	P. poae (AJ492829)	97
	B61(EU169159)	1	P. trivialis (DQ885949)	99
	B62(EU169158)	1	P. frederiksbergensis (AY785733)	99
	B25(EU169168)	1	P. lini (AY035996)	99
	B23(EU169169)	1	P. aurantiaca (AY271792)	99
	B69(EU169163)	8	P. fluorescens (AF094730)	99
	B5(EU169177)	1	P. grimontii (AF268029)	99
不动杆菌属 Acinetobacter	B38(EU169148)	1	Acinetobacter johnsonii (DQ257425)	95
不动杆菌属 Acinetobacter	B33(EU169147)	2	A. johnsonii (DQ257425)	98
贪铜菌属 Cupriavidus	B47(EU169175)	1	Cupriavidus basilensis (AY860224)	99
贪噬菌属 Variovorax	B54(EU169184)	5	Variovorax paradoxus (DQ241396)	99
叶杆菌属 Phyllobacterium	B70(EU169181)	3	Phyllobacterium myrsinacearum (AJ011330)	98
土地杆菌属 Pedobacter	B52(EU169155)	1	Pedobacter cryoconitis (AJ438170)	99
微杆菌属 Microbacterium	B26(EU169182)	1	Microbacterium trichotecenolyticum(AB167383)	99
微杆菌属 Microbacterium	B9(EU169183)	1	M. schleiferi (Y17237)	91
壤球菌属 Agrococcus	B16(EU169180)	1	Agrococcus jenensis (AM410679)	99
微球菌属 Micrococcus	B7(EU169174)	1	Micrococcus indicus (AM158920)	99
芽孢杆菌属 Bacillus	B1(EU169187)	1	Bacillus mycoides (EF210295)	99
	B31(EU169186)	3	B. weihenstephanensis (AM062685)	99
	B72(EU169167)	1	B. cereus (AB244464)	100
	B15(EU169176)	1	B. megaterium (AB271751)	100
	B22(EU169170)	1	B. badius (AY803745)	99
	B43(EU169188)	1	B. subtilis (EF656456)	99
	B53(EU169154)	1	B. flexus (EF157301)	99

冷箭竹根际土壤中可培养细菌的多样性

Culturable bacterial diversity in rhizosphere ofBashania fangiana

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