生物多样性 ›› 2017, Vol. 25 ›› Issue (4): 409-417.DOI: 10.17520/biods.2016331

• 研究报告: 动物多样性 • 上一篇    下一篇

白洋淀湿地蝗虫多样性调查及DNA条形码应用研究

郝金凤, 张晓红, 王昱淞, 刘金林, 智永超, 李新江*()   

  1. 河北大学生命科学学院河北省动物系统学与应用重点实验室, 河北保定 071002
  • 收稿日期:2016-11-22 接受日期:2017-04-10 出版日期:2017-04-20 发布日期:2017-04-20
  • 通讯作者: 李新江
  • 基金资助:
    基金项目: 河北省高等学校科学技术研究项目(QN2014017)

Diversity investigation and application of DNA barcoding of Acridoidea from Baiyangdian Wetland

Jinfeng Hao, Xiaohong Zhang, Yusong Wang, Jinlin Liu, Yongchao Zhi, Xinjiang Li*()   

  1. The Key Laboratory of Zoological Systematics and Application, College of Life Sciences, Hebei University, Baoding, Hebei 071002
  • Received:2016-11-22 Accepted:2017-04-10 Online:2017-04-20 Published:2017-04-20
  • Contact: Li Xinjiang

摘要:

为了解白洋淀湿地蝗虫的物种多样性及其分布情况, 探究DNA条形码技术在物种鉴定方面的可行性, 本研究对白洋淀湿地的蝗虫进行了采集调查。采用自行设计的cox1基因引物, 本研究共扩增得到21种蝗虫的cox1序列97条, 并利用构建NJ系统发育树法、计算遗传距离的ABGD法以及序列差异阈值分类的MOTU法对已得到的序列连同GenBank下载的10种25条cox1序列进行了比较分析。结果表明: 白洋淀湿地蝗总科昆虫共有6科23属34种, 包括1个新记录属, 即异爪蝗属(Euchorthippus), 和3个新记录种, 即素色异爪蝗(E. unicolor)、柳枝负蝗(Atractomorpha psittacina)和日本稻蝗(Oxya japonica); 蝗虫在白洋淀湿地主要分布在农田及其周围、堤坝及其周围以及淀边草丛; DNA条形码技术能够在种级阶元对蝗总科物种进行良好地解析, 但物种鉴定时, 不能完全脱离形态学方法。

关键词: 直翅目, 蝗总科, 白洋淀湿地, 多样性, DNA条形码

Abstract:

Both the species diversity and distribution pattern of the superfamily Acridoidea of the suborder Caelifera have important contributions to understanding the local biodiversity of the Baiyangdian Wetland. This research tries to study the species diversity and distribution pattern of the superfamily Acridoidea within the Baiyangdian Wetland and test the feasibility of DNA barcoding in species identification for this superfamily. Sequences of the cox1 gene were obtained from 97 individuals of 21 species of the superfamily Acridoidea. Phylogenetic, genetic distance and sequence difference threshold analyses using the Neighbor Joining (NJ), Automatic Barcode Gap Discovery (ABGD) and Molecular Defined Operational Taxonomic Units (MOTU) methods, respectively, were performed for these and the 25 additional sequences of 10 species downloaded from GenBank. The results indicate that there are 34 species, 23 genera, and 6 families of the superfamily Acridoidea insects around the farmland, dam, and grassland of the Baiyangdian Wetland, including a new-record genus, Euchorthippus, and three new-record species, Euchorthippus unicolor, Atractomorpha psittacina and Oxya japonica. The DNA barcoding technology therefore is very efficient and helpful for identifying the species of the superfamily Acridoidea, although the morphological approach is still playing a key role in the species identifications.

Key words: Orthoptera, Acridoidea, Baiyangdian Wetland, diversity, DNA barcoding