西北部分地区苦马豆根瘤菌的遗传多样性

doi:10.3724/SP.J.1003.2009.08218

生物多样性 ›› 2009, Vol. 17 ›› Issue (1): 69-75. DOI: 10.3724/SP.J.1003.2009.08218 cstr: 32101.14.SP.J.1003.2009.08218

所属专题：土壤生物与土壤健康

西北部分地区苦马豆根瘤菌的遗传多样性

徐琳¹^,², 徐佳洁¹, 刘巧莉¹, 谢瑞美¹, 韦革宏¹^,^*()

1 西北农林科技大学生命科学学院, 陕西杨凌 712100
2 河西学院生命科学与工程系, 甘肃张掖 734000

收稿日期:2008-08-28 接受日期:2008-12-14 出版日期:2009-01-20 发布日期:2009-01-20
通讯作者: 韦革宏
作者简介:* E-mail: weigehong@yahoo.com.cn
基金资助:
国家自然科学基金(30670372);科技部农业微生物菌种资源整理、整合及共享试点子项目(2005DKA21201)

Genetic diversity in rhizobia isolated from Sphaerophysa salsula in several regions of northwestern China

Lin Xu¹^,², Jiajie Xu¹, Qiaoli Liu¹, Ruimei Xie¹, Gehong Wei¹^,^*()

1 College of Life Sciences, Northwest A & F University, Yangling, Shaanxi 712100
2 Department of Life Science and Engeneering, Hexi University, Zhangye, Gansu 734000

Received:2008-08-28 Accepted:2008-12-14 Online:2009-01-20 Published:2009-01-20
Contact: Gehong Wei

摘要/Abstract

摘要：

苦马豆(Sphaerophysa salsula)是荒漠区重要的豆科植物。为了研究其共生根瘤菌的多样性, 本试验采用16S rDNA PCR-RFLP和16S rDNA全序列分析方法, 对西北部分地区的苦马豆根瘤菌进行了遗传多样性及系统发育分析。结果表明, 57株供试菌株共产生了9种遗传图谱类型, 对每种图谱类型的代表性菌株进行16S rDNA全序列分析的结果表明, 它们分别归属于中慢生根瘤菌属(Mesorhizobium)、根瘤菌属(Rhizobium)、中华根瘤菌属(Sinorhizobium)、土壤杆菌属(Agrobacterium)、叶杆菌属(Phyllobacterium)和Shinella kummerowiae。不同地域的菌株在多样性方面也有明显差异: 分离自银川的苦马豆根瘤菌的Jaccard相似性系数较低; 而来自民乐县和临泽县的菌株有着非常丰富的遗传多样性, 其Simpson指数分别为0.826和0.710, Shannon-Wiener指数分别为1.831和1.530。以上结果为进一步确定西北地区豆科植物根瘤菌的系统分类地位提供了依据。

关键词: Sphaerophysa salsula, 系统发育, 16S rDNA PCR-RFLP, 16S rDNA全序列, 遗传多样性

Abstract

Sphaerophysa salsula is a highly nutritive and drought-tolerant perennial grass distributed in mid-Asia and northwestern China. This legume plant is highly prized for the revegetation of drought and high alkali soils. Our objective was to better understand the diversity and phylogeny of rhizobia collected from nodules of Sphaerophsa salsula in different regions of northwestern China. We estimated genetic diversity using 16S rDNA PCR-RFLP and 16S rDNA sequencing. Nine genotypes were tested from 57 strains. 16S rDNA sequencing revealed that these rhizobial strains belonged to the following genera: Mesorhizobium, Rhizobium, Sinorhizobium, Agrobacterium, Phyllobacterium and Shinella kummerowiae. The Jaccard similarity coefficient of the strains from Yinchuan was low while the strains isolated from Minle and Linze had rich diversity, with Simpson indices of 0.826 and 0.710, respectively, and Shannon-Wiener indices of 1.831 and 1.530, respectively. This study provides basic information for the phylogeny of rhizobia in northwestern China.

Key words: Sphaerophysa salsula, phylogeny, 16S rDNA PCR-RFLP, 16S rDNA sequencing, genetic diversity

徐琳, 徐佳洁, 刘巧莉, 谢瑞美, 韦革宏 (2009) 西北部分地区苦马豆根瘤菌的遗传多样性. 生物多样性, 17, 69-75. DOI: 10.3724/SP.J.1003.2009.08218.

Lin Xu, Jiajie Xu, Qiaoli Liu, Ruimei Xie, Gehong Wei (2009) Genetic diversity in rhizobia isolated from Sphaerophysa salsula in several regions of northwestern China. Biodiversity Science, 17, 69-75. DOI: 10.3724/SP.J.1003.2009.08218.

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https://www.biodiversity-science.net/CN/Y2009/V17/I1/69

图/表 4

参考文献 22

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酶切类型 Fingerprint pattern	分离地 Location
酶切类型 Fingerprint pattern	甘肃民乐县 Minle County, Gansu	甘肃张掖市 Zhangye City, Gansu	甘肃临泽县 Linze County, Gansu	甘肃玉门市 Yumen City, Gansu	宁夏银川市 Yinchuan City, Ningxia	新疆伊犁市 Yili City, Xinjiang
AAB	GS0181, GS0185, GS0188, GS0192, GS0198	GS0215	GS0240
BBD	GS0184		GS0218	GS0237
CCB	GS0170, GS0171 GS0168		GS0219, GS0223, GS0227, GS0230, GS0231, GS0232		NX0171, NX0172, NX0173	XJ0216
CEB	GS0176, GS0196, GS0199, GS0200, GS0201, GS0204	GS0206, GS0209 GS0210, GS0214	GS0216, GS0233 GS0234
DDB			GS0228
DFB	GS0172, GS0202
EDB	GS0177, GS0178, GS0187			GS0238
EDC	GS0166, GS0169, GS0173, GS0180	GS0207, GS0211, GS0212, GS0213	GS0220			XJ0217, XJ0218, XJ0219, XJ0221
FDC						XJ0229

酶切类型 Fingerprint pattern	分离地 Location
酶切类型 Fingerprint pattern	甘肃民乐县 Minle County, Gansu	甘肃张掖市 Zhangye City, Gansu	甘肃临泽县 Linze County, Gansu	甘肃玉门市 Yumen City, Gansu	宁夏银川市 Yinchuan City, Ningxia	新疆伊犁市 Yili City, Xinjiang
AAB	GS0181, GS0185, GS0188, GS0192, GS0198	GS0215	GS0240
BBD	GS0184		GS0218	GS0237
CCB	GS0170, GS0171 GS0168		GS0219, GS0223, GS0227, GS0230, GS0231, GS0232		NX0171, NX0172, NX0173	XJ0216
CEB	GS0176, GS0196, GS0199, GS0200, GS0201, GS0204	GS0206, GS0209 GS0210, GS0214	GS0216, GS0233 GS0234
DDB			GS0228
DFB	GS0172, GS0202
EDB	GS0177, GS0178, GS0187			GS0238
EDC	GS0166, GS0169, GS0173, GS0180	GS0207, GS0211, GS0212, GS0213	GS0220			XJ0217, XJ0218, XJ0219, XJ0221
FDC						XJ0229

来源 Location	Jaccard相似性系数 Jaccard similarity coefficient					Simpson 指数 Simpson index	Shannon-Wiener指数 Shannon-Wiener index
来源 Location	甘肃张掖市 Zhangye City, Gansu	甘肃临泽县 Linze County, Gansu	甘肃玉门市 Yumen City, Gansu	宁夏银川市 Yinchuan City, Ningxia	新疆伊犁市 Yili City, Xinjiang	Simpson 指数 Simpson index	Shannon-Wiener指数 Shannon-Wiener index
甘肃张掖市 Zhangye City, Gansu						0.593	0. 965
甘肃临泽县 Linze County, Gansu	0.50					0.710	1.530
甘肃玉门市 Yumen City, Gansu	0.25	0.14				0.500	0.693
宁夏银川市 Yinchuan City, Ningxia	0.00	0.17	0.00			0.000	0.000
新疆伊犁市 Yili City, Xinjiang	0.20	0.30	0.00	0.33		0.500	0.868
甘肃民乐县 Minle County, Gansu	0.38	0.56	0.25	0.13	0.22	0.826	1.831

来源 Location	Jaccard相似性系数 Jaccard similarity coefficient					Simpson 指数 Simpson index	Shannon-Wiener指数 Shannon-Wiener index
来源 Location	甘肃张掖市 Zhangye City, Gansu	甘肃临泽县 Linze County, Gansu	甘肃玉门市 Yumen City, Gansu	宁夏银川市 Yinchuan City, Ningxia	新疆伊犁市 Yili City, Xinjiang	Simpson 指数 Simpson index	Shannon-Wiener指数 Shannon-Wiener index
甘肃张掖市 Zhangye City, Gansu						0.593	0. 965
甘肃临泽县 Linze County, Gansu	0.50					0.710	1.530
甘肃玉门市 Yumen City, Gansu	0.25	0.14				0.500	0.693
宁夏银川市 Yinchuan City, Ningxia	0.00	0.17	0.00			0.000	0.000
新疆伊犁市 Yili City, Xinjiang	0.20	0.30	0.00	0.33		0.500	0.868
甘肃民乐县 Minle County, Gansu	0.38	0.56	0.25	0.13	0.22	0.826	1.831

西北部分地区苦马豆根瘤菌的遗传多样性

Genetic diversity in rhizobia isolated from Sphaerophysa salsula in several regions of northwestern China

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