观光木片断化居群的遗传多样性和交配系统

doi:10.3724/SP.J.1003.2012.09083

生物多样性 ›› 2012, Vol. 20 ›› Issue (6): 676-684. DOI: 10.3724/SP.J.1003.2012.09083 cstr: 32101.14.SP.J.1003.2012.09083

所属专题：传粉生物学

观光木片断化居群的遗传多样性和交配系统

王霞¹^,², 王静¹, 蒋敬虎¹^,², 康明¹^,^*()

1 中国科学院华南植物园, 中国科学院植物资源保护与可持续利用重点实验室, 广州 510650
2 中国科学院大学, 北京 100049

收稿日期:2012-03-27 接受日期:2012-06-25 出版日期:2012-11-20 发布日期:2013-01-04
通讯作者: 康明
作者简介:* E-mail: mingkang@scbg.ac.cn
基金资助:
中国科学院知识创新工程重要方向性项目(KSCX2-YW-Z-1024);国家自然科学基金(31000148)

Genetic diversity and the mating system in a fragmented population of Tsoongiodendron odorum

Xia Wang¹^,², Jing Wang¹, Jinghu Jiang¹^,², Ming Kang¹^,^*()

1 Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650
2 University of the Chinese Academy of Sciences, Beijing 100049

Received:2012-03-27 Accepted:2012-06-25 Online:2012-11-20 Published:2013-01-04
Contact: Ming Kang

摘要/Abstract

摘要：

观光木(Tsoongiodendron odorum)是我国特有的濒危植物, 有居群较小、间断分布的特点。为探讨生境片断化对观光木居群的遗传多样性和交配系统的影响, 我们采用8对微卫星引物对采自广东南昆山的观光木片断化居群的61个成株和15个家系共780个种子进行了基因分型, 调查了5个空间隔离的斑块中观光木的两代遗传多样性以及各个层次(居群、斑块及个体)的交配系统参数。结果显示: 片断化生境中观光木成株遗传多样性水平适中(H_E= 0.522), 种子遗传多样性比成株稍低(H_E= 0.499), 但没有显著的差异, 近交系数在种子中也没有显著的升高, 暗示生境片断化并没有侵蚀观光木的遗传多样性; 多位点交配系统分析(MLTR)结果表明, 该片断化生境中观光木为高度异交树种(t_m = 1.000), 只有较少的双亲近交(biparental inbreeding)和相关性交配(correlated mating)事件发生, 但有效花粉供体(effective pollen donor)数目较少(N_ep为3.7-5.4); 5个斑块间异交率差异不明显, 但小的斑块有效花粉供体相对较多; 另外, 观光木个体之间的异交率存在明显差异, 少数个体存在自交现象。这些结果为濒危物种观光木的长期保护提供了重要的遗传学信息。

关键词: Tsoongiodendron odorum, 生境片断化, 多位点交配系统分析, 保育遗传学, 微卫星

Abstract

Habitat fragmentation is one of the most serious threats to plant diversity. In general, fragmentation negatively impacts the genetic variability of plant populations due to increased random genetic drift, inbreeding, and reductions in gene flow. To investigate the effect of habitat fragmentation on genetic diversity and the mating system of Tsoongiodendron odorum, in this study, we analyzed genetic diversity and the mating system in hierarchical levels at the population, stands, and the individual scales in a fragmented T. odorum population. We sampled and mapped 61 adult individuals from the population. Using eight microsatellite loci, we genotyped a total of 780 seeds from 15 maternal trees for the mating system analysis. The results revealed moderate levels of genetic diversity in both adults (H_E= 0.522) and seeds (H_E= 0.499) with no significant differences between the two ontogenic stages. In addition, we did not observe a significant increase in the seeds inbreeding coefficient. Results from the multilocus mating system analysis indicated that T. odorum was an outbreeding species with a multilocus outcrossing rate (t_m) of 1.000. A small number of biparental inbreeding and correlated mating events were detected in this fragmented population. We found a small number of effective pollen donors (N_ep is between 3.7 and 5.4), which seems to be a common character of insect-pollinated canopy trees. Minor differences in outcrossing rates were detected among stands, and more pollen donors were found in smaller stands. However, outcrossing rate was significantly different among individuals, and a few selfing events were detected in some seed trees. These results may provide fundamental information required to establish long term conservation strategies for this endangered tree which is endemic to China.

Key words: Tsoongiodendron odorum, habitat fragmentation, multilocus mating system analysis, conservation genetics, microsatellite

王霞, 王静, 蒋敬虎, 康明 (2012) 观光木片断化居群的遗传多样性和交配系统. 生物多样性, 20, 676-684. DOI: 10.3724/SP.J.1003.2012.09083.

Xia Wang, Jing Wang, Jinghu Jiang, Ming Kang (2012) Genetic diversity and the mating system in a fragmented population of Tsoongiodendron odorum. Biodiversity Science, 20, 676-684. DOI: 10.3724/SP.J.1003.2012.09083.

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链接本文: https://www.biodiversity-science.net/CN/10.3724/SP.J.1003.2012.09083

https://www.biodiversity-science.net/CN/Y2012/V20/I6/676

图/表 7

参考文献 39

[1]	Aguilar R, Ashworth L, Galetto L, Aizen MA (2006) Plant reproductive susceptibility to habitat fragmentation: review and synthesis through a meta-analysis. Ecology Letters, 9, 968-980. URL PMID
[2]	Aguilar R, Quesada M, Ashworth L, Herrerias-Diego Y, Lobo J (2008) Genetic consequences of habitat fragmentation in plant populations: susceptible signals in plant traits and methodological approaches. Molecular Ecology, 17, 5177-5188. DOI URL PMID
[3]	Debout GDG, Doucet JL, Hardy OJ (2011) Population history and gene dispersal inferred from spatial genetic structure of a Central African timber tree, Distemonanthus benthamianus (Caesalpinioideae). Heredity, 106, 88-99.
[4]	De-Lucas AI, Robledo-Arnuncio JJ, Hidalgo E, González-Martínez SC (2008) Mating system and pollen gene flow in Mediterranean maritime pine. Heredity, 100, 390-399. DOI URL PMID
[5]	Dick CW, Etchelecu G, Austerlitz F (2003) Pollen dispersal of tropical trees (Dinizia excelsa: Fabaceae) by native insects and African honeybees in pristine and fragmented Amazonian rainforest. Molecular Ecology, 12, 753-764. URL PMID
[6]	Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin, 19, 11-15.
[7]	Eckert CG, Kalisz S, Geber MA, Sargent R, Elle E, Cheptou PO, Goodwillie C, Johnston MO, Kelly JK, Moeller DA, Porcher E, Ree RH, Vallejo-Marín M, Winn AA (2010) Plant mating systems in a changing world. Trends in Ecology and Evolution, 25, 35-43.
[8]	El Mousadik A, Petit RJ (1996) High level of genetic differentiation for allelic richness among populations of the argan tree [Argania spinosa (L.) Skeels] endemic to Morocco. Theoretical and Applied Genetics, 92, 832-839.
[9]	Fu L (付琳), Xu FX (徐凤霞), Zeng QW (曾庆文) (2009) Studies on microsporogenesis and development of male gametophyte in Tsoongiodendron odorum Chun. Journal of Tropical and Subtropical Botany (热带亚热带植物学报), 17, 419-426. (in Chinese with English abstract)
[10]	Fu LK (傅立国), Jin JM (金鉴明) (1992) China Plant Red Data Book: Rare and Endangered Plants (中国植物红皮书: 稀有濒危植物). Science Press, Beijing. (in Chinese)
[11]	Goudet J (2001) FSTAT, a program to estimate and test gene diversities and fixation indices (Version 2. 9. 3). http://www.unil.ch/izea/softwares/fstat.html.
[12]	He RK, Wang J, Huang HW (2012) Long-distance gene dispersal inferred from spatial genetic structure in Handeliodendron bodinieri, an endangered tree from karst forest in southwest China. Biochemical Systematics and Ecology, 44, 295-302.
[13]	Huang JX (黄久香), Zhuang XY (庄雪影) (2002) Genetic diversity of the populations of Tsoongiodendron odorum. Acta Phytoecologica Sinica (植物生态学报), 26, 413-419. (in Chinese with English abstract)
[14]	Hufford KM, Hamrick JL (2003) Viability selection at three early life stages of the tropical tree, Platypodium elegans (Fabaceae, Papilionoideae). Evolution, 57, 518-526.
[15]	Ishida K (2008) Effects of inbreeding on the magnitude of inbreeding depression in a highly self-fertilizing tree, Magnolia obovata. Ecology Resources, 23, 995-1003.
[16]	Jiang JH, Wang J, Kang M, Sun W, Huang HW (2011) Isolation and characterization of microsatellite loci in Tsoongiodendron odorum (Magnoliaceae). American Jour- nal of Botany, 98, 284-286.
[17]	Kang M, Jiang MX, Huang HW (2005) Genetic diversity in fragmented populations of Berchemiella wilsonii var. pubipetiolata (Rhamnaceae). Annals of Botany, 95, 1145-1151.
[18]	Kikuchi S, Isagi Y (2002) Microsatellite genetic variation in small and isolated populations of Magnolia sieboldii ssp. japonica. Heredity, 88, 313-321. DOI URL PMID
[19]	Liu YH (刘玉壶) (1984) A preliminary study on the taxonomy of the family Magnoliaceae. Acta Phytotaxonomica Sinica (植物分类学报), 22, 89-109. (in Chinese with English abstract)
[20]	Luijten SH, Dierick A, Oostermeijer JGB, Raijmann LEL, Den Nijs HCM (2000) Population size, genetic variation, and reproductive success in a rapidly declining, self-incom- patible perennial (Arnica montana) in the Netherlands. Conservation Biology, 14, 1776-1787.
[21]	Mathiasen P, Rovere AE, Premoli AC (2007) Genetic structure and early effects of inbreeding in fragmented temperate forests of a self-incompatible tree, Embothrium coccineum. Conservation Biology, 21, 232-240.
[22]	Nybom H (2004) Comparison of different nuclear DNA markers for estimating intraspecific genetic diversity in plants. Molecular Ecology, 13, 1143-1155.
[23]	Patterson B, Vaillancourt RE, Pilbeam DJ, Potts BM (2004) Factors affecting variation in outcrossing rate in Eucalyptus globulus. Australian Journal of Botany, 52, 773-780.
[24]	Rasmussen KK, Kollmann J (2008) Low genetic diversity in small peripheral populations of a rare European tree (Sorbus torminalis) dominated by clonal reproduction. Conservation Genetics, 9, 1533-1539.
[25]	Ritland K (1989) Correlated matings in the partial selfer Mimulus guttatus. Evolution, 43, 848-859.
[26]	Ritland K (2002) Extensions of models for the estimation of mating systems using n independent loci. Heredity, 88, 221-228.
[27]	Rousset F (2008) GENEPOP'007: a complete re-implementa- tion of the GENEPOP software for Windows and Linux. Molecular Ecology Resources, 8, 103-106.
[28]	Sampson JF (1998) Multiple paternity in Eucalyptus rameliana (Myrtaceae). Heredity, 81, 349-355.
[29]	Schemske DW, Husband BC, Ruckelhaus MH, Goodwille C, Parker IM, Bishop JG (1994) Evaluating approaches to the conservation of rare and endangered plants. Ecology, 75, 584-606.
[30]	Silva CRS, Albuquerque PSB, Ervedosa FR, Mota JWS, Figueira A, Sebbenn AM (2011) Understanding the genetic diversity, spatial genetic structure and mating system at the hierarchical levels of fruits and individuals of a continuous Theobroma cacao population from the Brazilian Amazon. Heredity, 106, 973-985.
[31]	Sork VL, Smouse PE (2006) Genetic analysis of landscape connectivity in tree populations. Landscape Ecology, 21, 821-836.
[32]	Stoeckel S, Grange J, Fernandez-Manjarres JF, Bilger I, Frascaria-Lacoste N, Mariette S (2006) Heterozygote excess in a self-incompatible and partially clonal forest tree species―Prunus avium L. Molecular Ecology, 15, 2109-2118.
[33]	Sun M, Ritland K (1998) Mating system of yellow starthistle (Centaurea solstitialis), a successful colonizer in North America. Heredity, 80, 225-232.
[34]	Tamaki I, Setsuko S, Tomaru N (2009) Estimation of outcrossing rates at hierarchical levels of fruits, individuals, populations and species in Magnolia stellata. Heredity, 102, 381-388.
[35]	Wang R, Ai B, Gao BQ, Yu S, Li YY, Chen XY (2009) Spatial genetic structure and restricted gene flow in a functionally dioecious fig, Ficus pumila L. var. pumila (Moraceae). Population Ecology, 51, 307-315.
[36]	White GM, Boshier DH, Powel W (2002) Increased pollen flow counteracts fragmentation in a tropical dry forest: an example from Swietenia humilis Zuccarini. Proceedings of the National Academy of Sciences, USA, 99, 2038-2042.
[37]	Xu H (许涵), Huang JX (黄久香), Zhang H (张浩), Peng YS (彭逸生), He ZJ (何仲坚) (2007) Population structure and distribution pattern of Tsoongiodendron odorum on Nankunshan, Guangdong Province. Journal of South China Agricultural University (华南农业大学学报), 28, 73-77. (in Chinese with English abstract)
[38]	Zhang DY (张大勇), Jiang XH (姜新华) (2001) Mating system evolution, resource allocation, and genetic diversity in plants. Acta Phytoecologica Sinica (植物生态学报), 25, 130-143. (in Chinese with English abstract)
[39]	Zeng QW (曾庆文), Gao ZZ (高泽正), Xing FW (邢福武), Zhang DX (张奠湘), Gong X (龚洵) (2004) Pollination biology of an endangered species: Tsoongiodendron odorum (Magnoliaceae). In: Proceedings of the Fifth National Symposium on the Conservation and Sustainable Use of Biodiversity in China (第5届生物多样性保护和持续利用研讨会论文集) (ed. Biodiversity Committee, the Chinese Academy of Sciences), pp. 199-204. China Meteorological Press, Beijing. (in Chinese with English abstract)

斑块 Stand	成株数目 No. of adults	采种成株 No. of seed trees	果实数目 No. of fruits	种子数目 No. of seeds
NKS1	5	1	4	60
NKS2	13	3	9	134
NKS3	20	7	26	389
NKS4	16	3	11	168
NKS5	7	1	2	29
总计 Total	61	15	52	780

斑块 Stand	成株数目 No. of adults	采种成株 No. of seed trees	果实数目 No. of fruits	种子数目 No. of seeds
NKS1	5	1	4	60
NKS2	13	3	9	134
NKS3	20	7	26	389
NKS4	16	3	11	168
NKS5	7	1	2	29
总计 Total	61	15	52	780

引物 Primer	成株 Adults					种子 Seeds					总计 Total
引物 Primer	NKS1	NKS2	NKS3	NKS4	NKS5	NKS1	NKS2	NKS3	NKS4	NKS5	总计 Total
TOD1	1	3	3	3	2	2	3	3	3	1	3
TOD2	2	3	3	4	3	3	3	4	4	3	4
TOD5	3	6	6	9	5	5	7	7	6	4	9
TOD6	4	5	6	5	4	4	5	6	5	4	6
TOD8	2	1	2	3	2	1	3	3	2	1	3
TOD11	2	2	2	3	3	2	3	2	3	2	3
TOD12	2	2	3	3	3	3	4	3	3	2	5
TOD52	4	6	9	9	5	9	7	10	8	2	15
总计 Total	20	28	34	39	27	29	35	38	34	19	48

引物 Primer	成株 Adults					种子 Seeds					总计 Total
引物 Primer	NKS1	NKS2	NKS3	NKS4	NKS5	NKS1	NKS2	NKS3	NKS4	NKS5	总计 Total
TOD1	1	3	3	3	2	2	3	3	3	1	3
TOD2	2	3	3	4	3	3	3	4	4	3	4
TOD5	3	6	6	9	5	5	7	7	6	4	9
TOD6	4	5	6	5	4	4	5	6	5	4	6
TOD8	2	1	2	3	2	1	3	3	2	1	3
TOD11	2	2	2	3	3	2	3	2	3	2	3
TOD12	2	2	3	3	3	3	4	3	3	2	5
TOD52	4	6	9	9	5	9	7	10	8	2	15
总计 Total	20	28	34	39	27	29	35	38	34	19	48

生活史 Stage	斑块 Stands	样本 Sample size (N)	等位基因数 No. of alleles per locus (A)	等位基因丰富度 Allelic richness per locus (A_R)	观测杂合度 Observed heterozygosity (H_O)	预期杂合度 Expected heterozygosity (H_E)	近交系数 Inbreeding coefficient (F_IS)
成株 Adults	NKS1	5	2.50	2.30	0.588	0.464	-0.267*
	NKS2	13	3.50	2.39	0.450	0.454	0.009
	NKS3	20	4.25	2.58	0.621	0.547	-0.134*
	NKS4	16	4.89	2.76	0.610	0.575	-0.061
	NKS5	7	3.88	2.68	0.542	0.568	0.047
	平均 Average	12.2	3.80	2.54	0.569	0.532	-0.070
种子 Seeds	NKS1	60	3.63	2.38	0.652	0.496	-0.315*
	NKS2	134	4.38	2.48	0.597	0.518	-0.150*
	NKS3	389	4.75	2.50	0.542	0.538	-0.007
	NKS4	168	4.25	2.59	0.654	0.571	-0.143*
	NKS5	29	2.38	1.97	0.455	0.370	-0.229*
	平均 Average	156	3.88	2.38	0.581	0.532	-0.092

观光木片断化居群的遗传多样性和交配系统

Genetic diversity and the mating system in a fragmented population of Tsoongiodendron odorum

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参数 Parameter	估计值 Estimates
多位点异交率 Multilocus outcrossing rate (t_m)	1.000(0.000)
单位点异交率 Singlelocus outcrossing rate (t_s)	0.997(0.002)
双亲近交比例 Biparental inbreeding (t_m-t_s)	0.003(0.002)
多位点父本相关性 Multilocus correlation of paternity (r_p(m))	0.229(0.043)
单位点父本相关性 Singlelocus correlation of paternity (r_p(s))	0.235(0.029)
r_p(s)- r_p(m)	0.007(0.020)
有效花粉供体的数目 No. of effective pollen donors (N_ep)	4.4

斑块 Stands	多位点异交率 Multilocus outcrossing rate (t_m)	双亲近交比例 Biparental inbreeding (t_m-t_s)	父本相关性 Correlation of paternity (r_p)	有效花粉供体数目 No. of effective pollen donors (N_ep)	r_p(s)- r_p(m)
NKS1	1.000(0.000)	0.000(0.000)	0.034(0.018)	29.4	-0.003(0.016)
NKS2	0.997(0.012)	0.006(0.012)	0.058(0.029)	17.2	0.030(0.018)
NKS3	0.994(0.016)	0.007(0.037)	0.233(0.016)	4.3	-0.009(0.018)
NKS4	1.000(0.000)	0.008(0.002)	0.124(0.037)	8.1	-0.010(0.022)
NKS5	1.000(0.000)	0.002(0.000)	0.063(0.035)	15.9	-0.033(0.033)

斑块 Stands	家系 Families	种子数 No. of seeds	多位点异交率 Multilocus outcrossing rate (t_m)	双亲近交 Biparental inbreeding (t_m-t_s)	父本相关性 Correlation of paternity (r_p)	有效花粉供体数目 No. of effective pollen donors (N_ep)
NKS1	N49	60	0.998(0.010)	0.058(0.012)	0.132(0.037)	7.6
NKS2	N17	30	0.986(0.000)	0.067(0.008)	0.131(0.035)	7.6
	N19	58	0.998(0.027)	0.044(0.010)	0.115(0.045)	8.7
	N24	46	0.979(0.033)	0.068(0.029)	0.111(0.037)	9.0
NKS3	N2	32	0.964(0.045)	0.057(0.039)	0.116(0.025)	8.6
	N4	59	0.998(0.004)	0.066(0.012)	0.081(0.027)	12.3
	N6	59	0.997(0.000)	0.050(0.008)	0.195(0.080)	5.1
	N9	60	0.998(0.010)	0.053(0.014)	0.100(0.041)	10.0
	N31	60	0.996(0.002)	0.057(0.010)	0.237(0.108)	4.2
	N33	60	0.925(0.073)	0.067(0.061)	0.141(0.049)	7.1
	N35	59	0.885(0.088)	0.000(0.071)	0.219(0.049)	4.6
NKS4	N39	63	0.999(0.000)	0.047(0.008)	0.220(0.078)	4.5
	N54	60	0.998(0.010)	0.034(0.010)	0.176(0.059)	5.7
	N55	45	0.995(0.000)	0.061(0.012)	0.132(0.047)	7.6
NKS5	N46	29	0.985(0.000)	0.077(0.016)	0.174(0.043)	5.7

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[15]	文亚峰, KentaroUchiyama, 韩文军, SaneyoshiUeno, 谢伟东, 徐刚标, YoshihikoTsumura. 微卫星标记中的无效等位基因[J]. 生物多样性, 2013, 21(1): 117-126.