关键词: 中华蜜蜂, 意大利蜜蜂, 蜂花粉, 扫描电子显微镜, DNA条形码

Abstract

Aims: The pollination behaviors of Apis cerana cerana (Acc) and Apis mellifera ligustica (Aml) have a significant meaning in maintaining ecosystem stability and improving crop yield and quality. However, their pollination behaviors are different. The aim of this study is to analyze the differences of pollinating habit and plant diversity between the two species under low temperature during the late autumn and winter periods.
Methods: We observed the pollination process of Acc and Aml, and collected the pollen in Xiasha Higher Education Campus of Hangzhou City during the late autumn and winter periods. The pollen morphology was analyzed by ordinary and scanning electron microscope, and further determined based on the gene ribulose 1,5-bisphosphate carboxylase (rbcl) amplified and DNA barcode. Finally, the diversity of pollinating plants was compared according to the pollen species during the late autumn and winter.
Results: Both Acc and Aml can pollinate the local plants during the late autumn and winter periods, but Acc had better adaptability and pollination frequency in the morning from 8:30 to 9:30 (P < 0.05). For different plants, Acc and Aml can pollinate many kinds of plant. Acc tended to collect pollen of Humulus scandens, Rosa chinensis, Eriobotrya laoshanica, and Hedera nepalensis var. sinensis, while Aml was partial to collect pollen of Ternstroemia gymnanthera, Chrysanthemum boreale, and Zinnia baageana.
Conclusions: In general, Acc and Aml can pollinate many kinds of plant during autumn and winter, and Acc have more extensive plant source. The investigation of pollination diversity and differences between Acc and Aml during the late autumn and winter periods will be helpful to study the pollination rules and preferences of honeybees, and provide theoretical for ecological protection in Hangzhou City.

Key words: Apis cerana cerana, Apis mellifera ligustica, bee pollen, scanning electron microscope, DNA barcode