生物多样性 ›› 2016, Vol. 24 ›› Issue (11): 1296-1305.DOI: 10.17520/biods.2016202

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基于DNA条形码的物种丰富度估计: 以宿迁地区鳞翅目蛾类为例

金倩1,2, 陈芬1, 罗桂杰1, 蔡卫佳1, 刘旭1, 王昊1, 杨采青2, 郝梦迪2, 张爱兵2,*()   

  1. 1.江苏省农业科学院宿迁农科所, 江苏宿迁 223800
    2.首都师范大学生命科学学院, 北京 100048
  • 收稿日期:2016-07-22 接受日期:2016-11-02 出版日期:2016-11-20 发布日期:2016-12-14
  • 通讯作者: 张爱兵
  • 基金资助:
    国家杰出青年基金(31425023)、国家自然科学基金(31272340)、国家自然科学基金委青年科学基金项目(31601877)和宿迁市自主创新项目(SQCX2015-06)

Estimation of species richness of moths (Insecta: Lepidoptera) based on DNA barcoding in Suqian, China

Qian Jin1,2, Fen Chen1, Guijie Luo1, Weijia Cai1, Xu Liu1, Hao Wang1, Caiqing Yang2, Mengdi Hao2, Aibing Zhang2,*()   

  1. 1 Suqian Institute of Agricultural Sciences, Jiangsu Academy of Agricultural Sciences, Suqian, Jiangsu 223800
    2 College of Life Sciences, Capital Normal University, Beijing 100048
  • Received:2016-07-22 Accepted:2016-11-02 Online:2016-11-20 Published:2016-12-14
  • Contact: Zhang Aibing

摘要:

为了探究基于DNA条形码方法量化物种多样性指标的可行性, 本研究以江苏省宿迁地区蛾类群落为例, 基于DNA条形码方法估计群落物种丰富度并绘制等级多度分布曲线(rank-abundance curves), 同时与基于传统形态学的对应指标进行比较。结果表明: (1)基于DNA条形码的物种丰富度估计与基于形态的物种丰富度估计之间没有显著差异; (2)基于形态和DNA条形码的等级多度分布曲线趋势一致, 通过K-S检测发现二者之间没有显著性差异(P > 0.05)。结果显示, 基于DNA条形码的物种丰富度估计能够在一定程度上补充基于形态学的方法, 可以尝试将其应用于蛾类群落生态学调查研究中。

关键词: 鳞翅目, DNA条形码, 物种丰富度, 等级多度分布曲线

Abstract:

To explore the feasibility of assessing species diversity using DNA barcoding, we investigated this approach by focusing on moths species (Lepidoptera) in Suqian, China. The study evaluated community species richness and rank-abundance curves using the DNA barcoding method, and compared it with the traditional morphology method. Results indicated that there was no significant difference between the DNA barcode-based approach and the morphology-based approach. All DNA barcode-based rank-abundance curves gave similar and clear patterns when compared with morphology-based curves (Kolmogorov-Smirnov two sample test, P > 0.05). Our results indicate that the DNA barcode-based approach is able to be used to estimate species richness.

Key words: Lepidoptera, DNA barcoding, species richness, rank-abundance curves