基于DNA条形码的物种丰富度估计: 以宿迁地区鳞翅目蛾类为例

doi:10.17520/biods.2016202

生物多样性 ›› 2016, Vol. 24 ›› Issue (11): 1296-1305. DOI: 10.17520/biods.2016202

所属专题：昆虫多样性与生态功能

• 研究报告: 动物多样性 • 上一篇下一篇

基于DNA条形码的物种丰富度估计: 以宿迁地区鳞翅目蛾类为例

金倩^1,², 陈芬¹, 罗桂杰¹, 蔡卫佳¹, 刘旭¹, 王昊¹, 杨采青², 郝梦迪², 张爱兵^2,^*()

1.江苏省农业科学院宿迁农科所, 江苏宿迁 223800
2.首都师范大学生命科学学院, 北京 100048

收稿日期:2016-07-22 接受日期:2016-11-02 出版日期:2016-11-20 发布日期:2016-12-14
通讯作者: 张爱兵
基金资助:
国家杰出青年基金(31425023)、国家自然科学基金(31272340)、国家自然科学基金委青年科学基金项目(31601877)和宿迁市自主创新项目(SQCX2015-06)

Estimation of species richness of moths (Insecta: Lepidoptera) based on DNA barcoding in Suqian, China

Qian Jin^1,², Fen Chen¹, Guijie Luo¹, Weijia Cai¹, Xu Liu¹, Hao Wang¹, Caiqing Yang², Mengdi Hao², Aibing Zhang^2,^*()

1 Suqian Institute of Agricultural Sciences, Jiangsu Academy of Agricultural Sciences, Suqian, Jiangsu 223800
2 College of Life Sciences, Capital Normal University, Beijing 100048

Received:2016-07-22 Accepted:2016-11-02 Online:2016-11-20 Published:2016-12-14
Contact: Zhang Aibing

摘要/Abstract

摘要：

为了探究基于DNA条形码方法量化物种多样性指标的可行性, 本研究以江苏省宿迁地区蛾类群落为例, 基于DNA条形码方法估计群落物种丰富度并绘制等级多度分布曲线(rank-abundance curves), 同时与基于传统形态学的对应指标进行比较。结果表明: (1)基于DNA条形码的物种丰富度估计与基于形态的物种丰富度估计之间没有显著差异; (2)基于形态和DNA条形码的等级多度分布曲线趋势一致, 通过K-S检测发现二者之间没有显著性差异(P > 0.05)。结果显示, 基于DNA条形码的物种丰富度估计能够在一定程度上补充基于形态学的方法, 可以尝试将其应用于蛾类群落生态学调查研究中。

关键词: 鳞翅目, DNA条形码, 物种丰富度, 等级多度分布曲线

Abstract

To explore the feasibility of assessing species diversity using DNA barcoding, we investigated this approach by focusing on moths species (Lepidoptera) in Suqian, China. The study evaluated community species richness and rank-abundance curves using the DNA barcoding method, and compared it with the traditional morphology method. Results indicated that there was no significant difference between the DNA barcode-based approach and the morphology-based approach. All DNA barcode-based rank-abundance curves gave similar and clear patterns when compared with morphology-based curves (Kolmogorov-Smirnov two sample test, P > 0.05). Our results indicate that the DNA barcode-based approach is able to be used to estimate species richness.

Key words: Lepidoptera, DNA barcoding, species richness, rank-abundance curves

金倩, 陈芬, 罗桂杰, 蔡卫佳, 刘旭, 王昊, 杨采青, 郝梦迪, 张爱兵 (2016) 基于DNA条形码的物种丰富度估计: 以宿迁地区鳞翅目蛾类为例. 生物多样性, 24, 1296-1305. DOI: 10.17520/biods.2016202.

Qian Jin, Fen Chen, Guijie Luo, Weijia Cai, Xu Liu, Hao Wang, Caiqing Yang, Mengdi Hao, Aibing Zhang (2016) Estimation of species richness of moths (Insecta: Lepidoptera) based on DNA barcoding in Suqian, China. Biodiversity Science, 24, 1296-1305. DOI: 10.17520/biods.2016202.

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链接本文: https://www.biodiversity-science.net/CN/10.17520/biods.2016202

https://www.biodiversity-science.net/CN/Y2016/V24/I11/1296

图/表 5

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MOTU^①	种名 Species^②	MOTU	种名 Species
MOTU01	Lacanobia contigua	MOTU34	Parapoynx vittalis
MOTU02	Agrotis segetum 黄地老虎	MOTU35	Heliothis assulta 烟青虫
MOTU03	Conogethes punctiferalis 桃蛀螟	MOTU36	Herminia grisealis
MOTU04	Nola cicatricalis	MOTU37	Spodoptera depravata
MOTU05	Hipoepa fractalis 中影单跗夜蛾	MOTU38	Scopula subpunctaria
MOTU06	Oraesia lata 平嘴壶夜蛾	MOTU39	Diaphania indica 瓜绢野螟
MOTU07	Spodoptera litura 斜纹夜蛾	MOTU40	Botyodes diniasalis 黄翅缀叶野螟
MOTU08	Uropyia meticulodina 核桃美舟蛾	MOTU41	Emmelia trabealis 谐夜蛾
MOTU09	Evergestis extimalis	MOTU42	Termioptycha nigrescens
MOTU10	Mythimna separate	MOTU43	Ipimorpha subtusa 杨逸色夜蛾
MOTU11	Ctenoplusia albostriata	MOTU44	Spilosoma lubricipeda
MOTU12	Peridea lativitta 侧带内斑舟蛾	MOTU45	Parapediasia teterrellus
MOTU13	Thyas juno 肖毛翅夜蛾	MOTU46	Adoxophyes orana
MOTU14	Nephopterix fumella	MOTU47	Simplicia rectalis 黑点贫夜蛾
MOTU15	Spodoptera exigua 甜菜夜蛾	MOTU48	Glyptoteles leucacrinella 亮雕斑螟
MOTU16	Oglasa consanguis	MOTU49	Somena scintillans
MOTU17	Diaphania perspectalis 黄杨绢野螟	MOTU50	Anadevidia peponis 葫芦夜蛾
MOTU18	Athetis lepigone	MOTU51	Hypocala subsatura 苹梢鹰夜蛾
MOTU19	Miyakea raddeella	MOTU52	Choristoneura diversana 异色卷蛾
MOTU20	Herpetogramma pseudomagna 狭翅切叶野螟	MOTU53	Choristoneura luticostana 棕色卷蛾
MOTU21	Axylia putris 朽木夜蛾	MOTU54	Archips betulana; Archips podana
MOTU22	Mocis ancilla	MOTU55	Palpita nigropunctalis 白蜡绢须野螟
MOTU23	Mecyna tricolor	MOTU56	Epiblema foenella 白钩小卷蛾
MOTU24	Mamestra brassicae 甘蓝夜蛾	MOTU57	Grammodes geometrica 象夜蛾
MOTU25	Xanthorhoe quadrifasiata	MOTU58; MOTU60	Plusia nadeja
MOTU26	Euproctis similis	MOTU59	Sphinx caligineus
MOTU27	Zanclognatha lunalis 朽镰须夜蛾	MOTU61	Acronicta rumicis 梨剑纹夜蛾
MOTU28	Theretra japonica	MOTU62	Herminia tarsipennalis
MOTU29	Herminia tarsicrinalis	MOTU63	Pangrapta trimantesalis
MOTU30	Eremodrina morosa	MOTU64	Eutelia hamulatrix 沟尾夜蛾
MOTU31	Helicoverpa armigera 棉铃虫	MOTU65	Plusilla rosalia
MOTU32	Nomophila noctuella	MOTU66	Bertula bistrigata
MOTU33	Dysgonia mandschuriana	MOTU67	Gastropacha populifolia 杨枯叶蛾

MOTU^①	种名 Species^②	MOTU	种名 Species
MOTU01	Lacanobia contigua	MOTU34	Parapoynx vittalis
MOTU02	Agrotis segetum 黄地老虎	MOTU35	Heliothis assulta 烟青虫
MOTU03	Conogethes punctiferalis 桃蛀螟	MOTU36	Herminia grisealis
MOTU04	Nola cicatricalis	MOTU37	Spodoptera depravata
MOTU05	Hipoepa fractalis 中影单跗夜蛾	MOTU38	Scopula subpunctaria
MOTU06	Oraesia lata 平嘴壶夜蛾	MOTU39	Diaphania indica 瓜绢野螟
MOTU07	Spodoptera litura 斜纹夜蛾	MOTU40	Botyodes diniasalis 黄翅缀叶野螟
MOTU08	Uropyia meticulodina 核桃美舟蛾	MOTU41	Emmelia trabealis 谐夜蛾
MOTU09	Evergestis extimalis	MOTU42	Termioptycha nigrescens
MOTU10	Mythimna separate	MOTU43	Ipimorpha subtusa 杨逸色夜蛾
MOTU11	Ctenoplusia albostriata	MOTU44	Spilosoma lubricipeda
MOTU12	Peridea lativitta 侧带内斑舟蛾	MOTU45	Parapediasia teterrellus
MOTU13	Thyas juno 肖毛翅夜蛾	MOTU46	Adoxophyes orana
MOTU14	Nephopterix fumella	MOTU47	Simplicia rectalis 黑点贫夜蛾
MOTU15	Spodoptera exigua 甜菜夜蛾	MOTU48	Glyptoteles leucacrinella 亮雕斑螟
MOTU16	Oglasa consanguis	MOTU49	Somena scintillans
MOTU17	Diaphania perspectalis 黄杨绢野螟	MOTU50	Anadevidia peponis 葫芦夜蛾
MOTU18	Athetis lepigone	MOTU51	Hypocala subsatura 苹梢鹰夜蛾
MOTU19	Miyakea raddeella	MOTU52	Choristoneura diversana 异色卷蛾
MOTU20	Herpetogramma pseudomagna 狭翅切叶野螟	MOTU53	Choristoneura luticostana 棕色卷蛾
MOTU21	Axylia putris 朽木夜蛾	MOTU54	Archips betulana; Archips podana
MOTU22	Mocis ancilla	MOTU55	Palpita nigropunctalis 白蜡绢须野螟
MOTU23	Mecyna tricolor	MOTU56	Epiblema foenella 白钩小卷蛾
MOTU24	Mamestra brassicae 甘蓝夜蛾	MOTU57	Grammodes geometrica 象夜蛾
MOTU25	Xanthorhoe quadrifasiata	MOTU58; MOTU60	Plusia nadeja
MOTU26	Euproctis similis	MOTU59	Sphinx caligineus
MOTU27	Zanclognatha lunalis 朽镰须夜蛾	MOTU61	Acronicta rumicis 梨剑纹夜蛾
MOTU28	Theretra japonica	MOTU62	Herminia tarsipennalis
MOTU29	Herminia tarsicrinalis	MOTU63	Pangrapta trimantesalis
MOTU30	Eremodrina morosa	MOTU64	Eutelia hamulatrix 沟尾夜蛾
MOTU31	Helicoverpa armigera 棉铃虫	MOTU65	Plusilla rosalia
MOTU32	Nomophila noctuella	MOTU66	Bertula bistrigata
MOTU33	Dysgonia mandschuriana	MOTU67	Gastropacha populifolia 杨枯叶蛾

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基于DNA条形码的物种丰富度估计: 以宿迁地区鳞翅目蛾类为例

Estimation of species richness of moths (Insecta: Lepidoptera) based on DNA barcoding in Suqian, China

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