不同干旱水平下蚯蚓对番茄抗旱能力的影响

doi:10.17520/biods.2021488

生物多样性 ›› 2022, Vol. 30 ›› Issue (7): 21488. DOI: 10.17520/biods.2021488

所属专题：土壤生物与土壤健康

• 研究报告: 动物多样性 • 上一篇下一篇

不同干旱水平下蚯蚓对番茄抗旱能力的影响

李季蔓¹^,², 靳楠¹^,², 胥毛刚¹^,², 霍举颂¹^,², 陈小云¹^,², 胡锋¹^,², 刘满强¹^,²^,^*()

1.南京农业大学资源与环境科学学院土壤生态实验室, 南京 210095
2.江苏省有机固体废弃物资源化协同创新中心, 南京 210095

收稿日期:2021-11-27 接受日期:2022-01-08 出版日期:2022-07-20 发布日期:2022-01-09
通讯作者: 刘满强
作者简介:*E-mail: liumq@njau.edu.cn
基金资助:
国家自然科学基金(42177286);国家自然科学基金(41771287);中央高校基本科研业务费专项资金

Effects of earthworm on tomato resistance under different drought levels

Jiman Li¹^,², Nan Jin¹^,², Maogang Xu¹^,², Jusong Huo¹^,², Xiaoyun Chen¹^,², Feng Hu¹^,², Manqiang Liu¹^,²^,^*()

1. Soil Ecology Lab, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095
2. Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Nanjing 210095

Received:2021-11-27 Accepted:2022-01-08 Online:2022-07-20 Published:2022-01-09
Contact: Manqiang Liu

摘要/Abstract

摘要：

土壤无脊椎动物可能会通过促进土壤持水能力和增加土壤肥力而缓解植物的干旱胁迫。本研究采用蚯蚓和干旱水平的双因子完全交互设计, 模拟了干旱胁迫条件下蚯蚓对土壤性质及番茄抗旱性的影响。结果表明, 在高干旱胁迫时, 蚯蚓通过增加番茄茎叶抗氧化能力提高了植物抗旱性, 上调番茄茎叶脱落酸和茉莉酸生物合成过程的基因表达(NCED、NSY、OPR、AOS和LOX), 促进脱落酸和茉莉酸含量分别增加43.2%和33.6%, 过氧化氢酶、过氧化物酶和超氧化物歧化酶含量分别增加12.9%、8.4%和47.3%。在低干旱胁迫时, 蚯蚓上调茉莉酸合成通路基因表达, 但降低了脱落酸含量, 对转录因子ABF4、MYC2基因表达和植物抗氧化能力无明显影响。干旱导致的土壤水分和养分条件变化影响着蚯蚓介导的植物抗旱性响应。本研究证明了土壤动物对植物抗旱的重要作用, 如蚯蚓对植物激素合成、信号传导和抗氧化能力的影响。了解土壤动物影响植物抗旱的内在机制, 有助于深挖和利用土壤动物的多样化生态功能。

关键词: 蚯蚓, 气候变化, 地上和地下部, 植物激素, 信号通路

Abstract

Aims: Soil invertebrates have the potential to modify plant responses to drought stress. For example, some functional groups improve soil conditions via resource provision and water retention, potentially alleviating drought stress. This study was designed in order to mechanistically understand the functional roles of soil fauna in ecosystem services.
Methods: A pot experiment using tomatoes (Lycopersicon esculentum) was conducted in a greenhouse. This study adopted a complete factorial design that manipulated earthworm (Metaphire guillelmi) abundance and drought stress level.
Results: Our results indicate that earthworms promote plant drought resistance under high drought stress, which significantly up-regulated gene expression (NCED, NSY, OPR, AOS and LOX) of abscisic acid and jasmonic acid biosynthesis, promoted the accumulation of abscisic acid and jasmonic acid by 43.2% and 33.6%, up-regulated transcription factor expression, and increased the activities of catalase, peroxidase and superoxide dismutase by 12.9%, 8.4% and 47.3% in tomato shoots, respectively. However, under low drought stress, earthworms up-regulated jasmonic acid synthesis pathway gene expression, but reduced abscisic acid content, and had no significant effect on the expression of transcription factors ABF4 and MYC2 genes or plant antioxidant capacity.
Conclusion: The effects of earthworms on plant resistance depends on drought level. This study confirms the importance of soil invertebrates in plant drought resistance including the potential impact of earthworms on plant hormone synthesis, signal transduction, and antioxidant capacity.

Key words: earthworms, global climate change, aboveground-belowground, plant hormone, signal transduction

李季蔓, 靳楠, 胥毛刚, 霍举颂, 陈小云, 胡锋, 刘满强 (2022) 不同干旱水平下蚯蚓对番茄抗旱能力的影响. 生物多样性, 30, 21488. DOI: 10.17520/biods.2021488.

Jiman Li, Nan Jin, Maogang Xu, Jusong Huo, Xiaoyun Chen, Feng Hu, Manqiang Liu (2022) Effects of earthworm on tomato resistance under different drought levels. Biodiversity Science, 30, 21488. DOI: 10.17520/biods.2021488.

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链接本文: https://www.biodiversity-science.net/CN/10.17520/biods.2021488

https://www.biodiversity-science.net/CN/Y2022/V30/I7/21488

图/表 5

图1 不同干旱胁迫条件下, 威廉腔环蚓的生物量变化以及蚯蚓对土壤理化性质(土壤含水量、矿质态氮、速效磷、微生物生物量碳和微生物生物量氮含量)的影响(平均值 ± 标准差, n = 5)。采用双因素方差分析评估蚯蚓和干旱胁迫对土壤含水量的影响。* P < 0.05, ** P < 0.01, *** P < 0.001。

Fig. 1 Changes in biomass of Metaphire guillelmi and effects of earthworm on soil physical and chemical properties (soil water content, mineral nitrogen, available phosphorus, microbial biomass carbon and microbial biomass nitrogen) under low and high drought conditions (mean ± SD, n = 5). Statistical differences were assessed following a two-way ANOVA using earthworm and drought as factors. * P < 0.05, ** P < 0.01, *** P < 0.001.

图2 不同干旱胁迫条件下, 蚯蚓对番茄茎叶中的过氧化氢酶、过氧化物酶、超氧化物歧化酶和丙二醛含量的影响(平均值 ± 标准差, n = 5)。采用双因素方差分析评估蚯蚓和干旱胁迫对植物抗氧化指标的影响。* P < 0.05, ** P < 0.01, *** P < 0.001。

Fig. 2 Effects of earthworm on the contents of tomato shoot catalase (CAT), peroxidase (POD), superoxide dismutase (SOD) and malondialdehyde (MDA) under low and high drought conditions (mean ± SD, n = 5). Statistical differences were assessed following a two-way ANOVA using earthworm and drought as factors. * P < 0.05, ** P < 0.01, *** P < 0.001.

图3 蚯蚓对番茄抗旱激素信号通路的影响。(A)不同干旱胁迫条件下蚯蚓上调的番茄茎叶ABA和JA信号通路(M00372, M00113)差异基因数量(n = 5); (B)蚯蚓对相关基因表达量的影响, 数据为log2转换的表达式平均值, 符合校正P值 < 0.05; (C)蚯蚓对番茄茎叶ABA和JA含量的影响(* P < 0.05, ** P < 0.01, *** P < 0.001)。

Fig. 3 Effects of earthworm on drought resistance hormone signaling pathway in tomato shoot. (A) Venn diagram of up-regulated DEGs of tomato shoot ABA and JA signaling pathways (M00372, M00113) in response to earthworm. (B) Expression of ABA and JA signaling pathway-related genes in response to earthworm, data are mean values of log2-transformed expression values, false discovery rate-corrected P < 0.05 (n = 5). (C) Effects of earthworm on the contents of tomato shoot abscisic acid and jasmonic acid under low and high drought stress conditions (* P < 0.05, ** P < 0.01, *** P < 0.001).

图4 蚯蚓介导的土壤生物和非生物性质(矿质态氮、速效磷、微生物生物量碳和微生物生物量氮)与植物抗旱化合物(过氧化氢酶CAT、过氧化物酶POD、超氧化物歧化酶SOD、丙二醛MDA、脱落酸ABA和茉莉酸JA)的相关性分析。* P < 0.05, ** P < 0.01, *** P < 0.001。

Fig.4 Correlation analysis between soil biotic and abiotic properties and tomato drought-resistance compounds. CAT, Catalase; POD, Peroxidase; SOD, Superoxide dismutase; MDA, Malondialdehyde; ABA, Abscisic acid; JA, Jasmonic acid. * P < 0.05, ** P < 0.01, *** P < 0.001.

图5 不同干旱胁迫条件下, 蚯蚓影响番茄茎叶ABA和JA信号转导相关生理机制的概念模型。图中η代表上调作用, ι代表下调作用。NCED: 9-顺-环氧类胡萝卜素双加氧酶; AOS: 丙二烯氧化物合酶; OPR: OPDA还原酶; ABF: ABA响应元素结合因子; NSY: 新黄质合成酶; LOX: 脂氧合酶; MYC2: 髓细胞组织增生蛋白; JAZs: 茉莉酸转录抑制因子; CAT: 过氧化氢酶; POD: 过氧化物酶; SOD: 超氧化物歧化酶。

Fig. 5 Proposed model of earthworm induced resistance to drought stress in tomato. η and ι represent up- and down-regulation respectively. NCED, 9-cis-epoxycarotenoid dioxygenase; AOS, allene oxide synthase; OPR, 12-oxo-phytodienoic acid reductase; ABF, ABA-responsive element binding factor; NSY, Neoxanthinsynthase; LOX, Lipoxygenase; MYC2, Myelocytomatosis proteins 2; JAZs, Jasmonate ZIM-domain; CAT, Catalase; POD, Peroxidase; SOD, Superoxide dismutase.

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不同干旱水平下蚯蚓对番茄抗旱能力的影响

Effects of earthworm on tomato resistance under different drought levels

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