采集策略对叶附生苔类植物发现概率及物种多样性的重要性

doi:10.17520/biods.2022685

生物多样性 ›› 2023, Vol. 31 ›› Issue (4): 22685. DOI: 10.17520/biods.2022685 cstr: 32101.14.biods.2022685

采集策略对叶附生苔类植物发现概率及物种多样性的重要性

姚雪¹, 陈星¹, 戴尊¹, 宋坤²^,^*(), 邢诗晨¹, 曹宏彧¹, 邹璐¹, 王健¹^,³^,^*()

1.华东师范大学生命科学学院, 上海 200241
2.华东师范大学生态与环境科学学院, 上海 200241
3.上海崇明生态研究院, 上海 202162

收稿日期:2022-12-17 接受日期:2023-03-24 出版日期:2023-04-20 发布日期:2023-04-20
通讯作者: *E-mail: jwang@bio.ecnu.edu.cn; ksong@des.ecnu.edu.cn
基金资助:
国家自然科学基金(32070228);国家自然科学基金(31770371);百山祖国家公园科研项目(2021KFLY09)

Importance of collection strategy on detection probability and species diversity of epiphyllous liverworts

Xue Yao¹, Xing Chen¹, Zun Dai¹, Kun Song²^,^*(), Shichen Xing¹, Hongyu Cao¹, Lu Zou¹, Jian Wang¹^,³^,^*()

1. School of Life Sciences, East China Normal University, Shanghai 200241
2. School of Ecological and Environmental Sciences, East China Normal University, Shanghai 200241
3. Shanghai Insitute of Eco-Chongming (SIEC), Shanghai 202162

Received:2022-12-17 Accepted:2023-03-24 Online:2023-04-20 Published:2023-04-20
Contact: *E-mail: jwang@bio.ecnu.edu.cn; ksong@des.ecnu.edu.cn

摘要/Abstract

摘要：

叶附生苔类植物主要生长在热带和亚热带常绿阔叶林维管植物叶片的表面, 是苔藓植物中较为复杂的一个类群, 特殊的生理生态特性使其在成为气候变化指示类群方面的潜力很大。为了探究采集者的经验及采样强度是否显著影响叶附生苔的发现概率及物种多样性, 本研究在乌岩岭国家级自然保护区对叶附生苔开展了系统的采集和比较研究, 以期为我国其他地区开展叶附生苔类植物的调查和研究提供经验和方法支持。通过4位具有不同采集经验的采集者在规定的时间(每人每次30 min)和区域(铁炉基至黄家岱)内对叶附生苔进行3次重复采集, 分析不同采集者所采集到的含有叶附生苔的叶片数及所获得的物种多样性之间的差异。结果显示, 4位采集者共采集叶附生苔类植物5科11属36种, 并新增1种首次发现附生于叶片的种类(南溪苔Makinoa crispata)。采集者经验对叶附生苔的发现概率影响不显著, 但对叶附生苔的物种多样性, 尤其是一般种和优势种的多样性具有显著影响, 推测这一差异主要是由采集者不同的采集策略所导致。同时, 本研究结果还表明, 叶附生苔的物种多样性随着采样强度的增加而增加, 4位采集者共同参与的采样结果体现出较高的采样充分性。本研究结果表明即使针对叶附生苔类植物这一类常被认为采集和鉴定非常困难的类群, 如加以必要的采集前的经验培训, 即使是非专业的采集人员也足以开展针对叶附生苔类植物的调查。

关键词: 气候变化, 苔藓植物, 采样方法, 乌岩岭国家级自然保护区, 物种多样性

Abstract

Aims: Epiphyllous liverworts mainly grow on the leaf surface of vascular plants in tropical and subtropical evergreen broad-leaved forests, which are a complex group of bryophytes. Due to their special physiological and ecological characteristics, epiphyllous liverworts have great potential to become an indicator of climate change. To achieve this goal, detailed information on species diversity and distribution is required. In order to explore whether the experience and sampling intensity of observers significantly affects the detection probability and species diversity of epiphyllous liverworts in field surveys, the present study conducted systematic sampling and comparative analysis of the epiphyllous liverworts in the Wuyanling National Nature Reserve.

Methods: Through three repeated collections by four collectors with different collection experience at the same time (30 minutes per person) and in the same area (Tieluji to Huangjiadai), this study analyzed the differences between the number of leaves with epiphyllous liverworts and liverwort diversity among the four observers.

Results: There are 36 species of epiphyllous liverworts obtained from 11 genera and 5 families. One species (Makinoa crispata) was newly recorded in the area and reported as epiphyllous species for the first time. The observer’s experience has no obvious impact on the detection probability of epiphyllous liverworts, but has a significant impact on the species diversity, especially for the diversity of general species and dominant species. It is speculated that this difference is mainly caused by the collection strategy of different observers. Meanwhile, this study also showed that the species diversity of epiphyllous liverworts increased with the increase of sampling intensity, and the collection results jointly participated by the four observers reflected a high sampling adequacy.

Conclusion: The results show that for epiphyllous liverworts, a group that is often considered difficult to collect and identify, with necessary pre-collection experience training, non-professional observers also can perform conduct surveys of epiphyllous liverworts.

Key words: climate change, bryophytes, sampling method, Wuyanling National Nature Reserve, species diversity

姚雪, 陈星, 戴尊, 宋坤, 邢诗晨, 曹宏彧, 邹璐, 王健 (2023) 采集策略对叶附生苔类植物发现概率及物种多样性的重要性. 生物多样性, 31, 22685. DOI: 10.17520/biods.2022685.

Xue Yao, Xing Chen, Zun Dai, Kun Song, Shichen Xing, Hongyu Cao, Lu Zou, Jian Wang (2023) Importance of collection strategy on detection probability and species diversity of epiphyllous liverworts. Biodiversity Science, 31, 22685. DOI: 10.17520/biods.2022685.

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链接本文: https://www.biodiversity-science.net/CN/10.17520/biods.2022685

https://www.biodiversity-science.net/CN/Y2023/V31/I4/22685

图/表 6

图1 浙江省乌岩岭国家级自然保护区叶附生苔调查样线

Fig. 1 Map of sampling line of epiphyllous liverworts in the Wuyanling National Nature Reserve, Zhejiang Province

图2 4位采集者所采集的长有叶附生苔类植物的叶片数(a)和采集到的物种数(b)箱型图(箱中实线表示中值, A、B、C、D表示采集经验丰富程度由弱到强)

Fig. 2 Box plot of the number of leaves (a) and species (b) of epiphyllous liverworts collected by four observers (solid line in the box shows the median value, A, B, C, and D indicate the degree of collection experience from weak to strong)

图3 4位采集者采集到的叶附生苔类植物种类的Venn图(A、B、C、D表示采集经验丰富程度由弱到强)

Fig. 3 Venn diagram of collected epiphyllous liverworts among four observers (A, B, C, and D indicate the degree of collection experience from weak to strong)

图4 4位采集者3次分别采集到的物种数(A、B、C、D表示采集经验丰富程度由弱到强)

Fig. 4 Species number of epiphyllous liverworts collected by four observers in three sampling efforts (A, B, C, and D indicate the degree of collection experience from weak to strong)

图5 基于叶片数(a)和采样覆盖度(b)的不同位阶下各采集者叶附生苔多样性内插和外推采样曲线、采样充分性曲线(c) (A、B、C、D表示采集经验丰富程度由弱到强)

Fig. 5 The species richness interpolation and extrapolation sampling curve and sampling adequacy curve (c) of epiphyllous liverworts collected by each observer based on the number of leaves (a) and sampling coverage (b) at different rank (A, B, C, and D indicate the degree of collection experience from weak to strong)

图6 基于叶片数的叶附生苔类植物物种累积曲线及预测值曲线

Fig. 6 Cumulative curve and predicted value curve of epiphyllous liverworts based on leaf numbers

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采集策略对叶附生苔类植物发现概率及物种多样性的重要性

Importance of collection strategy on detection probability and species diversity of epiphyllous liverworts

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