东乡野生稻可培养内生细菌群落组成及多样性

doi:10.17520/biods.2019219

生物多样性 ›› 2019, Vol. 27 ›› Issue (12): 1320-1329. DOI: 10.17520/biods.2019219

• 研究报告: 微生物多样性 • 上一篇下一篇

东乡野生稻可培养内生细菌群落组成及多样性

陈志远¹,刘珺¹,杨星鹏¹,刘梦¹,汪涯²,张志斌^1,^*(),朱笃^1,^2,^*

1 江西师范大学生命科学学院, 江西省亚热带植物资源保护与利用重点实验室, 南昌 330022
2 江西科技师范大学生命科学学院, 江西省生物加工过程重点实验室, 南昌 330013

收稿日期:2019-07-07 接受日期:2019-10-20 出版日期:2019-12-20 发布日期:2020-02-22
通讯作者: 张志斌,朱笃
基金资助:
国家自然科学基金(31760160);江西省自然科学基金(20171BAB204009);江西省自然科学基金(20181BAB215044);江西师范大学研究生创新项目(YJS2018080);江西师范大学研究生创新项目(YC2019-S121)

Community composition and diversity of cultivable endophytic bacteria isolated from Dongxiang wild rice

Zhiyuan Chen¹,Jun Liu¹,Xingpeng Yang¹,Meng Liu¹,Ya Wang²,Zhibin Zhang^1,^*(),Du Zhu^1,^2,^*

1 Key Laboratory of Protection and Utilization of Subtropical Plant Resources of Jiangxi Province, College of Life Sciences, Jiangxi Normal University, Nanchang 330022
2 Key Laboratory of Bioprocess Engineering of Jiangxi Province, College of Life Sciences, Jiangxi Science and Technology Normal University, Nanchang 330013

Received:2019-07-07 Accepted:2019-10-20 Online:2019-12-20 Published:2020-02-22
Contact: Zhang Zhibin,Zhu Du

摘要/Abstract

摘要：

内生细菌对宿主植物生长发育和生理代谢具有重要影响, 揭示其群落组成和多样性具有重要的生物学和生态学意义。本研究采用可培养法从东乡野生稻(Oryza rufipogon)不同组织中分离获得94株内生细菌, 基于16S rRNA基因序列比对和系统发育分析将其归属于变形菌门、放线菌门和厚壁菌门3门14科17属, 其中芽孢杆菌属(Bacillus)和微小菌属(Microbacterium)为优势属, 分别占总株数的27.7%和20.2%。不同组织中内生细菌的分布和多样性存在差异, 根中内生细菌数量最多(34株, 36.2%), 叶次之(32株, 34.0%), 茎最少(28株, 29.8%); 根中内生细菌Shannon-Wiener多样性指数(H′ = 2.52)、Simpson优势度指数(D = 0.88)和Pielou均匀度指数(E = 0.72)均高于茎与叶, 根和茎的Jaccard相似性系数(C = 0.29)低于根和叶(C = 0.47)以及茎和叶(C = 0.45)。研究结果表明, 东乡野生稻内生细菌具有丰富的物种多样性, 并且不同组织部位的内生细菌组成存在差异, 根中内生细菌多样性最丰富。

关键词: 野生稻, 植物内生菌, 分类, 系统发育, 组成分析

Abstract

Endophytic bacteria are essential to the growth and metabolism of their host plants. To better understand the community structure and diversity of endophytic bacteria on plants, we isolated 94 strains of endophytic bacteria from the roots, stems and leaves of Dongxiang wild rice (Oryza rufipogon). Subsequent 16S rRNA sequence alignments and phylogenetic analyses revealed that the 94 strains could be categorized into 17 genera of 14 families in 3 phyla (Proteobacteria, Actinobacteria, and Firmicutes).Bacillus and Microbacterium, accounting for 27.7% and 20.2% of the diversity, respectively, were the two most dominant genera. The distribution and diversity of endophytic bacteria differed across different tissues of Dongxiang wild rice: the roots contained the most endophytic bacteria strains (n = 34, 36.2%), followed by leaves (n = 32, 34.0%), and stems (n = 28, 29.8%). Shannon-Wiener diversity index (H′ = 2.52), Simpson dominance index (D = 0.88) and Pielou evenness index (E = 0.72) were higher in the roots than in the stems or leaves. Meanwhile, the Jaccard similarity index between roots and stems (C = 0.29) was lower than that between roots and leaves (C = 0.47), or that between stems and leaves (C = 0.45). Our efforts to uncover the diversity of endophytic bacteria in Dongxiang wild rice confirms that roots contain the most diverse strains but that the composition of endophytes varies across tissues.

Key words: Oryza rufipogon, endophytes, classification, phylogeny, composition analysis

陈志远, 刘珺, 杨星鹏, 刘梦, 汪涯, 张志斌, 朱笃 (2019) 东乡野生稻可培养内生细菌群落组成及多样性. 生物多样性, 27, 1320-1329. DOI: 10.17520/biods.2019219.

Zhiyuan Chen, Jun Liu, Xingpeng Yang, Meng Liu, Ya Wang, Zhibin Zhang, Du Zhu (2019) Community composition and diversity of cultivable endophytic bacteria isolated from Dongxiang wild rice. Biodiversity Science, 27, 1320-1329. DOI: 10.17520/biods.2019219.

导出引用管理器 EndNote|Ris|BibTeX

链接本文: https://www.biodiversity-science.net/CN/10.17520/biods.2019219

https://www.biodiversity-science.net/CN/Y2019/V27/I12/1320

图/表 5

参考文献 48

[1]	Baldan E, Nigris S, Populin F, Zottini M, Squartini A, Baldan B ( 2014) Identification of culturable bacterial endophyte community isolated from tissues of Vitis vinifera “Glera”. Plant Biosystems, 148, 508-516.
[2]	Brundrett MC, Tedersoo L ( 2018) Evolutionary history of mycorrhizal symbioses and global host plant diversity. New Phytologist, 220, 1108-1115.
[3]	Chen JK, Wang HY, He GQ ( 1998) A survey on the habitats of Oryza rufipogon and Isoetes sinensis in Jiangxi Province. Chinese Biodiversity, 6, 260-266. (in Chinese with English abstract)
	[ 陈家宽, 王海洋, 何国庆 ( 1998) 江西境内珍稀植物普通野生稻和中华水韭产地的考察. 生物多样性, 6, 260-266.]
[4]	Dai F, Huang YH, Peng WM, Li LM, Long ZE ( 2011) Studies on the microbial community in the rhizosphere soil of Dongxiang wild rice. Agricultural Science & Technology, 12, 527-529.
[5]	Dominika T, Michal Z, Renata GK, Sonia S, Christel B, Katarzyna H ( 2018) Cadmium-induced changes in the production of siderophores by a plant growth promoting strain of Pseudomonas fulva. Journal of Basic Microbiology, 58, 623-632.
[6]	Dong XZ, Cai MY ( 2001) Manual for Systematic Identification of Common Bacteria. Science Press, Beijing. (in Chinese)
	[ 东秀珠, 蔡妙英 ( 2001) 常见细菌系统鉴定手册. 科学出版社, 北京.]
[7]	Faria DC, Dias ACF, Melo IS, de Carvalho Costa FE ( 2013) Endophytic bacteria isolated from orchid and their potential to promote plant growth. World Journal of Microbiology & Biotechnology, 29, 217-221.
[8]	Gerry AJ, Rashid AY, Mahmoud WY ( 2017) Genome sequencing of Microbacterium sp. Yaish 1, a bacterial strain isolated from the rhizosphere of date palm trees affected by salinity. Microbiology Resource Announcements, 5, e01247-17.
[9]	Greenberg JH ( 1956) The measurement of linguistic diversity. Language, 32, 105-119.
[10]	Hu GP, You MS, Liu B, Zhu YJ, Zheng XF, Lin YZ ( 2010) Relationship between the stem endophytic and rhizosphere bacteria and the variety characteristics of Oryza sativa. Chinese Journal of Tropical Crops, 31, 1026-1030. (in Chinese with English abstract)
	[ 胡桂萍, 尤民生, 刘波, 朱育菁, 郑雪芳, 林营志 ( 2010) 水稻茎部内生细菌及根际细菌与水稻品种特性的相关性. 热带作物学报, 31, 1026-1030.]
[11]	James EK, Olivares FL ( 1998) Infection and colonization of sugar cane and other graminaceous plants by endophytic diazotrophs. Critical Reviews in Plant Sciences, 17, 77-119.
[12]	Li F, Guo SY, Zhao Y, Chen DZ, Chong K, Xu YY ( 2010) Overexpression of a homopeptide repeat-containing bHLH protein gene (OrbHLH001) from Dongxiang wild rice confers freezing and salt tolerance in transgenic Arabidopsis. Plant Cell Reports, 29, 977-986.
[13]	Li GC, Zhang SY, Xiao W, Long ZY, Zhang NM ( 2015) Research progress on endophytes in rice. Chinese Agricultural Science Bulletin, 31(12), 157-162. (in Chinese with English abstract)
	[ 李龚程, 张仕颖, 肖炜, 龙智勇, 张乃明 ( 2015) 水稻中内生菌研究进展. 中国农学通报, 31(12), 157-162.]
[14]	Li NN, Li N, Cao YH, Zhang X, Xiao M, Liu Y, Wang WP ( 2017) Diversity of endophytic bacterial communities in three parental seeds of hybrid rice (Oryza sativa L.) at maturity stage. Journal of Food Science and Technology, 35(4), 56-64. (in Chinese with English abstract)
	[ 李南南, 黎妮, 曹艳花, 张欣, 肖明, 刘洋, 王伟平 ( 2017) 3个杂交水稻亲本成熟期种子内生细菌多样性研究. 食品科学技术学报, 35(4), 56-64.]
[15]	Li QQ, Jiao C, Nong Q, Yuan GQ, Lin W, Huang YL ( 2010) Dynamic distribution of endophytic bacteria in rice from Guangxi and their antagonism to the pathogen of rice sheath blight. Chinese Journal of Biological Control, 26, 312-319. (in Chinese with English abstract)
	[ 黎起秦, 焦成, 农倩, 袁高庆, 林纬, 黄永禄 ( 2010) 广西水稻内生细菌的动态分布及其对水稻纹枯病菌的拮抗作用. 中国生物防治, 26, 312-319.]
[16]	Liu XM, Yao Z, Shim JM, Lee KW, Kim HJ, Kim JH ( 2017) Properties of antimicrobial substances produced by Bacillus species isolated from rice straw. Microbiology and Biotechnology Letters, 45, 133-142.
[17]	Loaces I, Ferrando L, Scavino AF ( 2011) Dynamics, diversity and function of endophytic siderophore-producing bacteria in rice. Microbial Ecology, 61, 606-618.
[18]	Luo F, Wang Y, Zeng QG, Yan RM, Zhang ZB, Zhu D ( 2011) Diversity and plant growth promoting activities of the cultivable rhizo-bacteria of Dongxiang wild rice (Oryza rufipogon Griff.). Biodiversity Science, 19, 476-484. (in Chinese with English abstract)
	[ 罗菲, 汪涯, 曾庆桂, 颜日明, 张志斌, 朱笃 ( 2011) 东乡野生稻根际可培养细菌多样性及其植物促生活性分析. 生物多样性, 19, 476-484.]
[19]	Mano H, Morisaki H ( 2008) Endophytic bacteria in the rice plant. Microbes and Environments, 23, 109-117.
[20]	Marcus S, Ajay S, Owen PW ( 2004) Developments in the use of Bacillus species for industrial production. Canadian Journal of Microbiology, 50, 1-17.
[21]	Nelson EB ( 2004) Microbial dynamics and interactions in the spermosphere. Annual Review of Phytopathology, 42, 271-309.
[22]	Pavlova AS, Leontieva MR, Smirnova TA, Kolomeitseva GL, Netrusov AI, Tsavkelova EA ( 2017) Colonization strategy of the endophytic plant growth-promoting strains of Pseudomonas fluorescens and Klebsiella oxytoca on the seeds, seedlings and roots of the epiphytic orchid, Dendrobium nobile Lindl. Journal of Applied Microbiology, 123, 217-232.
[23]	Pielou EC ( 1966) The measurement of diversity in different types of biological collections. Journal of Theoretical Biology, 13, 131-144.
[24]	Quan RD, Wang J, Hui J, Bai HB, Lyu XL, Zhu YX, Zhang HW, Zhang ZJ, Li SH, Huang RF ( 2018) Improvement of salt tolerance using wild rice genes. Frontiers in Plant Science, 8, 2269-2280.
[25]	Rangjaroen C, Rerkasem B, Teaumroong N, Sungthong R, Lumyong S ( 2014) Comparative study of endophytic and endophytic diazotrophic bacterial communities across rice landraces grown in the highlands of northern Thailand. Archives of Microbiology, 196, 35-49.
[26]	Reinhold-Hurek B, Hurek T ( 2011) Living inside plants: Bacterial endophytes. Current Opinion in Plant Biology, 14, 435-443.
[27]	Ryan RP, Germaine K, Franks A, Ryan DJ, Dowling DN ( 2008) Bacterial endophytes: Recent developments and applications. FEMS Microbiology Letters, 278, 1-9.
[28]	Santoyo G, Moreno-Hagelsieb G, Orozco-Mosqueda MDC, Glick BR ( 2016) Plant growth-promoting bacterial endophytes. Microbiological Research, 183, 92-99.
[29]	Sha YX, Zeng QC, Wang X, Shen RQ, Liu H, Wang XG ( 2018) Screening and control efficiency evaluation of Bacillus against rice blast Magnaporthe oryzae. Chinese Journal of Biological Control, 34, 414-422. (in Chinese with English abstract)
	[ 沙月霞, 曾庆超, 王昕, 沈瑞清, 刘浩, 王喜刚 ( 2018) 防治稻瘟病芽胞杆菌的筛选及效果评价. 中国生物防治学报, 34, 414-422.]
[30]	Shahzad R, Waqas M, Khan AL, Al-Hosni K, Kang SM, Seo WC, Lee IJ ( 2017) Indoleacetic acid production and plant growth promoting potential of bacterial endophytes isolated from rice (Oryza sativa L.) seeds. Acta Biologica Hungarica, 68, 175-186.
[31]	Shannon CE ( 1948) A mathematical theory of communication. Bell System Technical Journal, 27, 379-423.
[32]	Shao SC, Burgess KS, Jennifer CJ, Liu Q, Fan XL, Hui H, Gao JY ( 2017) Using in situ symbiotic seed germination to restore over-collected medicinal orchids in Southwest China. Frontiers in Plant Science, 8, 888-898.
[33]	Shylla A, Shivaprakash MK, Shashidhar HE, Vishwakarma P, Sudradhar M ( 2016) Production of phytohormones by endophytic bacteria isolated from aerobic rice. Journal of Pure and Applied Microbiology, 10, 2127-2133.
[34]	Silo-Suh LA, Lethbridge BJ, Raffel SJ, Yin HH, Clardy J, Handelsman J ( 1994) Biological activities of two fungistatic antibiotics produced by Bacillus cereus UW85. Applied and Environmental Microbiology, 60, 2023-2030.
[35]	Song ZP, Chen JK, Zhao Y ( 2018) Rice domestication and the Yangtze River civilization. Biodiversity Science, 26, 346-356. (in Chinese with English abstract)
	[ 宋志平, 陈家宽, 赵耀 ( 2018) 水稻驯化与长江文明. 生物多样性, 26, 346-356.]
[36]	Sun L ( 2006) Endophytic Bacteria and Root-Associated Bacteria with Rice by Culture-Independent and Culture-Dependent Approaches. PhD dissertation, Capital Normal University, Beijing. (in Chinese with English abstract)
	[ 孙磊 ( 2006) 非培养方法和培养方法对水稻内生细菌和根结合细菌的研究. 博士学位论文, 首都师范大学, 北京.]
[37]	Tan ZY, Peng GX, Xu PZ, Ai SY, Tang SH, Zhang GX, Zeng FY ( 2009) Diversity and high nitrogenase activity of endophytic diazotrophs isolated from Oryza rufipogon Griff. Chinese Science Bulletin, 54, 2839-2848. (in Chinese with English abstract)
	[ 谭志远, 彭桂香, 徐培智, 艾绍英, 唐拴虎, 张国霞, 曾凤云 ( 2009) 普通野生稻(Oryza rufipogon)内生固氮菌多样性及高固氮酶活性. 科学通报, 54, 2839-2848.]
[38]	Tsavkelova EA, Cherdyntseva TA, Botina SG, Netrusov AI ( 2007) Bacteria associated with orchid roots and microbial production of auxin. Microbiological Research, 162, 69-76.
[39]	Tsavkelova EA, Egorova MA, Leontieva MR, Malakho SG, Kolomeitseva GL ( 2016) Dendrobium nobile Lindl. seed germination in co-cultures with diverse associated bacteria. Plant Growth Regulation, 80, 79-91.
[40]	Wang XJ, Jia RZ, Guo YL, Xu L, Zuo J, Kong H, Guo AP ( 2015) Diversity of culturable endobacterial communities in rice (Oryza sativa L.) stem at different growth stages. Chinese Journal of Tropical Crops, 36, 1078-1085. (in Chinese with English abstract)
	[ 王雪君, 贾瑞宗, 郭运玲, 徐林, 左娇, 孔华, 郭安平 ( 2015) 水稻4个生长时期茎部可培养内生菌多样性分析. 热带作物学报, 36, 1078-1085.]
[41]	Whittaker RH ( 1972) Evolution and measurement of species diversity. Taxon, 21, 213-251.
[42]	Xia H, Lu BR, Su J, Chen R, Rong J, Song ZP, Wang F ( 2009) Normal expression of insect-resistant transgene in progeny of common wild rice crossed with genetically modified rice: Its implication in ecological biosafety assessment. Theoretical and Applied Genetics, 119, 635-644.
[43]	Xie ZC, Chu YK, Zhang WJ, Lang DY, Zhang XH ( 2019) Bacillus pumilus alleviates drought stress and increases metabolite accumulation in Glycyrrhiza uralensis Fisch. Environmental and Experimental Botany, 158, 99-106.
[44]	Yang B, Chen Y, Li X, Ren CG, Dai CC ( 2013) Research progress on endophyte-promoted plant nitrogen assimilation and metabolism. Acta Ecologica Sinica, 33, 2656-2664. (in Chinese with English abstract)
	[ 杨波, 陈晏, 李霞, 任承钢, 戴传超 ( 2013) 植物内生菌促进宿主氮吸收与代谢研究进展. 生态学报, 33, 2656-2664.]
[45]	Yang JH, Liu HX, Zhu GM, Pan YL, Xu LP, Guo JH ( 2008) Diversity analysis of antagonists from rice-associated bacteria and their application in biocontrol of rice diseases. Journal of Applied Microbiology, 104, 91-104.
[46]	Zhang FT, Xu T, Mao LY, Yan SY, Chen XW, Wu ZF, Chen R, Luo XD, Xie JK, Gao S ( 2016) Genome-wide analysis of Dongxiang wild rice (Oryza rufipogon Griff.) to investigate lost/acquired genes during rice domestication. BMC Plant Biology, 16, 103-114.
[47]	Zhao J, Liu T, Pan L, Jin BH, Zhao D, Chen C, Zhu YY, He XH ( 2015) Isolation and identification of root endophytic and rhizosphere bacteria of rice landraces in Yuanyang terrace, China. Chinese Journal of Applied Ecology, 26, 3737-3745. (in Chinese with English abstract)
	[ 赵娟, 刘涛, 潘磊, 靳百慧, 赵丹, 陈晨, 朱有勇, 何霞红 ( 2015) 元阳梯田地方水稻品种根部内生菌及根际微生物的分离与鉴定. 应用生态学报, 26, 3737-3745.]
[48]	Zhou Y, Yang P, Cui FL, Zhang FT, Luo XD, Xie JK ( 2016) Transcriptome analysis of salt stress responsiveness in the seedlings of Dongxiang wild rice (Oryza rufipogon Griff.). PLoS ONE, 11, e014624.

代表菌株			NCBI库中相似度最高菌种	基因登录号	序列相		分离菌株数				培养基分离菌株数
Representative strains			(登录号)	GenBank accession	似度		No. of strains				No. of strains from medium
			Closest relatives in NCBI	no.	Identity
			(GenBank accession no.)		(%)		根		茎	叶	NA	BPA	R2A	TSA	NA×10
							Root		Stem	Leaf	NA	BPA	R2A	TSA	NA×10
变形菌门 Proteobacteria
	泛菌属 Pantoea						5		1	6	3	2	3	2	2
		JXR10	Pantoea sp. (JX994157.1)	KP980595	100
		JXS8	Pantoea sp. (KJ733870.1)	KP980579	100
		JXL1	Pantoea sp. (KJ733870.1)	KP980566	100
	鞘氨醇单胞菌属 Sphingomonas						1		-	1	-	1	-	1	-
		JXR34	Sphingomonas yanoikuyae (KJ009425.1)	KP980605	100
		JXL6	Sphingomonas sanguinis (NR113637.1)	KP980568	100
	土壤杆菌属 Agrobacterium						-		3	1	1	-	1	-	2
		JXS10	Agrobacterium sp. (GQ169803.1)	KP980581	100
		JXL9	Agrobacterium sp. (KJ184856.1)	KP980570	100
	甲烷细菌属 Methylobacterium						1		1	1	-	1	-	1	1
		JXR16	Methylobacterium sp. (KM083547.1)	KP980599	100
		JXS9	Methylobacterium sp. (AB604650.1)	KP980580	100
		JXL16	Methylobacterium sp. (KM083546.1)	KP980572	100
	假单胞菌属 Pseudomonas						1		-	1	-	-	-	-	2
		JXR26	Pseudomonas sp. (HE978359.1)	KP980602	100
		JXL21	Pseudomonas fulva (AY741159.1)	KP980573	100
	寡养单胞菌属Stenotrophomonas						-		3	-	-	1	-	1	1
		JXS14	Stenotrophomonas maltophilia (JN571747.1)	KP980583	100
	根瘤菌属 Rhizobium						-		1	-	1	-	-	-	-
		JXS18	Rhizobium sp. (JN082742.1)	KP980584	100
	不动杆菌属 Acinetobacter						1		-	-	1	-	-	-	-
		JXR29	Acinetobacter oleivorans (KJ806471.1)	KP980603	100
放线菌门 Actinobacteria
	微小菌属 Microbacterium						1		8	10	5	3	6	2	3
		JXR14	Microbacterium trichothecenolyticum (KF953537.1)	KP980598	100
		JXS2	Microbacterium sp. (AB773218.1)	KP980577	100
		JXL7	Microbacterium sp. (EU741023.1)	KP980569	100
		JXL12	Microbacterium laevaniformans	KP980571	100
		JXL12	(KC252695.1)	KP980571	100
	短杆菌属 Curtobacterium						1		2	1	-	1	2	-	1
		JXR24	Curtobacterium citreum (KC329830.1)	KP980601	100
		JXS1	Curtobacterium luteum (JQ660182.1)	KP980576	97
		JXL30	Curtobacterium sp. (KC841444.1)	KP980575	100
	分支杆菌属 Mycobacterium						2		-	-	1	-	-	-	1
		JXR17	Mycobacterium sp. (AB605021.1)	KP980600	100
	节杆菌属 Arthrobacter						3		-	-	1	-	-	-	2
		JXR12	Arthrobacter phenanthrenivorans (KC934897.1)	KP980596	100
代表菌株				NCBI库中相似度最高菌种	基因登录号	序列相		分离株数			培养基分离株数
Representative strains				(登录号)	GenBank accession	似度		No. of strains			No. of strains from medium
				Closest relatives in NCBI	no.	Identity
				(GenBank accession no.)		(%)		根	茎	叶	NA	BPA	R2A	TSA		NA×10
								Root	Stem	Leaf	NA	BPA	R2A	TSA		NA×10
	雷弗森菌属 Leifsonia							3	-	-	-	-	1	-		2
		JXR6		Leifsonia sp. (KJ944083.1)	KP980592	100
	红球菌属 Rhodococcus							1	-	-	-	-	-	1		-
		JXR8		Rhodococcus equi (NR041910.1)	KP980594	100
厚壁菌门 Firmicutes
	类芽孢杆菌属 Paenibacillus							3	-		1	1	-	1		-
		JXR1		Paenibacillus agarexedens (KC355292.1)	KP980588	100
		JXR5		Paenibacillus sp. (KJ000069.1)	KP980591	99
		JXR30		Paenibacillus vulneris (KM272755.1)	KP980604	100
	芽孢杆菌属 Bacillus							7	9	10	8	8	1	2		7
		JXR2		Bacillus cereus (KF831402.1)	KP980589	100
		JXR3		Bacillus sp. (JX566646.1)	KP980590	100
		JXS6		Bacillus subtilis (KP229430.1)	KP980578	100
		JXS20		Bacillus sp. (KM555037.1)	KP980585	100
		JXS25		Bacillus mojavensis (HQ123468.1)	KP980587	97
		JXL24		Bacillus sp. (KP119613.1)	KP980574	100
	赖氨酸芽孢杆菌属 Lysinibacillus							4	-	1	1	-	2	1		1
		JXR13		Lysinibacillus sphaericus (KM070813.1)	KP980597	100
		JXL2		Lysinibacillus fusiformis (JN867122.1)	KP980567	100
总计 Total								34	28	32	23	18	16	12		25

代表菌株			NCBI库中相似度最高菌种	基因登录号	序列相		分离菌株数				培养基分离菌株数
Representative strains			(登录号)	GenBank accession	似度		No. of strains				No. of strains from medium
			Closest relatives in NCBI	no.	Identity
			(GenBank accession no.)		(%)		根		茎	叶	NA	BPA	R2A	TSA	NA×10
							Root		Stem	Leaf	NA	BPA	R2A	TSA	NA×10
变形菌门 Proteobacteria
	泛菌属 Pantoea						5		1	6	3	2	3	2	2
		JXR10	Pantoea sp. (JX994157.1)	KP980595	100
		JXS8	Pantoea sp. (KJ733870.1)	KP980579	100
		JXL1	Pantoea sp. (KJ733870.1)	KP980566	100
	鞘氨醇单胞菌属 Sphingomonas						1		-	1	-	1	-	1	-
		JXR34	Sphingomonas yanoikuyae (KJ009425.1)	KP980605	100
		JXL6	Sphingomonas sanguinis (NR113637.1)	KP980568	100
	土壤杆菌属 Agrobacterium						-		3	1	1	-	1	-	2
		JXS10	Agrobacterium sp. (GQ169803.1)	KP980581	100
		JXL9	Agrobacterium sp. (KJ184856.1)	KP980570	100
	甲烷细菌属 Methylobacterium						1		1	1	-	1	-	1	1
		JXR16	Methylobacterium sp. (KM083547.1)	KP980599	100
		JXS9	Methylobacterium sp. (AB604650.1)	KP980580	100
		JXL16	Methylobacterium sp. (KM083546.1)	KP980572	100
	假单胞菌属 Pseudomonas						1		-	1	-	-	-	-	2
		JXR26	Pseudomonas sp. (HE978359.1)	KP980602	100
		JXL21	Pseudomonas fulva (AY741159.1)	KP980573	100
	寡养单胞菌属Stenotrophomonas						-		3	-	-	1	-	1	1
		JXS14	Stenotrophomonas maltophilia (JN571747.1)	KP980583	100
	根瘤菌属 Rhizobium						-		1	-	1	-	-	-	-
		JXS18	Rhizobium sp. (JN082742.1)	KP980584	100
	不动杆菌属 Acinetobacter						1		-	-	1	-	-	-	-
		JXR29	Acinetobacter oleivorans (KJ806471.1)	KP980603	100
放线菌门 Actinobacteria
	微小菌属 Microbacterium						1		8	10	5	3	6	2	3
		JXR14	Microbacterium trichothecenolyticum (KF953537.1)	KP980598	100
		JXS2	Microbacterium sp. (AB773218.1)	KP980577	100
		JXL7	Microbacterium sp. (EU741023.1)	KP980569	100
		JXL12	Microbacterium laevaniformans	KP980571	100
		JXL12	(KC252695.1)	KP980571	100
	短杆菌属 Curtobacterium						1		2	1	-	1	2	-	1
		JXR24	Curtobacterium citreum (KC329830.1)	KP980601	100
		JXS1	Curtobacterium luteum (JQ660182.1)	KP980576	97
		JXL30	Curtobacterium sp. (KC841444.1)	KP980575	100
	分支杆菌属 Mycobacterium						2		-	-	1	-	-	-	1
		JXR17	Mycobacterium sp. (AB605021.1)	KP980600	100
	节杆菌属 Arthrobacter						3		-	-	1	-	-	-	2
		JXR12	Arthrobacter phenanthrenivorans (KC934897.1)	KP980596	100
代表菌株				NCBI库中相似度最高菌种	基因登录号	序列相		分离株数			培养基分离株数
Representative strains				(登录号)	GenBank accession	似度		No. of strains			No. of strains from medium
				Closest relatives in NCBI	no.	Identity
				(GenBank accession no.)		(%)		根	茎	叶	NA	BPA	R2A	TSA		NA×10
								Root	Stem	Leaf	NA	BPA	R2A	TSA		NA×10
	雷弗森菌属 Leifsonia							3	-	-	-	-	1	-		2
		JXR6		Leifsonia sp. (KJ944083.1)	KP980592	100
	红球菌属 Rhodococcus							1	-	-	-	-	-	1		-
		JXR8		Rhodococcus equi (NR041910.1)	KP980594	100
厚壁菌门 Firmicutes
	类芽孢杆菌属 Paenibacillus							3	-		1	1	-	1		-
		JXR1		Paenibacillus agarexedens (KC355292.1)	KP980588	100
		JXR5		Paenibacillus sp. (KJ000069.1)	KP980591	99
		JXR30		Paenibacillus vulneris (KM272755.1)	KP980604	100
	芽孢杆菌属 Bacillus							7	9	10	8	8	1	2		7
		JXR2		Bacillus cereus (KF831402.1)	KP980589	100
		JXR3		Bacillus sp. (JX566646.1)	KP980590	100
		JXS6		Bacillus subtilis (KP229430.1)	KP980578	100
		JXS20		Bacillus sp. (KM555037.1)	KP980585	100
		JXS25		Bacillus mojavensis (HQ123468.1)	KP980587	97
		JXL24		Bacillus sp. (KP119613.1)	KP980574	100
	赖氨酸芽孢杆菌属 Lysinibacillus							4	-	1	1	-	2	1		1
		JXR13		Lysinibacillus sphaericus (KM070813.1)	KP980597	100
		JXL2		Lysinibacillus fusiformis (JN867122.1)	KP980567	100
总计 Total								34	28	32	23	18	16	12		25

组织名称 Tissue	细菌属数 Genus	多样性指数 Shannon- Wiener index	优势度指数 Simpson index	均匀度指数 Pielou index
根 Root	14	2.52	0.88	0.72
茎 Stem	8	1.81	0.80	0.54
叶 Leaf	9	2.09	0.74	0.60

组织名称 Tissue	细菌属数 Genus	多样性指数 Shannon- Wiener index	优势度指数 Simpson index	均匀度指数 Pielou index
根 Root	14	2.52	0.88	0.72
茎 Stem	8	1.81	0.80	0.54
叶 Leaf	9	2.09	0.74	0.60

东乡野生稻可培养内生细菌群落组成及多样性

Community composition and diversity of cultivable endophytic bacteria isolated from Dongxiang wild rice

RichHTML

PDF (PC)

可视化

被引次数

摘要/Abstract

引用本文

使用本文

图/表 5

参考文献 48

相关文章 15

编辑推荐

Metrics

本文评价

[1]	王腾, 李纯厚, 王广华, 赵金发, 石娟, 谢宏宇, 刘永, 刘玉. 西沙群岛七连屿珊瑚礁鱼类的物种组成与演替[J]. 生物多样性, 2024, 32(6): 23481-.
[2]	艾妍雨, 胡海霞, 沈婷, 莫雨轩, 杞金华, 宋亮. 附生维管植物多样性及其与宿主特征的相关性: 以哀牢山中山湿性常绿阔叶林为例[J]. 生物多样性, 2024, 32(5): 24072-.
[3]	吕燕文, 王子韵, 肖钰, 何梓晗, 吴超, 胡新生. 谱系分选理论与检测方法的研究进展[J]. 生物多样性, 2024, 32(4): 23400-.
[4]	吴琪, 张晓青, 杨雨婷, 周艺博, 马毅, 许大明, 斯幸峰, 王健. 浙江钱江源-百山祖国家公园庆元片区叶附生苔多样性及其时空变化[J]. 生物多样性, 2024, 32(4): 24010-.
[5]	曹可欣, 王敬雯, 郑国, 武鹏峰, 李英滨, 崔淑艳. 降水格局改变及氮沉降对北方典型草原土壤线虫多样性的影响[J]. 生物多样性, 2024, 32(3): 23491-.
[6]	张楚然, 李生发, 李逢昌, 唐志忠, 刘辉燕, 王丽红, 顾荣, 邓云, 张志明, 林露湘. 云南鸡足山亚热带半湿润常绿阔叶林20 ha动态监测样地木本植物生境关联与群落数量分类[J]. 生物多样性, 2024, 32(1): 23393-.
[7]	李庆多, 栗冬梅. 全球蝙蝠巴尔通体流行状况分析[J]. 生物多样性, 2023, 31(9): 23166-.
[8]	韩赟, 迟晓峰, 余静雅, 丁旭洁, 陈世龙, 张发起. 青海野生维管植物名录[J]. 生物多样性, 2023, 31(9): 23280-.
[9]	杜红. “物种”与“个体”: 究竟谁是生物多样性保护的恰当对象?[J]. 生物多样性, 2023, 31(8): 23140-.
[10]	韦毅刚, 温放, 辛子兵, 符龙飞. 广西野生维管植物名录[J]. 生物多样性, 2023, 31(6): 23078-.
[11]	邵雯雯, 范国祯, 何知舟, 宋志平. 多地同质园实验揭示普通野生稻的表型可塑性与本地适应性[J]. 生物多样性, 2023, 31(3): 22311-.
[12]	黄雨菲, 路春燕, 贾明明, 王自立, 苏越, 苏艳琳. 基于无人机影像与面向对象-深度学习的滨海湿地植物物种分类[J]. 生物多样性, 2023, 31(3): 22411-.
[13]	林魏巍, 田呈明, 熊典广, 刘伟航, 热依汗古丽·斯地克, 梁英梅. 新疆杨树人工林中蜘蛛群落多样性及其影响因素[J]. 生物多样性, 2023, 31(3): 22493-.
[14]	李治中, 彭帅, 王青锋, 李伟, 梁士楚, 陈进明. 中国海菜花属植物隐种多样性[J]. 生物多样性, 2023, 31(2): 22394-.
[15]	宋会银, 胡征宇, 刘国祥. 绿藻门小球藻科的分类学研究进展[J]. 生物多样性, 2023, 31(2): 22083-.