生物多样性 ›› 2015, Vol. 23 ›› Issue (5): 649-657. DOI: 10.17520/biods.2015032
所属专题: 森林动态监测样地专题
赵爱花1,2, 杜晓军1,*(), 臧婧1, 张守仁1, 焦志华3
收稿日期:
2015-02-10
接受日期:
2015-02-10
出版日期:
2015-09-20
发布日期:
2015-10-12
通讯作者:
杜晓军
基金资助:
Aihua Zhao1,2, Xiaojun Du1,*(), Jing Zang1, Shouren Zhang1, Zhihua Jiao3
Received:
2015-02-10
Accepted:
2015-02-10
Online:
2015-09-20
Published:
2015-10-12
Contact:
Du Xiaojun
摘要:
土壤微生物在森林生态系统中起着重要作用。高通量测序方法的出现为进一步认识土壤微生物提供了契机。本文利用Illumina Miseq高通量测序技术对宝天曼森林土壤的细菌多样性进行了初步研究。结果显示: 在31个采样点内, 随着采样点增加, 检测出不同分类水平的土壤细菌类群也在增多, 当采样点达到31个时, 检测出的土壤细菌类群达到45门163纲319目495科785属和42,632个OTU; 31个土壤样品中所检测出的细菌类群平均有34.2门114.7纲215.2目323.7科446.6属5,924.7个OTU, 其中门、纲、目分类水平上的优势类群(所占比例)分别为变形菌门(Proteobacteria)(38.30%)、α-变形菌纲(α-Proteobacteria)(18.08%)、根瘤菌目(Rhizobiales)(10.62%)。这些初步研究结果表明在一定程度上宝天曼森林土壤有较高的细菌多样性水平, 为进一步认识森林土壤细菌多样性与植物多样性关系等奠定了基础。
赵爱花, 杜晓军, 臧婧, 张守仁, 焦志华 (2015) 宝天曼落叶阔叶林土壤细菌多样性. 生物多样性, 23, 649-657. DOI: 10.17520/biods.2015032.
Aihua Zhao, Xiaojun Du, Jing Zang, Shouren Zhang, Zhihua Jiao (2015) Soil bacterial diversity in the Baotianman deciduous broad-leaved forest. Biodiversity Science, 23, 649-657. DOI: 10.17520/biods.2015032.
样品编号 Sample identity | 原始序列数 Original sequence number | 原始OTU数 Original OTU number | 标准化序列数 Even sequence number | 标准化后OTU数 Even OTU number |
---|---|---|---|---|
0101 | 37,569 | 7,148 | 36,000 | 6,979 |
0105 | 39,949 | 5,671 | 36,000 | 5,320 |
0203 | 39,698 | 5,661 | 36,000 | 5,327 |
0218 | 39,337 | 5,579 | 36,000 | 5,320 |
0306 | 39,281 | 6,396 | 36,000 | 6,097 |
0318 | 39,760 | 6,263 | 36,000 | 5,906 |
0411 | 39,756 | 6,008 | 36,000 | 5,672 |
0606 | 39,636 | 6,642 | 36,000 | 6,286 |
0701 | 39,945 | 6,059 | 36,000 | 5,694 |
0819 | 39,864 | 6,324 | 36,000 | 5,933 |
0913 | 39,827 | 5,275 | 36,000 | 4,971 |
0919 | 39,720 | 5,958 | 36,000 | 5,645 |
1002 | 39,844 | 5,486 | 36,000 | 5,175 |
1006 | 39,551 | 6,658 | 36,000 | 6,322 |
1018 | 39,711 | 5,526 | 36,000 | 5,184 |
1104 | 39,746 | 6,380 | 36,000 | 6,036 |
1210 | 39,569 | 6,255 | 36,000 | 5,933 |
1212 | 39,659 | 6,016 | 36,000 | 5,691 |
1318 | 39,809 | 6,706 | 36,000 | 6,326 |
1405 | 39,825 | 5,910 | 36,000 | 5,547 |
1510 | 39,379 | 5,650 | 36,000 | 5,378 |
1512 | 37,588 | 5,555 | 36,000 | 5,413 |
1607 | 39,739 | 6,338 | 36,000 | 5,981 |
1615 | 39,212 | 6,246 | 36,000 | 5,978 |
1618 | 39,578 | 6,552 | 36,000 | 6,200 |
1704 | 39,495 | 6,405 | 36,000 | 6,024 |
1718 | 39,130 | 6,910 | 36,000 | 6,564 |
1808 | 39,739 | 6,833 | 36,000 | 6,498 |
1814 | 39,866 | 6,850 | 36,000 | 6,501 |
1902 | 39,155 | 7,283 | 36,000 | 6,920 |
1913 | 38,882 | 7,145 | 36,000 | 6,844 |
表1 样品序列数及OTU数
Table 1 Sequence numbers and OTU numbers of individual samples
样品编号 Sample identity | 原始序列数 Original sequence number | 原始OTU数 Original OTU number | 标准化序列数 Even sequence number | 标准化后OTU数 Even OTU number |
---|---|---|---|---|
0101 | 37,569 | 7,148 | 36,000 | 6,979 |
0105 | 39,949 | 5,671 | 36,000 | 5,320 |
0203 | 39,698 | 5,661 | 36,000 | 5,327 |
0218 | 39,337 | 5,579 | 36,000 | 5,320 |
0306 | 39,281 | 6,396 | 36,000 | 6,097 |
0318 | 39,760 | 6,263 | 36,000 | 5,906 |
0411 | 39,756 | 6,008 | 36,000 | 5,672 |
0606 | 39,636 | 6,642 | 36,000 | 6,286 |
0701 | 39,945 | 6,059 | 36,000 | 5,694 |
0819 | 39,864 | 6,324 | 36,000 | 5,933 |
0913 | 39,827 | 5,275 | 36,000 | 4,971 |
0919 | 39,720 | 5,958 | 36,000 | 5,645 |
1002 | 39,844 | 5,486 | 36,000 | 5,175 |
1006 | 39,551 | 6,658 | 36,000 | 6,322 |
1018 | 39,711 | 5,526 | 36,000 | 5,184 |
1104 | 39,746 | 6,380 | 36,000 | 6,036 |
1210 | 39,569 | 6,255 | 36,000 | 5,933 |
1212 | 39,659 | 6,016 | 36,000 | 5,691 |
1318 | 39,809 | 6,706 | 36,000 | 6,326 |
1405 | 39,825 | 5,910 | 36,000 | 5,547 |
1510 | 39,379 | 5,650 | 36,000 | 5,378 |
1512 | 37,588 | 5,555 | 36,000 | 5,413 |
1607 | 39,739 | 6,338 | 36,000 | 5,981 |
1615 | 39,212 | 6,246 | 36,000 | 5,978 |
1618 | 39,578 | 6,552 | 36,000 | 6,200 |
1704 | 39,495 | 6,405 | 36,000 | 6,024 |
1718 | 39,130 | 6,910 | 36,000 | 6,564 |
1808 | 39,739 | 6,833 | 36,000 | 6,498 |
1814 | 39,866 | 6,850 | 36,000 | 6,501 |
1902 | 39,155 | 7,283 | 36,000 | 6,920 |
1913 | 38,882 | 7,145 | 36,000 | 6,844 |
图2 宝天曼森林动态监测样地31个土壤样品中检测到的不同分类水平上的细菌数量
Fig. 2 The quantity of bacteria at different taxonomic levels in the 31 soil samples in the Baotianman Forest Dynamics Plot
图3 宝天曼森林动态监测样地31个土壤样品中检测到的不同分类水平上细菌类群数随采样点数增加的稀疏曲线
Fig. 3 Rarefaction curves of the observed bacterial quantity at different taxonomic levels varying with number of samples in the Baotianman Forest Dynamics Plot
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