生物多样性 ›› 2000, Vol. 08 ›› Issue (1): 103-105.  DOI: 10.17520/biods.2000014

• 论文 • 上一篇    下一篇

用PCR及RFLP方法分析支原体与植原体的同源性

邓兆群,胡勤学,柳晟,王瑶,林木兰   

  1. 1 ( 湖北医科大学微生物及免疫学教研室,  武汉 430071)
    2 ( 中国科学院武汉病毒研究所,  武汉 430071)
  • 收稿日期:1999-05-11 修回日期:1999-08-20 出版日期:2000-02-20 发布日期:2000-02-20
  • 通讯作者: 林木兰

A homologous comparison between mycoplasma and phytoplasma using 16S rDNA PCR amplification and RFLP analysis

DENG Zhao-Qun, HU Qin-Xue, LIU Sheng, WANG Yao, LIN Mu-Lan   

  1. 1) Wuhan Institute of Virology , The Chinese Academy of Sciences , Wuhan  430071
    2) Hubei Medical University , Wuhan  430071
  • Received:1999-05-11 Revised:1999-08-20 Online:2000-02-20 Published:2000-02-20
  • Contact: LIN Mu-Lan

摘要:

将猪鼻支原体和泡桐丛枝病植原体的16S rDNA进行PCR扩增,分别得到一条1 kb左右的扩增片段。PCR扩增产物用限制性内切酶EcoRⅠ、HindⅢ、BamHⅠ、SalⅠ和SmaⅠ进行RFLP(限制性片段长度多态性)分析,发现用RFLP分析猪鼻支原体和泡桐丛枝病植原体16S rDNA序列同源性的相关系数为0.72。

关键词: 物种多样性, 鸟类物种丰富度, 兽类物种丰富, 动物地理, 自然保护区

Abstract16S rDNA from mycoplasma and phytoplasma was amplified by PCR , and a band was detected at about 1 kb. DNA products were digested with restriction enzymes EcoR Ⅰ, Hind Ⅲ, Bam H Ⅰ, Sal Ⅰ and Sma Ⅰ, and then were analyzed using RFLP. Similarity coefficient derived from RFL P analysis of mycoplasma and phytoplasma is 0. 72

Key words: species diversity, avian species richness, mam malian species richness, zoogeography, nature reserve