生物多样性 ›› 2019, Vol. 27 ›› Issue (12): 1320-1329.DOI: 10.17520/biods.2019219

• 研究报告: 微生物多样性 • 上一篇    下一篇

东乡野生稻可培养内生细菌群落组成及多样性

陈志远1,刘珺1,杨星鹏1,刘梦1,汪涯2,张志斌1,*(),朱笃1,2,*   

  1. 1 江西师范大学生命科学学院, 江西省亚热带植物资源保护与利用重点实验室, 南昌 330022
    2 江西科技师范大学生命科学学院, 江西省生物加工过程重点实验室, 南昌 330013
  • 收稿日期:2019-07-07 接受日期:2019-10-20 出版日期:2019-12-20 发布日期:2020-02-22
  • 通讯作者: 张志斌,朱笃
  • 基金资助:
    国家自然科学基金(31760160);江西省自然科学基金(20171BAB204009);江西省自然科学基金(20181BAB215044);江西师范大学研究生创新项目(YJS2018080);江西师范大学研究生创新项目(YC2019-S121)

Community composition and diversity of cultivable endophytic bacteria isolated from Dongxiang wild rice

Zhiyuan Chen1,Jun Liu1,Xingpeng Yang1,Meng Liu1,Ya Wang2,Zhibin Zhang1,*(),Du Zhu1,2,*   

  1. 1 Key Laboratory of Protection and Utilization of Subtropical Plant Resources of Jiangxi Province, College of Life Sciences, Jiangxi Normal University, Nanchang 330022
    2 Key Laboratory of Bioprocess Engineering of Jiangxi Province, College of Life Sciences, Jiangxi Science and Technology Normal University, Nanchang 330013
  • Received:2019-07-07 Accepted:2019-10-20 Online:2019-12-20 Published:2020-02-22
  • Contact: Zhang Zhibin,Zhu Du

摘要:

内生细菌对宿主植物生长发育和生理代谢具有重要影响, 揭示其群落组成和多样性具有重要的生物学和生态学意义。本研究采用可培养法从东乡野生稻(Oryza rufipogon)不同组织中分离获得94株内生细菌, 基于16S rRNA基因序列比对和系统发育分析将其归属于变形菌门、放线菌门和厚壁菌门3门14科17属, 其中芽孢杆菌属(Bacillus)和微小菌属(Microbacterium)为优势属, 分别占总株数的27.7%和20.2%。不同组织中内生细菌的分布和多样性存在差异, 根中内生细菌数量最多(34株, 36.2%), 叶次之(32株, 34.0%), 茎最少(28株, 29.8%); 根中内生细菌Shannon-Wiener多样性指数(H′ = 2.52)、Simpson优势度指数(D = 0.88)和Pielou均匀度指数(E = 0.72)均高于茎与叶, 根和茎的Jaccard相似性系数(C = 0.29)低于根和叶(C = 0.47)以及茎和叶(C = 0.45)。研究结果表明, 东乡野生稻内生细菌具有丰富的物种多样性, 并且不同组织部位的内生细菌组成存在差异, 根中内生细菌多样性最丰富。

关键词: 野生稻, 植物内生菌, 分类, 系统发育, 组成分析

Abstract:

Endophytic bacteria are essential to the growth and metabolism of their host plants. To better understand the community structure and diversity of endophytic bacteria on plants, we isolated 94 strains of endophytic bacteria from the roots, stems and leaves of Dongxiang wild rice (Oryza rufipogon). Subsequent 16S rRNA sequence alignments and phylogenetic analyses revealed that the 94 strains could be categorized into 17 genera of 14 families in 3 phyla (Proteobacteria, Actinobacteria, and Firmicutes).Bacillus and Microbacterium, accounting for 27.7% and 20.2% of the diversity, respectively, were the two most dominant genera. The distribution and diversity of endophytic bacteria differed across different tissues of Dongxiang wild rice: the roots contained the most endophytic bacteria strains (n = 34, 36.2%), followed by leaves (n = 32, 34.0%), and stems (n = 28, 29.8%). Shannon-Wiener diversity index (H′ = 2.52), Simpson dominance index (D = 0.88) and Pielou evenness index (E = 0.72) were higher in the roots than in the stems or leaves. Meanwhile, the Jaccard similarity index between roots and stems (C = 0.29) was lower than that between roots and leaves (C = 0.47), or that between stems and leaves (C = 0.45). Our efforts to uncover the diversity of endophytic bacteria in Dongxiang wild rice confirms that roots contain the most diverse strains but that the composition of endophytes varies across tissues.

Key words: Oryza rufipogon, endophytes, classification, phylogeny, composition analysis