生物多样性 ›› 2017, Vol. 25 ›› Issue (4): 409-417.doi: 10.17520/biods.2016331

• 研究报告: 动物多样性 • 上一篇    下一篇

白洋淀湿地蝗虫多样性调查及DNA条形码应用研究

郝金凤, 张晓红, 王昱淞, 刘金林, 智永超, 李新江*()   

  1. 河北大学生命科学学院河北省动物系统学与应用重点实验室, 河北保定 071002
  • 收稿日期:2016-11-22 接受日期:2017-04-10 出版日期:2017-04-20
  • 通讯作者: 李新江 E-mail:hbulxj@163.com
  • 基金项目:
    基金项目: 河北省高等学校科学技术研究项目(QN2014017)

Diversity investigation and application of DNA barcoding of Acridoidea from Baiyangdian Wetland

Jinfeng Hao, Xiaohong Zhang, Yusong Wang, Jinlin Liu, Yongchao Zhi, Xinjiang Li*()   

  1. The Key Laboratory of Zoological Systematics and Application, College of Life Sciences, Hebei University, Baoding, Hebei 071002
  • Received:2016-11-22 Accepted:2017-04-10 Online:2017-04-20
  • Contact: Li Xinjiang E-mail:hbulxj@163.com

为了解白洋淀湿地蝗虫的物种多样性及其分布情况, 探究DNA条形码技术在物种鉴定方面的可行性, 本研究对白洋淀湿地的蝗虫进行了采集调查。采用自行设计的cox1基因引物, 本研究共扩增得到21种蝗虫的cox1序列97条, 并利用构建NJ系统发育树法、计算遗传距离的ABGD法以及序列差异阈值分类的MOTU法对已得到的序列连同GenBank下载的10种25条cox1序列进行了比较分析。结果表明: 白洋淀湿地蝗总科昆虫共有6科23属34种, 包括1个新记录属, 即异爪蝗属(Euchorthippus), 和3个新记录种, 即素色异爪蝗(E. unicolor)、柳枝负蝗(Atractomorpha psittacina)和日本稻蝗(Oxya japonica); 蝗虫在白洋淀湿地主要分布在农田及其周围、堤坝及其周围以及淀边草丛; DNA条形码技术能够在种级阶元对蝗总科物种进行良好地解析, 但物种鉴定时, 不能完全脱离形态学方法。

关键词: 直翅目, 蝗总科, 白洋淀湿地, 多样性, DNA条形码

Both the species diversity and distribution pattern of the superfamily Acridoidea of the suborder Caelifera have important contributions to understanding the local biodiversity of the Baiyangdian Wetland. This research tries to study the species diversity and distribution pattern of the superfamily Acridoidea within the Baiyangdian Wetland and test the feasibility of DNA barcoding in species identification for this superfamily. Sequences of the cox1 gene were obtained from 97 individuals of 21 species of the superfamily Acridoidea. Phylogenetic, genetic distance and sequence difference threshold analyses using the Neighbor Joining (NJ), Automatic Barcode Gap Discovery (ABGD) and Molecular Defined Operational Taxonomic Units (MOTU) methods, respectively, were performed for these and the 25 additional sequences of 10 species downloaded from GenBank. The results indicate that there are 34 species, 23 genera, and 6 families of the superfamily Acridoidea insects around the farmland, dam, and grassland of the Baiyangdian Wetland, including a new-record genus, Euchorthippus, and three new-record species, Euchorthippus unicolor, Atractomorpha psittacina and Oxya japonica. The DNA barcoding technology therefore is very efficient and helpful for identifying the species of the superfamily Acridoidea, although the morphological approach is still playing a key role in the species identifications.

Key words: Orthoptera, Acridoidea, Baiyangdian Wetland, diversity, DNA barcoding

图1

白洋淀湿地蝗虫采集点分布图"

表1

白洋淀湿地蝗总科昆虫名录及标本采集信息"

科名
Family
属名
Genus
种名
Species
采集地点
Collection location
采集时间
Collection time
癞蝗科 Pamphagidae 笨蝗属 Haplotropis 笨蝗 Haplotropis brunneriana
锥头蝗科
Pyrgomorphidae
负蝗属 Atractomorpha 短额负蝗 Atractomorpha sinensis 寨里 Zhaili
崔公堤 Cuigongdi
大王镇 Dawangzhen
大树刘庄 Dashuliuzhuang
2011.8
2014.9
2015.8
2015.9
柳枝负蝗 Atractomorpha psittacina 宋庄 Songzhuang
杨庄子 Yangzhuangzi
赵北口 Zhaobeikou
2015.8
2011.8
2014.9
令箭负蝗 Atractomorpha sagittaris 南曲堤 Nanqudi
大树刘庄 Dashuliuzhuang
端村 Duancun
2011.8
2015.9
2014.9
长额负蝗 Atractomorpha lata 圈头 Quantou
崔公堤 Cuigongdi
杨庄子 Yangzhuangzi
宋庄 Songzhuang
2011.8
2014.9
2011.8
2015.8
斑腿蝗科 Catantopidae 稻蝗属 Oxya 日本稻蝗 Oxya japonica 寨里 Zhaili
大王镇 Dawangzhen
端村 Duancun
2011.8
2015.8
2014.9
中华稻蝗 Oxya chinensis 圈头 Quantou
南曲堤 Nanqudi
大树刘庄 Dashuliuzhuang
2011.8
2011.8
2015.9
蔗蝗属 Hieroglyphus 斑角蔗蝗 Hieroglyphus annulicornis
棉蝗属 Chondracris 棉蝗 Chondracris rosea rosea
黄脊蝗属 Patanga 日本黄脊蝗 Patanga japonica
星翅蝗属 Calliptamus 短星翅蝗 Calliptamus abbreviatus 杨庄子 Yangzhuangzi
赵北口 Zhaobeikou
大王镇 Dawangzhen
2011.8
2014.9
2015.8
素木蝗属 Shirakiacris 长翅素木蝗 Shirakiacris shirakii 南曲堤 Nanqudi
大树刘庄 Dashuliuzhuang
赵北口 Zhaobeikou
2011.8
2015.9
2014.9
斑翅蝗科 Oedipodidae 飞蝗属 Locusta 东亚飞蝗
Locusta migratoria manilensis
崔公堤 Cuigongdi
大王镇 Dawangzhen
赵北口 Zhaobeikou
宋庄 Songzhuang
2014.9
2015.8
2014.9
2015.8
车蝗属 Gastrimargus 云斑车蝗 Gastrimargus marmoratus
尖翅蝗属 Epacromius 大垫尖翅蝗 Epacromius coerulipes 端村 Duancun
赵北口 Zhaobeikou
崔公堤 Cuigongdi
2014.9
2011.8
2014.9
小车蝗属 Oedaleus 黄胫小车蝗
Oedaleus infernalis infernalis
大王镇 Dawangzhen
端村 Duancun
南曲堤 Nanqudi
崔公堤 Cuigongdi
2015.8
2014.9
2011.8
2014.9
亚洲小车蝗 Oedaleus asiaticus 杨庄子 Yangzhuangzi
南曲堤 Nanqudi
崔公堤 Cuigongdi
赵北口 Zhaobeikou
端村 Duancun
2011.8
2011.8
2014.9
2014.9
2014.9
赤翅蝗属 Celes 大赤翅蝗 Celes skalozubovi akitanus
疣蝗属 Trilophidia 疣蝗 Trilophidia annulata 崔公堤 Cuigongdi
赵北口 Zhaobeikou
杨庄子 Yangzhuangzi
2014.9
2014.9
2011.8
绿纹蝗属 Aiolopus 花胫绿纹蝗 Aiolopus tamulus 崔公堤 Cuigongdi
大王镇 Dawangzhen
赵北口 Zhaobeikou
杨庄子 Yangzhuangzi
2014.9
2015.8
2014.9
2011.8
异痂蝗属 Bryodemella 轮纹异痂蝗 Bryodemella tuberculatum dilutum
网翅蝗科
Arcypteridae
曲背蝗属 Pararcyptera 宽翅曲背蝗
Pararcyptera microptera meridionalis
网翅蝗属 Arcyptera 隆额网翅蝗 Arcyptera coreana
雏蝗属 Chorthippus 黑翅雏蝗 Chorthippus aethalinus
小翅雏蝗 Chorthippus fallax
北方雏蝗 Chorthippus hammarstroemi
狭翅雏蝗 Chorthippus dubius
白纹雏蝗 Chorthippus albonemus
华北雏蝗
Chorthippus brunneus huabeiensis
东方雏蝗 Chorthippus intermedius
异爪蝗属
Euchorthippus
素色异爪蝗 Euchorthippus unicolor 寨里 Zhaili
崔公堤 Cuigongdi
大王镇 Dawangzhen
2011.8
2014.9
2015.8
剑角蝗科
Acrididae
鸣蝗属
Mongolotettix
条纹鸣蝗
Mongolotettix japonicus vittatus
剑角蝗属 Acrida 中华剑角蝗 Acrida cinerea 南曲堤 Nanqudi
大树刘庄 Dashuliuzhuang
大王镇 Dawangzhen
崔公堤 Cuigongdi
寨里 Zhaili
2011.8
2015.9
2015.8
2014.9
2011.8
戛蝗属 Gonista 二色戛蝗 Gonista bicolor 崔公堤 Cuigongdi
大王镇 Dawangzhen
寨里 Zhaili
2014.9
2015.8
2011.8

图2

基于cox1序列构建的白洋淀湿地蝗总科昆虫NJ系统发育树、ABGD和MOTU聚类结果"

图3

以0.001-0.1的先验值区间对122个样本ABGD划分结果"

图4

0-100的分子序列差异截取值与可操作分类单元数目的关系"

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