Biodiv Sci ›› 2013, Vol. 21 ›› Issue (5): 616-627. DOI: 10.3724/SP.J.1003.2013.12059
Special Issue: 物种形成与系统进化
• Orginal Article • Previous Articles Next Articles
Received:
2013-03-07
Accepted:
2013-09-04
Online:
2013-09-20
Published:
2013-10-08
Contact:
Pei Nancai
Nancai Pei,Bufeng Chen. DNA barcoding of life: a classification of uses according to function and scale after ten years of development[J]. Biodiv Sci, 2013, 21(5): 616-627.
类别 Item | 片段名称 Barcode | 引物 Primer sequence (5′- 3′) | 来源 Source | 序列长度 Size (bp) | 参考文献 References |
---|---|---|---|---|---|
动物 Animal | COI | 正向: GGTCAACAAATCATAAAGATATTGG 反向: TAAACTTCAGGGTGACCAAAAAATCA | 线粒体基因编码区 Coding region in mitochondrial gene | ~650 | Hebert et al., 2003a |
植物 Plant | rbcL matK trnH-psbA ITS trnL-F rps4 | 正向: ATGTCACCACAAACAGAGACTAAAGC 反向: GTYAAATCAAGTCCACCYCG 正向: CGTACAGTACTTTTGTGTTTACGAG 反向: ACCCAGTCCATCTGGAAATCTTGGTTC matK472F: CCCRTYCATCTGGAAATCTTGGTTC matK1248R: GCTRTRATAATGAGAAAGATTTCTGC trnH端: CGCGCATGGTGGATTCACAATCC psbA端: GTTATGCATGAACGTAATGCTC 正向: AGAAGTCGTAACAAGGTTTCCGTAGG 反向: TCCTCCGCTTATTGATATGC 正向: AGAAGTCGTAACAAGGTTTCCGTAGG或GGAAGGAGAAGTCGTAACAAGG; (裸子植物正向: GTCCACTGAACCTTATCATTTAG) 反向: TCCTCCGCTTATTGATATGC GTAAAACGACGGCCAGT CA-GGAA-ACAGCTATGAC | 叶绿体基因编码区 Coding region in chloroplast gene 叶绿体基因编码区 Coding region in chloroplast gene 叶绿体非编码基因间隔区 Non-coding intergenic region in chloroplast gene 核基因非编码基因间隔区 Non-coding intergenic region in nuclear gene 叶绿体非编码基因间隔区 Non-coding intergenic region in chloroplast gene 叶绿体基因编码区 Coding region in chloroplast gene | ~550 ~850 ~776 280-750 ~500(由多个较短序列组成) 350-500 700-900 | CBOL Plant Working Group, 2009; Kress & Erickson, 2007 Kim KJ, unpublished primers (http://www.korea.edu/academics/01co_04.php) Yu et al., 2011 Kress & Erickson, 2007 China Plant BOL Group, 2011 de Groot et al., 2011 Nadot et al., 1994 刘艳等, 2011 |
微生物 Microbe | ITS 18S rDNA | 正向: TCCTCCGCTTATTGATATGC 反向: GGAAGTAAAAGTCGTAACAAGG 正向: AGATTAAGCCATGCATGTCT 反向: GATCCTTCCGCAGGTTCACCTAC | 核基因非编码基因间隔区 Non-coding intergenic region in nuclear gene 核基因编码区 Coding region in nuclear gene | 400-660 1,600 | Seifert, 2009; Schoch et al., 2012; 张宇和郭良栋, 2012 Pawlowski et al., 2012 |
Table 1 A list of currently used DNA barcodes of life
类别 Item | 片段名称 Barcode | 引物 Primer sequence (5′- 3′) | 来源 Source | 序列长度 Size (bp) | 参考文献 References |
---|---|---|---|---|---|
动物 Animal | COI | 正向: GGTCAACAAATCATAAAGATATTGG 反向: TAAACTTCAGGGTGACCAAAAAATCA | 线粒体基因编码区 Coding region in mitochondrial gene | ~650 | Hebert et al., 2003a |
植物 Plant | rbcL matK trnH-psbA ITS trnL-F rps4 | 正向: ATGTCACCACAAACAGAGACTAAAGC 反向: GTYAAATCAAGTCCACCYCG 正向: CGTACAGTACTTTTGTGTTTACGAG 反向: ACCCAGTCCATCTGGAAATCTTGGTTC matK472F: CCCRTYCATCTGGAAATCTTGGTTC matK1248R: GCTRTRATAATGAGAAAGATTTCTGC trnH端: CGCGCATGGTGGATTCACAATCC psbA端: GTTATGCATGAACGTAATGCTC 正向: AGAAGTCGTAACAAGGTTTCCGTAGG 反向: TCCTCCGCTTATTGATATGC 正向: AGAAGTCGTAACAAGGTTTCCGTAGG或GGAAGGAGAAGTCGTAACAAGG; (裸子植物正向: GTCCACTGAACCTTATCATTTAG) 反向: TCCTCCGCTTATTGATATGC GTAAAACGACGGCCAGT CA-GGAA-ACAGCTATGAC | 叶绿体基因编码区 Coding region in chloroplast gene 叶绿体基因编码区 Coding region in chloroplast gene 叶绿体非编码基因间隔区 Non-coding intergenic region in chloroplast gene 核基因非编码基因间隔区 Non-coding intergenic region in nuclear gene 叶绿体非编码基因间隔区 Non-coding intergenic region in chloroplast gene 叶绿体基因编码区 Coding region in chloroplast gene | ~550 ~850 ~776 280-750 ~500(由多个较短序列组成) 350-500 700-900 | CBOL Plant Working Group, 2009; Kress & Erickson, 2007 Kim KJ, unpublished primers (http://www.korea.edu/academics/01co_04.php) Yu et al., 2011 Kress & Erickson, 2007 China Plant BOL Group, 2011 de Groot et al., 2011 Nadot et al., 1994 刘艳等, 2011 |
微生物 Microbe | ITS 18S rDNA | 正向: TCCTCCGCTTATTGATATGC 反向: GGAAGTAAAAGTCGTAACAAGG 正向: AGATTAAGCCATGCATGTCT 反向: GATCCTTCCGCAGGTTCACCTAC | 核基因非编码基因间隔区 Non-coding intergenic region in nuclear gene 核基因编码区 Coding region in nuclear gene | 400-660 1,600 | Seifert, 2009; Schoch et al., 2012; 张宇和郭良栋, 2012 Pawlowski et al., 2012 |
Fig. 1 A graphic representation of DNA barcoding technological process (adopted from Janzen et al., 2009 and Prof. Paul Hebert’s PPT slides from CBOL Meeting in Beijing, May 2009)
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