Genetic diversity in fourProcambarus clarkii populations in the lower reaches of the Yangtze River

doi:10.3724/SP.J.1003.2009.09017

Abstract

Abstract:

To estimate genetic variation and provide genetic information for resource protection and breeding efforts, individuals of the invasive red swamp crayfish (Procambarus clarkii) from four populations along the lower reaches of the Yangtze River (Nanjing, Xuyi, Hefei and Nanchang) were characterized using 17 microsatellite loci. In these populations, the mean observed heterozygosity (H_o), mean expected heterozygosity (H_e), and mean polymorphism information content (PIC) ranged from 0.4322 to 0.4826, from 0.4024 to 0.6121, and from 0.3408 to 0.5624, respectively, indicating a medium level of genetic diversity. The highest and lowest genetic diversity were found in the Nanjing population and Hefei population, respectively. Gene flow (N_m) ranged from 1.3729 to 5.9161, and population differentiation (F_st) ranged from 0.0405 to 0.1540. Cluster analysis using the UPGMA method showed that the Nanjing and Nanchang populations clustered first, and then clustered with the Xuyi population, and finally all groups together clustered with the Hefei population.

Key words: Procambarus clarkii, microsatellite, genetic breeding, genetic variation

Changzhong Wang, Zhong Li, Hongwei Liang, Guangfu Hu, Qinchao Wu, Guiwei Zou, Xiangzhong Luo. Genetic diversity in fourProcambarus clarkii populations in the lower reaches of the Yangtze River[J]. Biodiv Sci, 2009, 17(5): 518-523.

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URL: https://www.biodiversity-science.net/EN/10.3724/SP.J.1003.2009.09017

https://www.biodiversity-science.net/EN/Y2009/V17/I5/518

Figures/Tables 5

References 12

[1]	Barbaresi S, Fani R, Gherardi F, Mengoni A, Souty-Grosset C (2003) Genetic variability in European populations of an invasive American crayfish: preliminary results. Biological Invasions, 5,269-274.
[2]	Barbaresi S, Fani R, Gherardi F, Mengoni A, Souty-Grosset C (2007) Genetics and invasion biology in fresh waters: a pilot study of Procambarus clarkii in Europe. Biological Invaders in Inland Waters: Profiles, Distribution, and Threats, 4,381-400.
[3]	Belfiore NM, May B (2000) Variable microsatellite loci in red swamp crayfish, Procambarus clarkii, and their characterization in other crayfish taxa. Molecular Ecology, 9,2231-2233. URL PMID
[4]	Botstein D, White RL, Skolnick M, Davis RW (1980) Construction of a genetic linkage map in man using restriction fragment length polymorphisms. The American Journal of Human Genetics, 32,314-331. URL PMID
[5]	Huner JV (1988) Procambarus in North America and elsewhere. In: Freshwater Crayfish: Biology, Management and Exploitation (eds Holdich DM, Lowery RS), pp.239-261. Croom Helm,London & Sydney and Timber Press, Portland.
[6]	Lu CY (鲁翠云), Jin WK (金万昆), Sun XW (孙效文), Li DY (李大宇), Zhu XD (朱晓东), Ma HT (马海涛), Yu DM (于东梅), Yang JX (杨建新) (2008) Effects of sample size on various genetic structure parameter in cultured population genetic study. Journal of Fisheries of China (水产学报), 32,674-683. (in Chinese with English abstract)
[7]	Rozas J, Hernandez M, Cabrera VM, Prevosti A (1990) Colonization of America by Drosophila subobscura: effect of the founder event on the mitochondrial DNA polymorphism. Molecular Biology and Evolution, 7,103-109. DOI URL PMID
[8]	Sambrook J, Fritsch EF, Maniatis T (1989) Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor Laboratory Press, New York.
[9]	Shen JR (沈嘉瑞) (1976) Shrimps and crabs in China (中国的虾蟹). Science Press, Beijing. (in Chinese)
[10]	Stepine CA, Taylor CD, Dabrowska KA (2002) Genetic variability and phylogeographical patterns of a nonindigenous species invasion: a comparison of exotic vs. native zebra and quagga mussel populations. Journal of Evolutionary Biology, 15,314-328.
[11]	Wang WM (王卫民) (1999) The exploitation and utilization of red swamp crayfish in China. Acta Hydrobiologica Sinica (水生生物学报), 23,375-381. (in Chinese with English abstract)
[12]	Yue GH, Wang GL, Zhu BQ, Wang CM, Zhu ZY, Lo LC (2008) Discovery of four natural clones in a crayfish species Procambarus clarkii. International Journal of Biological Sciences, 4,279-282. DOI URL PMID

座位 Locus	引物序列(5′→3′) Primer sequence(5′→3′)	退火温度 Annealing temperature (℃)	重复类型 SSR motif	片段大小 Allele size (bp)	等位基因No. of alleles	登录号 GenBank accession no.
PclG02	F:CTCCCCATGCACTCTGGCTCTGT R:TGGCGAATTTTGCCTGTTTCTGTC	66	(GATA)₃GAGAA(GATA)₅	216-224	3	AF290919
PclG03	F:CTCTCCACCAGTCATTTCTT R:AAGCTTACAATAAATATAGATAGAC	52	(TCTA)₂₀	216-420	4	AF290920
PclG04	F:TATATCAGTCAATCTGTCCAG R:TCAGTAAGTAGATTGATAGAAGG	54	(TCTA)₃…(TCTA)₂…(TCTA)₂₉… (TCTA)₂	170-290	4	AF290921
PclG07	F:CCTCCCACCAGGGTTATCTATTCA R:GTGGGTGTGGCGCTCTTGTT	63	(TCTA)₈	100-160	4	AF290922
PclG08	F:ACGATAAATGGATAGATGGATGAA R:CCGGGTCTGTCTGTCTGTCA	62	(GATA)₁₆	148-220	5	AF290923
PclG09	F:TATGCACCTTTACCTGAAT R:TGTTGGTGTGGTCATCA	60	(TCTA)₁₄	80-160	4	AF290924
PclG10	F:TGCTCACGCAAACTTGTATTCAGT R:CAATGGTCCTTGATTTGGTGTTCT	54	(TAGA)₂TA(TAGA)₁₆	90-176	3	AF290925
PclG13	F:CTCTCCTGGCGCTGTTATTTAGC R:TGAAGAGGCAGAGTGAGGATTCTC	62	(TCTA)₁₂	130-150	2	AF290926
PclG15	F:GGCGTGACGCCAACGTGTCTT R:GGCTGGCCACTTTGTTAGCCTGAG	70	(TATC)₂TGTC(TATC)₁₇TATT(TATC)₃	150-185	3	AF290927
PclG16	F:CTCGGAATGTCCACCTGAGA R:TCATTATGGATTTTGTCAATCTAT	54	(TCTA)₁₈TCTC(TATC)₃	80-160	4	AF290928
PclG17	F:GTCGGGAACCTATTTACAGTGTAT R:AAGAGCGAAGAAAGAGATAAAGAT	57	(TCTA)₁₄	156-190	4	AF290929
PclG27	F:AATCTTAAGATCATGAAAAAGGTA R:TTTAAGGAACGTATAAGAAAAGAC	57	(TATC)₄CATC(TATC)₈	80-150	6	AF290932
PclG28	F:CTCGGCGAGTTTACTGAAAT R:AGAAGAAAGGGATATAAGGTAAAG	60	(GATA)₂₂(GA)₅	210-270	3	AF290933
PclG29	F:GAAAGTCATGGGTGTAGGTGTAAC R:TTTTTGGGCTATGTGACGAG	65	(TATC)₉	95-165	3	AF290934
PclG33	F:TTCGAGGCGTTGCTGATTGTAAGT R:CAAGGAAGCGTATAGCCGGAGTCT	68	(GT)₂₁	120-180	5	AF290936
PclG37	F:TAAATAAGTGGCGTGTAAGACGAG R:TAACTAAGCCAGGGTGGTCTCCAG	66	(CA)₄CG(CA)₁₅CG (CA)₂₃	80-180	7	AF290939
PclG48	F:CTGTTGGTGATTTCCGTCAATTTT R:AGATTCAACGCTGTGTTCCTGATC	66	(CA)₁₂	146-190	2	AF290941

座位 Locus	引物序列(5′→3′) Primer sequence(5′→3′)	退火温度 Annealing temperature (℃)	重复类型 SSR motif	片段大小 Allele size (bp)	等位基因No. of alleles	登录号 GenBank accession no.
PclG02	F:CTCCCCATGCACTCTGGCTCTGT R:TGGCGAATTTTGCCTGTTTCTGTC	66	(GATA)₃GAGAA(GATA)₅	216-224	3	AF290919
PclG03	F:CTCTCCACCAGTCATTTCTT R:AAGCTTACAATAAATATAGATAGAC	52	(TCTA)₂₀	216-420	4	AF290920
PclG04	F:TATATCAGTCAATCTGTCCAG R:TCAGTAAGTAGATTGATAGAAGG	54	(TCTA)₃…(TCTA)₂…(TCTA)₂₉… (TCTA)₂	170-290	4	AF290921
PclG07	F:CCTCCCACCAGGGTTATCTATTCA R:GTGGGTGTGGCGCTCTTGTT	63	(TCTA)₈	100-160	4	AF290922
PclG08	F:ACGATAAATGGATAGATGGATGAA R:CCGGGTCTGTCTGTCTGTCA	62	(GATA)₁₆	148-220	5	AF290923
PclG09	F:TATGCACCTTTACCTGAAT R:TGTTGGTGTGGTCATCA	60	(TCTA)₁₄	80-160	4	AF290924
PclG10	F:TGCTCACGCAAACTTGTATTCAGT R:CAATGGTCCTTGATTTGGTGTTCT	54	(TAGA)₂TA(TAGA)₁₆	90-176	3	AF290925
PclG13	F:CTCTCCTGGCGCTGTTATTTAGC R:TGAAGAGGCAGAGTGAGGATTCTC	62	(TCTA)₁₂	130-150	2	AF290926
PclG15	F:GGCGTGACGCCAACGTGTCTT R:GGCTGGCCACTTTGTTAGCCTGAG	70	(TATC)₂TGTC(TATC)₁₇TATT(TATC)₃	150-185	3	AF290927
PclG16	F:CTCGGAATGTCCACCTGAGA R:TCATTATGGATTTTGTCAATCTAT	54	(TCTA)₁₈TCTC(TATC)₃	80-160	4	AF290928
PclG17	F:GTCGGGAACCTATTTACAGTGTAT R:AAGAGCGAAGAAAGAGATAAAGAT	57	(TCTA)₁₄	156-190	4	AF290929
PclG27	F:AATCTTAAGATCATGAAAAAGGTA R:TTTAAGGAACGTATAAGAAAAGAC	57	(TATC)₄CATC(TATC)₈	80-150	6	AF290932
PclG28	F:CTCGGCGAGTTTACTGAAAT R:AGAAGAAAGGGATATAAGGTAAAG	60	(GATA)₂₂(GA)₅	210-270	3	AF290933
PclG29	F:GAAAGTCATGGGTGTAGGTGTAAC R:TTTTTGGGCTATGTGACGAG	65	(TATC)₉	95-165	3	AF290934
PclG33	F:TTCGAGGCGTTGCTGATTGTAAGT R:CAAGGAAGCGTATAGCCGGAGTCT	68	(GT)₂₁	120-180	5	AF290936
PclG37	F:TAAATAAGTGGCGTGTAAGACGAG R:TAACTAAGCCAGGGTGGTCTCCAG	66	(CA)₄CG(CA)₁₅CG (CA)₂₃	80-180	7	AF290939
PclG48	F:CTGTTGGTGATTTCCGTCAATTTT R:AGATTCAACGCTGTGTTCCTGATC	66	(CA)₁₂	146-190	2	AF290941

座位 Locus	南京群体 Nanjing population				盱眙群体 Xuyi population					合肥群体 Hefei population					南昌群体 Nanchang population
座位 Locus	PIC	观测杂合度 H_o	期望杂合度 H_e	平衡偏离指数 D		PIC	观测杂合度 H_o	期望杂合度 H_e	平衡偏离指数 D		PIC	观测杂合度 H_o	期望杂合度 H_e	平衡偏离指数 D		PIC	观测杂合度 H_o	期望杂合度 H_e	平衡偏离指数 D
PclG02	0.4446	0.4894	0.5067	-0.0341	0.4296		0.4783	0.5380	-0.1110	0.1693		0.1250	0.1886	-0.3372	0.5226		0.6250	0.6116	0.0219
PclG03	0.5127	0.4103	0.6051	-0.3219	0.5677		0.6222	0.6492	-0.0416	0.3705		0.5111	0.4966	0.0292	0.4087		0.5000	0.5290	-0.0548
PclG04	0.6663	0.3542	0.7265	-0.5125	0.3018		0.2292	0.3537	-0.3520	0.4868		0.5208	0.5524	-0.0572	0.3680		0.4583	0.4912	-0.0670
PclG07	0.5020	0.4894	0.5546	-0.1176	0.4536		0.3750	0.5570	-0.3268	0.4346		0.3750	0.4974	-0.2461	0.4039		0.4583	0.5068	-0.0957
PclG08	0.6980	0.3409	0.7531	-0.5473	0.5592		0.4583	0.6397	-0.2836	0.4093		0.5000	0.5267	-0.0507	0.4101		0.3542	0.4721	-0.2497
PclG09	0.6377	0.2500	0.7017	-0.6437	0.3775		0.1707	0.4279	-0.6011	0.4356		0.5814	0.5453	0.0662	0.5279		0.1591	0.6142	-0.7410
PclG10	0.4331	0.3864	0.5421	-0.2872	0.3664		0.0811	0.4498	-0.8197	0.0000		0.0000	0.0000	0.0000	0.2454		0.2045	0.2652	-0.2289
PclG13	0.3660	0.3542	0.4875	-0.2734	0.3225		0.3958	0.4086	-0.0313	0.0000		0.0000	0.0000	0.0000	0.3444		0.3617	0.4468	-0.1905
PclG15	0.6289	0.2292	0.2623	-0.1262	0.3263		0.4130	0.3641	0.1343	0.5916		0.7234	0.6728	0.0752	0.0976		0.1042	0.1011	0.0307
PclG16	0.6800	0.6170	0.7369	-0.1627	0.5934		0.5778	0.6504	-0.1116	0.4485		0.4643	0.5292	-0.1226	0.6629		0.6250	0.7230	-0.1356
PclG17	0.5867	0.4583	0.6607	-0.3063	0.4836		0.3750	0.5401	-0.3057	0.0000		0.0000	0.0000	0.0000	0.5642		0.6458	0.6465	-0.0011
PclG27	0.6902	0.5909	0.7450	-0.2069	0.6812		0.7391	0.7360	0.0042	0.3045		0.4255	0.3672	0.1588	0.6853		0.6809	0.7380	-0.0774
PclG28	0.5517	0.2045	0.6335	-0.6772	0.4092		0.1087	0.4909	-0.7786	0.0000		0.0000	0.0000	0.0000	0.3733		0.3750	0.5018	-0.2527
PclG29	0.4904	0.3830	0.5726	-0.3311	0.4366		0.6522	0.5162	0.2635	0.5558		0.5870	0.6362	-0.0773	0.3884		0.3043	0.5115	-0.4051
PclG33	0.5800	1.0000	0.6536	0.5300	0.6700		1.0000	0.7550	0.3245	0.5862		1.0000	0.6577	0.5205	0.7290		1.0000	0.7746	0.2910
PclG37	0.7231	1.0000	0.7703	0.2982	0.6837		1.0000	0.7389	0.3534	0.6470		1.0000	0.7076	0.4132	0.5673		1.0000	0.6436	0.5538
PclG48	0.3686	0.4318	0.4929	-0.1240	0.2490		0.3125	0.2945	0.0611	0.3533		0.5333	0.4634	0.1508	0.3629		0.3478	0.4816	-0.2778

座位 Locus	南京群体 Nanjing population				盱眙群体 Xuyi population					合肥群体 Hefei population					南昌群体 Nanchang population
座位 Locus	PIC	观测杂合度 H_o	期望杂合度 H_e	平衡偏离指数 D		PIC	观测杂合度 H_o	期望杂合度 H_e	平衡偏离指数 D		PIC	观测杂合度 H_o	期望杂合度 H_e	平衡偏离指数 D		PIC	观测杂合度 H_o	期望杂合度 H_e	平衡偏离指数 D
PclG02	0.4446	0.4894	0.5067	-0.0341	0.4296		0.4783	0.5380	-0.1110	0.1693		0.1250	0.1886	-0.3372	0.5226		0.6250	0.6116	0.0219
PclG03	0.5127	0.4103	0.6051	-0.3219	0.5677		0.6222	0.6492	-0.0416	0.3705		0.5111	0.4966	0.0292	0.4087		0.5000	0.5290	-0.0548
PclG04	0.6663	0.3542	0.7265	-0.5125	0.3018		0.2292	0.3537	-0.3520	0.4868		0.5208	0.5524	-0.0572	0.3680		0.4583	0.4912	-0.0670
PclG07	0.5020	0.4894	0.5546	-0.1176	0.4536		0.3750	0.5570	-0.3268	0.4346		0.3750	0.4974	-0.2461	0.4039		0.4583	0.5068	-0.0957
PclG08	0.6980	0.3409	0.7531	-0.5473	0.5592		0.4583	0.6397	-0.2836	0.4093		0.5000	0.5267	-0.0507	0.4101		0.3542	0.4721	-0.2497
PclG09	0.6377	0.2500	0.7017	-0.6437	0.3775		0.1707	0.4279	-0.6011	0.4356		0.5814	0.5453	0.0662	0.5279		0.1591	0.6142	-0.7410
PclG10	0.4331	0.3864	0.5421	-0.2872	0.3664		0.0811	0.4498	-0.8197	0.0000		0.0000	0.0000	0.0000	0.2454		0.2045	0.2652	-0.2289
PclG13	0.3660	0.3542	0.4875	-0.2734	0.3225		0.3958	0.4086	-0.0313	0.0000		0.0000	0.0000	0.0000	0.3444		0.3617	0.4468	-0.1905
PclG15	0.6289	0.2292	0.2623	-0.1262	0.3263		0.4130	0.3641	0.1343	0.5916		0.7234	0.6728	0.0752	0.0976		0.1042	0.1011	0.0307
PclG16	0.6800	0.6170	0.7369	-0.1627	0.5934		0.5778	0.6504	-0.1116	0.4485		0.4643	0.5292	-0.1226	0.6629		0.6250	0.7230	-0.1356
PclG17	0.5867	0.4583	0.6607	-0.3063	0.4836		0.3750	0.5401	-0.3057	0.0000		0.0000	0.0000	0.0000	0.5642		0.6458	0.6465	-0.0011
PclG27	0.6902	0.5909	0.7450	-0.2069	0.6812		0.7391	0.7360	0.0042	0.3045		0.4255	0.3672	0.1588	0.6853		0.6809	0.7380	-0.0774
PclG28	0.5517	0.2045	0.6335	-0.6772	0.4092		0.1087	0.4909	-0.7786	0.0000		0.0000	0.0000	0.0000	0.3733		0.3750	0.5018	-0.2527
PclG29	0.4904	0.3830	0.5726	-0.3311	0.4366		0.6522	0.5162	0.2635	0.5558		0.5870	0.6362	-0.0773	0.3884		0.3043	0.5115	-0.4051
PclG33	0.5800	1.0000	0.6536	0.5300	0.6700		1.0000	0.7550	0.3245	0.5862		1.0000	0.6577	0.5205	0.7290		1.0000	0.7746	0.2910
PclG37	0.7231	1.0000	0.7703	0.2982	0.6837		1.0000	0.7389	0.3534	0.6470		1.0000	0.7076	0.4132	0.5673		1.0000	0.6436	0.5538
PclG48	0.3686	0.4318	0.4929	-0.1240	0.2490		0.3125	0.2945	0.0611	0.3533		0.5333	0.4634	0.1508	0.3629		0.3478	0.4816	-0.2778

群体 Population	代号 Code	平均等位基因数 Mean N_a	平均有效等位基因数 Mean N_e	平均多态信息含量 Mean PIC	平均观测杂合度 Mean H_o	平均期望杂合度 Mean H_e
南京群体 Nanjing population	NJ	3.7647	2.7778	0.5624	0.4700	0.6121
盱眙群体 Xuyi population	XY	3.4706	2.3455	0.4654	0.4699	0.5359
合肥群体 Hefei population	HF	2.5294	1.9415	0.3408	0.4322	0.4024
南昌群体 Nanchang population	NC	3.2941	2.3656	0.4507	0.4826	0.5329
平均 Mean		3.2647	3.3576	0.4548	0.4637	0.5208