生物多样性 ›› 2008, Vol. 16 ›› Issue (6): 593-600. DOI: 10.3724/SP.J.1003.2008.08121
所属专题: 土壤生物与土壤健康
收稿日期:
2008-05-27
接受日期:
2008-10-27
出版日期:
2008-11-20
发布日期:
2008-11-20
通讯作者:
何琳燕
基金资助:
Fei Zhao, Xiafang Sheng, Zhi Huang, Linyan He()
Received:
2008-05-27
Accepted:
2008-10-27
Online:
2008-11-20
Published:
2008-11-20
Contact:
Linyan He
About author:
* E-mail: helyan0794@njau.edu.cn摘要:
不同土壤类型钾矿物分解细菌资源调查和高效稳定释钾、促生细菌的筛选鉴定有助于丰富微生物资源库,发掘和利用钾矿物分解细菌以及探究矿物生物风化机理等。作者采用以钾长石为唯一钾源的选择性细菌培养基, 从山东地区不同土壤和不同植物根际土壤中分离纯化了23株生长势良好的钾矿物分解细菌, 通过测定细菌代谢产物IAA和铁载体,研究其产生促生物质的能力, 通过摇瓶试验筛选高效释钾菌株, 采用16S rDNA限制性酶切多态性分析(amplified rDNA restriction analysis, ARDRA)方法研究了钾矿物分解细菌的遗传多样性, 根据16S rDNA同源性对高效释钾菌株进行了鉴定。结果表明, 供试菌株均产吲哚乙酸或其衍生物, 43.5%的分离菌株产极高量铁载体。ARDRA结果表明供试菌株在60%相似性水平上可分为11个基因型, 同一类型土壤上不同作物根际或不同类型土壤上同一作物根际的钾矿物分解细菌存在明显的遗传差异。摇瓶试验结果表明供试菌株中具有较显著释钾能力的菌株占17%, 39%的供试菌株无释钾能力。筛选到2株高效释钾菌株AFM2、AC2, 分别使溶液中钾含量增加了29.8%和25.4%。16S rDNA同源性分析表明菌株AC2、AHZ1与Bacillus mucilaginosus聚为一群, 该群与包含菌株AZH4的Paenibacillussp.中的种聚为一大发育分支, 该分支在细菌分类地位上隶属于Firmicutes; 菌株AFM2与Rhizobium sp. 和Agrobacterium tumefaciens聚为另一大发育分支, 该分支在细菌分类地位上隶属于Alphaproteobacteria。
赵飞, 盛下放, 黄智, 何琳燕 (2008) 山东地区钾矿物分解细菌的分离及生物学特性. 生物多样性, 16, 593-600. DOI: 10.3724/SP.J.1003.2008.08121.
Fei Zhao, Xiafang Sheng, Zhi Huang, Linyan He (2008) Isolation of mineral potassium-solubilizing bacterial strains from agricultural soils in Shandong Province. Biodiversity Science, 16, 593-600. DOI: 10.3724/SP.J.1003.2008.08121.
菌株 Strains | 采样地点 Sampling sites | 土壤类型 Soil type | 植物种类 Plants | 代谢产物 Metabolism products | |
---|---|---|---|---|---|
吲哚乙酸 IAA | 铁载体 Sidersphore | ||||
AC2 | 德州 Dezhou City | 潮土 Fluvo-aquic soil | 无 Bulk | + + | + + |
ACY2 | 德州 Dezhou City | 潮土 Fluvo-aquic soil | 玉米 Maize | + + | + + |
ACM2 | 德州 Dezhou City | 潮土 Fluvo-aquic soil | 棉花 Cotton | + | + |
ACG2 | 德州 Dezhou City | 潮土 Fluvo-aquic soil | 高粱 Sorghum | + + | – |
ACD2 | 德州 Dezhou City | 潮土 Fluvo-aquic soil | 大豆 Soybean | + | + + |
ACZ2 | 德州 Dezhou City | 潮土 Fluvo-aquic soil | 杂草 Weeds | + + | + + |
ACY3 | 曹县 Caoxian County | 潮土 Fluvo-aquic soil | 玉米 Maize | + + | – |
ACZ3 | 曹县 Caoxian County | 潮土 Fluvo-aquic soil | 杂草 Weeds | + | – |
AZZ4 | 临沭 Linshu County | 棕壤 Brown earth | 杂草 Weeds | + + | + + |
AZH4 | 临沭 Linshu County | 棕壤 Brown earth | 红薯 Sweet potato | + | + |
AZZ5 | 威海 Weihai City | 棕壤 Brown earth | 杂草 Weeds | + + | + + |
AHY1 | 广饶 Guangrao County | 褐土 Cinnamon soil | 玉米 Maize | + + | – |
AHZ1 | 广饶 Guangrao County | 褐土 Cinnamon soil | 杂草 Weeds | + | + + |
ASY1 | 广饶 Guangrao County | 砂姜黑土 Lime concretion black soil | 玉米 Maize | + | + + |
ASG1 | 广饶 Guangrao County | 砂姜黑土 Lime concretion black soil | 高粱 Sorghum | + | + + |
ASZ1 | 广饶 Guangrao County | 砂姜黑土 Lime concretion black soil | 杂草 Weeds | + + | – |
AZY2 | 德州 Dezhou City | 草甸盐土 Meadow solonchak | 玉米 Maize | ++ | + |
AZZ2 | 德州 Dezhou City | 草甸盐土 Meadow solonchak | 杂草 Weeds | + | + |
AF2 | 德州 Dezhou City | 风沙土 Aeolian soil | 无 Bulk | + | – |
AFM2 | 德州 Dezhou City | 风沙土 Aeolian soil | 棉花 Cotton | + + | – |
AFY2 | 德州 Dezhou City | 风沙土 Aeolian soil | 玉米 Maize | + | + |
AY3 | 曹县 Caoxian County | 碱土 Solontz | 无 Bulk | + | + + |
AYM3 | 曹县 Caoxian County | 碱土 Solontz | 棉花 Cotton | + | + |
表1 菌株的来源及代谢产物吲哚乙酸(IAA)和铁载体的分泌特征
Table 1 Indoleacetic acid (IAA) and sidersphore production of isolates obtained from the rhizosphere of various plants in different soils types, Shandong Province
菌株 Strains | 采样地点 Sampling sites | 土壤类型 Soil type | 植物种类 Plants | 代谢产物 Metabolism products | |
---|---|---|---|---|---|
吲哚乙酸 IAA | 铁载体 Sidersphore | ||||
AC2 | 德州 Dezhou City | 潮土 Fluvo-aquic soil | 无 Bulk | + + | + + |
ACY2 | 德州 Dezhou City | 潮土 Fluvo-aquic soil | 玉米 Maize | + + | + + |
ACM2 | 德州 Dezhou City | 潮土 Fluvo-aquic soil | 棉花 Cotton | + | + |
ACG2 | 德州 Dezhou City | 潮土 Fluvo-aquic soil | 高粱 Sorghum | + + | – |
ACD2 | 德州 Dezhou City | 潮土 Fluvo-aquic soil | 大豆 Soybean | + | + + |
ACZ2 | 德州 Dezhou City | 潮土 Fluvo-aquic soil | 杂草 Weeds | + + | + + |
ACY3 | 曹县 Caoxian County | 潮土 Fluvo-aquic soil | 玉米 Maize | + + | – |
ACZ3 | 曹县 Caoxian County | 潮土 Fluvo-aquic soil | 杂草 Weeds | + | – |
AZZ4 | 临沭 Linshu County | 棕壤 Brown earth | 杂草 Weeds | + + | + + |
AZH4 | 临沭 Linshu County | 棕壤 Brown earth | 红薯 Sweet potato | + | + |
AZZ5 | 威海 Weihai City | 棕壤 Brown earth | 杂草 Weeds | + + | + + |
AHY1 | 广饶 Guangrao County | 褐土 Cinnamon soil | 玉米 Maize | + + | – |
AHZ1 | 广饶 Guangrao County | 褐土 Cinnamon soil | 杂草 Weeds | + | + + |
ASY1 | 广饶 Guangrao County | 砂姜黑土 Lime concretion black soil | 玉米 Maize | + | + + |
ASG1 | 广饶 Guangrao County | 砂姜黑土 Lime concretion black soil | 高粱 Sorghum | + | + + |
ASZ1 | 广饶 Guangrao County | 砂姜黑土 Lime concretion black soil | 杂草 Weeds | + + | – |
AZY2 | 德州 Dezhou City | 草甸盐土 Meadow solonchak | 玉米 Maize | ++ | + |
AZZ2 | 德州 Dezhou City | 草甸盐土 Meadow solonchak | 杂草 Weeds | + | + |
AF2 | 德州 Dezhou City | 风沙土 Aeolian soil | 无 Bulk | + | – |
AFM2 | 德州 Dezhou City | 风沙土 Aeolian soil | 棉花 Cotton | + + | – |
AFY2 | 德州 Dezhou City | 风沙土 Aeolian soil | 玉米 Maize | + | + |
AY3 | 曹县 Caoxian County | 碱土 Solontz | 无 Bulk | + | + + |
AYM3 | 曹县 Caoxian County | 碱土 Solontz | 棉花 Cotton | + | + |
图1 液体培养条件下不同菌株分解钾长石释钾效能比较,接灭活菌为对照。
Fig. 1 Amount of potassium (K) released by bacterial strains after four days inoculation in liquid cultures. Controls were inoculated by autoclaved inocula.
图2 根据16S rDNA限制性酶切片段分析得到的供试菌株聚类树状图
Fig. 2 A UPGMA dendrogram of 23 potassium-releasing bacterial strains based on amplified rDNA restriction analysis (ARDRA) patterns from Hae Ⅲ and MspⅠdigestion.
图3 基于2个高效释钾菌株AC2、AFM2和2个中等释钾效能菌株AHZ1、AZH4以及亲缘关系相近的菌株(分别属于Bacillus, Paebacillus, Rhizobium和Agrobacterium属)的16S rDNA序列构建的系统发育树,标尺代表进化距离。
Fig. 3 The phylogenetic tree based on the 16S rDNA sequences of four potassium-bearing mineral-dissolved bacteria strains and those of related species in the genusBacillus, Paebacillus, Rhizobium, and Agrobacterium. Scale bar indicates evolutionary distance.
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