海南长臂猿线粒体D-loop区序列分析及种群复壮

doi:10.3724/SP.J.1003.2010.523

生物多样性 ›› 2010, Vol. 18 ›› Issue (5): 523-527. DOI: 10.3724/SP.J.1003.2010.523 cstr: 32101.14.SP.J.1003.2010.523

• 研究简报 • 上一篇

海南长臂猿线粒体D-loop区序列分析及种群复壮

李志刚¹, 魏辅文², 周江¹^,²^,^*()

1 贵州师范大学生命科学学院, 贵阳 550001
2 中国科学院动物研究所, 北京 100101

收稿日期:2010-03-02 接受日期:2010-08-22 出版日期:2010-09-20 发布日期:2010-09-20
通讯作者: 周江
作者简介:* E-mail: prattiip2006@vip.sohu.com
基金资助:
香港嘉道理农场暨植物园奖学金项目

Mitochondrial DNA D-loop sequence analysis and population rejuvena- tion of Hainan gibbons (Nomascus hainanus)

Zhigang Li¹, Fuwen Wei², Jiang Zhou¹^,²^,^*()

1 School of Life Sciences, Guizhou Normal University, Guiyang 550001
2 Institute of Zoology, the Chinese Academy of Sciences, Beijing 100101

Received:2010-03-02 Accepted:2010-08-22 Online:2010-09-20 Published:2010-09-20
Contact: Jiang Zhou

摘要/Abstract

摘要：

海南长臂猿(Nomascus hainanus)是世界上最濒危的灵长类动物之一, 但目前有关海南长臂猿的种群遗传学方面的信息以及种群复壮所面临的困难未见报道。为更好地保护该极危物种, 作者以粪便为研究材料, 首次在分子生物学水平上测定了海南长臂猿1个群体(B群)共6个个体的线粒体D-loop区基因序列。结果显示: 202 bp的D-loop区基因共检测到5个变异位点, 4个单倍型, 单倍型多样性(h)为0.6000, 核苷酸多样性(π)为0.00829, 表明海南长臂猿B群的遗传多样性较低; 与此同时, 海南长臂猿还面临着种群数量过小, 性比失衡, 以及栖息地质量低下等严峻的问题。

关键词: Nomascus hainanus, 粪便DNA, 线粒体D-loop, 遗传多样性

Abstract

The Hainan gibbon (Nomascus hainanus) is one among the most endangered primates globally according to Critically Endangered List of IUCN. However, there has been no report on its genetic information and the difficulties of its population restoration. This study represents the first time survey of genetic diversity using mitochondrial D-loop sequences from faecal sampling of six individuals within one group. Five variable sites were detected in the 202 bp D-loop sequences and 4 haplotypes were defined. The haplotype diversity (h) and nucleotide diversity (π) were 0.6000 and 0.00829, respectively, meaning the genetic diversity was low. Furthermore, this critically endangered species also face other threatening factors, such as small population size, biased sex ratio and low habitat quality.

Key words: Nomascus hainanus, faecal DNA, mitochondrial DNA D-loop, genetic diversity

李志刚, 魏辅文, 周江 (2010) 海南长臂猿线粒体D-loop区序列分析及种群复壮. 生物多样性, 18, 523-527. DOI: 10.3724/SP.J.1003.2010.523.

Zhigang Li, Fuwen Wei, Jiang Zhou (2010) Mitochondrial DNA D-loop sequence analysis and population rejuvena- tion of Hainan gibbons (Nomascus hainanus). Biodiversity Science, 18, 523-527. DOI: 10.3724/SP.J.1003.2010.523.

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链接本文: https://www.biodiversity-science.net/CN/10.3724/SP.J.1003.2010.523

https://www.biodiversity-science.net/CN/Y2010/V18/I5/523

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参考文献 19

[1]	Chivers DJ (1974) The siamang in Malaysia: a field study of a primate in a tropical rain forest. Contributions to Primatology, 4, 1-335.
[2]	Cowlishaw G, Dunbar R (2000) Primate Conservation Biology. The University of Chicago Press, Chicago.
[3]	Frankham R (1998) Inbreeding and extinction: island populations. Conservation Biology, 12, 665-675.
[4]	Frankham R, Ballou JD, Briscoe DA (2002) Introduction to Conservation Genetics. Cambridge University Press, Cambridge.
[5]	He L (何丽), Zhang YG (张于光), Li DQ (李迪强), Li DQ (李大全) (2010) Analysis on mitochondrial DNA D-loop sequences genetic polymorphism of Rhinopithecus roxellana. Chinese Journal of Zoology (动物学杂志), 45(1), 70-76. (in Chinese with English abstract)
[6]	Hayaishi SH, Kawamoto YH (2006) Low genetic diversity and biased distribution of mitochondrial DNA haplotypes in the Japanese macaque (Macaca fuscata yakui) on Yakushima Island. Primates, 47, 158-164.
[7]	Irwin DW, Kcher TD, Wilson AC (1991) Evolution of cytochrome b gene of mammals. Journal of Molecular Evolution, 32, 128-144.
[8]	Jeanmougin F, Thompson JD, Gouy M, Higgins DG, Gibson TJ (1998) Multiple sequence alignment with Clustal X. Trends in Biochemical Sciences, 23, 403-405. URL PMID
[9]	Kressirer P (1993) Eine Molekulare Phylogenie der Gibbons (Hylobatidae). Diplomarbeit der Fakultat fur Biologie der Ludwig-Maximilians-Universitat, Munchen.
[10]	Kumar S, Tamura K, Jakobusen IB, Nei M (2001) MEGA2: molecular evolutionary genetics analysis software. Bioinformatics, 17, 1244-1245. DOI URL PMID
[11]	Liu ZJ, Ren BP, Wei FW, Long YC, Hao YL, Li M (2007) Phylogeography and population structure of the Yunnan snub-nosed monkey (Rhinopithecus bieti) inferred from mitochondrial control region DNA sequence analysis. Molecular Ecology, 16, 3334-3349. DOI URL PMID
[12]	Liu ZH, Jiang HS (1989) Population structure of Hylobates concolor in Bawangling Nature Reserve, Hainan, China. American Journal of Primatology, 19, 247-254. DOI URL PMID
[13]	Monda K, Simmons RE, Kressirer P, Su B, Woodruff DS (2007) Mitochondrial DNA hypervariable region-1 sequence variation and phylogeny of the concolor gibbons, Nomascus. American Journal of Primatology, 69, 1285-1306.
[14]	Rozas J, Rozas R (1999) DnaSP version 3: an integrated program for molecular population genetics and molecular evolution analysis. Bioinformatics, 15, 174-175. URL PMID
[15]	Soulé ME, Orians GH, Boersma PD (2001) Conservation Biology: Research Priorities for the Next Decade. Society for Conservation Biology, Island Press, Washington.
[16]	Young AG, Clarke GM (2000) Genetics Demography and Variability of Fragment Population. Cambridge University Press, London.
[17]	Zhou J, Wei FW, Li M, Zhang JF, Wang DL, Pan RL (2005) Hainan black-crested gibbon is headed for extinction. International Journal of Primatology, 26, 453-465.
[18]	Zhang BW, Li M, Ma LC, Wei FW (2006) A widely applicable protocol for DNA isolation from fecal samples. Biochemical Genetics, 44, 503-512.
[19]	Zhou J, Wei FW, Li M, Pui Lok CB, Wang DL (2008) Reproductive characters and mating behaviour of wild Nomascus hainanus. International Journal of Primatology, 29, 1037-1046.

Indiv- idual codes	变异位点 Variable sites
Indiv- idual codes	1-10	11-20	21-30	31-40	41-50	51-60	61-70	71-80	81-90
1	TAGAACATCC	CCTCCCCATT	TCAACATTCC	AAACCTACCC	AACATGCGTA	TCAACCAACC	AAGATAGTCC	ATCTCGGACA	TGGCACATTA
2	- - - G - - - - - -	- - - TT - - - - -	- - - - - - - C - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -
3	- - - G - - - - - -	- - - TT - - - - -	- - - - - - - C - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -	- - A- - - - - - -
4	- - - G - - - - - -	- - - TT - .- - -	- - - - - - - C - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -
5	- - - G - - - - - -	- - - TT - - - - -	- - - - - - - C - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -
6	- - - G - - - - - -	- - - TT - - - - -	- - - - - - - C - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -

Indiv- idual codes	变异位点 Variable sites
Indiv- idual codes	1-10	11-20	21-30	31-40	41-50	51-60	61-70	71-80	81-90
1	TAGAACATCC	CCTCCCCATT	TCAACATTCC	AAACCTACCC	AACATGCGTA	TCAACCAACC	AAGATAGTCC	ATCTCGGACA	TGGCACATTA
2	- - - G - - - - - -	- - - TT - - - - -	- - - - - - - C - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -
3	- - - G - - - - - -	- - - TT - - - - -	- - - - - - - C - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -	- - A- - - - - - -
4	- - - G - - - - - -	- - - TT - .- - -	- - - - - - - C - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -
5	- - - G - - - - - -	- - - TT - - - - -	- - - - - - - C - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -
6	- - - G - - - - - -	- - - TT - - - - -	- - - - - - - C - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -	- - - - - - - - - -

海南长臂猿线粒体D-loop区序列分析及种群复壮

Mitochondrial DNA D-loop sequence analysis and population rejuvena- tion of Hainan gibbons (Nomascus hainanus)

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