菜粉蝶对两种迁地保护的兰科植物传粉和繁殖成功的作用

doi:10.3724/SP.J.1003.2010.011

生物多样性 ›› 2010, Vol. 18 ›› Issue (1): 11-18. DOI: 10.3724/SP.J.1003.2010.011

所属专题：传粉生物学；昆虫多样性与生态功能；生物入侵

菜粉蝶对两种迁地保护的兰科植物传粉和繁殖成功的作用

张洪芳¹^,³, 李利强², 刘仲健², 罗毅波¹^,²^,^*()

1 中国科学院植物研究所系统与进化植物学国家重点实验室, 北京 100093
2 全国兰科植物种质资源保护中心, 广东深圳 518114
3 中国科学院研究生院, 北京 100049

收稿日期:2009-06-15 接受日期:2009-12-16 出版日期:2010-01-20 发布日期:2010-01-20
通讯作者: 罗毅波
作者简介:* E-mail: luoyb@ibcas.ac.cn
基金资助:
深圳市科学技术计划项目(SY200704300157A)

The butterfly Pieris rapae resulting in the reproductive success of two transplanted orchids in a botanical garden

Hongfang Zhang¹^,³, Liqiang Li², Zhongjian Liu², Yibo Luo¹^,²^,^*()

1 State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing 100093
2 The National Orchid Conservation Center, Shenzhen, Guangdong 518114
3 Graduate School of the Chinese Academy of Sciences, Beijing 100049

Received:2009-06-15 Accepted:2009-12-16 Online:2010-01-20 Published:2010-01-20
Contact: Yibo Luo

摘要/Abstract

摘要：

迁地保护是防止物种灭绝的有效措施。为了解在新栖息地兰科植物与昆虫之间的关系, 从而为兰科植物的保护提供借鉴, 作者对迁地栽培于深圳市全国兰科植物种质资源保护中心的本地种血叶兰(Ludisia discolor)和外来种银带虾脊兰(Calanthe argenteo-striata)的传粉系统和繁殖策略进行了观察和研究。血叶兰和银带虾脊兰都具有白色小花组成的花序, 且花有距。在开放栽培条件下, 菜粉蝶(Pieris rapae)是这两种兰科植物唯一有效的传粉者。菜粉蝶携带血叶兰和银带虾脊兰花粉块的部位分别是降落时平衡身体的左侧足部和取食花蜜的喙。两种兰科植物传粉效率较高, 花粉移走与沉降的比值分别为1.14:1和0.74:1。血叶兰的结实率在2007和2008两年间无显著差异, 且均高于银带虾脊兰。究其原因, 一方面是由于它为传粉者提供报酬; 另一方面是因为血叶兰的花期与菜粉蝶的盛发期相吻合, 有利于充分利用传粉者; 而银带虾脊兰的花期滞后于菜粉蝶的盛发期, 传粉者缺失使得部分花未能授粉。血叶兰和银带虾脊兰都是高度自交亲和的物种, 但不存在无融合生殖和自动自花授粉的现象; 自交和异交的繁殖成功率无显著差异。这两种兰科植物可能存在由传粉者导致的自交现象。血叶兰和银带虾脊兰在迁地栽培到兰科中心后生长良好, 每年花期都有自然结果的现象, 植株周围有新生幼苗的建立, 种群有逐渐扩大的趋势, 显示出迁地保护的可行性。可以预测若干年后, 这两个种在兰科中心具有建立稳定种群的可能性。

关键词: Ludisia discolor, Calanthe argenteo-striata, 迁地保护, 菜粉蝶, 专化传粉, 传粉效率, 繁殖成功

Abstract

Ex-situ conservation is an effective measure to prevent extinction of species. In order to learn the plant-pollinator relationship in ex situ habitat and shed insights into the ex situ conservation, we studied the pollination system and reproductive strategy of two orchids Ludisia discolor from Wutong Mountains, Shenzhen and Calanthe argenteo-striata from Malipo, Yunnan in the Botanical Garden of the National Orchid Conservation Center (NOCC), Shenzhen, southeastern China in 2007 and 2008. Both species have inflorescences with many white flowers which have spur with a narrow entrance. The butterfly, Pieris rapae, was their solely legitimate pollinators. The pollinaria of L. discolor were carried on the legs of butterflies, while that of C. argenteo-striata on the proboscis. The pollination efficiency in both orchids was high, and the ratio of pollinaria removal to massulae deposition was 1.14:1 and 0.74:1 in L. discolor and C. argenteo-striata, respectively. In 2007 and 2008, fruit set of open pollination in L. discolor was higher than that in C. argenteo-striata, which could be explained by that the former provides rewards to its pollinators while the latter is deceptive, as well as by that the flowering period of L. discolor was synchronized with the abundance of butterflies, which favored the orchid to exploit its pollinators, whereas the flowering period of C. argenteo-striata was lagged behind the outbreak period of butterflies, which made the flowers suffer insufficient pollinator visitation. Both species were self-compatible but there were no spontaneous autogamy or apomixes. The fruit sets of cross-pollination and self-pollination showed no significant difference. Ludisia discolor and C. argenteo-striata grow well after being transplanted to NOCC, and new seedlings were found near parent plants, indicating the population tended to expand, and the ex situ conservation was feasible. It could be predicted that these two orchids have the potential to establish stable populations in NOCC in the future.

Key words: Ludisia discolor, Calanthe argenteo-striata, ex situ conservation, Pieris rapae, specialized pollination, pollination efficiency, reproductive success

张洪芳, 李利强, 刘仲健, 罗毅波 (2010) 菜粉蝶对两种迁地保护的兰科植物传粉和繁殖成功的作用. 生物多样性, 18, 11-18. DOI: 10.3724/SP.J.1003.2010.011.

Hongfang Zhang, Liqiang Li, Zhongjian Liu, Yibo Luo (2010) The butterfly Pieris rapae resulting in the reproductive success of two transplanted orchids in a botanical garden. Biodiversity Science, 18, 11-18. DOI: 10.3724/SP.J.1003.2010.011.

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链接本文: https://www.biodiversity-science.net/CN/10.3724/SP.J.1003.2010.011

https://www.biodiversity-science.net/CN/Y2010/V18/I1/11

图/表 2

图1 血叶兰和银带虾脊兰的花部形态与传粉者。(A)血叶兰的花序; (B)血叶兰的花正面: ac, 药帽; l, 唇瓣; v, 粘盘; se, 距口; (C)菜粉蝶的腿携带血叶兰的花粉块; (D)银带虾脊兰的花序; (E)银带虾脊兰的花正面观: ac, 药帽; sc, 柱头腔; l, 唇瓣; v, 粘盘; se, 距口; pa, 突起; (F)菜粉蝶的喙携带银带虾脊兰的花粉块。

Fig. 1 The floral morphology of Ludisia discolor and Calanthe argenteo-striata, and their pollinators, Pieris rapae. (A) The inflorescence of L. discolor; (B) Front view of L. discolor flower: ac, Anther cap; l, Labellum; v, Viscidium; se, Spur entrance; (C) The pollinaria of L. discolor are carried by the legs of Pieris rapae; (D) The inflorescence of C. argenteo-striata; (E) Front view of C. argenteo-striata flower: ac, anther cap; sc, Stigma cavity; l, Labellum; v, Viscidium; se, Spur entrance; pa, Papilla; (F) The pollinaria of C. argenteo-striata are carried by the proboscis of Pieris rapae.

表1 血叶兰和银带虾脊兰繁育系统实验结果

Table 1 Breeding system of Ludisia discolor and Calanthe argenteo-striata

	花序数 No. of inflorescence	结果率 Fruit set (%)
	花序数 No. of inflorescence	血叶兰 Ludisia discolor	银带虾脊兰 Calanthe argenteo-striata
人工自花授粉 Self-pollination (S)	10	91.47	91.82
人工异花授粉 Outcross-pollination (O)	10	89.77	89.95
套袋 Bagged (B)	10	0	0
去雄 Emasculated and bagged (E)	10	0	0
自然传粉 Open pollination (2007)	20	48.94	17.44
自然传粉 Open pollination (2008)	20	47.18	11.27

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菜粉蝶对两种迁地保护的兰科植物传粉和繁殖成功的作用

The butterfly Pieris rapae resulting in the reproductive success of two transplanted orchids in a botanical garden

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