福建省家鸭的遗传多样性及其与野鸭的亲缘关系

doi:10.3724/SP.J.1003.2008.08179

摘要/Abstract

摘要：

为了探讨福建省家鸭的遗传多样性和品种进化, 本研究测定了福建省4个地方家鸭品种的32个个体和野生斑嘴鸭(Anas zonorhyncha) 8个个体的线粒体DNA D-loop区667 bp序列, 分析了这4个家鸭品种的遗传多态性及品种进化。结果显示4个家鸭D-loop区序列的A、G、C、T碱基平均含量分别为25.5%, 15.2%, 33.5%, 25.8%。确定了8种单倍型, 其中Hap2(与GenBank中的A7一致)为家鸭的主体单倍型, 平均单倍型多样度为0.75202, 平均核苷酸多样度为0.21%。4个品种中金定鸭单倍型多样度、核苷酸多样度最高。4个品种之间的Kimura双参数遗传距离为0.00094-0.00395, 其中连城白鸭和金定鸭之间遗传距离最大。结合GenBank上已发表的绿头鸭(A. platyrhynchos)和斑嘴鸭序列, 共有30种单倍型, 共同构建系统发生树和网络关系图, 结果表明4个家鸭品种单一地起源于绿头鸭。

关键词: 鸭, D-loop区, 遗传多样性, 进化

Abstract

To study the genetic diversity and evolution of four breeds of Fujian domestic ducks, the 667 bp control region (D-loop) of mitochondrial DNA from 32 individual domestic ducks and eight spot-billed ducks (Anas zonorhyncha) were sequenced. The results showed that in the D-loop region of four domestic duck breeds, the content of A, G, C and T nucleotides was 25.5%, 15.2%, 33.5% and 25.8%, respectively. Eight haplotypes were found, in which Hap2 (consistent with haplotype A7 in GenBank) was dominant. The average haplotype diversity (Hd) and nucleotide diversity (Pi) of domestic ducks were 0.75202 and 0.21%, respectively. The Hdand Pi of Jinding duck was the highest among the four domestic duck breeds. Kimura 2-parameter genetic distance among the four duck breeds were 0.00094-0.00395. Combined with the sequences of mallard (A. platyrhynchos) and spot-billed ducks taken from GenBank, a total of 30 haplotypes were used in the phylogenetic analysis, and the results showed that the four domestic duck breeds originated from A. platyrhynchos.

Key words: domestic ducks, D-loop region, genetic diversity, evolution

李慧芳, 徐文娟, 朱文奇, 薛素美, 陈宽维 (2008) 福建省家鸭的遗传多样性及其与野鸭的亲缘关系. 生物多样性, 16, 607-612. DOI: 10.3724/SP.J.1003.2008.08179.

Huifang Li, Wenjuan Xu, Wenqi Zhu, Sumei Xue, Kuanwei Chen (2008) Genetic diversity and evolution of Fujian domestic duck breeds. Biodiversity Science, 16, 607-612. DOI: 10.3724/SP.J.1003.2008.08179.

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链接本文: https://www.biodiversity-science.net/CN/10.3724/SP.J.1003.2008.08179

https://www.biodiversity-science.net/CN/Y2008/V16/I6/607

图/表 4

表1 4个家鸭品种内遗传多样性

Table 1 Genetic diversity in four domestic duck breeds

品种 Breed	样本量 Sample size	单倍型数 No. of haplotypes	单倍型比例 Haplotype ratio (%)	单倍型多样度 Haplotype diversity Hd	平均核苷酸差异数Mean number of nucleotide differences K	核苷酸多样度 Nucleotide diversity Pi
莆田黑鸭 PT	8	3	37.5	0.46429	0.75000	0.00112
金定鸭 JD	8	3	37.5	0.75000	0.96429	0.00145
连城白鸭 LC	8	3	37.5	0.60714	0.78571	0.00118
山麻鸭 SM	8	2	25.0	0.42857	0.42857	0.00064

图1 福建省4个家鸭群体聚类图(选择斑嘴鸭为外群。群体代号同表1)

Fig. 1 A dendrogram of four domestic duck breeds of Fujian based on D-loop sequences. Population codes see Table 1.

图2 基于mtDNA控制区序列, 使用Kimura双参数模型构建的NJ系统发育树

Fig. 2 A phylogenetic tree based on D-loop sequences constructed with NJ method using Kimura’s two-parameter model

图3 鸭线粒体DNA D-loop单倍型间网络结构图(白色圆代表福建家鸭品种的单倍型,黑色圆代表野鸭的单倍型)。

Fig. 3 Reduced median-joining networks of mtDNA D-loop haplotypes. The hollow circles represent the haplotypes of Fujian domestic ducks, and the solid circles represent the haplotypes of mallard.

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