花楸树天然群体的遗传多样性研究

doi:10.3724/SP.J.1003.2008.08201

生物多样性 ›› 2008, Vol. 16 ›› Issue (6): 562-569. DOI: 10.3724/SP.J.1003.2008.08201

花楸树天然群体的遗传多样性研究

郑健¹^,², 郑勇奇¹^,^*(), 张川红¹, 宗亦臣¹, 李伯菁¹, 吴超³

1 中国林业科学研究院林业研究所, 北京 100091
2 中国林业科学研究院热带林业研究所, 广州 510520
3 天津市河东区绿化管理二所, 天津 300176

收稿日期:2008-08-19 接受日期:2008-10-29 出版日期:2008-11-20 发布日期:2008-11-20
通讯作者: 郑勇奇
基金资助:
国家科技支撑计划农业领域课题(2006BAD13B07);国家科技基础条件平台工作课题(2005DKA21003-05)

Genetic diversity in natural populations of Sorbus pohuashanensis

Jian Zheng¹^,², Yongqi Zheng¹^,^*(), Chuanhong Zhang¹, Yichen Zong¹, Bojing Li¹, Chao Wu³

1 Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091
2 Research Institute of Tropic Forestry, Chinese Academy of Forestry, Guangzhou 510520
3 Afforestation Management Office of Hedong District, Tianjin 300176

Received:2008-08-19 Accepted:2008-10-29 Online:2008-11-20 Published:2008-11-20
Contact: Yongqi Zheng
About author:* E-mail: zhengyq@caf.ac.cn

摘要/Abstract

摘要：

花楸树(Sorbus pohuashanensis)是我国北方一种观赏兼经济用途的树种。本研究采用水平淀粉凝胶同工酶电泳技术, 对采自山东、山西、河北、辽宁4个省的8个花楸树天然群体的种子样本进行了分析, 旨在了解花楸树天然群体的遗传多样性和遗传结构, 为该树种的保护与利用提供科学依据。4个酶系统10个位点的检测结果表明, 花楸树群体水平上的遗传多样性较高, 每位点平均等位基因数(N_a)为2.2000, 多态位点百分率(P)为100%, 期望杂合度(H_e)为0.4240。花楸树8个群体间的有效等位基因数(N_e)、H_e和Shannon信息指数差异较小,3个指标从高到低依次为: 河北驼梁山>河北雾灵山>山西庞泉沟>河北白石山>山东崂山>河北塞罕坝>山东泰山>辽宁老秃顶子。群体间遗传分化系数(F_st)为0.0758, 群体间总的基因流较高(N_m = 3.0472), 群体间遗传一致度较高(I为0.8585- 0.9872), 表明群体间遗传分化程度小。在单个群体中, 通过χ²检验, 花楸树群体有73.62%的位点组合显著偏离Hardy-Weinberg平衡(P<0.05), 总群体水平近交系数(F_it)和单个群体水平近交系数(F_is)分别为-0.3105和-0.4180, 表明无论在总体水平还是群体内个体间, 花楸树群体表现为杂合体过量的现象。UPGMA聚类结果显示, 8个群体的遗传距离与地理距离相关性不显著。

关键词: Sorbus pohuashanensis, 同工酶, 遗传多样性, 遗传结构, 遗传分化

Abstract

Sorbus pohuashanensis is a tree species native to northern China with high potential for ornamental use. The genetic structure and diversity of eight natural populations of Sorbus pohuashanensis were studied at ten loci encoding four isozymes using horizontal starch gel electrophoresis. Genetic diversity was high at the species level with the mean number of alleles per locus N_a= 2.2000, the percentage of polymorphic loci P = 100%, and the mean expected heterozygosity H_e= 0.4240. Values of the three parameters of H_e, N_e, and Shannon′s information index indicated a random pattern of genetic diversity among the eight populations. A low level of genetic differentiation among populations (F_st= 0.0758) and a high estimate of gene flow (N_m= 3.0472) were detected together with a high level of genetic identity (I) among the populations (from 0.8585 to 0.9872). Wright’sF-statistics analysis indicated an excess of heterozygotes both at the individual population level (F_is= -0.4180) and at the total population level (F_it= -0.3105). χ²-tests (P<0.05) indicated that 73.62% of the assayed loci departed from the Hardy-Weinberg equilibrium. The UPGMA cluster analysis suggested that the genetic distances among populations were weakly correlated with their geographic distances.

Key words: Sorbus pohuashanensis, isozyme, genetic diversity, genetic structure, genetic differentiation

郑健, 郑勇奇, 张川红, 宗亦臣, 李伯菁, 吴超 (2008) 花楸树天然群体的遗传多样性研究. 生物多样性, 16, 562-569. DOI: 10.3724/SP.J.1003.2008.08201.

Jian Zheng, Yongqi Zheng, Chuanhong Zhang, Yichen Zong, Bojing Li, Chao Wu (2008) Genetic diversity in natural populations of Sorbus pohuashanensis. Biodiversity Science, 16, 562-569. DOI: 10.3724/SP.J.1003.2008.08201.

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链接本文: https://www.biodiversity-science.net/CN/10.3724/SP.J.1003.2008.08201

https://www.biodiversity-science.net/CN/Y2008/V16/I6/562

图/表 7

参考文献 29

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群体代号 Population code	采样地 Seed collection site	经纬度 Geographic location	海拔 Altitude (m)	家系数量 No. of families	每家系种子数 No. of seeds per family	样本大小 Sample size
SDL	山东崂山 Laoshan, Shandong	120°37.5′E 36°11′N	493-991	12	30	360
SDT	山东泰山 Taishan, Shandong	117°06.1′E 36°15.8′N	1,002-1,451	30	10	300
SXP	山西庞泉沟 Pangquangou, Shanxi	111°25.7′E 37°53.6′N	1,834-2,179	12	30	360
HBB	河北白石山 Baishishan, Hebei	114°41.0′E 39°13.2′N	1,654-1,931	12	30	360
HBT	河北驼梁山 Tuoliangshan, Hebei	113°49.5′E 38°45.1′N	1,564-1,971	30	8	240
HBW	河北雾灵山 Wulingshan, Hebei	117°35.0′E 40°38.0′N	809-1,847	30	8	240
HBS	河北塞罕坝 Saihanba, Hebei	117°39.0′E 42°36.0′N	1,287-1,674	30	8	240
LNL	辽宁老秃顶子 Laotudingzi, Liaoning	124°53.9′E 41°19.2′N	890-1,245	12	30	360

群体代号 Population code	采样地 Seed collection site	经纬度 Geographic location	海拔 Altitude (m)	家系数量 No. of families	每家系种子数 No. of seeds per family	样本大小 Sample size
SDL	山东崂山 Laoshan, Shandong	120°37.5′E 36°11′N	493-991	12	30	360
SDT	山东泰山 Taishan, Shandong	117°06.1′E 36°15.8′N	1,002-1,451	30	10	300
SXP	山西庞泉沟 Pangquangou, Shanxi	111°25.7′E 37°53.6′N	1,834-2,179	12	30	360
HBB	河北白石山 Baishishan, Hebei	114°41.0′E 39°13.2′N	1,654-1,931	12	30	360
HBT	河北驼梁山 Tuoliangshan, Hebei	113°49.5′E 38°45.1′N	1,564-1,971	30	8	240
HBW	河北雾灵山 Wulingshan, Hebei	117°35.0′E 40°38.0′N	809-1,847	30	8	240
HBS	河北塞罕坝 Saihanba, Hebei	117°39.0′E 42°36.0′N	1,287-1,674	30	8	240
LNL	辽宁老秃顶子 Laotudingzi, Liaoning	124°53.9′E 41°19.2′N	890-1,245	12	30	360

位点 Locus	等位基因 Allele	等位基因频率 Allele frequencies
位点 Locus	等位基因 Allele	HBW	HBS	HBT	HBB	SDL	SDT	SXP	LNL
Pgm-1	a	0.3229	0.3500	0.2497	0.1958	0.2458	0.2467	0.2917	0.4889
	b	0.4521	0.3667	0.4979	0.4181	0.4222	0.3433	0.4486	0.2278
	c	0.2550	0.2833	0.2542	0.3861	0.3319	0.4100	0.2597	0.2833
Pgm-2	a	0.6917	0.9417	0.4250	0.8194	0.1736	0.2233	0.7181	0.7514
Pgm-2	b	0.3083	0.0583	0.5750	0.1806	0.8264	0.7767	0.2819	0.2486
Pgm-3	a	0.6917	0.7396	0.5021	0.8986	0.3764	0.6167	0.9361	0.9333
Pgm-3	b	0.3083	0.2604	0.4979	0.1014	0.6236	0.3833	0.0639	0.0667
Pgi-1	a	0.0167	0.0125	0.0854	0.0181	0.0556	0.0000	0.0361	0.0153
	b	0.1437	0.2167	0.1187	0.1889	0.3889	0.2500	0.2153	0.2708
	c	0.8396	0.7708	0.7958	0.7931	0.5556	0.7500	0.7486	0.7139
Pgi-2	a	0.6854	0.9500	0.5583	0.6611	0.8333	0.9450	0.6264	0.7194
Pgi-2	b	0.3146	0.0500	0.4417	0.3389	0.1667	0.0550	0.3736	0.2806
Pgd-1	a	0.7562	0.6771	0.8729	0.9069	0.9347	0.9867	0.6917	0.9653
Pgd-1	b	0.2437	0.3229	0.1271	0.0931	0.0653	0.0133	0.3083	0.0347
Pgd-2	a	0.0375	0.1562	0.0042	0.1861	0.0000	0.2050	0.0278	0.1181
Pgd-2	b	0.9625	0.8438	0.9958	0.8139	1.0000	0.7950	0.9722	0.8819
Pgd-3	a	0.5104	0.5250	0.5104	0.5194	0.5056	0.5000	0.5000	0.5014
Pgd-3	b	0.4896	0.4750	0.4896	0.4806	0.4944	0.5000	0.5000	0.4986
Mdh-1	a	0.5000	0.5000	0.5000	0.5000	0.5000	0.5000	0.5000	0.5000
Mdh-1	b	0.5000	0.5000	0.5000	0.5000	0.5000	0.5000	0.5000	0.5000
Mdh-2	a	0.5000	0.5000	0.5000	0.5000	0.5000	0.5000	0.5000	0.5000
Mdh-2	b	0.5000	0.5000	0.5000	0.5000	0.5000	0.5000	0.5000	0.5000

位点 Locus	等位基因 Allele	等位基因频率 Allele frequencies
位点 Locus	等位基因 Allele	HBW	HBS	HBT	HBB	SDL	SDT	SXP	LNL
Pgm-1	a	0.3229	0.3500	0.2497	0.1958	0.2458	0.2467	0.2917	0.4889
	b	0.4521	0.3667	0.4979	0.4181	0.4222	0.3433	0.4486	0.2278
	c	0.2550	0.2833	0.2542	0.3861	0.3319	0.4100	0.2597	0.2833
Pgm-2	a	0.6917	0.9417	0.4250	0.8194	0.1736	0.2233	0.7181	0.7514
Pgm-2	b	0.3083	0.0583	0.5750	0.1806	0.8264	0.7767	0.2819	0.2486
Pgm-3	a	0.6917	0.7396	0.5021	0.8986	0.3764	0.6167	0.9361	0.9333
Pgm-3	b	0.3083	0.2604	0.4979	0.1014	0.6236	0.3833	0.0639	0.0667
Pgi-1	a	0.0167	0.0125	0.0854	0.0181	0.0556	0.0000	0.0361	0.0153
	b	0.1437	0.2167	0.1187	0.1889	0.3889	0.2500	0.2153	0.2708
	c	0.8396	0.7708	0.7958	0.7931	0.5556	0.7500	0.7486	0.7139
Pgi-2	a	0.6854	0.9500	0.5583	0.6611	0.8333	0.9450	0.6264	0.7194
Pgi-2	b	0.3146	0.0500	0.4417	0.3389	0.1667	0.0550	0.3736	0.2806
Pgd-1	a	0.7562	0.6771	0.8729	0.9069	0.9347	0.9867	0.6917	0.9653
Pgd-1	b	0.2437	0.3229	0.1271	0.0931	0.0653	0.0133	0.3083	0.0347
Pgd-2	a	0.0375	0.1562	0.0042	0.1861	0.0000	0.2050	0.0278	0.1181
Pgd-2	b	0.9625	0.8438	0.9958	0.8139	1.0000	0.7950	0.9722	0.8819
Pgd-3	a	0.5104	0.5250	0.5104	0.5194	0.5056	0.5000	0.5000	0.5014
Pgd-3	b	0.4896	0.4750	0.4896	0.4806	0.4944	0.5000	0.5000	0.4986
Mdh-1	a	0.5000	0.5000	0.5000	0.5000	0.5000	0.5000	0.5000	0.5000
Mdh-1	b	0.5000	0.5000	0.5000	0.5000	0.5000	0.5000	0.5000	0.5000
Mdh-2	a	0.5000	0.5000	0.5000	0.5000	0.5000	0.5000	0.5000	0.5000
Mdh-2	b	0.5000	0.5000	0.5000	0.5000	0.5000	0.5000	0.5000	0.5000

群体 Population	样本数量 Sample size	N_a	N_e	P (%)	H_o	H_e	I_s
河北雾灵山 HBW	240	2.2000	1.8068	100.00	0.5717	0.4148	0.6206
河北塞罕坝 HBS	240	2.2000	1.7510	100.00	0.5500	0.3819	0.5814
河北驼梁山 HBT	240	2.2000	1.8423	100.00	0.5771	0.4192	0.6234
河北白石山 HBB	360	2.2000	1.7854	100.00	0.5436	0.3875	0.5932
山东崂山 SDL	360	2.1000	1.7837	90.00	0.5342	0.3850	0.5824
山东泰山 SDT	300	2.1000	1.7538	100.00	0.5403	0.3810	0.5707
山西庞泉沟 SXP	360	2.2000	1.7968	100.00	0.5900	0.4017	0.6054
辽宁老秃顶子 LNL	360	2.2000	1.7159	100.00	0.5367	0.3728	0.5695
平均 Mean	308	2.1750	1.7732	98.75	0.5555	0.3930	0.5933

花楸树天然群体的遗传多样性研究

Genetic diversity in natural populations of Sorbus pohuashanensis

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位点 Locus	F_is	F_it	F_st	N_m
Pgm-1	-0.1943	-0.1631	0.0261	9.3373
Pgm-2	0.3419	0.5342	0.2921	0.6057
Pgm-3	0.3813	0.4948	0.1834	1.1130
Pgi-1	-0.2672	-0.2245	0.0337	7.1665
Pgi-2	-0.4712	-0.3251	0.0993	2.2685
Pgd-1	0.0148	0.1172	0.1040	2.1546
Pgd-2	-0.1133	-0.0304	0.0745	3.1065
Pgd-3	-0.9505	-0.9499	0.0003	****
Mdh-1	-1.0000	-1.0000	0.0000	****
Mdh-2	-1.0000	-1.0000	0.0000	****
平均 Mean	-0.4180	-0.3105	0.0758	3.0472

	HBW	HBS	HBT	HBB	SDL	SDT	SXP	LNL
HBW	****	0.9728	0.9750	0.9788	0.9165	0.9325	0.9872	0.9726
HBS	0.0276	****	0.9071	0.9681	0.8585	0.8959	0.9646	0.9624
HBT	0.0253	0.0975	****	0.9374	0.9619	0.9515	0.9456	0.9307
HBB	0.0214	0.0324	0.0646	****	0.8687	0.9140	0.9837	0.9855
SDL	0.0872	0.1526	0.0388	0.1408	****	0.9751	0.8765	0.8807
SDT	0.0699	0.1099	0.0497	0.0899	0.0252	****	0.9040	0.9226
SXP	0.0129	0.0361	0.0559	0.0164	0.1318	0.1010	****	0.9776
LNL	0.0278	0.0383	0.0719	0.0146	0.1270	0.0806	0.0227	****

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