内蒙草原不同植物功能群及物种对土壤微生物组成的影响

doi:10.3724/SP.J.1003.2012.07160

生物多样性 ›› 2012, Vol. 20 ›› Issue (1): 59-65. DOI: 10.3724/SP.J.1003.2012.07160

所属专题：土壤生物与土壤健康

内蒙草原不同植物功能群及物种对土壤微生物组成的影响

陈颖¹^,², 李肖肖³, 应娇妍²^,^*(), 梁存柱¹, 白永飞²

1 内蒙古大学生命科学学院, 中美生态、能源及可持续性科学内蒙古研究中心, 呼和浩特 010021
2 中国科学院植物研究所植被与环境变化国家重点实验室, 北京 100093
3 中国科学院生态环境研究中心, 北京 100085

收稿日期:2011-09-13 接受日期:2012-01-12 出版日期:2012-01-20 发布日期:2012-02-14
通讯作者: 应娇妍
作者简介:*E-mail: yingjy@ibcas.ac.cn
基金资助:
国家重点基础研究发展计划项目(2007CB106800);国家自然科学基金面上项目(31170404)

Effects of plant functional groups and plant species on soil microbial composition in a Inner Mongolian grassland

Ying Chen¹^,², Xiaoxiao Li³, Jiaoyan Ying²^,^*(), Cunzhu Liang¹, Yongfei Bai²

1 College of Life Science, Inner Mongolia University, Sino-US Center for Conservation, Energy and Sustainability Science in Inner Mongolia, Hohhot 010021
2 State Key Laboratory of Vegetation and Environmental Change, Institute of Botany, Chinese Academy of Sciences, Beijing 100093
3 Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085

Received:2011-09-13 Accepted:2012-01-12 Online:2012-01-20 Published:2012-02-14
Contact: Jiaoyan Ying

摘要/Abstract

摘要：

为了分析不同植物群落组成对内蒙古典型草原土壤微生物群落组成的影响, 本研究利用植物功能群剔除处理实验平台, 采用荧光定量PCR(real-time PCR)和自动核糖体间隔区基因分析(automated ribosomal intergenic spacer analysis, ARISA)技术, 对不同植物功能群组成的非根际土壤和常见物种的根际土壤中细菌和真菌的数量及群落结构进行了分析。结果表明, 在非根际土壤中, 不同植物功能群组成对细菌数量有显著影响, 而对真菌数量及细菌和真菌的群落结构影响不明显; 在根际土壤中, 不同植物物种对细菌、真菌的数量都有显著影响。此外, 聚类分析表明, 不同物种的根际土中细菌和真菌的群落结构也有所不同, 尤其以细菌的群落结构变化较为明显。研究结果表明不同植物物种可以通过根系影响土壤微生物群落组成。

关键词: 植物多样性, 根际微生物, real-time PCR, ARISA

Abstract

In order to study the effect of plant community composition on soil microbial communities in a typical Inner Mongolian grassland, the abundance and community structure of bacteria and fungi in bulk soils under different plant functional groups and in soils from the rhizosphere of different grass species were analyzed using real-time PCR and automated ribosomal intergenic spacer analysis (ARISA) under plant functional group removal treatments. Our results indicated that changes in plant functional group affect bacterial abundance, but not fungal abundance or bacterial/fungal community structure in bulk soil. The abundance of bacteria and fungi in the rhizosphere soil varied with plant species. Cluster analysis revealed that community structure of bacteria and fungi also varied among plant species in the rhizosphere soil but not in the bulk soil. which was more evident for bacteria. Our results point to the ways in which plant species can influence soil microbial and fungal communities through the action of their roots.

Key words: plant diversity, rhizosphere microorganisms, real-time PCR, ARISA

陈颖, 李肖肖, 应娇妍, 梁存柱, 白永飞 (2012) 内蒙草原不同植物功能群及物种对土壤微生物组成的影响. 生物多样性, 20, 59-65. DOI: 10.3724/SP.J.1003.2012.07160.

Ying Chen, Xiaoxiao Li, Jiaoyan Ying, Cunzhu Liang, Yongfei Bai (2012) Effects of plant functional groups and plant species on soil microbial composition in a Inner Mongolian grassland. Biodiversity Science, 20, 59-65. DOI: 10.3724/SP.J.1003.2012.07160.

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链接本文: https://www.biodiversity-science.net/CN/10.3724/SP.J.1003.2012.07160

https://www.biodiversity-science.net/CN/Y2012/V20/I1/59

图/表 4

图1 不同植物物种根际土中细菌、真菌的数量。误差线上方的字母分别表示细菌、真菌不同处理间的显著性差异, P < 0.05。

Fig. 1 Abundance of bacteria and fungi in rhizosphere soil of different grass species. Letters above bars indicate significance (P < 0.05) among different treatments 1, Leymus chinensis; 2, Stipa grandis; 3, Agropyron cristatum; 4, Achnatherum sibiricum; 5, Cleistogenes squarrosa.

图2 不同功能群剔除处理下土壤中细菌和真菌的数量。CK: 不剔除; PR: 保留多年生根茎禾草; PB: 保留多年生丛生禾草; BL: 剔除所有植物。误差线上方的字母表示不同处理间的显著性差异(P < 0.05)。

Fig. 2 Abundance of bacteria and fungi under different plant functional group removal treatments. CK, No removal; PR, The remaining group is perennial rhizome forbs; PB, The remaining group is perennial bunch-grass; BL, All the plants were removed. Letters above bars indicate significance (P< 0.05) among treatments.

图3 不同植物物种根际土中细菌群落(A)和真菌群落(B)的ARISA数据的聚类分析

Fig. 3 Cluster analysis of soil bacterial (A) and fungal(B) community structure in rhizosphere soil of different grass species based on ARISA data.

图4 不同功能群剔除小区细菌(A)和真菌(B)群落ARISA数据的聚类分析。CK: 不剔除; PR: 保留多年生根茎禾草; PB: 保留多年生丛生禾草; BL: 剔除所有植物。

Fig. 4 Cluster analysis of soil bacterial (A) and fungal (B) community structure under different functional group removal treatments based on ARISA data. CK, No removal; PR, The remaining group was perennial rhizome forbs; PB, The remaining group was perennial bunch-grass; BL, All the plants were removed.

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内蒙草原不同植物功能群及物种对土壤微生物组成的影响

Effects of plant functional groups and plant species on soil microbial composition in a Inner Mongolian grassland

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