舌鳎亚科鱼类单系起源和同种异名的线粒体DNA证据

doi:10.3724/SP.J.1003.2010.275

生物多样性 ›› 2010, Vol. 18 ›› Issue (3): 275-282. DOI: 10.3724/SP.J.1003.2010.275

舌鳎亚科鱼类单系起源和同种异名的线粒体DNA证据

柳淑芳¹, 刘进贤²^,³, 庄志猛¹^,^*(), 高天翔³, 韩志强⁴, 陈大刚³

1 山东省渔业资源与生态环境重点实验室, 中国水产科学研究院黄海水产研究所, 山东青岛 266071
2 Department of Ecology, University of California, Irvine, CA 92697, USA
3 中国海洋大学, 山东青岛 266100
4 浙江海洋学院, 浙江舟山 316004

收稿日期:2009-10-21 接受日期:2010-01-25 出版日期:2010-05-20 发布日期:2012-02-08
通讯作者: 庄志猛
作者简介:*E-mail: zhuangzm@ysfri.ac.cn
基金资助:
国家自然科学基金(30871913);国家自然科学基金(30271027)

Monophyletic origin and synonymic phenomena in the sub-family Cynoglossinae inferred from mitochondrial DNA sequences

Shufang Liu¹, Jinxian Liu²^,³, Zhimeng Zhuang¹^,^*(), Tianxiang Gao³, Zhiqiang Han⁴, Dagang Chen³

1 Key Laboratory for Fishery Resources and Eco-environment, Shandong Province, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, Shandong, China
2 Department of Ecology, University of California, Irvine, CA 92697, USA
3 Ocean University of China, Qingdao 266100, Shandong, China
4 Zhejiang Ocean University, Zhoushan 316004, Zhejiang, China

Received:2009-10-21 Accepted:2010-01-25 Online:2010-05-20 Published:2012-02-08
Contact: Zhimeng Zhuang

摘要/Abstract

摘要：

舌鳎亚科鱼类因形态特征的特殊性, 其分类及系统发育关系一直存在争议。本研究测定了中国沿海14种舌鳎亚科鱼类线粒体DNA的16S rRNA和Cyt b基因的部分片段。两个基因构建的舌鳎亚科系统发育树结果显示, 中国沿海舌鳎亚科鱼类为明显的单系群, 但舌鳎亚科鱼类内部的系统发育关系与形态分类划分的亚属并不完全一致, 日本须鳎(Paraplagusis japonica)与其他舌鳎属(Cynoglossus)种类并未形成不同的分支。虽然舌鳎属内的舌鳎亚属(Cynoglossus)、拟舌鳎亚属(Cynoglossoides)和三线舌鳎亚属(Areliscus)均可以聚为独立分支, 但长吻红舌鳎(C. lighti)与短吻红舌鳎(C. joyneri)、短吻三线舌鳎(C. abbreviatus)与紫斑舌鳎(C. purpureomaculatus)、半滑舌鳎(C. semilaevis)与窄体舌鳎(C. gracilis)及褐斑三线舌鳎(C. trigrammus)这三组物种可能存在同种异名现象。这一结果提示, 基于形态学对舌鳎亚科的种属分类鉴定尚存在不足, 线粒体DNA的系统发育关系可为其分类的修订提供有意义的参考和佐证。

关键词: 舌鳎亚科, 16S rRNA, Cyt b, 分子系统发育

Abstract

Morphological taxonomy in the fish sub-family Cynoglossinae is complicated and contentious. To clarify these ambiguities, the molecular phylogenic relationships among 14 Cynoglossinae species were studied by analyzing partial 16S rRNA and Cyt b mitochondrial gene sequences. Similar phylogentic topology inferred from both gene fragments demonstrated that Cynoglossinae is a monophyletic group. However, the phylogenetic relationships revealed by the mitochondrial DNA sequence analyses were not consistent with those inferred from morphological classification. In contrast with morphological classification, reciprocal monophyly was not detected between Paraplagusis japonica and the species in the genus Cynoglossus. Neighbor-Joining (NJ) and Maximum Parsimony (MP) trees showed that those species in the three sub-genera Cynoglossus, Cynoglossoides and Areliscus clustered on an independent branch. Also, synonymic phenomena possibly existed in the following three groups of species: C. lighti andC. joyner, C. abbreviatus and C. purpureomaculatus, as well as in C. semilaevis, C. gracilis and C. trigrammus. Our results highlight the open questions surrounding the morphological classification of Cynoglossinae species. Information on molecular phylogenic relationships will likely be of help in revising the morphological classification of Cynoglossinae species.

Key words: Cynoglossinae, 16S rRNA, Cyt b, molecular phylogenic relationship

柳淑芳, 刘进贤, 庄志猛, 高天翔, 韩志强, 陈大刚 (2010) 舌鳎亚科鱼类单系起源和同种异名的线粒体DNA证据. 生物多样性, 18, 275-282. DOI: 10.3724/SP.J.1003.2010.275.

Shufang Liu, Jinxian Liu, Zhimeng Zhuang, Tianxiang Gao, Zhiqiang Han, Dagang Chen (2010) Monophyletic origin and synonymic phenomena in the sub-family Cynoglossinae inferred from mitochondrial DNA sequences. Biodiversity Science, 18, 275-282. DOI: 10.3724/SP.J.1003.2010.275.

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https://www.biodiversity-science.net/CN/Y2010/V18/I3/275

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参考文献 22

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种名 Species	亚属名 Sub-genus	样本量 Sample size	Cytb测定数 No. of Cytb DNA	16S rRNA测定数 No. of 16S rRNA
内群 Ingroup
1 双线舌鳎 Cynoglossus bilineatus	Arelia	2	2	1
2 中华舌鳎 C. sinicus	Cynoglossus	2	1	1
3 褐斑三线舌鳎 C. trigrammus	Areliscus	1	1	1
4 窄体舌鳎 C. gracilis	Areliscus	2	1	1
5 长吻红舌鳎 C. lighti	Areliscus	2	2	1
6 短吻红舌鳎 C. joyneri	Areliscus	2	2	1
7 黑鳃舌鳎 C. roulei	Areliscus	1	1	1
8 紫斑舌鳎 C. purpureomaculatus	Areliscus	2	1	1
9 短吻三线舌鳎 C. abbreviatus	Areliscus	5	2	2
10 半滑舌鳎 C. semilaevis	Areliscus	3	2	1
11 斑头舌鳎 C. puncticeps	Cynoglossoides	1	1	1
12 少鳞舌鳎 C. oligolepis	Cynoglossoides	2	2	1
13 宽体舌鳎 C. robustus	Cynoglossoides	2	1	1
14 日本须鳎 Paraplagusis japonica	Rhinoplagusia	1	1	1
外群 Outgroup
15 塞内加尔鳎 Sole senegalensis		3	3	2
16 人字钩嘴鳎 Heteromycteris matsubarai		2	1	2
17 圆斑星鲽 Verasper variegatus		1	1	1

种名 Species	亚属名 Sub-genus	样本量 Sample size	Cytb测定数 No. of Cytb DNA	16S rRNA测定数 No. of 16S rRNA
内群 Ingroup
1 双线舌鳎 Cynoglossus bilineatus	Arelia	2	2	1
2 中华舌鳎 C. sinicus	Cynoglossus	2	1	1
3 褐斑三线舌鳎 C. trigrammus	Areliscus	1	1	1
4 窄体舌鳎 C. gracilis	Areliscus	2	1	1
5 长吻红舌鳎 C. lighti	Areliscus	2	2	1
6 短吻红舌鳎 C. joyneri	Areliscus	2	2	1
7 黑鳃舌鳎 C. roulei	Areliscus	1	1	1
8 紫斑舌鳎 C. purpureomaculatus	Areliscus	2	1	1
9 短吻三线舌鳎 C. abbreviatus	Areliscus	5	2	2
10 半滑舌鳎 C. semilaevis	Areliscus	3	2	1
11 斑头舌鳎 C. puncticeps	Cynoglossoides	1	1	1
12 少鳞舌鳎 C. oligolepis	Cynoglossoides	2	2	1
13 宽体舌鳎 C. robustus	Cynoglossoides	2	1	1
14 日本须鳎 Paraplagusis japonica	Rhinoplagusia	1	1	1
外群 Outgroup
15 塞内加尔鳎 Sole senegalensis		3	3	2
16 人字钩嘴鳎 Heteromycteris matsubarai		2	1	2
17 圆斑星鲽 Verasper variegatus		1	1	1

舌鳎亚科鱼类单系起源和同种异名的线粒体DNA证据

Monophyletic origin and synonymic phenomena in the sub-family Cynoglossinae inferred from mitochondrial DNA sequences

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