用于蜜蜂和熊蜂肠道微生物分类的细菌16S rRNA数据库优化

doi:10.17520/biods.2019021

生物多样性 ›› 2019, Vol. 27 ›› Issue (5): 557-566. DOI: 10.17520/biods.2019021

用于蜜蜂和熊蜂肠道微生物分类的细菌16S rRNA数据库优化

张雪¹,李兴安²,苏秦之¹,曹棋钠¹,李晨伊¹,牛庆生^2,^*(),郑浩^1,^*()

1 北京市食品营养与人类健康高精尖创新中心, 中国农业大学食品科学与营养工程学院, 北京 100083
2 吉林省养蜂科学研究所蜜蜂遗传育种省重点实验室, 吉林省吉林市 132108

收稿日期:2019-01-25 接受日期:2019-05-07 出版日期:2019-05-20 发布日期:2019-05-20
通讯作者: 牛庆生,郑浩
基金资助:
国家自然科学基金(31870472;31470123)

A curated 16S rRNA reference database for the classification of honeybee and bumblebee gut microbiota

Zhang Xue¹,Li Xing’an²,Su Qinzhi¹,Cao Qina¹,Li Chenyi¹,Niu Qingsheng^2,^*(),Zheng Hao^1,^*()

1 Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083
2 Provincial Key Laboratory of Bee Genetics and Breeding, Apiculture Science Institute of Jilin Province, Jilin City, Jilin Province 132108

Received:2019-01-25 Accepted:2019-05-07 Online:2019-05-20 Published:2019-05-20
Contact: Niu Qingsheng,Zheng Hao

摘要/Abstract

摘要：

蜜蜂和熊蜂是重要的传粉昆虫, 对农业生产及生态平衡的维持具有重要作用。近年来, 研究发现蜜蜂及熊蜂肠道内含有大量微生物, 其组成简单、特异。正常的肠道微生物群落对蜜蜂的生长、激素调节、致病菌抵抗等具有重要作用。随着高通量测序的发展, 研究者们也可快速获得传粉蜂肠道微生物组成, 这给生物多样性和物种保护及蜂类健康等的研究带来了便捷。但是由于蜜蜂和熊蜂肠道微生物群落均由特殊菌种组成, 目前的细菌16S rRNA数据库无法对其进行准确的分类, 并且部分东方蜜蜂(Apis cerana)特有的肠道微生物菌种缺乏16S rRNA序列信息。本文从来源于5个不同省份的东方蜜蜂肠道中分离得到在东方蜜蜂中普遍含有的Apibacter菌属纯菌, 获取其全长16S rRNA序列, 并对目前蜜蜂和熊蜂肠道的5个核心菌种的分类进行了综述, 对其分类和命名进行了修正。根据蜜蜂肠道微生物的明确分类, 在目前常用的SILVA细菌分类数据库基础之上对其进行了命名及分类优化, 并加入东方蜜蜂中普遍含有的Apibacter序列, 从而获得了优化数据库Bee Gut Microbiota-Database (BGM-Db)。通过1组东方蜜峰及1组西方蜜蜂(Apis mellifera)的肠道菌群高通量测序结果, 分析不同数据库的表现, 我们发现相比于SILVA和Ribosomal Database Project (RDP), BGM-Db对蜜蜂肠道16S rRNA高通量测序短序列实现了菌种级别的分类, 分辨率更高。

关键词: 蜜蜂, 肠道微生物, 高通量测序, 数据库, 16S rRNA

Abstract

Honey and bumble bees are import pollinators, playing significant roles in the agricultural industry and maintaining the bio-ecosystem balance. Recently, it was found that the bees harbor a simple, yet specific gut microbiota. The normal bee gut microbiota makes essential contributions to host growth, endocrine signaling, and pathogen resistance. With the development of high through-put sequencing technology, researchers can now quickly identify the gut community structure for a low cost. This is helpful for biodiversity, conservation and bee health studies. However, the currently-used 16S rRNA databases are not specific enough to classify the bee gut microbiota properly. Many of the specific bacteria that enrich the gut of Apis cerana are in the genus Apibacter. Here, we isolated Apibacter species from A. cerana collected in five provinces of China, and added them to the current SILVA database. We also curated the nomenclature of some existing sequences and re-classified them in the updated database. Based on the analysis of the 16S rRNA sequencing data from one A. cerana and one Apis mellifera sample, our Bee Gut Microbiota-Database (BGM-Db) offers a more accurate classification of bee gut microbiota at a higher resolution than either the SILVA or Ribosomal Database Project (RDP) database.

Key words: honey bee, gut microbiota, high-throughput sequencing, database, 16S rRNA

张雪, 李兴安, 苏秦之, 曹棋钠, 李晨伊, 牛庆生, 郑浩 (2019) 用于蜜蜂和熊蜂肠道微生物分类的细菌16S rRNA数据库优化. 生物多样性, 27, 557-566. DOI: 10.17520/biods.2019021.

Zhang Xue, Li Xing’an, Su Qinzhi, Cao Qina, Li Chenyi, Niu Qingsheng, Zheng Hao (2019) A curated 16S rRNA reference database for the classification of honeybee and bumblebee gut microbiota. Biodiversity Science, 27, 557-566. DOI: 10.17520/biods.2019021.

导出引用管理器 EndNote|Ris|BibTeX

链接本文: https://www.biodiversity-science.net/CN/10.17520/biods.2019021

https://www.biodiversity-science.net/CN/Y2019/V27/I5/557

图/表 3

参考文献 44

[1]	Asama T, Arima TH, Gomi T, Keishi T, Tani H, Kimura Y, Tatefuji T, Hashimoto K ( 2015) Lactobacillus kunkeei YB38 from honeybee products enhances IgA production in healthy adults. Journal of Applied Microbiology, 119, 818-826. DOI URL
[2]	Bottacini F, Milani C, Turroni F, Sánchez B, Foroni E, Duranti S, Serafini F, Viappiani A, Strati F, Ferrarini A, Delledonne M, Henrissat B, Coutinho P, Fitzgerald GF, Margolles A, van Sinderen D, Ventura M ( 2012) Bifidobacterium asteroides PRL2011 genome analysis reveals clues for colonization of the insect gut. PLoS ONE, 7, e44229. DOI URL
[3]	Cole JR, Wang Q, Fish JA, Chai B, McGarrell DM, Sun Y, Brown CT, Porras-Alfaro A, Kuske CR, Tiedje JM ( 2014) Ribosomal Database Project: Data and tools for high throughput rRNA analysis. Nucleic Acids Research, 42, D633-D642. DOI URL
[4]	Corby-Harris V, Snyder L, Meador CAD, Naldo R, Mott B, Anderson KE ( 2016) Parasaccharibacter apium, gen. nov., sp. nov., improves honey bee (Hymenoptera: Apidae) resistance to Nosema. Journal of Economic Entomology, 109, 537-543. DOI URL
[5]	Corby-Harris V, Snyder LA, Schwan MR, Maes P, McFrederick QS, Anderson KE ( 2014) Origin and effect of Alpha 2.2 Acetobacteraceae in honey bee larvae and description of Parasaccharibacter apium gen. nov., sp. nov. Applied and Environmental Microbiology, 80, 7460-7472. DOI URL
[6]	Cox-Foster DL, Conlan S, Holmes EC, Palacios G, Evans JD, Moran NA, Quan PL, Briese T, Hornig M, Geiser DM, Martinson V, vanEngelsdorp D, Kalkstein AL, Drysdale A, Hui J, Zhai J, Cui L, Hutchison SK, Simons JF, Egholm M, Pettis JS, Lipkin WI ( 2007) A metagenomic survey of microbes in honey bee colony collapse disorder. Science, 318, 283-287. DOI URL
[7]	Ellegaard KM, Tamarit D, Javelind E, Olofsson TC, Andersson SGE, Vásquez A ( 2015) Extensive intra-phylotype diversity in Lactobacilli and Bifidobacteria from the honeybee gut. BMC Genomics, 16, 284. DOI URL
[8]	Engel P, Bartlett KD, Moran NA ( 2015) The bacterium Frischella perrara causes scab formation in the gut of its honeybee host. mBio, 6, e00193-15.
[9]	Engel P, Kwong WK, Moran NA ( 2013) Frischella perrara gen. nov., sp. nov., a Gammaproteobacterium isolated from the gut of the honeybee, Apis mellifera. International Journal of Systematic and Evolutionary Microbiology, 63, 3646-3651.
[10]	Engel P, Martinson VG, Moran NA ( 2012) Functional diversity within the simple gut microbiota of the honey bee. Proceedings of the National Academy of Sciences, USA, 109, 11002-11007. DOI URL
[11]	Engel P, Stepanauskas R, Moran NA ( 2014) Hidden diversity in honey bee gut symbionts detected by single-cell genomics. PLoS Genetics, 10, e1004596. DOI URL
[12]	Killer J, Kopečný J, Mrázek J, Havlík J, Koppová I, Benada O, Rada V, Kofroňová O ( 2010) Bombiscardovia coagulans gen. nov., sp. nov., a new member of the family Bifidobacteriaceae isolated from the digestive tract of bumblebees. Systematic and Applied Microbiology, 33, 359-366. DOI URL
[13]	Killer J, Kopecny J, Mrazek J, Koppova I, Havlik J, Benada O, Kott T ( 2011) Bifidobacterium actinocoloniiforme sp. nov. and Bifidobacterium bohemicum sp. nov., from the bumblebee digestive tract. International Journal of Systematic and Evolutionary Microbiology, 61, 1315-1321. DOI URL
[14]	Killer J, Kopecny J, Mrazek J, Rada V, Benada O, Koppova I, Havlik J, Straka J ( 2009) Bifidobacterium bombi sp. nov., from the bumblebee digestive tract. International Journal of Systematic and Evolutionary Microbiology, 59, 2020-2024. DOI URL
[15]	Kim EK, Kim SH, Nam HJ, Choi MK, Lee KA, Choi SH, Seo YY, You H, Kim B, Lee WJ ( 2012) Draft genome sequence of Commensalibacter intestini A911T, a symbiotic bacterium isolated from Drosophila melanogaster intestine. Journal of Bacteriology, 194, 1246. DOI URL
[16]	Klein AM, Vaissière BE, Cane JH, Steffan-Dewenter I, Cunningham SA, Kremen C, Tscharntke T ( 2007) Importance of pollinators in changing landscapes for world crops. Proceedings of the Royal Society B: Biological Sciences, 274, 303-313. DOI URL
[17]	Kwong WK, Engel P, Koch H, Moran NA ( 2014 a) Genomics and host specialization of honey bee and bumble bee gut symbionts. Proceedings of the National Academy of Sciences, USA, 111, 11509-11514. DOI URL
[18]	Kwong WK, Mancenido AL, Moran NA ( 2014 b) Genome sequences of Lactobacillus sp. strains wkB8 and wkB10, members of the Firm-5 clade, from honey bee guts. Genome Announcements, 2, e01176-14.
[19]	Kwong WK, Medina LA, Koch H, Sing KW, Soh EJY, Ascher JS, Jaffé R, Moran NA ( 2017) Dynamic microbiome evolution in social bees. Science Advances, 3, 1-16.
[20]	Kwong WK, Moran NA ( 2013) Cultivation and characterization of the gut symbionts of honey bees and bumble bees: Description of Snodgrassella alvi gen. nov., sp. nov., a member of the family Neisseriaceae of the betaproteobacteria, and Gilliamella apicola gen. nov., sp. nov., a member of Orbaceae fam. nov., Orbales ord. nov., a sister taxon to the order 'Enterobacteriales' of the Gammaproteobacteria. International Journal of Systematic and Evolutionary Microbiology, 63, 2008-2018. DOI URL
[21]	Kwong WK, Moran NA ( 2016) Apibacter adventoris gen. nov., sp. nov., a member of the phylum Bacteroidetes isolated from honey bees. International Journal of Systematic and Evolutionary Microbiology, 66, 1323-1329. DOI URL
[22]	Lane DJ ( 1991) 16S/23S rRNA Sequencing. In: Nucleic Acid Techniques in Bacterial Systematic (eds Stackebrandt E, Goodfellow M), pp. 115-175. John Wiley and Sons, New York
[23]	Li L, Praet J, Borremans W, Nunes OC, Manaia CM, Cleenwerck I, Meeus I, Smagghe G, De Vuyst L, Vandamme P ( 2015) Bombella intestini gen. nov., sp. nov., an acetic acid bacterium isolated from bumble bee crop. International Journal of Systematic and Evolutionary Microbiology, 65, 267-273. DOI URL
[24]	Martinson VG, Danforth BN, Minckley RL, Rueppell O, Tingek S, Moran NA ( 2011) A simple and distinctive microbiota associated with honey bees and bumble bees. Molecular Ecology, 20, 619-628. DOI URL
[25]	Martinson VG, Mago T, Koch H, Salzberg SL, Moran NA ( 2014) Genomic features of a bumble bee symbiont reflect its host environment. Applied and Environmental Microbiology, 80, 3793-3803. DOI URL
[26]	Martinson VG, Moy J, Moran NA ( 2012) Establishment of characteristic gut bacteria during development of the honeybee worker. Applied and Environmental Microbiology, 78, 2830-2840. DOI URL
[27]	Mikaelyan A, Köhler T, Lampert N, Rohland J, Boga H, Meuser K, Brune A ( 2015) Classifying the bacterial gut microbiota of termites and cockroaches: A curated phylogenetic reference database (DictDb). Systematic and Applied Microbiology, 38, 472-482. DOI URL
[28]	Moran NA ( 2015) Genomics of the honey bee microbiome. Current Opinion in Insect Science, 10, 22-28. DOI URL
[29]	Newton IL, Roeselers G ( 2012) The effect of training set on the classification of honey bee gut microbiota using the Naïve Bayesian Classifier. BMC Microbiology, 12, 221. DOI URL
[30]	Olofsson TC, Alsterfjord M, Nilson B, Butler E, Vásquez A ( 2014) Lactobacillus apinorum sp. nov., Lactobacillus mellifer sp. nov., Lactobacillus mellis sp. nov., Lactobacillus melliventris sp. nov., Lactobacillus kimbladii sp. nov., Lactobacillus helsingborgensis sp. nov. and Lactobacillus kullabergensis sp. nov., isolated from the honey stomach of the honeybee Apis mellifera. International Journal of Systematic and Evolutionary Microbiology, 64, 3109-3119. DOI URL
[31]	Praet J, Aerts M, Brandt ED, Meeus I, Smagghe G, Vandamme P ( 2016) Apibacter mensalis sp. nov.: A rare member of the bumblebee gut microbiota. International Journal of Systematic and Evolutionary Microbiology, 66, 1645-1651. DOI URL
[32]	Praet J, Meeus I, Cnockaert M, Aerts M, Smagghe G, Vandamme P ( 2015) Bifidobacterium commune sp. nov. isolated from the bumble bee gut. Antonie van Leeuwenhoek, 107, 1307-1313. DOI URL
[33]	Praet J, Parmentier A, Schmid-Hempel R, Meeus I, Smagghe G, Vandamme P ( 2018) Large-scale cultivation of the bumblebee gut microbiota reveals an underestimated bacterial species diversity capable of pathogen inhibition. Environmental Microbiology, 20, 214-227. DOI URL
[34]	Pruesse E, Quast C, Knittel K, Fuchs BM, Ludwig W, Peplies J, Glöckner FO ( 2007) SILVA: A comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with ARB. Nucleic Acids Research, 35, 7188-7196. DOI URL
[35]	Raymann K, Shaffer Z, Moran NA ( 2017) Antibiotic exposure perturbs the gut microbiota and elevates mortality in honeybees. PLoS Biology, 15, e2001861. DOI URL
[36]	Segers FH, Kešnerová L, Kosoy M, Engel P ( 2017) Genomic changes associated with the evolutionary transition of an insect gut symbiont into a blood-borne pathogen. The ISME Journal, 11, 1232-1244. DOI
[37]	Steele MI, Kwong WK, Whiteley M, Moranb NA ( 2017) Diversification of type VI secretion system toxins reveals ancient antagonism among bee gut microbes. mBio, 8, e01630-17.
[38]	Tarpy DR, Mattila HR, Newton ILG ( 2015) Development of the honey bee gut microbiome throughout the Queen- Rearing Process. Applied and Environmental Microbiology, 81, 3182-3191. DOI URL
[39]	vanEngelsdorp D, Evans JD, Saegerman C, Mullin C, Haubruge E, Nguyen BK, Frazier M, Frazier J, Cox-Foster D, Chen Y, Underwood R, Tarpy DR, Pettis JS ( 2009) Colony collapse disorder: A descriptive study. PLoS ONE, 4, e6481. DOI URL
[40]	Wang Q, Garrity GM, Tiedje JM, Cole JR ( 2007) Naïve Bayesian Classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy. Applied and Environmental Microbiology, 73, 5261-5267. DOI URL
[41]	Yilmaz P, Parfrey LW, Yarza P, Gerken J, Pruesse E, Quast C, Schweer T, Peplies J, Ludwig W, Glöckner FO ( 2014) The SILVA and “All-species Living Tree Project (LTP)” taxonomic frameworks. Nucleic Acids Research, 42, D643-D648. DOI URL
[42]	Yun JH, Lee JY, Hyun DW, Jung MJ, Bae JW ( 2017) Bombella apis sp. nov., an acetic acid bacterium isolated from the midgut of a honey bee. International Journal of Systematic and Evolutionary Microbiology, 67, 2184-2188. DOI URL
[43]	Zheng H, Nishida A, Kwong WK, Koch H, Engel P, Steele MI, Moran NA ( 2016) Metabolism of toxic sugars by strains of the bee gut symbiont Gilliamella apicola. mBio, 7, e01326-16.
[44]	Zheng H, Powell JE, Steele MI, Dietrich C, Moran NA ( 2017) Honeybee gut microbiota promotes host weight gain via bacterial metabolism and hormonal signaling. Proceedings of the National Academy of Sciences, USA, 114, 4775-4780. DOI URL

属名 Genus	种名 Species	其他名 Other names	BGM-Db数据库聚类名 BGM-Db names	参考文献 References
Snodgrassella	Snodgrassella alvi	Beta	Snodgrassella	Martinson et al, 2012; Kwong & Moran, 2013
Gilliamella	Gilliamella apicola	Gamma-1	Gilliamella	Martinson et al, 2012; Kwong & Moran, 2013
Frischella	Frischella perrara	Gamma-2	Frischella	Engel et al, 2013
Schmidhempelia	‘Candidatus Schmidhempelia bombi’		Schmidhempelia	Martinson et al, 2014
Bartonella	Bartonella apis	Alpha-1	Bartonella apis	Moran, 2015
Commensalibacter	Commensalibacter intestini	Alpha-2.1	Commensalibacter Alpha2.1	Kwong et al, 2014b
Bombella	Bombella apis	Alpha-2.2	Bombella Alpha2.2	Yun et al, 2017
Bombella	Bombella intestini			Yun et al, 2017
Parasaccharibacter	Parasaccharibacter apium			Moran, 2015
Lactobacillus	Lactobacillus mellis	Firm-4	Lactobacillus Firm-4	Olofsson et al, 2014; Moran, 2015
	Lactobacillus mellifer	Firm-4	Lactobacillus Firm-4	Olofsson et al, 2014; Moran, 2015
	Lactobacillus apis	Firm-5	Lactobacillus Firm-5	Kwong et al, 2014b
	Lactobacillus helsingborgensis			Olofsson et al, 2014; Moran, 2015
	Lactobacillus melliventris
	Lactobacillus kimbladii
	Lactobacillus kullabergensis
	Lactobacillus apinorum
	Lactobacillus kunkeei	/	Lactobacillus kunkeei	Moran, 2015
Bifidobacterium	Bifidobacterium asteroides	/	Bifidobacterium asteroides	Bottacini et al, 2012
	Bifidobacterium coryneforme	/	Bifidobacterium coryneforme/indicum	Ellegaard et al, 2015
	Bifidobacterium indicum	/	Bifidobacterium coryneforme/indicum	Ellegaard et al, 2015
	Bifidobacterium bombi	/	Bifidobacterium bombi/commune/bohemicum	Killer et al, 2009
	Bifidobacterium commune	/		Praet et al, 2015
	Bifidobacterium bohemicum	/		Killer et al, 2011
Bombiscardovia	Bombiscardovia coagulans	/	Bombiscardovia	Killer et al, 2010
Apibacter	Apibacter adventoris	/	Apibacter	Kwong & Moran, 2016
	Apibacter mensalis	/	Apibacter	Praet et al, 2016

属名 Genus	种名 Species	其他名 Other names	BGM-Db数据库聚类名 BGM-Db names	参考文献 References
Snodgrassella	Snodgrassella alvi	Beta	Snodgrassella	Martinson et al, 2012; Kwong & Moran, 2013
Gilliamella	Gilliamella apicola	Gamma-1	Gilliamella	Martinson et al, 2012; Kwong & Moran, 2013
Frischella	Frischella perrara	Gamma-2	Frischella	Engel et al, 2013
Schmidhempelia	‘Candidatus Schmidhempelia bombi’		Schmidhempelia	Martinson et al, 2014
Bartonella	Bartonella apis	Alpha-1	Bartonella apis	Moran, 2015
Commensalibacter	Commensalibacter intestini	Alpha-2.1	Commensalibacter Alpha2.1	Kwong et al, 2014b
Bombella	Bombella apis	Alpha-2.2	Bombella Alpha2.2	Yun et al, 2017
Bombella	Bombella intestini			Yun et al, 2017
Parasaccharibacter	Parasaccharibacter apium			Moran, 2015
Lactobacillus	Lactobacillus mellis	Firm-4	Lactobacillus Firm-4	Olofsson et al, 2014; Moran, 2015
	Lactobacillus mellifer	Firm-4	Lactobacillus Firm-4	Olofsson et al, 2014; Moran, 2015
	Lactobacillus apis	Firm-5	Lactobacillus Firm-5	Kwong et al, 2014b
	Lactobacillus helsingborgensis			Olofsson et al, 2014; Moran, 2015
	Lactobacillus melliventris
	Lactobacillus kimbladii
	Lactobacillus kullabergensis
	Lactobacillus apinorum
	Lactobacillus kunkeei	/	Lactobacillus kunkeei	Moran, 2015
Bifidobacterium	Bifidobacterium asteroides	/	Bifidobacterium asteroides	Bottacini et al, 2012
	Bifidobacterium coryneforme	/	Bifidobacterium coryneforme/indicum	Ellegaard et al, 2015
	Bifidobacterium indicum	/	Bifidobacterium coryneforme/indicum	Ellegaard et al, 2015
	Bifidobacterium bombi	/	Bifidobacterium bombi/commune/bohemicum	Killer et al, 2009
	Bifidobacterium commune	/		Praet et al, 2015
	Bifidobacterium bohemicum	/		Killer et al, 2011
Bombiscardovia	Bombiscardovia coagulans	/	Bombiscardovia	Killer et al, 2010
Apibacter	Apibacter adventoris	/	Apibacter	Kwong & Moran, 2016
	Apibacter mensalis	/	Apibacter	Praet et al, 2016

用于蜜蜂和熊蜂肠道微生物分类的细菌16S rRNA数据库优化

A curated 16S rRNA reference database for the classification of honeybee and bumblebee gut microbiota

RichHTML

PDF (PC)

可视化

被引次数

摘要/Abstract

引用本文

使用本文

图/表 3

参考文献 44

相关文章 15

编辑推荐

Metrics

本文评价

[1]	夏凡, 杨婧, 李建, 史洋, 盖立新, 黄文华, 张经纬, 杨南, 高福利, 韩莹莹, 鲍伟东. 北京地区四个豹猫亚种群肠道菌群的组成[J]. 生物多样性, 2022, 30(9): 22103-.
[2]	孙翌昕, 李英滨, 李玉辉, 李冰, 杜晓芳, 李琪. 高通量测序技术在线虫多样性研究中的应用[J]. 生物多样性, 2022, 30(12): 22266-.
[3]	高程, 郭良栋. 微生物物种多样性、群落构建与功能性状研究进展[J]. 生物多样性, 2022, 30(10): 22429-.
[4]	夏呈强, 李毅, 党延茹, 察倩倩, 贺晓艳, 秦启龙. 中印度洋与南海西部表层海水细菌多样性[J]. 生物多样性, 2022, 30(1): 21407-.
[5]	陆奇丰, 黄至欢, 骆文华. 极小种群濒危植物广西火桐、丹霞梧桐的叶绿体基因组特征[J]. 生物多样性, 2021, 29(5): 586-595.
[6]	王楠, 黄菁华, 霍娜, 杨盼盼, 张欣玥, 赵世伟. 宁南山区不同植被恢复方式下土壤线虫群落特征:形态学鉴定与高通量测序法比较[J]. 生物多样性, 2021, 29(11): 1513-1529.
[7]	万雅琼, 李佳琦, 杨兴文, 李晟, 徐海根. 基于红外相机的中国哺乳动物多样性观测网络建设进展[J]. 生物多样性, 2020, 28(9): 1115-1124.
[8]	刘雪华, 张语克, 赵翔宇, 何祥博, 蔡琼, 朱云, 何百锁, 酒强. 秦岭中段野生动物多样性的红外相机监测数据库平台介绍[J]. 生物多样性, 2020, 28(9): 1075-1080.
[9]	靳新影, 张肖冲, 金多, 陈韵, 李靖宇. 腾格里沙漠东南缘不同生物土壤结皮细菌多样性及其季节动态特征[J]. 生物多样性, 2020, 28(6): 718-726.
[10]	韩本凤, 周欣, 张雪. 基因组学技术在病毒鉴定与宿主溯源中的应用[J]. 生物多样性, 2020, 28(5): 587-595.
[11]	张全建, 杨彪, 付强, 王磊, 龚旭, 张远彬. 邛崃山系水鹿的冬季食性[J]. 生物多样性, 2020, 28(10): 1192-1201.
[12]	王科,赵明君,苏锦河,杨柳,邓红,王永会,吴海军,李熠,吴红梅,卫晓丹,魏铁铮,蔡磊,姚一建. 中国菌物名录数据库在大型真菌红色名录编制中的作用[J]. 生物多样性, 2020, 28(1): 74-98.
[13]	陆琪,胡强,施小刚,金森龙,李晟,姚蒙. 基于分子宏条形码分析四川卧龙国家级自然保护区雪豹的食性[J]. 生物多样性, 2019, 27(9): 960-969.
[14]	刘君, 王宁, 崔岱宗, 卢磊, 赵敏. 小兴安岭大亮子河国家森林公园不同生境下土壤细菌多样性和群落结构[J]. 生物多样性, 2019, 27(8): 911-918.
[15]	刘君, 王宁, 崔岱宗, 卢磊, 赵敏. 大小兴安岭可培养细菌的资源多样性[J]. 生物多样性, 2019, 27(8): 903-910.