荒漠生物结皮细鞘丝藻类(Leptolyngbya-like)蓝藻多样性

doi:10.17520/biods.2024186

生物多样性 ›› 2024, Vol. 32 ›› Issue (9): 24186. DOI: 10.17520/biods.2024186

荒漠生物结皮细鞘丝藻类(Leptolyngbya-like)蓝藻多样性

陈静¹, 张丙昌¹^,^*(), 刘燕晋¹, 武杰¹, 赵康², 明姣¹

1.山西师范大学地理科学学院黄河中游生态环境研究中心, 太原 030031
2.山西师范大学生命科学学院, 太原 030031

收稿日期:2024-05-16 接受日期:2024-08-14 出版日期:2024-09-20 发布日期:2024-11-19
通讯作者: * E-mail: zhangbch@sxnu.edu.cn
基金资助:
国家自然科学基金(U2003214);国家自然科学基金(42271067)

Diversity of Leptolyngbya-like cyanobacteria in biocrusts in desert area

Jing Chen¹, Bingchang Zhang¹^,^*(), Yanjin Liu¹, Jie Wu¹, Kang Zhao², Jiao Ming¹

1. Research Center of Ecological Environment of Middle Reaches of Yellow River, Geography Science College, Shanxi Normal University, Taiyuan 030031, China
2. School of Life Sciences, Shanxi Normal University, Taiyuan 030031, China

Received:2024-05-16 Accepted:2024-08-14 Online:2024-09-20 Published:2024-11-19
Contact: * E-mail: zhangbch@sxnu.edu.cn
Supported by:
National Natural Science Foundation of China(U2003214);National Natural Science Foundation of China(42271067)

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摘要/Abstract

摘要：

细鞘丝藻类蓝藻(Leptolyngbya-like cyanobacteria)是干旱生态系统生物结皮中的常见藻种, 其形态特征差别细微, 仅依靠形态特征难以在物种水平对其进行准确分类。本文利用形态特征、16S rRNA谱系分析和ITS (internal transcribed spacer)二级结构相结合的多相分析方法, 系统研究了古尔班通古特沙漠和毛乌素沙地生物土壤结皮中分离纯化出的6株细丝状蓝藻。结果表明, 6株实验藻株隶属2目3科4属4种, 包括细鞘丝藻目细鞘丝藻科细鞘丝藻属(Leptolyngbya)的鲍氏细鞘丝藻(L. boryana)、粘鞘藻属(Myxacorys)的加利福尼亚粘鞘藻(M. californica)和须鞘藻科须鞘藻属(Trichocoleus)的沙生须鞘藻(T. desertorum), 以及细点丝藻目细点丝藻科细点丝藻属(Oculatella)的细长细点丝藻(O. coburniia)。其中细点丝藻目为中国新记录目, 须鞘藻科和细点丝藻科为中国新记录科, 粘鞘藻属、须鞘藻属和细点丝藻属为中国新记录属; 沙生须鞘藻、加利福尼亚粘鞘藻和细长细点丝藻是中国新记录种。该研究结果丰富了对荒漠生物结皮蓝藻多样性的认识。

关键词: 生物结皮, 细鞘丝藻类蓝藻, 形态特征, 16S rRNA, ITS二级结构

Abstract

Aims: Biological soil crusts are an important landscape in arid and semi-arid ecosystems. Leptolyngbya-like cyanobacteria are common groups of cyanobacteria found in biocrusts within arid ecosystem. However, their morphological characteristics are often subtle, making species-level differentiation based solely on morphological characteristics difficult. This study aims to enrich the understanding of the species diversity of biological crust cyanobacteria in arid and semi-arid regions by integrating morphological characteristics with molecular biology.
Method: In this study, six strains of Leptolyngbya-like cyanobacteria, isolated from the Gurbantunggut Desert and the Mu Us Desert, were identified by morphological characteristics, 16S rRNA molecular sequences, and ITS (internal transcribed spacer) secondary structure methods.
Results: The results showed that all the experimental cyanobacterial strains belonged to 4 species and 4 genus of 3 families, 2 orders, including Leptolyngbya boryana of Leptolyngbya and Myxacorys californica of Myxacorys in Leptolyngbyaceae, Trichocoleus desertorum of Trichocoleus in Trichocoleusaceae of Leptolynabgales, and Oculatella coburniia of Oculatella in Oculatellaceae of Oculatellales.
Conclusion: We found Oculatellales as a new record order in China, along with Trichocoleusaceae and Oculatellaceae as new record families, Myxacorys, Trichocoleus, and Oculatella as new record genera. Additionally, T. desertorum, M. californica, and O. coburniia are newly recorded species in China. These findings enhance our understanding of cyanobacterial diversity in biocrusts in desert areas.

Key words: biocrusts, Leptolyngbya-like cyanobacteria, morphological characteristic, 16S rRNA, ITS second structure

陈静, 张丙昌, 刘燕晋, 武杰, 赵康, 明姣 (2024) 荒漠生物结皮细鞘丝藻类(Leptolyngbya-like)蓝藻多样性. 生物多样性, 32, 24186. DOI: 10.17520/biods.2024186.

Jing Chen, Bingchang Zhang, Yanjin Liu, Jie Wu, Kang Zhao, Jiao Ming (2024) Diversity of Leptolyngbya-like cyanobacteria in biocrusts in desert area. Biodiversity Science, 32, 24186. DOI: 10.17520/biods.2024186.

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链接本文: https://www.biodiversity-science.net/CN/10.17520/biods.2024186

https://www.biodiversity-science.net/CN/Y2024/V32/I9/24186

图/表 6

图1 荒漠带细鞘丝藻类蓝藻藻株形态(箭头所指方向为藻株粘液帽)。1: CXA030; 2: CXA033; 3: CXA025; 4: 93-2-1; 5: 124-3-1; 6: 1345-1-1。样品CXA030、CXA033、CXA025、93-2-1、124-3-1来自古尔班通古特沙漠, 1345-1-1来自毛乌素沙地。

Fig. 1 Morphology of Leptolyngbya-like cyanobacteria strains in the desert zone (The direction of the arrow is the algae stalk mucus cap). 1, CXA030; 2, CXA033; 3, CXA025; 4, 93-2-1; 5, 124-3-1; 6, 1345-1-1. Samples of CXA030, CXA033, CXA025, 93-2-1, and 124-3-1 are from the Gurbantunggut Desert, while sample 1345-1-1 is from the Mu Us Desert.

图2 荒漠带细鞘丝藻类蓝藻藻株基于16S rRNA基因的系统进化树。(a)最大似然法系统进化树; (b)贝叶斯系统进化树。*加粗表示实验藻株。

Fig. 2 Phylogenetic tree of Leptolyngbya-like cyanobacteria strains based on the 16S rRNA gene in the desert zone. (a) Phylogenetic tree based on maximum likelihood method; (b) Phylogenetic tree based on Bayesian inference method. *

表1 本研究藻株的16S-23S rRNA内部转录间隔区(ITS)分析

Table 1 Analyses of 16S-23S rRNA ITS region of the strains in this study

藻株 Strain	ITS全长 Complete ITS (nt)	D1-D1'螺旋 D1-D1' Helix (nt)	异亮氨酸转运RNA tRNA^Ile (nt)	丙氨酸转运RNA tRNA^Ala (nt)	Box-B螺旋 Box-B Helix (nt)	V3螺旋 V3 Helix (nt)
CXA030	332	57	-	-	33	89
CXA033	332	57	-	-	33	89
CXA025	519	62	74	73	37	36
93-2-1	443	64	-	-	33	51
124-3-1	435	64	-	-	33	46
1345-1-1	519	64	74	73	35	52

图3 荒漠带细鞘丝藻类蓝藻的D1-D1′螺旋结构

Fig. 3 D1-D1′ helix structure of Leptolyngbya-like cyanobacteria in the desert zone. a, CXA030 and CXA033; b, Leptolyngbya boryana (OQ453216); c, L. corticola (EF429300); d, CXA025; e, Trichocoleus desertorum (KF307614)/T. badius (EF429297); f, 93-2-1/124-3-1; g, Myxacorys californica (KJ939052); h, M. chilensis (KJ939079); i, 1345-1-1; j, Oculatella coburnii (AY239600); k, O. mojaviensis (KF761572).

图4 荒漠带细鞘丝藻类蓝藻的BOX-B螺旋结构

Fig. 4 BOX-B helix structure of Leptolyngbya-like cyanobacteria in the desert zone. a, CXA030 and CXA033; b, Leptolyngbya boryana (OQ453216); c, L. corticola (EF429300); d, CXA025; e, Trichocoleus desertorum (KF307614); f, T. badius (EF429297); g, 93-2-1; h, 124-3-1; i, Myxacorys californica (KJ939052); j, M. chilensis (KJ939079); k, 1345-1-1; l, Oculatella coburnii (AY239600); M, O. mojaviensis (KF761572).

图5 荒漠带细鞘丝藻类蓝藻的V3螺旋结构

Fig. 5 V3 helix structure of Leptolyngbya-like cyanobacteria in the desert zone. a, CXA030; b, CXA033; c, Leptolyngbya boryana (OQ453216); d, L. corticola (EF429300); e, CXA025; f, Trichocoleus desertorum (KF307614); g, T. badius (EF429297); h, 93-2-1; i, 124-3-1; j, Myxacorys californica (KJ939052); k, M. chilensis (KJ939079); l, 1345-1-1; m, Oculatella coburnii (AY239600); n, O. mojaviensis (KF761572).

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荒漠生物结皮细鞘丝藻类(Leptolyngbya-like)蓝藻多样性

Diversity of Leptolyngbya-like cyanobacteria in biocrusts in desert area

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