生物多样性 ›› 2013, Vol. 21 ›› Issue (2): 197-205.DOI: 10.3724/SP.J.1003.2013.09160

• 研究报告 • 上一篇    下一篇

DNA条形码技术在小型兽类鉴定中的探索: 以甘肃莲花山为例

何锴1, 王文智1, 李权1,2, 罗培鹏3, 孙悦华4, 蒋学龙1*   

  1. 1中国科学院昆明动物研究所遗传资源与进化国家重点实验室, 昆明 650223
    2中国科学院大学, 北京 100049
    3莲花山国家级自然保护区管理局, 甘肃康乐 731516
    4中国科学院动物研究所动物进化与系统学重点实验室, 北京 100101
  • 收稿日期:2012-08-14 修回日期:2013-01-18 出版日期:2013-03-20 发布日期:2013-04-02
  • 通讯作者: 蒋学龙
  • 基金资助:

    基金项目:中科院昆明动物所遗传资源与进化国家重点实验室开放课题基金;中国科学院知识创新工程重要方向项目

DNA barcoding in surveys of small mammal community: a case study in Lianhuashan, Gansu Province, China

Kai He1, Wenzhi Wang1, Quan Li1,2, Peipeng Luo3, Yuehua Sun4, Xuelong Jiang1*   

  1. 1State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223

    2University of Chinese Academy of Sciences, Beijing 100049

    3Administrative Bureau of Lianhuashan National Nature Reserve, Kangle, Gansu 731516

    4Key Laboratory of Animal Ecology and Conservation Biology, Chinese Academy of Sciences, Beijing 100101
  • Received:2012-08-14 Revised:2013-01-18 Online:2013-03-20 Published:2013-04-02
  • Contact: Xuelong Jiang

摘要:

为验证DNA条形码技术在小型兽类物种鉴定中的可行性, 我们使用传统的形态学方法和DNA条形码技术对甘肃莲花山国家级自然保护区采集的77号小型兽类标本进行鉴定。共鉴定出物种13种, 分别属于3目7科。其中小纹背鼩鼱(Sorex bedfordiae)、藏鼩鼱(S. thibetanus)、甘肃鼹(Scapanulus oweni)、黑线姬鼠(Apodemus agrarius)、社鼠(Niviventer confucianus)、斯氏鼢鼠(Eospalax smithi)、高原鼠兔(Ochotona curzoniae)等7种为莲花山首次报道。另外, 采自海拔2,800 m的黑线姬鼠是该物种全球海拔最高的分布记录。通过对两种方法进行比较, 我们发现形态鉴定需要研究者具有足够的分类学背景, 基于DNA条形码的物种鉴定则限制于网络数据库中缺乏足够的序列信息导致成功率不高。因此我们认为现阶段形态鉴定依然是小型兽类鉴定的基石和主要手段, DNA条形码技术可以作为重要的补充手段, 对形态鉴定的结果进行检验。最后本文认为DNA条形码鉴定前景非常乐观, 但在现阶段的使用中应与经典分类紧密结合并尽可能保存凭证标本。

Abstract:

To evaluate the efficiency of DNA barcoding for identifying small mammals, and to enhance current understanding of mammalian diversity in the Lianhuashan Mountains, Gansu, China, standard morphological and DNA barcoding approaches were used to identify 77 small mammal specimens sampled from the Lianhuashan National Nature Reserve, Gansu. Thirteen species identified were assigned to seven families in three orders, among which seven species (Sorex bedfordiae, S. thibetanus, Scapanulus oweni, Apodemus agrarius, Niviventer confucianus, Eospalax smithi and Ochotona curzoniae) are new records for the Lianhuashan Mountains. Apodemus agrarius collected at 2,800 m above sea level is the highest altitude recorded for this species. We compared the success rate and accuracy of morphological and DNA barcoding approaches for identification. We found that morphological diagnosis requires well-preserved specimens and expert taxonomists, while success rate and accuracy of barcoding-based identification was limited by insufficient sequences in the genetic database. We suggest that morphology should still be the foundation and principal approach used for small mammal identification. Currently, DNA barcoding might be used as a complement for checking and correcting morphological mis-diagnosis.