基于SRAP分子标记的海南沼虾种群遗传多样性

doi:10.3724/SP.J.1003.2010.150

生物多样性 ›› 2010, Vol. 18 ›› Issue (2): 145-149. DOI: 10.3724/SP.J.1003.2010.150

基于SRAP分子标记的海南沼虾种群遗传多样性

傅洪拓¹^,²^,^*(), 乔慧², 姚建华², 龚永生¹, 吴滟¹, 蒋速飞¹, 熊贻伟¹

¹中国水产科学研究院淡水渔业研究中心, 农业部水生动物遗传育种和养殖生物学重点开放实验室, 江苏无锡 214081
²南京农业大学无锡渔业学院, 江苏无锡 214081

收稿日期:2009-07-07 接受日期:2010-01-01 出版日期:2010-03-20 发布日期:2010-03-20
通讯作者: 傅洪拓
基金资助:
国家“十一五”科技支撑计划(2006BAD01A13);农业部农业科技跨越计划(2007年度);江苏省高技术研究项目(BG2007328)

Genetic diversity in five Macrobrachium hainanense populations using SRAP markers

Hongtuo Fu¹^,²^,^*(), Hui Qiao², Jianhua Yao², Yongsheng Gong¹, Yan Wu¹, Sufei Jiang¹, Yiwei Xiong¹

¹Freshwater Fishery Research Center of Chinese Academy of Fishery Sciences, Key Open Laboratory for Genetic Breeding of Aquatic Animals and Aquaculture Biology, Ministry of Agriculture, Wuxi, Jiangsu 214081
²Wuxi Fishery College, Nanjing Agricultural University, Wuxi, Jiangsu 214081

Received:2009-07-07 Accepted:2010-01-01 Online:2010-03-20 Published:2010-03-20
Contact: Hongtuo Fu

摘要/Abstract

摘要：

为了调查我国海南沼虾(Macrobrachium hainanense)不同地理种群遗传多样性及遗传分化状况, 本文采用序列相关扩增多态性(sequence-related amplified polymorphism, SRAP)标记对我国南方瓯江(OJ)、闽江(MJ)、珠江(PR)、万泉河(WQ)、昌化江(CH)等5个海南沼虾种群的遗传多样性进行了研究。共得到255个清晰、稳定的位点, 平均多态位点比例为47.05%, 由小到大依次为: PR (43.92%) = WQ (43.92%) < MJ (46.67%) < CH (47.45%) < OJ (53.33%)。遗传杂合度由小到大依次为: PR (0.1657) < WQ (0.1763) < CH (0.1799) < OJ (0.1839) < MJ (0.1892), 平均为0.1790。Shannon信息指数由小到大依次为: PR (0.2543) < WQ (0.2658) < CH (0.2746) < MJ (0.2846) < OJ (0.2876), 平均为0.2734。AMOVA分析结果显示, 17.64%的遗传变异来自种群间, 对总遗传变异影响显著( P<0.001)。遗传分化指数(G_st)、基因流(N_m)、遗传距离和聚类分析结果均显示5个海南沼虾种群间已经出现较大的遗传分化。

关键词: Macrobrachium hainanense, SRAP, 遗传多样性, 遗传分化, 种群遗传结构

Abstract

We studied the genetic variation and differentiation of five wild populations of Macrobrachium hainanense using SRAP markers. Samples were collected from Oujiang River (OJ), Minjiang River (MJ), Pearl River (PR), Wanquan River (WQ) and Changhuajiang River (CH). A total of 255 loci were detected, of which 47.05% were polymorphic. Proportion of polymorphic loci of the five populations ranked in the order: PR (43.92%) = WQ (43.92%) < MJ (46.67%) < CH (47.45%) < OJ (53.33%), while heterozygosity ranked as PR (0.1657) < WQ (0. 1763) < CH (0. 179) < OJ (0. 1839) < MJ (0. 1892), with an average of 0.1790. Shannon index ranked as PR (0.2543) < WQ (0.2658) < CH (0.2746) < MJ (0.2846) < OJ (0.2876), with an average of 0.2734. Analysis of molecular variation (AMOVA) revealed that 17.64% of the variance was among populations, which significantly affected the total variance ( P <0.001). Values of G_st, N_m and genetic distance suggested the genetic divergence among populations was relatively high (P < 0.001).

Key words: Macrobrachium hainanense, SRAP, genetic diversity, genetic differentiation, population structure

傅洪拓, 乔慧, 姚建华, 龚永生, 吴滟, 蒋速飞, 熊贻伟 (2010) 基于SRAP分子标记的海南沼虾种群遗传多样性. 生物多样性, 18, 145-149. DOI: 10.3724/SP.J.1003.2010.150.

Hongtuo Fu, Hui Qiao, Jianhua Yao, Yongsheng Gong, Yan Wu, Sufei Jiang, Yiwei Xiong (2010) Genetic diversity in five Macrobrachium hainanense populations using SRAP markers. Biodiversity Science, 18, 145-149. DOI: 10.3724/SP.J.1003.2010.150.

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链接本文: https://www.biodiversity-science.net/CN/10.3724/SP.J.1003.2010.150

https://www.biodiversity-science.net/CN/Y2010/V18/I2/145

图/表 8

参考文献 11

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种群代码 Population	时间 Time	采样地点 Location
瓯江 OJ	2008.10	瓯江双潮段 Shuangchao section, Oujiang River (120.65°E, 28.01°N)
闽江 MJ	2008.7	闽江福州段 Fuzhou section, Minjiang River (119.30°E, 26.08°N)
珠江 PR	2007.4	珠江广州段 Guangzhou section, Zhujiang River (113.23°E, 23.16°N)
昌化江 CH	2008.9.	昌化江叉河段 Chahe section, Changhua River (109.00°E, 19.20°N)
万泉河 WQ	2008.9.	万泉河万泉段 Wanquan section, Wan- quan River (110.46°E, 19.25°N)

种群代码 Population	时间 Time	采样地点 Location
瓯江 OJ	2008.10	瓯江双潮段 Shuangchao section, Oujiang River (120.65°E, 28.01°N)
闽江 MJ	2008.7	闽江福州段 Fuzhou section, Minjiang River (119.30°E, 26.08°N)
珠江 PR	2007.4	珠江广州段 Guangzhou section, Zhujiang River (113.23°E, 23.16°N)
昌化江 CH	2008.9.	昌化江叉河段 Chahe section, Changhua River (109.00°E, 19.20°N)
万泉河 WQ	2008.9.	万泉河万泉段 Wanquan section, Wan- quan River (110.46°E, 19.25°N)

上游引物 Forward primers		下游引物 Reverse primers
me1	5′-TGAGTCCAAACCGGATA-3′	em1	5′-GACTGCGTACGAATTAAT-3′
me2	5′-TGAGTCCAAACCGGAGC-3′	em2	5′-GACTGCGTACGAATTTGC-3′
me3	5′-TGAGTCCAAACCGGAAT-3′	em3	5′-GACTGCGTACGAATTGAC-3′
me4	5′-TGAGTCCAAACCGGACC-3′	em4	5′-GACTGCGTACGAATTTGA-3′
me5	5′-TGAGTCCAAACCGGAAG-3′	em5	5′-GACTGCGTACGAATTAAC-3′
		em6	5′-GACTGCGTACGAATTGCA-3′

上游引物 Forward primers		下游引物 Reverse primers
me1	5′-TGAGTCCAAACCGGATA-3′	em1	5′-GACTGCGTACGAATTAAT-3′
me2	5′-TGAGTCCAAACCGGAGC-3′	em2	5′-GACTGCGTACGAATTTGC-3′
me3	5′-TGAGTCCAAACCGGAAT-3′	em3	5′-GACTGCGTACGAATTGAC-3′
me4	5′-TGAGTCCAAACCGGACC-3′	em4	5′-GACTGCGTACGAATTTGA-3′
me5	5′-TGAGTCCAAACCGGAAG-3′	em5	5′-GACTGCGTACGAATTAAC-3′
		em6	5′-GACTGCGTACGAATTGCA-3′

引物组合Primer combination	位点总数Total fragments	引物组合 Primer combination	位点总数 Total fragments
me1-em1	15	me3-em6	13
me1-em3	14	me4-em2	13
me1-em6	24	me4-em3	19
me2-em2	16	me4-em6	22
me2-em3	12	me5-em1	19
me2-em5	14	me5-em2	23
me2-em6	21	me5-em4	20
me3-em2	10

基于SRAP分子标记的海南沼虾种群遗传多样性

Genetic diversity in five Macrobrachium hainanense populations using SRAP markers

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种群 Population	多态位点数 POL	多态位点比例 PPB(%)	遗传杂合度 H_E	Shannon信息指数 I
瓯江 OJ	136	53.33%	0.1839	0.2876
闽江 MJ	119	46.67%	0.1892	0.2846
珠江 PR	112	43.92%	0.1657	0.2543
昌化江 CH	121	47.45%	0.1799	0.2746
万泉河 WQ	112	43.92%	0.1763	0.2658

种群名称 Population	瓯江OJ	闽江MJ	珠江PR	昌化江CH	万泉河WQ
瓯江 OJ	****	3.6923	5.1366	2.7006	3.2665
闽江 MJ	0.1193	****	3.9067	1.9135	3.9127
珠江 PR	0.0887	0.1135	****	2.0981	3.1088
昌化江 CH	0.1526	0.2702	0.1924	****	2.0543
万泉河 WQ	0.1327	0.1133	0.1386	0.1958	****

变异来源 Source of variation	自由度 df	平方和 Sum of square	变异组分 Component of variation	变异贡献率 Contribution rate of variation	P
种群间 Among populations	4	257.8857	5.5245	17.64%^**	P<0.001
种群内 Within populations	32	774.0000	25.8000	82.36%^**	P<0.001
合计 Total	36	1,031.8857	31.3245

种群名称 Populations	瓯江OJ	闽江MJ	珠江PR	昌化江CH	万泉河WQ
瓯江 OJ	****	0.7355	0.7528	0.6718	0.7379
闽江 MJ	0.2645	****	0.7475	0.6531	0.7406
珠江 PR	0.2472	0.2525	****	0.6784	0.7446
昌化江 CH	0.3282	0.3469	0.3216	****	0.6711
万泉河 WQ	0.2621	0.2594	0.2554	0.3289	****

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