棉花耐盐相关种质资源遗传多样性分析

doi:10.3724/SP.J.1003.2010.142

摘要/Abstract

摘要：

为了解我国棉花耐盐相关种质资源的遗传变异, 利用88对SSR引物对23份棉花耐盐材料和24份盐敏感材料进行遗传多样性分析。88个SSR位点在47份材料中共检测出338个等位基因变异, 平均每个位点有3.841个; 其中耐盐材料中检测出333个, 盐敏感材料中检测出312个。耐盐材料的位点多态信息含量(PIC)、每个位点的有效等位基因数(Ne)、基因型多样性(H′)分别为0.613、2.929和1.083, 盐敏感材料的PIC、Ne、H′分别为0.605、2.883和1.071。耐盐材料和盐敏感材料的Jaccard相似性系数分别在0.530-0.979和0.525-0.878之间, 遗传相似性系数总体平均值接近, 但耐盐材料的变化幅度更大。用类平均法(UPGMA)聚类将47份材料分成3个类群。总体而言, 大多数材料之间的遗传相似性系数较高, 表明我国陆地棉耐盐相关种质资源遗传基础狭窄。本结果为棉花耐盐育种中亲本的选配和优势组合的预测以及耐盐资源的合理利用等提供了基础资料。

关键词: 陆地棉, 耐盐性, SSR, 遗传相似性, 耐盐育种

Abstract

In order to study the genetic variation of salinity related cotton germplasm, 47 upland cotton accessions including 23 salinity tolerant materials and 24 salinity sensitive materials were explored using 88 simple sequence repeat (SSR) markers. We detected a total of 338 alleles at 88 SSR loci with an average of 3.841 alleles per locus, 333 of these alleles were detected in salinity tolerant germplasm and 312 alleles in salinity sensitive germplasm. Mean polymorphism information content (PIC), the average effective numbers of alleles (Ne) and the average genotype diversity index (H′) were 0.613, 2.929 and 1.083 in salinity tolerant germplasm, and 0.605, 2.883 and 1.071 in salinity sensitive germplasm, respectively. The similarity coefficients were similar between salinity tolerant germplasm and salinity sensitive germplasm. They varied from 0.530 to 0.979 in salinity tolerant germplasm, with a wider range than in salinity tolerant germplasm (from 0.525 to 0.878). The varieties were clustered into one major group and two small groups. The high genetic similarity coefficients we observed in Chinese salinity tolerant germplasm indicated narrow pedigrees within the group. Our results are useful for assessing cotton pedigrees, improving cotton hybrids, and ultimately allowing for the improved utilization of salinity tolerant germplasm.

Key words: upland cotton, salinity tolerance, SSR, genetic similarity, breeding for salinity tolerance

张丽娜, 叶武威, 王俊娟, 樊保香, 王德龙 (2010) 棉花耐盐相关种质资源遗传多样性分析. 生物多样性, 18, 137-144. DOI: 10.3724/SP.J.1003.2010.142.

Lina Zhang, Wuwei Ye, Junjuan Wang, Baoxiang Fan, Delong Wang (2010) Genetic diversity analysis of salinity related germplasm in cotton. Biodiversity Science, 18, 137-144. DOI: 10.3724/SP.J.1003.2010.142.

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链接本文: https://www.biodiversity-science.net/CN/10.3724/SP.J.1003.2010.142

https://www.biodiversity-science.net/CN/Y2010/V18/I2/137

图/表 5

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编号 No.	名称 Name	产地或来源 Source or Origin	相对耐盐成活苗率 Salinity-resistance index (%)	耐盐级别 Level of salinity-tolerance
1	CT114	中棉所 CCRI	77.35	抗 Salt-resistance
2	CT115	中棉所 CCRI	76.15	抗 Salt-resistance
3	CT06	中棉所 CCRI	75.00	抗 Salt-resistance
4	CT05	中棉所 CCRI	75.80	抗 Salt-resistance
5	中07 Zhong07	中棉所 CCRI	58.37	耐 Salt-tolerance
6	中9806 Zhong9806	中棉所 CCRI	51.43	耐 Salt-tolerance
7	中9835 Zhong9835	中棉所 CCRI	54.94	耐 Salt-tolerance
8	CT02	中棉所 CCRI	65.47	耐 Salt-tolerance
9	CT 186	中棉所 CCRI	65.64	耐 Salt-tolerance
10	CT 5-10	中棉所 CCRI	61.86	耐 Salt-tolerance
11	CT 637	中棉所 CCRI	57.88	耐 Salt-tolerance
12	CT1-1	中棉所 CCRI	61.00	耐 Salt-tolerance
13	CT03	中棉所 CCRI	53.91	耐 Salt-tolerance
14	CT04	中棉所 CCRI	58.33	耐 Salt-tolerance
15	CT 924	中棉所 CCRI	55.41	耐 Salt-tolerance
16	CT01	中棉所 CCRI	54.21	耐 Salt-tolerance
17	中选41 Zhongxuan41	中棉所 CCRI	53.52	耐 Salt-tolerance
18	邯109 Han109	河北 Hebei	66.38	耐 Salt-tolerance
19	中植棉8号 Zhongzhimian8	河北 Hebei	67.50	耐 Salt-tolerance
20	豫2067 Yu2067	河南 Hebei	64.60	耐 Salt-tolerance
21	新陆早12号 Xinluzao12	新疆 Xinjiang	52.56	耐 Salt-tolerance
22	新陆早36号 Xinluzao36	新疆 Xinjiang	51.16	耐 Salt-tolerance
23	新陆早19 Xinluzao19	新疆 Xinjiang	55.09	耐 Salt-tolerance
24	抗黄萎164 Kanghuangwei164	中棉所 CCRI	33.92	不耐 Salt-sensitive
25	中S9612 ZhongS9612	中棉所 CCRI	41.60	不耐 Salt-sensitive
26	Cap500	中棉所 CCRI	34.15	不耐 Salt-sensitive
27	邯177 Han177	河北 Hebei	29.73	不耐 Salt-sensitive
28	丰抗棉1号 Fengkangmian1	河北 Hebei	45.30	不耐 Salt-sensitive
29	邯棉103 Hanmian103	河北 Hebei	37.22	不耐 Salt-sensitive
30	快丰868 Kuaifeng868	河北 Hebei	40.62	不耐 Salt-sensitive
31	冀丰106 Jifeng106	河北 Hebei	41.91	不耐 Salt-sensitive
32	邯5158 Han5158	河北 Hebei	36.57	不耐 Salt-sensitive
33	国欣棉11号 Guoxinmian11	河北 Hebei	45.00	不耐 Salt-sensitive
34	GK50	河北 Hebei	38.69	不耐 Salt-sensitive
35	新研96-48 Xinyan96-48	河南 Henan	34.91	不耐 Salt-sensitive
36	中棉所45 CCRI45	河南 Henan	49.13	不耐 Salt-sensitive
37	中棉所49 CCRI49	河南 Henan	48.89	不耐 Salt-sensitive
38	银山6号 Yinshan6	河南 Henan	45.00	不耐 Salt-sensitive
39	鑫秋1号Xinqiu1	山东 Shandong	40.32	不耐 Salt-sensitive
40	银瑞361 Yinrui361	山东 Shandong	36.03	不耐 Salt-sensitive
41	鲁棉研27 SCRC27	山东 Shandong	45.00	不耐 Salt-sensitive
42	鲁棉研21 SCRC21	山东 Shandong	40.62	不耐 Salt-sensitive
43	鲁棉研17 SCRC17	山东 Shandong	38.89	不耐 Salt-sensitive
44	鲁棉研16 SCRC16	山东 Shandong	46.12	不耐 Salt-sensitive
45	新疆-2 Xinjiang-2	新疆 Xinjiang	22.07	不耐 Salt-sensitive
46	新陆中26号 Xinluzhong26	新疆 Xinjiang	43.78	不耐 Salt-sensitive
47	新陆早33号 Xinluzao33	新疆 Xinjiang	46.00	不耐 Salt-sensitive

编号 No.	名称 Name	产地或来源 Source or Origin	相对耐盐成活苗率 Salinity-resistance index (%)	耐盐级别 Level of salinity-tolerance
1	CT114	中棉所 CCRI	77.35	抗 Salt-resistance
2	CT115	中棉所 CCRI	76.15	抗 Salt-resistance
3	CT06	中棉所 CCRI	75.00	抗 Salt-resistance
4	CT05	中棉所 CCRI	75.80	抗 Salt-resistance
5	中07 Zhong07	中棉所 CCRI	58.37	耐 Salt-tolerance
6	中9806 Zhong9806	中棉所 CCRI	51.43	耐 Salt-tolerance
7	中9835 Zhong9835	中棉所 CCRI	54.94	耐 Salt-tolerance
8	CT02	中棉所 CCRI	65.47	耐 Salt-tolerance
9	CT 186	中棉所 CCRI	65.64	耐 Salt-tolerance
10	CT 5-10	中棉所 CCRI	61.86	耐 Salt-tolerance
11	CT 637	中棉所 CCRI	57.88	耐 Salt-tolerance
12	CT1-1	中棉所 CCRI	61.00	耐 Salt-tolerance
13	CT03	中棉所 CCRI	53.91	耐 Salt-tolerance
14	CT04	中棉所 CCRI	58.33	耐 Salt-tolerance
15	CT 924	中棉所 CCRI	55.41	耐 Salt-tolerance
16	CT01	中棉所 CCRI	54.21	耐 Salt-tolerance
17	中选41 Zhongxuan41	中棉所 CCRI	53.52	耐 Salt-tolerance
18	邯109 Han109	河北 Hebei	66.38	耐 Salt-tolerance
19	中植棉8号 Zhongzhimian8	河北 Hebei	67.50	耐 Salt-tolerance
20	豫2067 Yu2067	河南 Hebei	64.60	耐 Salt-tolerance
21	新陆早12号 Xinluzao12	新疆 Xinjiang	52.56	耐 Salt-tolerance
22	新陆早36号 Xinluzao36	新疆 Xinjiang	51.16	耐 Salt-tolerance
23	新陆早19 Xinluzao19	新疆 Xinjiang	55.09	耐 Salt-tolerance
24	抗黄萎164 Kanghuangwei164	中棉所 CCRI	33.92	不耐 Salt-sensitive
25	中S9612 ZhongS9612	中棉所 CCRI	41.60	不耐 Salt-sensitive
26	Cap500	中棉所 CCRI	34.15	不耐 Salt-sensitive
27	邯177 Han177	河北 Hebei	29.73	不耐 Salt-sensitive
28	丰抗棉1号 Fengkangmian1	河北 Hebei	45.30	不耐 Salt-sensitive
29	邯棉103 Hanmian103	河北 Hebei	37.22	不耐 Salt-sensitive
30	快丰868 Kuaifeng868	河北 Hebei	40.62	不耐 Salt-sensitive
31	冀丰106 Jifeng106	河北 Hebei	41.91	不耐 Salt-sensitive
32	邯5158 Han5158	河北 Hebei	36.57	不耐 Salt-sensitive
33	国欣棉11号 Guoxinmian11	河北 Hebei	45.00	不耐 Salt-sensitive
34	GK50	河北 Hebei	38.69	不耐 Salt-sensitive
35	新研96-48 Xinyan96-48	河南 Henan	34.91	不耐 Salt-sensitive
36	中棉所45 CCRI45	河南 Henan	49.13	不耐 Salt-sensitive
37	中棉所49 CCRI49	河南 Henan	48.89	不耐 Salt-sensitive
38	银山6号 Yinshan6	河南 Henan	45.00	不耐 Salt-sensitive
39	鑫秋1号Xinqiu1	山东 Shandong	40.32	不耐 Salt-sensitive
40	银瑞361 Yinrui361	山东 Shandong	36.03	不耐 Salt-sensitive
41	鲁棉研27 SCRC27	山东 Shandong	45.00	不耐 Salt-sensitive
42	鲁棉研21 SCRC21	山东 Shandong	40.62	不耐 Salt-sensitive
43	鲁棉研17 SCRC17	山东 Shandong	38.89	不耐 Salt-sensitive
44	鲁棉研16 SCRC16	山东 Shandong	46.12	不耐 Salt-sensitive
45	新疆-2 Xinjiang-2	新疆 Xinjiang	22.07	不耐 Salt-sensitive
46	新陆中26号 Xinluzhong26	新疆 Xinjiang	43.78	不耐 Salt-sensitive
47	新陆早33号 Xinluzao33	新疆 Xinjiang	46.00	不耐 Salt-sensitive

引物 Primer	定位染色体 Chromosome	PIC	Ne	H'	引物 Primer	定位染色体 Chromosome	PIC	Ne	H'
Y12	24	0.626	2.672	1.030	Y172	-	0.653	2.885	1.107
Y272	13, 18	0.602	2.510	1.021	Y175	-	0.639	2.768	1.059
Y16	15	0.610	2.561	1.018	Y177	-	0.607	2.548	1.014
Y02	7, 13, 16	0.752	4.025	1.470	Y190	-	0.634	2.731	1.052
Y22	11, 21, 25	0.775	4.438	1.553	Y198	-	0.759	4.156	1.459
Y273	9, 23	0.667	3.007	1.206	Y201	-	0.682	3.149	1.351
Y23	9, 23	0.548	2.210	0.862	Y202	23	0.561	2.277	0.922
Y24	9, 23	0.591	2.443	0.964	Y203	11, 21	0.538	2.164	1.206
Y274	7, 16	0.593	2.458	1.025	Y207	-	0.597	2.479	0.976
Y03	17	0.676	3.084	1.252	Y208	-	0.229	1.297	0.390
Y275	6, 25	0.550	2.224	0.869	Y212	-	0.655	2.897	1.173
Y32	16	0.573	2.345	0.926	Y216	6, 25	0.753	4.042	1.412
Y40	4	0.254	1.340	0.421	Y277	-	0.700	3.335	1.283
Y48	9, 23	0.837	6.127	1.878	Y278	7	0.613	2.587	1.016
Y50	11, 18, 21	0.863	7.275	2.083	Y219	7	0.604	2.524	1.058
Y276	5, 10, 19	0.579	2.377	0.939	Y220	-	0.630	2.706	1.046
Y267	20	0.494	1.978	0.688	Y271	24	0.510	2.043	0.745
Y04	21	0.665	2.983	1.205	Y227	12, 26	0.577	2.366	0.933
Y268	6	0.557	2.257	0.912	Y10	21	0.563	2.290	0.904
Y66	20	0.489	1.958	0.682	Y231	3, 17	0.750	4.006	1.475
Y72	15	0.650	2.853	1.192	Y08	10, 11, 21	0.530	2.126	0.810
Y75	-	0.623	2.652	1.035	Y09	23	0.623	2.656	1.034
Y93	14	0.582	2.394	0.969	Y234	11	0.748	3.974	1.466
Y105	-	0.821	5.599	1.838	Y235	10, 20	0.598	2.489	0.980
Y269	-	0.781	4.573	1.568	Y236	11, 22	0.771	4.364	1.528
Y270	-	0.627	2.684	1.043	Y237	21	0.695	3.280	1.239
Y107	11	0.808	5.200	1.677	Y01	3	0.697	3.300	1.340
Y109	9	0.615	2.600	1.020	Y239	18	0.768	4.305	1.571
Y115	-	0.810	5.275	1.719	Y240	1	0.293	1.415	0.469
Y116	-	0.462	1.858	0.654	Y241	5	0.706	3.407	1.295
Y07	-	0.187	1.229	0.334	Y242	4, 22	0.441	1.790	0.633
Y119	-	0.681	3.135	1.289	Y245	22	0.663	2.967	1.202
Y120	-	0.633	2.723	1.050	Y246	23	0.620	2.629	1.027
Y128	-	0.620	2.629	1.027	Y247	23	0.622	2.645	1.029
Y137	-	0.591	2.443	0.964	Y248	-	0.249	1.331	0.415
Y05	-	0.507	2.029	0.744	Y250	-	0.604	2.525	1.063
Y142	-	0.813	5.350	1.774	Y251	25	0.557	2.257	0.911
Y152	-	0.779	4.515	1.562	Y255	-	0.307	1.444	0.486
Y159	-	0.823	5.653	1.786	Y258	10	0.665	2.984	1.206
Y160	-	0.573	2.345	0.966	Y260	-	0.666	2.993	1.097
Y163	-	0.663	2.971	1.191	Y261	-	0.612	2.579	1.014
Y167	-	0.621	2.638	0.715	Y265	-	0.564	2.291	0.901
Y168	-	0.500	2.000	0.693	Y266	-	0.565	2.297	0.904
Y170	-	0.419	1.721	0.610	Mean		0.611	2.919	1.083
Y171	-	0.485	1.941	0.678

引物 Primer	定位染色体 Chromosome	PIC	Ne	H'	引物 Primer	定位染色体 Chromosome	PIC	Ne	H'
Y12	24	0.626	2.672	1.030	Y172	-	0.653	2.885	1.107
Y272	13, 18	0.602	2.510	1.021	Y175	-	0.639	2.768	1.059
Y16	15	0.610	2.561	1.018	Y177	-	0.607	2.548	1.014
Y02	7, 13, 16	0.752	4.025	1.470	Y190	-	0.634	2.731	1.052
Y22	11, 21, 25	0.775	4.438	1.553	Y198	-	0.759	4.156	1.459
Y273	9, 23	0.667	3.007	1.206	Y201	-	0.682	3.149	1.351
Y23	9, 23	0.548	2.210	0.862	Y202	23	0.561	2.277	0.922
Y24	9, 23	0.591	2.443	0.964	Y203	11, 21	0.538	2.164	1.206
Y274	7, 16	0.593	2.458	1.025	Y207	-	0.597	2.479	0.976
Y03	17	0.676	3.084	1.252	Y208	-	0.229	1.297	0.390
Y275	6, 25	0.550	2.224	0.869	Y212	-	0.655	2.897	1.173
Y32	16	0.573	2.345	0.926	Y216	6, 25	0.753	4.042	1.412
Y40	4	0.254	1.340	0.421	Y277	-	0.700	3.335	1.283
Y48	9, 23	0.837	6.127	1.878	Y278	7	0.613	2.587	1.016
Y50	11, 18, 21	0.863	7.275	2.083	Y219	7	0.604	2.524	1.058
Y276	5, 10, 19	0.579	2.377	0.939	Y220	-	0.630	2.706	1.046
Y267	20	0.494	1.978	0.688	Y271	24	0.510	2.043	0.745
Y04	21	0.665	2.983	1.205	Y227	12, 26	0.577	2.366	0.933
Y268	6	0.557	2.257	0.912	Y10	21	0.563	2.290	0.904
Y66	20	0.489	1.958	0.682	Y231	3, 17	0.750	4.006	1.475
Y72	15	0.650	2.853	1.192	Y08	10, 11, 21	0.530	2.126	0.810
Y75	-	0.623	2.652	1.035	Y09	23	0.623	2.656	1.034
Y93	14	0.582	2.394	0.969	Y234	11	0.748	3.974	1.466
Y105	-	0.821	5.599	1.838	Y235	10, 20	0.598	2.489	0.980
Y269	-	0.781	4.573	1.568	Y236	11, 22	0.771	4.364	1.528
Y270	-	0.627	2.684	1.043	Y237	21	0.695	3.280	1.239
Y107	11	0.808	5.200	1.677	Y01	3	0.697	3.300	1.340
Y109	9	0.615	2.600	1.020	Y239	18	0.768	4.305	1.571
Y115	-	0.810	5.275	1.719	Y240	1	0.293	1.415	0.469
Y116	-	0.462	1.858	0.654	Y241	5	0.706	3.407	1.295
Y07	-	0.187	1.229	0.334	Y242	4, 22	0.441	1.790	0.633
Y119	-	0.681	3.135	1.289	Y245	22	0.663	2.967	1.202
Y120	-	0.633	2.723	1.050	Y246	23	0.620	2.629	1.027
Y128	-	0.620	2.629	1.027	Y247	23	0.622	2.645	1.029
Y137	-	0.591	2.443	0.964	Y248	-	0.249	1.331	0.415
Y05	-	0.507	2.029	0.744	Y250	-	0.604	2.525	1.063
Y142	-	0.813	5.350	1.774	Y251	25	0.557	2.257	0.911
Y152	-	0.779	4.515	1.562	Y255	-	0.307	1.444	0.486
Y159	-	0.823	5.653	1.786	Y258	10	0.665	2.984	1.206
Y160	-	0.573	2.345	0.966	Y260	-	0.666	2.993	1.097
Y163	-	0.663	2.971	1.191	Y261	-	0.612	2.579	1.014
Y167	-	0.621	2.638	0.715	Y265	-	0.564	2.291	0.901
Y168	-	0.500	2.000	0.693	Y266	-	0.565	2.297	0.904
Y170	-	0.419	1.721	0.610	Mean		0.611	2.919	1.083
Y171	-	0.485	1.941	0.678

	份数 No. of materials	等位变异数 Allele number	位点多态信息含量 PIC	有效等位基因数 Ne	基因型多样性 H′
耐盐材料 Salinity tolerant materials	23	3.784 (2-10)	0.613 (0.159-0.862)	2.929 (1.189-7.267)	1.083 (0.295-2.103)
盐敏感材料 Salinity sensitive materials	24	3.759 (2-8)	0.605 (0.198-0.860)	2.883 (1.246-7.143)	1.071 (0.349-2.025)