生物多样性 ›› 2019, Vol. 27 ›› Issue (10): 1122-1131.DOI: 10.17520/biods.2019180

• 研究报告:微生物多样性 • 上一篇    下一篇

新疆不同来源金黄壳囊孢的多样性

赵颖1,马荣1,尹永香2,张志东3,田呈明4,*()   

  1. 1 新疆农业大学林学与园艺学院, 乌鲁木齐 830052
    2 新疆农业大学林业研究所, 乌鲁木齐 830052
    3 新疆农业科学院微生物应用研究所, 乌鲁木齐 830091
    4 北京林业大学省部共建森林培育与保护教育部重点实验室, 北京 100083
  • 收稿日期:2019-05-29 接受日期:2019-08-09 出版日期:2019-10-20 发布日期:2019-10-20
  • 通讯作者: 田呈明
  • 基金资助:
    国家自然科学基金(31460198);中央高校基本科研业务费专项资金(2019GJZL01)

Diversity of Cytospora chrysosperma from different hosts in Xinjiang

Zhao Ying1,Ma Rong1,Yin Yongxiang2,Zhang Zhidong3,Tian Chengming4,*()   

  1. 1 College of Forestry and Horticulture, Xinjiang Agricultural University, Urumqi 830052
    2 Forestry Research Institute, Xinjiang Agricultural University, Urumqi 830052
    3 Institute of Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091
    4 Key Laboratory for Silviculture and Conservation of Ministry of Education College of Forestry, Beijing 100083
  • Received:2019-05-29 Accepted:2019-08-09 Online:2019-10-20 Published:2019-10-20
  • Contact: Tian Chengming

摘要:

为明确新疆不同寄主及地理来源的金黄壳囊孢(Cytospora chrysosperma)的多样性, 探讨种内亲缘关系和多样性差异。作者通过记录菌株在PDA培养基上的菌落颜色、形状、子实体形态等特征, 并应用Biolog-FF技术及ISSR分子标记技术, 比较了来自新疆5个地区5种寄主上的47株金黄壳囊孢的培养特征、生理生化特征及遗传多样性。结果表明47株金黄壳囊孢依据培养特征可划分为15种类型。不同类型菌株在碳源利用及代谢能力上存在差异, 各菌株对碳源的利用数量随着培养时间的增长逐渐增多。菌株882利用的碳源数量最多, 培养120 h可利用28种不同碳源, 碳源代谢能力中等; 菌株812-1利用的碳源数量最少, 培养120 h仅利用7种碳源, 代谢能力较低; 菌株1074-2、847、934、891-1、896、740具有单独利用碳源的能力。基于遗传相似性系数进行聚类分析, 结果显示遗传相似性系数为0.58时, 47个菌株被划分为两大类群, 其中第二类群菌株的培养特征为: 菌落白色、子实体较小且分布密集。供试金黄壳囊孢的多样性主要受自身遗传结构的影响, 不同寄主种类和地理来源对多样性的影响不显著。

关键词: 金黄壳囊孢, 培养性状, Biolog-FF技术, ISSR分子标记

Abstract

Cytospora chrysosperma is a very important pathogenic fungus that can be infects many different trees. Currently, the diversity, phylogenetic and genetic relationships of C. chrysosperma remain unclear as host differences could cause substantial genetic and diverse differences among C. chrysosperma strains. To analyze the genetic, phylogenetic, and current diversity of C. chrysosperma, physiological and biochemical characteristics and genetic tests were performed from different hosts in Xinjiang. Using molecular markings Biolog-FF and ISSR, 47 strains of C. chrysosperma have been divided into 15 types. Strains were cultured and displayed varying culture characteristics and carbon source utilization and metabolic capacity traits. Each strain tended to utilize more carbon over time, however, strains varied in their carbon source preferences and metabolic capacities. Using cluster analysis, 47 strains of C. chrysosperma divided into two clusters based on genetic similarity (0.58), suggesting genetic structure is essential to understanding C. chrysosperma diversity, as host species and geographical origins were not significant.

Key words: Cytospora chrysosperma, cultural characteristics, Biolog-FF, ISSR