生物多样性 ›› 2011, Vol. 19 ›› Issue (5): 535-542.DOI: 10.3724/SP.J.1003.2011.08109

• 研究报告 • 上一篇    下一篇

能源作物芒属双药芒组SSR引物的筛选及其评价

马洪峥1,2, 李珊珊2, 葛颂2, 戴思兰1,*(), 陈文俐2,*()   

  1. 1北京林业大学园林学院, 北京 100083
    2中国科学院植物研究所系统与进化植物学国家重点实验室, 北京 100093
  • 收稿日期:2011-07-11 接受日期:2011-08-24 出版日期:2011-09-20 发布日期:2011-10-08
  • 通讯作者: 戴思兰,陈文俐
  • 作者简介:*E-mail: silandai@gmail.com, chenwl@ibcas.ac.cn
  • 基金资助:
    中国科学院知识创新工程重要方向项目(KZCX2-YW-034);中国科学院知识创新工程重要方向项目(KSCX2-YW-Z-0943)

Isolation of SSR markers for two related second-generation energy crop species, Miscanthus nepalensis and M. nudipes (Poaceae)

Ma Hongzheng1,2, Li Shanshan2, Ge Song2, Dai Silan1,*(), Chen Wenli2,*()   

  1. 1College of Landscape Architecture, Beijing Forestry University, Beijing 100083
    2State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing 100093
  • Received:2011-07-11 Accepted:2011-08-24 Online:2011-09-20 Published:2011-10-08
  • Contact: Dai Silan,Chen Wenli

摘要:

禾本科芒属植物具有在边际性土地(marginal land)上驯化成为高产能源作物的巨大潜力, 其中尼泊尔芒(Miscanthus nepalensis)和双药芒(M. nudipes)两个双药芒组物种分布于喜马拉雅-横断山地区, 是具有耐寒及抗旱特性的优良种质资源。为了了解其遗传多样性和群体遗传结构,需要筛选出有效的分子标记。目前在适用于核心芒属植物群体遗传学分析的42对SSR引物中, 有23对来自于核心芒属物种,19对来自玉米。我们利用来自双药芒组2个种的6个代表性个体, 筛选得到14对可在双药芒组中稳定扩增的引物, 其中有12对来自核心芒属,显示同属引物的扩增效率为52.2%(12/23)。成功扩增的14个位点中11个为多态。利用所得引物对双药芒组4个自然居群进行群体遗传学初步分析发现, 所获得的SSR引物多态性高、表现稳定, 适于双药芒组物种的群体遗传多样性分析, 可为芒属植物资源的研究和利用提供重要的分子标记。

关键词: Miscanthus nepalensis, M. nudipes, SSR引物, 能源作物, 多态性

Abstract

Miscanthusspecies have shown great potential as second-generation energy crops, especially when planted on marginal land. Within the genus, M. nepalensisand M. nudipes are much different from other species owing to the presence of only two anthers, and are thus treated as sect. Diandra Keng taxonomically. Distributed in the Himalaya and Hengduan Mountains, the two species exhibit a very high level of tolerance to cold and drought stresses. In order to scientifically develop these crop species, the first step would be to carry out a population-genetic investigation to elucidate the genetic basis of phenotypes such as tolerance-related and morphological characteristics. For this purpose, we need to select practical and high-resolution molecular markers. We sequenced six individuals of M. nepalensisandM. nudipes to validate the efficacious use of 42 previously-used SSR primer pairs. A total of 14 primer pairs were identified as applicable, of which 12 were from Miscanthusspecies, indicating that the amplification ratio within genus Miscanthus is 52.2% (12/23). Of the 14 primer pairs, 11 were polymorphic. Screening four natural populations of M. nepalensisand M. nudipesusing the 14 SSR markers, we found that these primers had high power in detecting genetic diversity and population genetic structure in M. nepalensisand M. nudipes, and thus provide feasible genetic markers for population genetics studies and molecular breeding.

Key words: Miscanthus nepalensis, Miscanthus nudipes, SSR primers, energy crop, polymorphism