The application of UAV technology brings new opportunities and revolutions to the monitoring and research on biodiversity in protected area. However, we find that no monitoring technology solutions combining UAV and ground approaches have been formed yet, due to the lack of concerns on protected objects. Considering to better perform various monitoring technology strengths and to effectively support management and monitoring in protected area, we review study cases on UAV and ground investigation domestic and overseas firstly, and then compare the demands from conservation, management and monitoring of all kinds protected area in China. In general, the ecosystems, species, site relics and landscape are regarded as protected objects in all kinds of protected area. Meanwhile, conservation, recovering, study, education, recreation, and sustainable development become the management goals. Based on the demands mentioned above, we present an integrated technology solution which composed of four categories and 14 subjects for UAV and ground to monitor biodiversity coherently. This solution includes image recognition and classification, data inversion and pattern analysis, digital modeling and surface measuring, patrolling and inspection. In addition, monitoring time and frequency, index, integration approach, data postprocessing can be acquired in the solution. Furthermore, monitoring subjects were chosen to apply and test in the Three Parallel Rivers World Heritage, such as plant identification, vegetation growth, landscape pattern, surface measuring, and law enforcement. While achieving good results on the solution verification, we hope that this monitoring solution will do significant help to improve the protected area biodiversity conservation and management level, also be part of technological storage in assessment and supervision.