生物多样性 ›› 2016, Vol. 24 ›› Issue (10): 1164-1176.doi: 10.17520/biods.2016085

• • 上一篇    下一篇

武夷山自然保护区土壤可培养芽胞杆菌 的物种多样性及分布

葛慈斌, 郑榕, 刘波*(), 刘国红, 车建美, 唐建阳   

  1. 福建省农业科学院农业生物资源研究所, 福州 350003
  • 收稿日期:2016-03-27 接受日期:2016-09-02 出版日期:2016-10-20
  • 通讯作者: 刘波 E-mail:fzliubo@163.com
  • 基金项目:
    国家自然科学基金(31370059)、福建省省属公益类科研院所专项(2015R1018-12)、公益性行业(农业)科研专项(201303094)和科技部国际合作项目(2012DFA31120)

Diversity and distribution of cultivable Bacillus-like species in soils collected from Wuyishan Nature Reserve

Cibin Ge, Rong Zheng, Bo Liu*(), Guohong Liu, Jianmei Che, Jianyang Tang   

  1. Institute of Agrobiological Resources, Fujian Academy of Agricultural Sciences, Fuzhou 350003
  • Received:2016-03-27 Accepted:2016-09-02 Online:2016-10-20
  • Contact: Liu Bo E-mail:fzliubo@163.com

为了解武夷山自然保护区土壤中可培养芽胞杆菌的分布状况, 2012年6月从该保护区的黄岗山顶部、中部、底部和桐木关、挂墩、大竹岚等6个地点采集土样75份。用80℃水浴加热、稀释平板法进行芽胞杆菌的分离, 并根据16S rRNA基因序列分析对菌株进行初步鉴定。从土样中分离出芽胞杆菌418株, 鉴定为8个属42个种, 其中Bacillus属的种数最多, 有20种, Paenibacillus属和Lysinibacillus属分别有8种和7种。不同地点分离到的芽胞杆菌在种类、数量上存在差异: 从大竹岚土壤中分离到的芽胞杆菌种类最多, 从黄岗山中部和底部分离到的种类数则较少; 挂墩、大竹岚土壤中芽胞杆菌的数量较大, 达3.6×106 cfu/g以上, 而黄岗山顶部和中部土壤中的数量则少于4.9×105 cfu/g。Bacillus cereusB. mycoidesB. thuringiensisLysinibacillus xylanilyticus等4个种在6个地点的土样中均有分离到, 其中B. thuringiensisL. xylanilyticus是该保护区土壤中的优势种。桐木关土壤中芽胞杆菌的种类多样性和均匀度指数都比其他5个地点的高, 而挂墩土壤中芽胞杆菌的Shannon-Wiener多样性、均匀度和优势度指数都最低。B. mycoidesB. thuringiensis的数量与海拔显著相关, 相关系数分别为0.852和-0.834, B. cereusB. mycoidesB. thuringiensis的分离频度与海拔的相关性极显著, 相关系数分别为0.960、0.952和-0.931。研究结果表明, 武夷山自然保护区土壤中可培养芽胞杆菌的种类丰富、数量较大, 具有较高的多样性。

关键词: 武夷山自然保护区, 芽胞杆菌, 16S rRNA, 多样性, 分离频度

The present paper reported the distribution and diversity of cultivable Bacillus-like species in soils from Wuyishan National Nature Reserve. In June 2012, 75 soil samples were collected from the peak, middle and base of the Huanggang Mountain, Tongmuguan, Guadun, and Dazhulan in the reserve. The Bacillus-like species were isolated by plating the series dilution after 80℃water bath heating the soil samples and then identified using 16S rRNA gene sequence analysis. Results showed that 418 strains isolated from the soil samples belonged to 42 Bacillus-like species, which were grouped into eight genera containing Bacillus, Brevibacillus, Fictibacillus, Lysinibacillus, Paenibacillus, Psychrobacillu, Rummeliibacillus and Viridibacillus. Furthermore, Bacillus was the dominant genus, followed by the genera Paenibacillus and Lysinibacillus. The greatest number of Bacillus-like species was isolated from the soils in Dazhulan, while the fewest were found at the middle and base of Huanggang Mountain. The quantities of Bacillus-like species in soils collected from Guadun and Dazhulan were more than 3.6×106 cfu/g soil, but less than 4.9×105 cfu/g soil at the top and middle of Huanggang Mountain. Bacillus cereus, B. mycoides, B. thuringiensis, and Lysinibacillus xylanilyticus were isolated from all six sites, which were dominated by B. thuringiensis and L. xylanilyticus. The Shannon-Wiener diversity index and Pielou’s evenness index for Bacillus-like species isolated from Tongmuguan were higher than those at the other five sites, while the lowest Shannon-Wiener diversity index, Pielou’s evenness index and Simpson index values were found in the soils from Guadun. There was a significant correlation between the altitude and the quantity of B. mycoides and B. thuringiensis, with correlations of 0.852 and -0.834, respectively. Additionally, highly significant correlations were found between the altitude and the isolating frequency of B. cereus, B. mycoides and B. thuringiensis, with correlations of 0.960, 0.952 and -0.931, respectively. These results indicated the abundance and diversity of the Bacillus-like species community and bacteria species were extremely rich in Wuyishan Nature Reserve.

Key words: Wuyishan Nature Reserve, Bacillus-like species, 16S rRNA, diversity, isolating frequency

表1

采自武夷山自然保护区的土壤样品信息"

采集地点
Location
海拔
Altitude (m)
土壤样品数
No. of samples
生境类型
Habitat
土壤类型
Agrotype
黄岗山顶部
Peak of the Huanggang Mountain (PHM)
2,158 15 草甸、岩石
Meadow, rock
山地草甸土
Mountain meadow soil
黄岗山中部
Middle of the Huanggang Mountain (MHM)
1,700 13 树林、草丛、路边等
Forest, grass, roadside
黄壤
Yellow soil
黄岗山底部
Base of the Huanggang Mountain (BHM)
1,100 7 树林、草丛、路边等
Forest, grass, roadside
红黄壤、黄壤
Red and yellow soil, yellow soil
桐木关
Tongmuguan (TMG)
890 12 树林、草丛、路边等
Forest, grass, roadside
红黄壤
Red and yellow soil
挂墩
Guadun (GD)
1,200 14 树林、草丛、路边等
Forest, grass, roadside
红黄壤、黄壤
Red and yellow soil, yellow soil
大竹岚
Dazhulan (DZL)
1,000 14 树林、草丛
Forest, grass
红黄壤、黄壤
Red and yellow soil, yellow soil

表2

从武夷山自然保护区分离得到的芽胞杆菌的种类概况"

类群
Group
代表菌株
Strains
登录号
GenBank
accession no.
最相近菌种
Closest match
相似性
Sequence
identity (%)
芽胞杆菌属 Bacillus FJAT-16209 KF278157 阿氏芽胞杆菌 Bacillus aryabhattai 100.0
FJAT-16883 KF278197 巴达维亚芽胞杆菌 B. bataviensis 100.0
FJAT-16354 KF278179 科研中心芽胞杆菌 B. cecembensis 95.95
FJAT-16889 KF278199 蜡样芽胞杆菌 B. cereus 100.0
FJAT-16469 KF278181 根内芽胞杆菌 B. endoradicis 99.12
FJAT-16705 KF278193 盐敏芽胞杆菌 B. halmapalus 98.52
FJAT-16532 KF278187 印空研芽胞杆菌 B. isronensis 100.0
FJAT-16048 KF278123 万里浦芽胞杆菌 B. manliponensis 98.46
FJAT-16291 KF278168 地衣芽胞杆菌 B. licheniformis 99.85
FJAT-16872 KF278195 黄海芽胞杆菌 B. marisflavi 100.0
FJAT-16134 KF278130 甲基营养型芽胞杆菌 B. methylotrophicus 99.86
FJAT-16162 KF278147 壁芽胞杆菌 B. muralis 99.85
FJAT-16884 KF278198 蕈状芽胞杆菌 B. mycoides 100.0
FJAT-16339 KF278175 休闲地芽胞杆菌 B. novalis 99.83
FJAT-16201 KF278155 假蕈状芽胞杆菌 B. pseudomycoides 99.85
FJAT-16578 KF278189 沙福芽胞杆菌 B. safensis 100.0
FJAT-17017 KF278204 简单芽胞杆菌 B. simplex 100.0
FJAT-17042 KF278206 特基拉芽胞杆菌 B. tequilensis 99.89
FJAT-16178 KF278153 苏云金芽胞杆菌 B. thuringiensis 100.0
FJAT-16301 KF278171 韦氏芽胞杆菌 B. weihenstephanensis 100.0
短芽胞杆菌属 Brevibacillus FJAT-16350 KF278177 土壤短芽胞杆菌 Brevibacillus agri 99.38
虚构芽胞杆菌属 Fictibacillus FJAT-17014 KF278203 南海虚构芽胞杆菌 Fictibacillus nanhaiensis 100.0
赖氨酸芽胞杆菌属 Lysinibacillus FJAT-16156 KF278145 纺锤形赖氨酸芽胞杆菌 Lysinibacillus fusiformis 100.0
FJAT-16167 KF278149 延长赖氨酸芽胞杆菌 L. macroides 99.57
FJAT-16506 KF278185 芒果土赖氨酸芽胞杆菌 L. mangiferahumi 99.98
FJAT-16266 KF278163 马赛赖氨酸芽胞杆菌 L. massiliensis 97.51
FJAT-16248 KF278158 低硼赖氨酸芽胞杆菌 L. parviboronicapiens 99.22
FJAT-16141 KF278135 球形赖氨酸芽胞杆菌 L. sphaericus 99.14
FJAT-16140 KF278134 解木糖赖氨酸芽胞杆菌 L. xylanilyticus 100.0
类芽胞杆菌属 Paenibacillus FJAT-16151 KF278140 蜂房类芽胞杆菌 Paenibacillus alvei 98.82
FJAT-16703 KF278192 蜜蜂类芽胞杆菌 P. apiarius 98.88
FJAT-16893 KF278200 栗树类芽胞杆菌 P. castaneae 98.05
FJAT-16129 KF278128 埃及类芽胞杆菌 P. elgii 99.40
FJAT-16135 KF278131 灿烂类芽胞杆菌 P. lautus 99.13
FJAT-16879 KF278196 松树类芽胞杆菌 P. pini 99.98
FJAT-16903 KF278201 台中类芽胞杆菌 P. taichungensis 100.0
FJAT-16927 KF278202 土地类芽胞杆菌 P. terrigena 97.61
嗜冷芽胞杆菌属 Psychrobacillus FJAT-16132 KF278129 奇特嗜冷芽胞杆菌 Psychrobacillus insolitus 98.70
FJAT-16497 KF278183 忍冷嗜冷芽胞杆菌 P. psychrodurans 99.82
鲁氏芽胞杆菌属 Rummeliibacillus FJAT-16098 KF278125 厚细胞鲁氏芽胞杆菌 Rummeliibacillus pycnus 98.39
绿芽胞杆菌属 Viridibacillus FJAT-17036 KF278205 沙地绿芽胞杆菌 Viridibacillus arenosi 100.0
FJAT-16340 KF278176 田地绿芽胞杆菌 V. arvi 100.0

图1

基于16S rRNA基因序列的武夷山自然保护区芽胞杆菌系统发育树。数字表示各节点的自展支持率数值(>50%)。"

表3

武夷山自然保护区各地区芽胞杆菌的分离频度(%)和平均数量(×105 cfu/g)"

芽胞杆菌种类
Bacillus-like species
黄岗山顶部
Peak of the Huanggang Mountain
黄岗山中部
Middle of the Huanggang Mountain
黄岗山底部
Base of the Huanggang Mountain
桐木关
Tongmuguan
挂墩
Guadun
大竹岚
Dazhulan
总体
Total
频度
Frequency
数量
Quantification
频度
Frequency
数量
Quantification
频度
Frequency
数量
Quantification
频度
Frequ-ency
数量
Quantification
频度
Frequency
数量
Quantification
频度
Frequency
数量
Quantification
频度
Frequency
数量
Quantification
Bacillus aryabhattai 0 0 7.69 0.02 14.29 0.10 8.33 2.42 14.29 0.05 14.29 0.62 9.33 3.20
B. bataviensis 0 0 0 0 0 0 0 0 0 0 7.14 0.43 1.33 0.43
B. cecembensis 6.67 0.01 0 0 0 0 0 0 0 0 0 0 1.33 0.01
B. cereus 66.67 0.26 53.85 1.27 28.57 0.72 16.67 0.29 42.86 0.64 21.43 0.40 40 3.58
B. endoradicis 13.33 0.02 7.69 0.02 0 0 0 0 0 0 0 0 4.00 0.03
B. halmapalus 0 0 0 0 0 0 0 0 7.14 0.01 0 0 1.33 0.01
B. isronensis 6.67 0.01 0 0 0 0 0 0 0 0 0 0 1.33 0.01
B. manliponensis 0 0 0 0 0 0 0 0 14.29 0.07 0 0 2.67 0.07
B. licheniformis 6.67 0.03 0 0 0 0 0 0 0 0 0 0 1.33 0.03
B. marisflavi 0 0 0 0 0 0 0 0 0 0 7.14 0.11 1.33 0.11
B. methylotrophicus 6.67 0.01 0 0 0 0 8.33 0.01 0 0 0 0 2.67 0.02
B. muralis 0 0 0 0 0 0 25.00 0.67 0 0 14.29 0.77 6.67 1.43
B. mycoides 100.0 3.26 69.20 1.42 28.57 0.95 16.67 0.47 7.14 0.04 14.29 1.24 41.33 7.37
B. novalis 6.67 0.07 0 0 0 0 0 0 0 0 0 0 1.33 0.07
B. pseudomycoides 0 0 0 0 28.57 0.21 8.33 0.42 42.86 2.44 14.29 0.09 14.67 3.16
B. safensis 0 0 7.69 0.01 0 0 0 0 14.29 0.36 0 0 4.00 0.36
B. simplex 26.67 0.18 0 0 0 0 0 0 0 0 7.14 0.05 6.67 0.23
B. tequilensis 0 0 0 0 0 0 0 0 0 0 7.14 16.19 1.33 16.19
B. thuringiensis 20 0.18 30.77 0.63 100.0 6.73 83.33 11.00 71.43 3.24 85.71 13.47 61.33 35.25
B. weihenstephanensis 0 0 7.69 0.02 0 0 0 0 0 0 0 0 1.33 0.02
Brevibacillus agri 6.67 0.19 0 0 0 0 0 0 0 0 0 0 1.33 0.19
Fictibacillus nanhaiensis 0 0 0 0 0 0 0 0 0 0 7.14 0.10 1.33 0.10
Lysinibacillus fusiformis 0 0 0 0 28.57 0.31 8.33 0.58 0 0 0 0 4.00 0.89
L. macroides 6.67 0.02 0 0 0 0 8.33 0.08 0 0 0 0 2.67 0.10
L. mangiferahumi 13.33 0.15 0 0 0 0 0 0 7.14 0.04 0 0 4.00 0.19
L. massiliensis 0 0 0 0 14.29 0.14 0 0 0 0 0 0 1.33 0.14
L. parviboronicapiens 0 0 0 0 28.57 1.29 16.67 0.65 0 0 0 0 5.33 1.93
L. sphaericus 0 0 0 0 0 0 8.33 0.13 7.14 0.04 21.43 0.87 6.67 1.03
L. xylanilyticus 60 0.27 30.77 0.11 57.14 0.74 58.33 3.68 78.57 3.22 21.43 1.08 50.67 9.10
Paenibacillus alvei 0 0 0 0 14.29 0.03 0 0 7.14 28.57 0 0 2.67 28.60
P. apiarius 0 0 0 0 0 0 0 0 7.14 0.31 0 0 1.33 0.31
P. castaneae 0 0 0 0 0 0 0 0 0 0 7.14 0.04 1.33 0.04
P. elgii 0 0 0 0 0 0 0 0 7.14 0.01 0 0 1.33 0.01
P. lautus 0 0 0 0 0 0 8.33 0.05 0 0 0 0 1.33 0.05
P. pini 0 0 0 0 0 0 0 0 0 0 7.14 0.57 1.33 0.57
P. taichungensis 0 0 0 0 0 0 8.33 0.07 0 0 7.14 0.48 2.67 0.54
P. terrigena 0 0 0 0 0 0 0 0 0 0 7.14 0.01 1.33 0.01
Psychrobacillus insolitus 0 0 0 0 0 0 8.33 0.31 0 0 0 0 1.33 0.31
Psychrobacillus psychrodurans 13.33 0.11 7.69 0.01 0 0 0 0 0 0 0 0 4.00 0.12
Rummeliibacillus pycnus 0 0 0 0 0 0 0 0 64.29 0.64 0 0 12.00 0.64
Viridibacillus arenosi 26.67 0.11 0 0 0 0 8.33 1.33 14.29 0.11 14.29 0.31 12.00 1.86
V. arvi 20 0.01 7.69 0.01 0 0 0 0 0 0 0 0 5.33 0.02
总计 Total - 4.866 - 3.485 - 11.222 - 22.137 - 39.761 - 36.795 - -

图2

武夷山保护区不同采样地点土壤芽胞杆菌的多样性指数。PHM、MHM、BHM、TMG、GD、DZL的含义见表1。"

表4

武夷山自然保护区芽胞杆菌数量、分离频度、海拔间的相关性"

芽胞杆菌种类
Bacillus-like species
数量与海拔的相关性
Correlation between quantification
and altitude
分离频度与海拔的相关性
Correlation between frequency
and altitude
数量与分离频度的相关性
Correlation between quantification
and frequency
Bacillus cereus 0.136 0.960** 0.220
Bacillus mycoides 0.852* 0.952** 0.906*
Bacillus thuringiensis -0.834* -0.931** 0.792
Lysinibacillus xylanilyticus -0.635 0.034 -0.273

图3

芽胞杆菌种类分布的聚类分析"

1 Chen S, Hu W, Xiao Y, Deng Y, Jia J, Hu M (2012) Degradation of 3-phenoxybenzoic acid by aBacillus sp. PLoS ONE, 7, e50456.
2 Eduardo EC, José RA, José ÁVQ, María MSR, Juan AED, Humberto GR, César MCA (2015) Classification and ordination of main plant communities along an altitudinal gradient in the arid and temperate climates of northeastern Mexico. The Science of Nature, 102, 58-68.
3 Fang YH (2005) Species composition and diversity of evergreen broad-leaved forest of Castanopsis carlesii and C. eyrei in Wuyishan National Nature Reserve, Fujian, China. Biodiversity Science, 13, 148-155. (in Chinese with English abstract)
[方燕鸿 (2005) 武夷山米槠、甜槠常绿阔叶林的物种组成及多样性分析. 生物多样性, 13, 148-155.]
4 Fierer N, Mccain CM, Meir P, Zimmermann M, Rapp JM, Silman MR, Knight R (2011) Microbes do not follow the elevational diversity patterns of plants and animals. Ecology, 92, 797-804.
5 Gartner A, Blumel M, Wiese J, Imhoff JF (2011) Isolation and characterisation of bacteria from the Eastern Mediterranean deep sea. Antonie Van Leeuwenhoek, 100, 421-435.
6 Ge CB, Liu B, Che JM, Chen MC, Liu GH, Wei JC (2015) Diversity of Bacillus species inhabiting on the surface and endophyte of lichens collected from Wuyi Mountain. Acta Microbiologica Sinica, 55, 551-563. (in Chinese with English abstract)
[葛慈斌, 刘波, 车建美, 陈梅春, 刘国红, 魏江春 (2015) 武夷山地衣表生和内生芽胞杆菌种群的多样性. 微生物学报, 55, 551-563.]
7 Guo XH, Zhao ZD, Xiong HR (2010) Review on the intestinal microecology of Bacilli-derived probiotics. Chinese Journal of Microecology, 22, 1136-1139. (in Chinese with English abstract)
[郭小华, 赵志丹, 熊海容 (2010) 益生芽胞杆菌肠道微生态学研究进展. 中国微生态学杂志, 22, 1136-1139.]
8 Hayat R, Rizwan AS, Muhammad IH, Ahmed I (2013) Characterization and identification of compost bacteria based on 16S rRNA gene sequencing. Annals of Microbiology, 63, 905-912.
9 Hu LL, Yan BQ, Jiang MX, Zhu JJ (2007) The diversity of plant communities with endangered plant, Berchemiella wilsonii vat. pubipetiolata. Acta Botanica Boreali- Occidentalia Sinica, 27, 594-600. (in Chinese with English abstract)
[胡理乐, 闫伯前, 江明喜, 朱教君 (2007) 毛柄小勾儿茶伴生群落种类组成及多样性研究. 西北植物学报, 27, 594-600.]
10 José AS, Rosa M (2016) Abundance and diversity of bacterial, archaeal, and fungal communities along an altitudinal gradient in alpine forest soils: what are the driving factors? Microbial Ecology, 72, 207-220.
11 Kim M, Oh HS, Park SC, Chun J (2014) Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes. International Journal of Systematic and Evolutionary Microbiology, 64, 346-351.
12 Kim OS, Cho YJ, Lee K, Yoon SH, Kim M, Na H, Park SC, Lee JH, Yi H, Won S, Chun J (2012) Introducing Eztaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. International Journal of Systematic and Evolutionary Microbiology, 62, 716-721.
13 Kim YH, Kim IS, Moon EY, Park JS, Kim SJ, Lim JH, Park BT, Lee EJ (2011) High abundance and role of antifungal bacteria in compost-treated soils in a wildfire area. Microbial Ecology, 62, 725-737.
14 Köberl M, Müller H, Ramadan EM, Berg G (2011) Desert farming benefits from microbial potential in arid soils and promotes diversity and plant health. PLoS ONE, 6, 244-252.
15 Lee CS, Jung YT, Park S, Oh TK, Yoon JH (2010) Lysibibacillus xylanilyticus sp. nov., a xalan-degrading bacterium isolated from forest humus. International Journal of Systematic and Evolutionary Microbiology, 60, 281-286.
16 Li XM, Che KJ, Yang YH, Wang H, Ma WW, Wang H, Huang R (2014) Variation pattern of soil nutrients in forests at dif- ferent altitudes at upstream of Bailongjiang River. Journal of Gansu Agricultural University, 49(6), 131-137. (in Chinese with English abstract)
[李兴民, 车克钧, 杨永红, 王辉, 马维维, 王惠, 黄蓉 (2014) 白龙江上游不同海拔森林土壤养分变化规律研究. 甘肃农业大学学报, 49(6), 131-137.]
17 Li ZJ, Chen LZ, Lin QX, Lin JL, Liu DL, Liu CD, He JY, Chen BH, Huang ZH, Lin WQ, Shi DM (2002) Study on the species diversity of higher plants in Buxus sinica var. parvifolia dwarf community in Wuyishan Mountains. Journal of Xiamen University (Natural Science), 41, 574-578. (in Chinese with English abstract)
[李振基, 陈鹭真, 林清贤, 林建丽, 刘德龙, 刘初钿, 何健源, 陈炳华, 黄泽豪, 林文群, 石冬梅 (2002) 武夷山自然保护区生物多样性研究. I. 小叶黄杨矮曲林物种多样性. 厦门大学学报(自然科学版), 41, 574-578.]
18 Liu M, Cui Y, Huang HQ, Sun QG, Zhu J, Zou XX, Bao SX (2014) Isolation and diversity of Bacillus-like species from Dongzhai Harbor mangrove soil. Journal of Microbiology, 34(5), 21-26. (in Chinese with English abstract)
[刘敏, 崔莹, 黄惠琴, 孙前光, 朱军, 邹潇潇, 鲍时翔 (2014) 东寨港红树林土壤芽胞杆菌分离及其多样性分析. 微生物学杂志, 34(5), 21-26.]
19 Lu H, Cong J, Liu X, Wang XL, Tang J, Li DQ, Zhang YG (2015) Plant diversity patterns along altitudinal gradients in alpine meadows in the Three River Headwater region, China. Acta Prataculturae Sinica, 24(7), 197-204. (in Chinese with English abstract)
[卢慧, 丛静, 刘晓, 王秀磊, 唐军, 李迪强, 张于光 (2015) 三江源区高寒草甸植物多样性的海拔分布格局. 草业学报, 24(7), 197-204.]
20 Lugo MA, Ferrero M, Menoyo E, Estévez MC, Siñeriz F, Anton A (2008) Arbuscular mycorrhizal fungi and rhizospheric bacteria diversity along an altitudinal gradient in South American Puna grassland. Microbial Ecology, 55, 705-713.
21 Sanahuja G, Banakar R, Twyman RM, Capell T, Christou P (2011) Bacillus thuringiensis: a century of research, devel- opment and commercial applications. Plant Biotechnology Journal, 9, 283-300.
22 Schnepf E, Crickmore N, van Rie J, Lereclus D, Baum J, Feitelson J, Zeigler DR, Dean DH (1998) Bacillus thuringiensis and its pesticidal crystal protein. Microbiology and Molecular Biology Reviews, 62, 775-806.
23 Singh D, Lee CL, Kim WS, Kerfahi D, Chun JH, Adams JM (2014) Strong elevational trends in soil bacterial community composition on Mt. Halla, South Korea. Soil Biology and Biochemistry, 68, 140-149.
24 Song J, Du LX, Wang RY, Wei LM, Cao WP, Song J, Wang JY, Feng SL (2011) Research on the distribution and diversity of Bacillus thuringiensis from Damaoshan Mountains. Chinese Agricultural Science Bulletin, 27, 166-169. (in Chinese with English abstract)
[宋健, 杜立新, 王容燕, 魏利民, 曹伟平, 宋健, 王金耀, 冯书亮 (2011) 大茂山地区苏云金芽胞杆菌分布与多样性研究. 中国农学通报, 27(1), 166-169.]
25 Song ZQ, Wang L, Liu XH, Lv G, Yang Q, Liang F (2013) Genetic diversity of Bacillus in agricultural soils in Henan Province. Journal of Henan Agricultural Sciences, 42(9), 73-78. (in Chinese with English abstract)
[宋兆齐, 王莉, 刘秀花, 吕刚, 杨清, 梁峰 (2013) 河南省农田土壤芽胞杆菌的遗传多样性分析. 河南农业科学, 42(9), 73-78.]
26 Sophie T, Hu XM, Jacques M (2011) Characterization of Bacilli isolated from the confined environments of the Antarctic Concordia Station and the International Space Station. Astrobiology, 11, 323-334.
27 Sun HG, Huang HL, Zheng J, Jin X (2012) Optimization of fermentation conditions for high production of antimicrobial substance by Bacillus mycoides SH-1. Journal of Xuzhou Institute of Technology (Natural Sciences), 27(2), 45-49. (in Chinese with English abstract)
[孙会刚, 黄海亮, 郑进, 金鑫 (2012) 蕈状芽胞杆菌SH-1抗菌活性物质发酵条件优化. 徐州工程学院学报(自然科学版), 27(2), 45-49.]
28 Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution, 30, 2725-2729.
29 Tang QY (2010) Data Processing System. Beijing: Science Press.
[唐启义 (2010) DPS数据处理系统. 北京; 科学出版社.]
30 Tang ZY, Gong GS, Liu P, Shao BL, Zhang SR (2009) A preliminary study of soil Bacillus in the suburbs of Chengdu. Journal of Southwest Agricultural University (Natural Science), 27, 188-192. (in Chinese with English abstract)
[唐志燕, 龚国淑, 刘萍, 邵宝林, 张世熔 (2009)成都市郊区土壤芽胞杆菌的初步研究. 西南农业大学学报(自然科学版), 27, 188-192.]
31 Timmell TE (1967) Recent progress in the chemistry of wood hemicelluloses. Wood Sciences Technology, 1, 45-70.
32 Trick I, Salcher O, Lingens F (1984) Characterization of filament forming Bacillus strains isolated from bulking sludge. Applied Microbiology and Biotechnology, 19, 120-124.
33 Vasudevan G, Siddarthan V, Ramatchandirane PS (2015) Predominance of Bacillus sp. in soil samples of the southern regions of Western Ghats, India. Annals of Microbiology, 65, 431-441.
34 Wang GX, Fu WF, Cui J, Yuan M, Yao L (2006) Effects of active components from Bacillus mycoides metabolite on immune functions of mice. Chinese Veterinary Science, 36, 983-987. (in Chinese with English abstract)
[王高学, 付维法, 崔婧, 袁明, 姚璐 (2006) 蕈状芽胞杆菌代谢产物活性成分对小鼠免疫功能的影响. 中国兽医科学, 36, 983-987.]
35 Wang JS (2007) The species diversity of subfamily Nymphulinae (Lepidoptera: Nymphulinae) communities in the Wuyishan Nature Reserve in Fujian, China. Entomological Journal of East China, 16(1), 59-63. (in Chinese with English abstract)
[汪家社 (2007) 武夷山自然保护区水螟亚科昆虫物种多样性研究. 华东昆虫学报, 16(1), 59-63.]
36 Wang JS (2006) The species diversity of subfamily Pyralinae (Lepidoptera: Pyralinae) communities in Wuyishan Nature Reserve. Journal of Nanjing Forestry University (Natural Sciences), 30(3), 98-100. (in Chinese with English abstract)
[汪家社 (2006) 武夷山自然保护区螟蛾亚科昆虫的物种多样性. 南京林业大学学报(自然科学版), 30(3), 98-100.]
37 Wang JT, Cao P, Hu HW, Li J, Han LL, Zhang LM, Zheng YM, He JZ (2015) Altitudinal distribution patterns of soil bacterial and archaeal communities along Mt. Shegyla on the Tibetan Plateau. Microbial Ecology, 69, 135-145.
38 Wang K, Yan PS, Ding QL, Wu QX, Wang ZB, Peng J (2013) Diversity of culturable root-associated/endophytic bacteria and their chitinolytic and aflatoxin inhibition activity of peanut plant in China. World Journal of Microbiology and Biotechnology, 29, 1-10.
39 Wang LG, Xu C (2010) Preliminary discussion on the plant of Magnoliaceae in Wuyishan Nature Reserve. Journal of Fujian Forestry Science and Technology(福建林业科技), 37(2), 90-93. (in Chinese with English abstract)
[王良桂, 徐晨 (2010) 福建武夷山国家级自然保护区木兰科植物资源初探. 福建林业科技, 37(2), 90-93.]
40 Wang SJ, Ruan HH, Wang JS, Xu ZK, Wu YY (2010) Dynamic change of soil fauna community structure in the course of litter decomposition on the Wuyi Mountains. Journal of Southwest Forestry University, 30(6), 43-47. (in Chinese with English abstract)
[王邵军, 阮宏华, 汪家社, 徐自坤, 吴焰玉 (2010) 武夷山土壤动物群落结构在凋落物分解过程中的变化. 西南林学院学报, 30(6), 43-47.]
41 Wang ZX, Liu B, Lin YZ, Liu GH (2012) Collection, identification and phylogenetic diversity of Bacillus species in soil samples from Xinjiang. Fujian Journal of Agricultural Sciences, 27, 187-195. (in Chinese with English abstract)
[王子旋, 刘波, 林营志, 刘国红 (2012) 新疆土壤芽胞杆菌采集鉴定及其分布多样性. 福建农业学报, 27, 187-195.]
42 Wei X, Zheng XF, Zhang SX (2014) Forest soil physicochemical properties along different altitudinal gradients at Huoditang in the Qinling Mountains. Journal of Northwest Forestry University, 29(3), 9-14. (in Chinese with English abstract)
[魏新, 郑小锋, 张硕新 (2014) 秦岭火地塘不同海拔梯度森林土壤理化性质研究. 西北林学院学报, 29(3), 9-14.
43 Wu ZY, Lin WX, Chen ZF, Fang CX, Zhang ZX, Wu LK, Zhou MM, Shen LH (2013) Characteristics of soil microbial community under different vegetation types in Wuyishan National Nature Reserve, East China. Chinese Journal of Applied Ecology, 24, 2301-2309. (in Chinese with English abstract)
[吴则焰, 林文雄, 陈志芳, 方长旬, 张志兴, 吴林坤, 周明明, 沈荔花 (2013) 武夷山国家自然保护区不同植被类型土壤微生物群落特征. 应用生态学报, 24, 2301-2309.]
44 Wu ZY, Lin WX, Chen ZF, Liu JF, Fang CX, Zhang ZX, Wu LK, Chen T (2014) Phospholopid fatty acid analysis of soil microbes at different elevation of Wuyi Mountains. Scientia Silvae Sinicae, 50(7), 105-112. (in Chinese with English abstract)
[吴则焰, 林文雄, 陈志芳, 刘金福, 方长旬, 张志兴, 吴林坤, 陈婷 (2014) 武夷山不同海拔植被带土壤微生物PLFA分析. 林业科学, 50(7), 105-112.]
45 Xu SX, Shi HF, Zeng YP, Zhang FY, Wang ZH (1998) Biologic characteristics of Bacillus mycoides and application of radiation protection. Chinese Journal of Microecology, 10, 155-156, 163. (in Chinese with English abstract)
[徐书显, 时华富, 曾亚平, 张风云, 王忠海 (1998) 蕈状芽胞杆菌的特性及在辐射防护中的应用. 中国微生态学杂志, 10, 155-156, 163.]
46 Yazdani M, Naderi-Manesh H, Khajeh K, Soudi MR, Asghari SM, Sharifzadeh M (2009) Isolation and characterization of a novel gamma-radiation-resistant bacterium from hot spring in Iran. Journal of Basic Microbiology, 49, 119-127.
47 Yu ZN, Wang JP, He J (2013) Advance in genome research of Bacillus thuringiensis. Journal of Microbiology, 33(2), 1-6. (in Chinese with English abstract)
[喻子牛, 王阶平, 何进 (2013) 苏云金芽胞杆菌基因组研究. 微生物学杂志, 33(2), 1-6.]
48 Zhang FT, Huang HQ, Cui Y, Sun QG, Zhu J, Liu M, Bao SX (2014) Isolation and diversity of Bacillus species from Jiaxi tropical rain forest soil. Journal of Microbiology, 34(4), 42-46. (in Chinese with English abstract)
[张福特, 黄惠琴, 崔莹, 孙前光, 朱军, 刘敏, 鲍时翔 (2014) 佳西热带雨林土壤芽胞杆菌分离与多样性分析. 微生物学杂志, 34(4), 42-46.]
49 Zhang W, Wei HL, Gao HW, Hu YG (2005) Advances of studies on soil microbial diversity and environmental impact factors. Chinese Journal of Ecology, 24(1), 48-52. (in Chinese with English abstract)
[张薇, 魏海雷, 高洪文, 胡跃高 (2005) 土壤微生物多样性及其环境影响因子研究进展. 生态学杂志, 24(1), 48-52.]
50 Zhang WF, Quan JX, Xie L, Wang Q, Yi YT, Feng MM, Zhu L, Wang RP, Fang XJ (2009) Collection of Bacillus and identification of Bacillus thuringensis isolates from tropical rain forest reserves of Hainan Island. Genomics and Applied Biology, 28, 265-274. (in Chinese with English abstract)
[张文飞, 全嘉新, 谢柳, 王茜, 易艳桃, 丰玫玫, 朱麟, 王锐萍, 方宣钧 (2009) 海南岛热带雨林区芽胞杆菌收集及Bt菌鉴定. 基因组学与应用生物学, 28, 265-274.]
51 Zhang Y, Cong J, Lu H, Li G, Qu Y, Su X, Zhou J, Li D (2014) Community structure and elevational diversity patterns of soil Acidobacteria. Journal of Environmental Sciences, 26, 1717-1724.
52 Zhang Y, Guo LD, Liu RJ (2003) Diversity and ecology of arbuscular mycorrhizal. Acta Phytoecologica Sinica, 27, 537-544. (in Chinese with English abstract)
[张英, 郭良栋, 刘润进 (2003) 都江堰地区丛枝菌根真菌多样性与生态研究. 植物生态学报, 27, 537-544.]
53 Zhang YG, Su XJ, Cong J, Chen Z, Lu H, Liu MC, Li DQ (2014) Variation of soil microbial community along elevation in the Shennongjia Mountain. Scientia Silvae Sinicae, 50(9), 161-166. (in Chinese with English abstract)
[张于光, 宿秀江, 丛静, 陈展, 卢慧, 刘敏超, 李迪强 (2014) 神农架土壤微生物群落的海拔梯度变化. 林业科学, 50(9), 161-166.]
54 Zhou QP, Huang HQ, Cui Y, Liu M, Sun QG, Bao SX (2015) Diversity analysis of culturable Bacillus-like species from Jianfengling tropical rain forest soil. Guangdong Agricultural Sciences, 42(2), 59-63. (in Chinese with English abstract)
[周秋平, 黄惠琴, 崔莹, 刘敏, 孙前光, 鲍时翔 (2015) 尖峰岭热带雨林土壤中可培养芽胞杆菌多样性分析. 广东农业科学, 42(2), 59-63.]
55 Zhou WC, Lin J, Xiao Q (2011) Study on species diversity of land seashell in Wuyi Mountain Nature Reserve. Journal of Fujian Forestry Science and Technology, 38(3), 1-7. (in Chinese with English abstract)
[周卫川, 林晶, 肖琼 (2011) 武夷山自然保护区陆生贝类物种多样性研究. 福建林业科技, 38(3), 1-7.]
56 Zhuang TC, Lin P, Chen RH (1997) Amount and group of soil heterotrophic microorganisms in different forest types of Wuyi Mountains. Journal of Xiamen University (Natural Science), 36, 293-298. (in Chinese with English abstract)
[庄铁成, 林鹏, 陈仁华 (1997) 武夷山不同森林类型土壤异养微生物数量与类群组成. 厦门大学学报(自然科学版), 36, 293-298.]
[1] 李熠 唐志尧 闫昱晶 王科 蔡磊 贺金生 古松 姚一建. (2020) 物种分布模型在大型真菌红色名录评估及保护中的应用: 以冬虫夏草为例(大型真菌红色名录专辑). 生物多样性, 28(1): 0-0.
[2] 庄文颖 李熠 郑焕娣 曾昭清 王新存. (2020) 中国大型子囊菌受威胁现状及致危因素分析(大型真菌红色名录专辑). 生物多样性, 28(1): 0-0.
[3] 李顺, 邹亮, 宫一男, 杨海涛, 王天明, 冯利民, 葛剑平. (2019) 激光雷达技术在动物生态学领域的研究进展. 生物多样性, 27(9): 1021-1031.
[4] 杨锐, 彭钦一, 曹越, 钟乐, 侯姝彧, 赵智聪, 黄澄. (2019) 中国生物多样性保护的变革性转变及路径. 生物多样性, 27(9): 1032-1040.
[5] 谭一波, 申文辉, 付孜, 郑威, 欧芷阳, 谭长强, 彭玉华, 庞世龙, 何琴飞, 黄小荣, 何峰. (2019) 环境因子对桂西南蚬木林下植被物种多样性变异的解释. 生物多样性, 27(9): 970-983.
[6] 许金石,柴永福,刘晓,岳明,郭垚鑫,康慕谊,刘全儒,郑成洋,吉成均,闫明,张峰,高贤明,王仁卿,石福臣,张钦弟,王茂. (2019) 华北区域环境梯度上阔叶林构建模式及分布成因. 植物生态学报, 43(9): 732-741.
[7] 秦浩,张殷波,董刚,张峰. (2019) 山西关帝山森林群落物种、谱系和功能多样性海拔格局. 植物生态学报, 43(9): 762-773.
[8] 施晶晶,赵鸣飞,王宇航,薛峰,康慕谊,江源. (2019) 黄土高原腹地人工林下草本层群落构建机制. 植物生态学报, 43(9): 834-842.
[9] 唐丽丽,杨彤,刘鸿雁,康慕谊,王仁卿,张峰,高贤明,岳明,张梅,郑璞帆,石福臣. (2019) 华北地区荆条灌丛分布及物种多样性空间分异 规律. 植物生态学报, 43(9): 825-833.
[10] 李永民, 吴孝兵. (2019) 安徽省两栖爬行动物名录修订. 生物多样性, 27(9): 1002-1011.
[11] 方文静,蔡琼,朱江玲,吉成均,岳明,郭卫华,张峰,高贤明,唐志尧,方精云. (2019) 华北地区落叶松林的分布、群落结构和物种多样性. 植物生态学报, 43(9): 742-752.
[12] 陈自宏, 王元兵, 代永东, 陈凯, 徐玲, 何謦成. (2019) 滇西太保山森林公园子囊菌门虫生真菌物种多样性及其消长动态. 生物多样性, 27(9): 993-1001.
[13] 刘君, 王宁, 崔岱宗, 卢磊, 赵敏. (2019) 小兴安岭大亮子河国家森林公园不同生境下土壤细菌多样性和群落结构. 生物多样性, 27(8): 911-918.
[14] 吕美林, 刘泽, 宋震, 王亚宁, 刘小勇. (2019) 大兴安岭地区可培养毛霉门真菌多样性与分布. 生物多样性, 27(8): 821-832.
[15] 刘君, 王宁, 崔岱宗, 卢磊, 赵敏. (2019) 大小兴安岭可培养细菌的资源多样性. 生物多样性, 27(8): 903-910.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed