生物多样性 ›› 2010, Vol. 18 ›› Issue (2): 145-149.DOI: 10.3724/SP.J.1003.2010.150

• 论文 • 上一篇    下一篇

基于SRAP分子标记的海南沼虾种群遗传多样性

傅洪拓1,2*; 乔慧2; 姚建华2; 龚永生1; 吴滟1; 蒋速飞1; 熊贻伟1   

  1. 1 中国水产科学研究院淡水渔业研究中心, 农业部水生动物遗传育种和养殖生物学重点开放实验室, 江苏无锡 214081
    2 南京农业大学无锡渔业学院, 江苏无锡 214081
  • 收稿日期:2009-07-07 出版日期:2010-03-20 发布日期:2010-03-20
  • 通讯作者: 傅洪拓

Genetic diversity in five Macrobrachium hainanense populations using SRAP markers

Hongtuo Fu1, 2; Hui Qiao2; Jianhua Yao2; Yongsheng Gong1; Yan Wu1; Sufei Jiang1; Yiwei Xiong1   

  1. 1 Freshwater Fishery Research Center of Chinese Academy of Fishery Sciences, Key Open Laboratory for Genetic Breeding of Aquatic Animals and Aquaculture Biology, Ministry of Agriculture, Wuxi, Jiangsu 214081

    2 Wuxi Fishery College, Nanjing Agricultural University, Wuxi, Jiangsu 214081
  • Received:2009-07-07 Online:2010-03-20 Published:2010-03-20
  • Contact: Hongtuo Fu

摘要:

为了调查我国海南沼虾(Macrobrachium hainanense)不同地理种群遗传多样性及遗传分化状况, 本文采用序列相关扩增多态性(sequence-related amplified polymorphism, SRAP)标记对我国南方瓯江(OJ)、闽江(MJ)、珠江(PR)、万泉河(WQ)、昌化江(CH)等5个海南沼虾种群的遗传多样性进行了研究。共得到255个清晰、稳定的位点, 平均多态位点比例为47.05%, 由小到大依次为: PR (43.92%) = WQ (43.92%) < MJ (46.67%) < CH (47.45%) < OJ (53.33%)。遗传杂合度由小到大依次为: PR (0.1657) < WQ (0.1763) < CH (0.1799) < OJ (0.1839) < MJ (0.1892), 平均为0.1790。Shannon信息指数由小到大依次为: PR (0.2543) < WQ (0.2658) < CH (0.2746) < MJ (0.2846) < OJ (0.2876), 平均为0.2734。AMOVA分析结果显示, 17.64%的遗传变异来自种群间, 对总遗传变异影响显著(P<0.001)。遗传分化指数(Gst)、基因流(Nm)、遗传距离和聚类分析结果均显示5个海南沼虾种群间已经出现较大的遗传分化。

Abstract:

We studied the genetic variation and differentiation of five wild populations of Macrobrachium hainanense using SRAP markers. Samples were collected from Oujiang River (OJ), Minjiang River (MJ), Pearl River (PR), Wanquan River (WQ) and Changhuajiang River (CH). A total of 255 loci were detected, of which 47.05% were polymorphic. Proportion of polymorphic loci of the five populations ranked in the order: PR (43.92%) = WQ (43.92%) < MJ (46.67%) < CH (47.45%) < OJ (53.33%), while heterozygosity ranked as PR (0.1657) < WQ (0.1763) < CH (0.1799) < OJ (0.1839) < MJ (0.1892), with an average of 0.1790. Shannon index ranked as PR (0.2543) < WQ (0.2658) < CH (0.2746) < MJ (0.2846) < OJ (0.2876), with an average of 0.2734. Analysis of molecular variation (AMOVA) revealed that 17.64% of the variance was among populations, which significantly affected the total variance (P <0.001). Values of Gst, Nm and genetic distance suggested the genetic divergence among populations was relatively high (P < 0.001).