生物多样性 ›› 2008, Vol. 16 ›› Issue (6): 607-612.DOI: 10.3724/SP.J.1003.2008.08179

• 论文 • 上一篇    下一篇

福建省家鸭的遗传多样性及其与野鸭的亲缘关系

李慧芳1*, 徐文娟1, 朱文奇1, 薛素美2, 陈宽维1   

  1. 1 (中国农业科学院家禽研究所, 扬州 225003)
    2 (福建农林大学动物科学学院, 福州 350002)
  • 出版日期:2008-11-20 发布日期:2008-11-20
  • 通讯作者: 李慧芳

Genetic diversity and evolution of Fujian domestic duck breeds

Huifang Li1*, Wenjuan Xu1, Wenqi Zhu1, Sumei Xue2, Kuanwei Chen1   

  1. 1 Institute of Poultry Science, Chinese Academy of Agricultural Sciences, Yangzhou 225003
    2 College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou 350002
  • Online:2008-11-20 Published:2008-11-20
  • Contact: Huifang Li

摘要: 为了探讨福建省家鸭的遗传多样性和品种进化, 本研究测定了福建省4个地方家鸭品种的32个个体和野生斑嘴鸭(Anas zonorhyncha) 8个个体的线粒体DNA D-loop区667 bp序列, 分析了这4个家鸭品种的遗传多态性及品种进化。结果显示4个家鸭D-loop区序列的A、G、C、T碱基平均含量分别为25.5%, 15.2%, 33.5%, 25.8%。确定了8种单倍型, 其中Hap2(与GenBank中的A7一致)为家鸭的主体单倍型, 平均单倍型多样度为0.75202, 平均核苷酸多样度为0.21%。4个品种中金定鸭单倍型多样度、核苷酸多样度最高。4个品种之间的Kimura双参数遗传距离为0.00094–0.00395, 其中连城白鸭和金定鸭之间遗传距离最大。结合GenBank上已发表的绿头鸭(A. platyrhynchos)和斑嘴鸭序列, 共有30种单倍型, 共同构建系统发生树和网络关系图, 结果表明4个家鸭品种单一地起源于绿头鸭。

Abstract: To study the genetic diversity and evolution of four breeds of Fujian domestic ducks, the 667 bp control region (D-loop) of mitochondrial DNA from 32 individual domestic ducks and eight spot-billed ducks (Anas zonorhyncha) were sequenced. The results showed that in the D-loop region of four domestic duck breeds, the content of A, G, C and T nucleotides was 25.5%, 15.2%, 33.5% and 25.8%, respectively. Eight haplotypes were found, in which Hap2 (consistent with haplotype A7 in GenBank) was dominant. The average haplotype diversity (Hd) and nucleotide diversity (Pi) of domestic ducks were 0.75202 and 0.21%, respectively. The Hd and Pi of Jinding duck was the highest among the four domestic duck breeds. Kimura 2-parameter genetic distance among the four duck breeds were 0.00094–0.00395. Combined with the sequences of mallard (A. platyrhynchos) and spot-billed ducks taken from GenBank, a total of 30 haplotypes were used in the phylogenetic analysis, and the results showed that the four domestic duck breeds originated from A. platyrhynchos.